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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 130 |
| AccB1I | GGYRCC | 2 cut(s) | 130, 358 |
| AccII | CGCG | 3 cut(s) | 49, 108, 667 |
| AciI | CCGC | 4 cut(s) | 106, 108, 368, 667 |
| AclWI | GGATC | 2 cut(s) | 131, 732 |
| AcoI | YGGCCR | 2 cut(s) | 501, 517 |
| AcsI | RAATTY | 1 cut(s) | 708 |
| AcuI | CTGAAG | 1 cut(s) | 246 |
| AcyI | GRCGYC | 1 cut(s) | 75 |
| AdeI | CACNNNGTG | 1 cut(s) | 265 |
| AfaI | GTAC | 2 cut(s) | 132, 447 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 262, 666, 667 |
| AgsI | TTSAA | 2 cut(s) | 146, 187 |
| AluBI | AGCT | 1 cut(s) | 352 |
| AluI | AGCT | 1 cut(s) | 352 |
| Alw26I | GTCTC | 1 cut(s) | 246 |
| AlwI | GGATC | 2 cut(s) | 131, 732 |
| Ama87I | CYCGRG | 1 cut(s) | 485 |
| AoxI | GGCC | 5 cut(s) | 109, 404, 501, 517, 686 |
| ApeKI | GCWGC | 1 cut(s) | 99 |
| ApoI | RAATTY | 1 cut(s) | 708 |
| AseI | ATTAAT | 1 cut(s) | 626 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 678 |
| Asp718I | GGTACC | 1 cut(s) | 130 |
| AspLEI | GCGC | 2 cut(s) | 49, 51 |
| AspS9I | GGNCC | 2 cut(s) | 404, 686 |
| AsuHPI | GGTGA | 1 cut(s) | 505 |
| AvaI | CYCGRG | 1 cut(s) | 485 |
| BaeGI | GKGCMC | 1 cut(s) | 363 |
| BalI | TGGCCA | 1 cut(s) | 503 |
| BanI | GGYRCC | 2 cut(s) | 130, 358 |
| BbvI | GCAGC | 1 cut(s) | 86 |
| BccI | CCATC | 1 cut(s) | 27 |
| BceAI | ACGGC | 2 cut(s) | 62, 524 |
| BcoDI | GTCTC | 1 cut(s) | 246 |
| BfaI | CTAG | 1 cut(s) | 528 |
| BisI | GCNGC | 2 cut(s) | 100, 109 |
| BlsI | GCNGC | 2 cut(s) | 101, 110 |
| BmeT110I | CYCGRG | 1 cut(s) | 485 |
| BmgT120I | GGNCC | 2 cut(s) | 404, 686 |
| BmiI | GGNNCC | 3 cut(s) | 132, 360, 405 |
| BmsI | GCATC | 2 cut(s) | 34, 550 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 196, 228 |
| BpuEI | CTTGAG | 1 cut(s) | 74 |
| BsaHI | GRCGYC | 1 cut(s) | 75 |
| BsaI | GGTCTC | 1 cut(s) | 246 |
| BsaJI | CCNNGG | 2 cut(s) | 106, 540 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 262, 666, 667 |
| Bse118I | RCCGGY | 1 cut(s) | 515 |
| Bse1I | ACTGG | 2 cut(s) | 504, 504 |
| BseDI | CCNNGG | 2 cut(s) | 106, 540 |
| BseGI | GGATG | 1 cut(s) | 19 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 262, 666, 667 |
| BseNI | ACTGG | 2 cut(s) | 504, 504 |
| BsePI | GCGCGC | 1 cut(s) | 47 |
| BseRI | GAGGAG | 1 cut(s) | 338 |
| BseSI | GKGCMC | 1 cut(s) | 363 |
| BseXI | GCAGC | 1 cut(s) | 86 |
| Bsh1236I | CGCG | 3 cut(s) | 49, 108, 667 |
| Bsh1285I | CGRYCG | 1 cut(s) | 337 |
| BshFI | GGCC | 5 cut(s) | 111, 406, 503, 519, 688 |
| BshNI | GGYRCC | 2 cut(s) | 130, 358 |
| BsiEI | CGRYCG | 1 cut(s) | 337 |
| BsiHKCI | CYCGRG | 1 cut(s) | 485 |
