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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 187 |
| AclWI | GGATC | 2 cut(s) | 172, 498 |
| AcsI | RAATTY | 1 cut(s) | 474 |
| AcuI | CTGAAG | 1 cut(s) | 123 |
| AfaI | GTAC | 1 cut(s) | 122 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 43, 187, 433 |
| AgsI | TTSAA | 1 cut(s) | 279 |
| AjiI | CACGTC | 1 cut(s) | 23 |
| AjnI | CCWGG | 1 cut(s) | 60 |
| AluBI | AGCT | 1 cut(s) | 271 |
| AluI | AGCT | 1 cut(s) | 271 |
| AlwI | GGATC | 2 cut(s) | 172, 498 |
| AoxI | GGCC | 1 cut(s) | 452 |
| ApeKI | GCWGC | 1 cut(s) | 268 |
| ApoI | RAATTY | 1 cut(s) | 474 |
| AseI | ATTAAT | 1 cut(s) | 392 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 444 |
| AspS9I | GGNCC | 2 cut(s) | 252, 452 |
| AsuNHI | GCTAGC | 1 cut(s) | 11 |
| AvaII | GGWCC | 1 cut(s) | 252 |
| BbvI | GCAGC | 1 cut(s) | 280 |
| BccI | CCATC | 1 cut(s) | 83 |
| BceAI | ACGGC | 1 cut(s) | 290 |
| BciT130I | CCWGG | 1 cut(s) | 62 |
| BfaI | CTAG | 2 cut(s) | 12, 38 |
| BisI | GCNGC | 1 cut(s) | 269 |
| BlsI | GCNGC | 1 cut(s) | 270 |
| Bme1390I | CCNGG | 1 cut(s) | 62 |
| Bme18I | GGWCC | 1 cut(s) | 252 |
| BmgBI | CACGTC | 1 cut(s) | 23 |
| BmgT120I | GGNCC | 2 cut(s) | 252, 452 |
| BmrFI | CCNGG | 1 cut(s) | 62 |
| BmsI | GCATC | 3 cut(s) | 88, 190, 316 |
| BmtI | GCTAGC | 1 cut(s) | 15 |
| Bsa29I | ATCGAT | 1 cut(s) | 198 |
| BsaBI | GATNNNNATC | 1 cut(s) | 176 |
| BsaJI | CCNNGG | 3 cut(s) | 162, 204, 306 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 43, 187, 433 |
| Bse1I | ACTGG | 1 cut(s) | 71 |
| Bse8I | GATNNNNATC | 1 cut(s) | 176 |
| BseBI | CCWGG | 1 cut(s) | 62 |
| BseCI | ATCGAT | 1 cut(s) | 198 |
| BseDI | CCNNGG | 3 cut(s) | 162, 204, 306 |
| BseJI | GATNNNNATC | 1 cut(s) | 176 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 43, 187, 433 |
| BseNI | ACTGG | 1 cut(s) | 71 |
| BseXI | GCAGC | 1 cut(s) | 280 |
| Bsh1285I | CGRYCG | 1 cut(s) | 127 |
| BshFI | GGCC | 1 cut(s) | 454 |
| BshVI | ATCGAT | 1 cut(s) | 198 |
| BsiEI | CGRYCG | 1 cut(s) | 127 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 43, 187, 433 |
| BsmI | GAATGC | 1 cut(s) | 319 |
| BsnI | GGCC | 1 cut(s) | 454 |
| Bsp143I | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| BspANI | GGCC | 1 cut(s) | 454 |
| BspDI | ATCGAT | 1 cut(s) | 198 |
| BspOI | GCTAGC | 1 cut(s) | 15 |
| BspPI | GGATC | 2 cut(s) | 172, 498 |
| BsrI | ACTGG | 1 cut(s) | 71 |
| BssECI | CCNNGG | 3 cut(s) | 162, 204, 306 |
