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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 372, 380 |
| AclWI | GGATC | 1 cut(s) | 138 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 226 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 292 |
| AflIII | ACRYGT | 2 cut(s) | 48, 92 |
| AgsI | TTSAA | 2 cut(s) | 116, 124 |
| AjuI | GAANNNNNNNTTGG | 1 cut(s) | 31 |
| AluBI | AGCT | 2 cut(s) | 191, 329 |
| AluI | AGCT | 2 cut(s) | 191, 329 |
| AlwI | GGATC | 1 cut(s) | 138 |
| Ama87I | CYCGRG | 1 cut(s) | 367 |
| AoxI | GGCC | 2 cut(s) | 3, 27 |
| ApoI | RAATTY | 1 cut(s) | 226 |
| AsuC2I | CCSGG | 1 cut(s) | 290 |
| AsuHPI | GGTGA | 1 cut(s) | 314 |
| AvaI | CYCGRG | 1 cut(s) | 367 |
| BalI | TGGCCA | 1 cut(s) | 5 |
| BccI | CCATC | 2 cut(s) | 263, 299 |
| BclI | TGATCA | 1 cut(s) | 220 |
| BcnI | CCSGG | 1 cut(s) | 290 |
| BfaI | CTAG | 1 cut(s) | 251 |
| Bme1390I | CCNGG | 1 cut(s) | 290 |
| BmeT110I | CYCGRG | 1 cut(s) | 367 |
| BmrFI | CCNGG | 1 cut(s) | 290 |
| BmsI | GCATC | 1 cut(s) | 364 |
| BpuMI | CCSGG | 1 cut(s) | 290 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 292 |
| Bse1I | ACTGG | 1 cut(s) | 30 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 292 |
| BseNI | ACTGG | 1 cut(s) | 30 |
| BseRI | GAGGAG | 1 cut(s) | 175 |
| Bsh1285I | CGRYCG | 1 cut(s) | 319 |
| BshFI | GGCC | 2 cut(s) | 5, 29 |
| BsiEI | CGRYCG | 1 cut(s) | 319 |
| BsiHKCI | CYCGRG | 1 cut(s) | 367 |
| BsiSI | CCGG | 1 cut(s) | 289 |
| BslFI | GGGAC | 1 cut(s) | 309 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 292 |
| BsmFI | GGGAC | 1 cut(s) | 309 |
| BsnI | GGCC | 2 cut(s) | 5, 29 |
| BsoBI | CYCGRG | 1 cut(s) | 367 |
| Bsp143I | GATC | 4 cut(s) | 130, 169, 220, 316 |
| BspACI | CCGC | 2 cut(s) | 372, 380 |
| BspANI | GGCC | 2 cut(s) | 5, 29 |
| BspPI | GGATC | 1 cut(s) | 138 |
| BsrI | ACTGG | 1 cut(s) | 30 |
| BssMI | GATC | 4 cut(s) | 130, 169, 220, 316 |
| Bst6I | CTCTTC | 1 cut(s) | 152 |
| BstKTI | GATC | 4 cut(s) | 133, 172, 223, 319 |
| BstMBI | GATC | 4 cut(s) | 130, 169, 220, 316 |
| BstMCI | CGRYCG | 1 cut(s) | 319 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 188 |
| BstNSI | RCATGY | 4 cut(s) | 52, 76, 96, 336 |
| BstSCI | CCNGG | 1 cut(s) | 288 |
| BsuRI | GGCC | 2 cut(s) | 5, 29 |
| BtgZI | GCGATG | 1 cut(s) | 53 |
| CviAII | CATG | 7 cut(s) | 19, 49, 73, 93, 299, 333, 344 |
| CviJI | RGCY | 5 cut(s) | 5, 29, 191, 329, 366 |
| CviKI_1 | RGCY | 5 cut(s) | 5, 29, 191, 329, 366 |
| DpnI | GATC | 4 cut(s) | 132, 171, 222, 318 |
| DpnII | GATC | 4 cut(s) | 130, 169, 220, 316 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 1 cut(s) | 152 |
| EarI | CTCTTC | 1 cut(s) | 152 |
| EciI | GGCGGA | 1 cut(s) | 387 |
| Eco88I | CYCGRG | 1 cut(s) | 367 |
| EcoRI | GAATTC | 1 cut(s) | 226 |
| FaeI | CATG | 7 cut(s) | 22, 52, 76, 96, 302, 336, 347 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 31 |
| FaqI | GGGAC | 1 cut(s) | 309 |
| FatI | CATG | 7 cut(s) | 18, 48, 72, 92, 298, 332, 343 |
| FauNDI | CATATG | 1 cut(s) | 85 |