| BsiSI | CCGG | 2 cut(s) | 96, 516 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 262, 666, 667 |
| BsmAI | GTCTC | 1 cut(s) | 246 |
| BsmI | GAATGC | 1 cut(s) | 553 |
| BsnI | GGCC | 5 cut(s) | 111, 406, 503, 519, 688 |
| Bso31I | GGTCTC | 1 cut(s) | 246 |
| BsoBI | CYCGRG | 1 cut(s) | 485 |
| Bsp1286I | GDGCHC | 1 cut(s) | 363 |
| Bsp1407I | TGTACA | 1 cut(s) | 445 |
| Bsp143I | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| BspACI | CCGC | 4 cut(s) | 106, 108, 368, 667 |
| BspANI | GGCC | 5 cut(s) | 111, 406, 503, 519, 688 |
| BspFNI | CGCG | 3 cut(s) | 49, 108, 667 |
| BspLI | GGNNCC | 3 cut(s) | 132, 360, 405 |
| BspPI | GGATC | 2 cut(s) | 131, 732 |
| BspT107I | GGYRCC | 2 cut(s) | 130, 358 |
| BspTNI | GGTCTC | 1 cut(s) | 246 |
| BsrFI | RCCGGY | 1 cut(s) | 515 |
| BsrGI | TGTACA | 1 cut(s) | 445 |
| BsrI | ACTGG | 2 cut(s) | 504, 504 |
| BssAI | RCCGGY | 1 cut(s) | 515 |
| BssECI | CCNNGG | 2 cut(s) | 106, 540 |
| BssHII | GCGCGC | 1 cut(s) | 47 |
| BssMI | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| BssNI | GRCGYC | 1 cut(s) | 75 |
| Bst4CI | ACNGT | 1 cut(s) | 265 |
| Bst6I | CTCTTC | 2 cut(s) | 31, 119 |
| BstACI | GRCGYC | 1 cut(s) | 75 |
| BstAUI | TGTACA | 1 cut(s) | 445 |
| BstC8I | GCNNGC | 2 cut(s) | 49, 702 |
| BstDSI | CCRYGG | 2 cut(s) | 106, 540 |
| BstEII | GGTNACC | 1 cut(s) | 511 |
| BstF5I | GGATG | 1 cut(s) | 19 |
| BstFNI | CGCG | 3 cut(s) | 49, 108, 667 |
| BstHHI | GCGC | 2 cut(s) | 49, 51 |
| BstKTI | GATC | 9 cut(s) | 85, 126, 140, 330, 337, 567, 722, 727, 735 |
| BstMAI | GTCTC | 1 cut(s) | 246 |
| BstMBI | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| BstMCI | CGRYCG | 1 cut(s) | 337 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 105, 108, 358 |
| BstPI | GGTNACC | 1 cut(s) | 511 |
| BstSLI | GKGCMC | 1 cut(s) | 363 |
| BstUI | CGCG | 3 cut(s) | 49, 108, 667 |
| BstV1I | GCAGC | 1 cut(s) | 86 |
| BstX2I | RGATCY | 1 cut(s) | 724 |
| BstYI | RGATCY | 1 cut(s) | 724 |
| BsuRI | GGCC | 5 cut(s) | 111, 406, 503, 519, 688 |
| BtgI | CCRYGG | 2 cut(s) | 106, 540 |
| BtsCI | GGATG | 1 cut(s) | 19 |
| BtsIMutI | CAGTG | 1 cut(s) | 511 |
| Cac8I | GCNNGC | 2 cut(s) | 49, 702 |
| CfoI | GCGC | 2 cut(s) | 49, 51 |
| Cfr10I | RCCGGY | 1 cut(s) | 515 |
| Cfr13I | GGNCC | 2 cut(s) | 404, 686 |
| Cfr42I | CCGCGG | 1 cut(s) | 109 |
| CseI | GACGC | 2 cut(s) | 71, 83 |
| Csp6I | GTAC | 2 cut(s) | 131, 446 |
| CviAII | CATG | 3 cut(s) | 152, 449, 683 |
| CviQI | GTAC | 2 cut(s) | 131, 446 |
| DpnI | GATC | 9 cut(s) | 84, 125, 139, 329, 336, 566, 721, 726, 734 |
| DpnII | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| DraIII | CACNNNGTG | 1 cut(s) | 265 |
| EaeI | YGGCCR | 2 cut(s) | 501, 517 |