| BssMI | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| BssT1I | CCWWGG | 2 cut(s) | 162, 204 |
| Bst2UI | CCWGG | 1 cut(s) | 62 |
| BstC8I | GCNNGC | 4 cut(s) | 13, 80, 222, 468 |
| BstDSI | CCRYGG | 1 cut(s) | 306 |
| BstKTI | GATC | 6 cut(s) | 127, 180, 333, 488, 493, 501 |
| BstMBI | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| BstMCI | CGRYCG | 1 cut(s) | 127 |
| BstNI | CCWGG | 1 cut(s) | 62 |
| BstSCI | CCNGG | 1 cut(s) | 60 |
| BstV1I | GCAGC | 1 cut(s) | 280 |
| BstX2I | RGATCY | 2 cut(s) | 177, 490 |
| BstYI | RGATCY | 2 cut(s) | 177, 490 |
| Bsu15I | ATCGAT | 1 cut(s) | 198 |
| BsuRI | GGCC | 1 cut(s) | 454 |
| BsuTUI | ATCGAT | 1 cut(s) | 198 |
| BtgI | CCRYGG | 1 cut(s) | 306 |
| BtrI | CACGTC | 1 cut(s) | 23 |
| Cac8I | GCNNGC | 4 cut(s) | 13, 80, 222, 468 |
| Cfr13I | GGNCC | 2 cut(s) | 252, 452 |
| ClaI | ATCGAT | 1 cut(s) | 198 |
| CpoI | CGGWCCG | 1 cut(s) | 252 |
| Csp6I | GTAC | 1 cut(s) | 121 |
| CspI | CGGWCCG | 1 cut(s) | 252 |
| CviAII | CATG | 1 cut(s) | 449 |
| CviJI | RGCY | 7 cut(s) | 150, 161, 220, 271, 305, 454, 466 |
| CviKI_1 | RGCY | 7 cut(s) | 150, 161, 220, 271, 305, 454, 466 |
| CviQI | GTAC | 1 cut(s) | 121 |
| DpnI | GATC | 6 cut(s) | 126, 179, 332, 487, 492, 500 |
| DpnII | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| Eco130I | CCWWGG | 2 cut(s) | 162, 204 |
| Eco32I | GATATC | 1 cut(s) | 58 |
| Eco47I | GGWCC | 1 cut(s) | 252 |
| Eco57I | CTGAAG | 1 cut(s) | 123 |
| EcoRII | CCWGG | 1 cut(s) | 60 |
| EcoRV | GATATC | 1 cut(s) | 58 |
| EcoT14I | CCWWGG | 2 cut(s) | 162, 204 |
| ErhI | CCWWGG | 2 cut(s) | 162, 204 |
| FaeI | CATG | 1 cut(s) | 452 |
| FaiI | YATR | 9 cut(s) | 17, 96, 113, 226, 228, 247, 413, 450, 497 |
| FatI | CATG | 1 cut(s) | 448 |
| Fnu4HI | GCNGC | 1 cut(s) | 269 |
| Fsp4HI | GCNGC | 1 cut(s) | 269 |
| FspBI | CTAG | 2 cut(s) | 12, 38 |
| GluI | GCNGC | 1 cut(s) | 269 |
| HaeIII | GGCC | 1 cut(s) | 454 |
| Hin1II | CATG | 1 cut(s) | 452 |
| HinfI | GANTC | 2 cut(s) | 70, 172 |
| Hpy188I | TCNGA | 4 cut(s) | 88, 177, 256, 490 |
| HpyAV | CCTTC | 1 cut(s) | 364 |
| HpyCH4IV | ACGT | 2 cut(s) | 22, 458 |
| HpyCH4V | TGCA | 4 cut(s) | 224, 245, 322, 411 |
| HpySE526I | ACGT | 2 cut(s) | 22, 458 |
| Hsp92II | CATG | 1 cut(s) | 452 |
| Kzo9I | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| LpnPI | CCDG | 8 cut(s) | 47, 52, 74, 92, 278, 386, 419, 480 |
| Lsp1109I | GCAGC | 1 cut(s) | 280 |
| LweI | GCATC | 3 cut(s) | 88, 190, 316 |