| FbaI | TGATCA | 1 cut(s) | 220 |
| FspBI | CTAG | 1 cut(s) | 251 |
| HaeIII | GGCC | 2 cut(s) | 5, 29 |
| HapII | CCGG | 1 cut(s) | 289 |
| Hin1II | CATG | 7 cut(s) | 22, 52, 76, 96, 302, 336, 347 |
| HincII | GTYRAC | 1 cut(s) | 78 |
| HindII | GTYRAC | 1 cut(s) | 78 |
| HinfI | GANTC | 3 cut(s) | 140, 146, 375 |
| HpaII | CCGG | 1 cut(s) | 289 |
| HphI | GGTGA | 1 cut(s) | 314 |
| Hpy166II | GTNNAC | 2 cut(s) | 46, 78 |
| Hpy188I | TCNGA | 5 cut(s) | 105, 145, 151, 196, 225 |
| Hpy188III | TCNNGA | 3 cut(s) | 134, 173, 275 |
| Hpy8I | GTNNAC | 2 cut(s) | 46, 78 |
| HpyAV | CCTTC | 3 cut(s) | 40, 121, 191 |
| HpyCH4V | TGCA | 1 cut(s) | 355 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 188 |
| Hsp92II | CATG | 7 cut(s) | 22, 52, 76, 96, 302, 336, 347 |
| Ksp22I | TGATCA | 1 cut(s) | 220 |
| Kzo9I | GATC | 4 cut(s) | 130, 169, 220, 316 |
| LmnI | GCTCC | 1 cut(s) | 326 |
| LpnPI | CCDG | 2 cut(s) | 11, 302 |
| LweI | GCATC | 1 cut(s) | 364 |
| MaeI | CTAG | 1 cut(s) | 251 |
| MaeIII | GTNAC | 3 cut(s) | 9, 21, 63 |
| MalI | GATC | 4 cut(s) | 132, 171, 222, 318 |
| MboI | GATC | 4 cut(s) | 130, 169, 220, 316 |
| MboII | GAAGA | 7 cut(s) | 136, 169, 188, 218, 251, 254, 275 |
| MlsI | TGGCCA | 1 cut(s) | 5 |
| MluCI | AATT | 1 cut(s) | 226 |
| MluNI | TGGCCA | 1 cut(s) | 5 |
| MlyI | GAGTC | 1 cut(s) | 369 |
| MnlI | CCTC | 6 cut(s) | 130, 147, 153, 196, 253, 286 |
| Mox20I | TGGCCA | 1 cut(s) | 5 |
| MscI | TGGCCA | 1 cut(s) | 5 |
| MslI | CAYNNNNRTG | 1 cut(s) | 303 |
| Msp20I | TGGCCA | 1 cut(s) | 5 |
| MspI | CCGG | 1 cut(s) | 289 |
| MspR9I | CCNGG | 1 cut(s) | 290 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 188 |
| NciI | CCSGG | 1 cut(s) | 290 |
| NdeI | CATATG | 1 cut(s) | 85 |
| NdeII | GATC | 4 cut(s) | 130, 169, 220, 316 |
| NlaIII | CATG | 7 cut(s) | 22, 52, 76, 96, 302, 336, 347 |
| NmuCI | GTSAC | 2 cut(s) | 9, 63 |
| NspI | RCATGY | 4 cut(s) | 52, 76, 96, 336 |
| PciI | ACATGT | 2 cut(s) | 48, 92 |
| PfeI | GAWTC | 2 cut(s) | 140, 146 |
| Ple19I | CGATCG | 1 cut(s) | 319 |
| PleI | GAGTC | 1 cut(s) | 369 |
| PpsI | GAGTC | 1 cut(s) | 369 |
| PscI | ACATGT | 2 cut(s) | 48, 92 |
| PvuI | CGATCG | 1 cut(s) | 319 |
| RseI | CAYNNNNRTG | 1 cut(s) | 303 |
| Sau3AI | GATC | 4 cut(s) | 130, 169, 220, 316 |
| SchI | GAGTC | 1 cut(s) | 369 |
| ScrFI | CCNGG | 1 cut(s) | 290 |
| SetI | ASST | 3 cut(s) | 193, 252, 331 |
| SfaNI | GCATC | 1 cut(s) | 364 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 303 |
| Sse9I | AATT | 1 cut(s) | 226 |
| SsiI | CCGC | 2 cut(s) | 372, 380 |
| SspI | AATATT | 1 cut(s) | 111 |
| SspMI | CTAG | 1 cut(s) | 251 |
| StyD4I | CCNGG | 1 cut(s) | 288 |
| TaqI | TCGA | 2 cut(s) | 172, 315 |
| TasI | AATT | 1 cut(s) | 226 |
| TfiI | GAWTC | 2 cut(s) | 140, 146 |
| TseFI | GTSAC | 2 cut(s) | 9, 63 |
| Tsp45I | GTSAC | 2 cut(s) | 9, 63 |
| TspDTI | ATGAA | 3 cut(s) | 7, 22, 360 |
| XapI | RAATTY | 1 cut(s) | 226 |
| XceI | RCATGY | 4 cut(s) | 52, 76, 96, 336 |
| XspI | CTAG | 1 cut(s) | 251 |