| Eam1104I | CTCTTC | 2 cut(s) | 31, 119 |
| EarI | CTCTTC | 2 cut(s) | 31, 119 |
| Eco31I | GGTCTC | 1 cut(s) | 246 |
| Eco57I | CTGAAG | 1 cut(s) | 246 |
| Eco88I | CYCGRG | 1 cut(s) | 485 |
| Eco91I | GGTNACC | 1 cut(s) | 511 |
| EcoO109I | RGGNCCY | 1 cut(s) | 404 |
| EcoO65I | GGTNACC | 1 cut(s) | 511 |
| FaeI | CATG | 3 cut(s) | 155, 452, 686 |
| FatI | CATG | 3 cut(s) | 151, 448, 682 |
| FauI | CCCGC | 1 cut(s) | 660 |
| Fnu4HI | GCNGC | 2 cut(s) | 100, 109 |
| FokI | GGATG | 1 cut(s) | 6 |
| Fsp4HI | GCNGC | 2 cut(s) | 100, 109 |
| FspBI | CTAG | 1 cut(s) | 528 |
| GlaI | GCGC | 2 cut(s) | 48, 50 |
| GluI | GCNGC | 2 cut(s) | 100, 109 |
| HaeIII | GGCC | 5 cut(s) | 111, 406, 503, 519, 688 |
| HapII | CCGG | 2 cut(s) | 96, 516 |
| HgaI | GACGC | 2 cut(s) | 71, 83 |
| HhaI | GCGC | 2 cut(s) | 49, 51 |
| Hin1I | GRCGYC | 1 cut(s) | 75 |
| Hin1II | CATG | 3 cut(s) | 155, 452, 686 |
| Hin6I | GCGC | 2 cut(s) | 47, 49 |
| HinP1I | GCGC | 2 cut(s) | 47, 49 |
| HinfI | GANTC | 2 cut(s) | 146, 234 |
| HpaII | CCGG | 2 cut(s) | 96, 516 |
| HphI | GGTGA | 1 cut(s) | 505 |
| Hpy188I | TCNGA | 3 cut(s) | 123, 243, 724 |
| Hpy188III | TCNNGA | 1 cut(s) | 487 |
| Hpy99I | CGWCG | 3 cut(s) | 65, 77, 335 |
| HpyAV | CCTTC | 3 cut(s) | 221, 584, 598 |
| HpyCH4III | ACNGT | 1 cut(s) | 265 |
| HpyCH4IV | ACGT | 1 cut(s) | 692 |
| HpyCH4V | TGCA | 3 cut(s) | 465, 556, 645 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 105, 108, 358 |
| HpySE526I | ACGT | 1 cut(s) | 692 |
| Hsp92I | GRCGYC | 1 cut(s) | 75 |
| Hsp92II | CATG | 3 cut(s) | 155, 452, 686 |
| HspAI | GCGC | 2 cut(s) | 47, 49 |
| KpnI | GGTACC | 1 cut(s) | 134 |
| KspI | CCGCGG | 1 cut(s) | 109 |
| Kzo9I | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| LmnI | GCTCC | 1 cut(s) | 21 |
| LpnPI | CCDG | 7 cut(s) | 109, 338, 485, 517, 529, 620, 714 |
| Lsp1109I | GCAGC | 1 cut(s) | 86 |
| LweI | GCATC | 2 cut(s) | 34, 550 |
| MaeI | CTAG | 1 cut(s) | 528 |
| MaeII | ACGT | 1 cut(s) | 692 |
| MaeIII | GTNAC | 1 cut(s) | 511 |
| MalI | GATC | 9 cut(s) | 84, 125, 139, 329, 336, 566, 721, 726, 734 |
| MboI | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| MboII | GAAGA | 6 cut(s) | 18, 106, 358, 398, 401, 613 |
| MflI | RGATCY | 1 cut(s) | 724 |
| MhlI | GDGCHC | 1 cut(s) | 363 |
| MlsI | TGGCCA | 1 cut(s) | 503 |
| MluCI | AATT | 4 cut(s) | 180, 298, 677, 708 |
| MluNI | TGGCCA | 1 cut(s) | 503 |
| MlyI | GAGTC | 1 cut(s) | 243 |
| MmeI | TCCRAC | 2 cut(s) | 602, 729 |
| Mox20I | TGGCCA | 1 cut(s) | 503 |
| MroXI | GAANNNNTTC | 1 cut(s) | 678 |
| MscI | TGGCCA | 1 cut(s) | 503 |
| MseI | TTAA | 4 cut(s) | 179, 378, 626, 760 |
| Msp20I | TGGCCA | 1 cut(s) | 503 |