| MaeI | CTAG | 2 cut(s) | 12, 38 |
| MaeII | ACGT | 2 cut(s) | 22, 458 |
| MalI | GATC | 6 cut(s) | 126, 179, 332, 487, 492, 500 |
| MboI | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| MboII | GAAGA | 3 cut(s) | 65, 181, 379 |
| MflI | RGATCY | 2 cut(s) | 177, 490 |
| MluCI | AATT | 3 cut(s) | 291, 443, 474 |
| MmeI | TCCRAC | 3 cut(s) | 279, 368, 495 |
| MnlI | CCTC | 4 cut(s) | 232, 346, 430, 500 |
| MroXI | GAANNNNTTC | 1 cut(s) | 444 |
| MseI | TTAA | 4 cut(s) | 116, 294, 392, 526 |
| MspR9I | CCNGG | 1 cut(s) | 62 |
| Mva1269I | GAATGC | 1 cut(s) | 319 |
| MvaI | CCWGG | 1 cut(s) | 62 |
| NdeII | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| NheI | GCTAGC | 1 cut(s) | 11 |
| NlaIII | CATG | 1 cut(s) | 452 |
| PctI | GAATGC | 1 cut(s) | 319 |
| PdmI | GAANNNNTTC | 1 cut(s) | 444 |
| PfeI | GAWTC | 2 cut(s) | 70, 172 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 187 |
| PfoI | TCCNGGA | 1 cut(s) | 60 |
| PkrI | GCNGC | 1 cut(s) | 270 |
| Ple19I | CGATCG | 1 cut(s) | 127 |
| PshBI | ATTAAT | 1 cut(s) | 392 |
| Psp6I | CCWGG | 1 cut(s) | 60 |
| PspGI | CCWGG | 1 cut(s) | 60 |
| PspPI | GGNCC | 2 cut(s) | 252, 452 |
| PsuI | RGATCY | 2 cut(s) | 177, 490 |
| PvuI | CGATCG | 1 cut(s) | 127 |
| RsaI | GTAC | 1 cut(s) | 122 |
| RsaNI | GTAC | 1 cut(s) | 121 |
| Rsr2I | CGGWCCG | 1 cut(s) | 252 |
| RsrII | CGGWCCG | 1 cut(s) | 252 |
| SaqAI | TTAA | 4 cut(s) | 116, 294, 392, 526 |
| SatI | GCNGC | 1 cut(s) | 269 |
| Sau3AI | GATC | 6 cut(s) | 124, 177, 330, 485, 490, 498 |
| Sau96I | GGNCC | 2 cut(s) | 252, 452 |
| ScrFI | CCNGG | 1 cut(s) | 62 |
| SetI | ASST | 3 cut(s) | 25, 273, 461 |
| SfaNI | GCATC | 3 cut(s) | 88, 190, 316 |
| SinI | GGWCC | 1 cut(s) | 252 |
| Sse9I | AATT | 3 cut(s) | 291, 443, 474 |
| SspMI | CTAG | 2 cut(s) | 12, 38 |
| StyD4I | CCNGG | 1 cut(s) | 60 |
| StyI | CCWWGG | 2 cut(s) | 162, 204 |
| TaiI | ACGT | 2 cut(s) | 25, 461 |
| TaqI | TCGA | 2 cut(s) | 198, 237 |
| TaqII | GACCGA | 1 cut(s) | 240 |
| TasI | AATT | 3 cut(s) | 291, 443, 474 |
| TfiI | GAWTC | 2 cut(s) | 70, 172 |
| Tru1I | TTAA | 4 cut(s) | 116, 294, 392, 526 |
| Tru9I | TTAA | 4 cut(s) | 116, 294, 392, 526 |
| TseI | GCWGC | 1 cut(s) | 268 |
| TspDTI | ATGAA | 2 cut(s) | 228, 437 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 187 |
| VpaK11BI | GGWCC | 1 cut(s) | 252 |
| VspI | ATTAAT | 1 cut(s) | 392 |
| XapI | RAATTY | 1 cut(s) | 474 |
| XmnI | GAANNNNTTC | 1 cut(s) | 444 |
| XspI | CTAG | 2 cut(s) | 12, 38 |