| MspA1I | CMGCKG | 1 cut(s) | 108 |
| MspI | CCGG | 2 cut(s) | 96, 516 |
| Mva1269I | GAATGC | 1 cut(s) | 553 |
| MvnI | CGCG | 3 cut(s) | 49, 108, 667 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 105, 108, 358 |
| NdeII | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| NlaIII | CATG | 3 cut(s) | 155, 452, 686 |
| NlaIV | GGNNCC | 3 cut(s) | 132, 360, 405 |
| NmuCI | GTSAC | 1 cut(s) | 511 |
| PaeR7I | CTCGAG | 1 cut(s) | 485 |
| PauI | GCGCGC | 1 cut(s) | 47 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 69 |
| PctI | GAATGC | 1 cut(s) | 553 |
| PdmI | GAANNNNTTC | 1 cut(s) | 678 |
| PfeI | GAWTC | 1 cut(s) | 146 |
| PkrI | GCNGC | 2 cut(s) | 101, 110 |
| Ple19I | CGATCG | 1 cut(s) | 337 |
| PleI | GAGTC | 1 cut(s) | 242 |
| PpsI | GAGTC | 1 cut(s) | 242 |
| PshBI | ATTAAT | 1 cut(s) | 626 |
| PspEI | GGTNACC | 1 cut(s) | 511 |
| PspN4I | GGNNCC | 3 cut(s) | 132, 360, 405 |
| PspPI | GGNCC | 2 cut(s) | 404, 686 |
| PsuI | RGATCY | 1 cut(s) | 724 |
| PteI | GCGCGC | 1 cut(s) | 47 |
| PvuI | CGATCG | 1 cut(s) | 337 |
| RsaI | GTAC | 2 cut(s) | 132, 447 |
| RsaNI | GTAC | 2 cut(s) | 131, 446 |
| SacII | CCGCGG | 1 cut(s) | 109 |
| SaqAI | TTAA | 4 cut(s) | 179, 378, 626, 760 |
| SatI | GCNGC | 2 cut(s) | 100, 109 |
| Sau3AI | GATC | 9 cut(s) | 82, 123, 137, 327, 334, 564, 719, 724, 732 |
| Sau96I | GGNCC | 2 cut(s) | 404, 686 |
| SchI | GAGTC | 1 cut(s) | 243 |
| SduI | GDGCHC | 1 cut(s) | 363 |
| SetI | ASST | 7 cut(s) | 132, 179, 192, 232, 354, 513, 695 |
| SfaNI | GCATC | 2 cut(s) | 34, 550 |
| Sfr274I | CTCGAG | 1 cut(s) | 485 |
| Sfr303I | CCGCGG | 1 cut(s) | 109 |
| SgrBI | CCGCGG | 1 cut(s) | 109 |
| SlaI | CTCGAG | 1 cut(s) | 485 |
| SmlI | CTYRAG | 2 cut(s) | 89, 485 |
| SmoI | CTYRAG | 2 cut(s) | 89, 485 |
| Sse9I | AATT | 4 cut(s) | 180, 298, 677, 708 |
| SsiI | CCGC | 4 cut(s) | 106, 108, 368, 667 |
| SspMI | CTAG | 1 cut(s) | 528 |
| TaaI | ACNGT | 1 cut(s) | 265 |
| TaiI | ACGT | 1 cut(s) | 695 |
| TaqI | TCGA | 4 cut(s) | 126, 285, 333, 486 |
| TaqII | GACCGA | 1 cut(s) | 270 |
| TasI | AATT | 4 cut(s) | 180, 298, 677, 708 |
| TatI | WGTACW | 1 cut(s) | 445 |
| TauI | GCSGC | 1 cut(s) | 111 |
| TfiI | GAWTC | 1 cut(s) | 146 |
| Tru1I | TTAA | 4 cut(s) | 179, 378, 626, 760 |
| Tru9I | TTAA | 4 cut(s) | 179, 378, 626, 760 |
| TscAI | CASTG | 1 cut(s) | 511 |
| TseFI | GTSAC | 1 cut(s) | 511 |
| TseI | GCWGC | 1 cut(s) | 99 |
| Tsp45I | GTSAC | 1 cut(s) | 511 |
| TspDTI | ATGAA | 2 cut(s) | 509, 671 |
| TspRI | CASTG | 1 cut(s) | 511 |
| VspI | ATTAAT | 1 cut(s) | 626 |
| XapI | RAATTY | 1 cut(s) | 708 |
| XhoI | CTCGAG | 1 cut(s) | 485 |
| XmnI | GAANNNNTTC | 1 cut(s) | 678 |
| XspI | CTAG | 1 cut(s) | 528 |