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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 32, 44, 122, 431 |
| AdeI | CACNNNGTG | 1 cut(s) | 40 |
| AfaI | GTAC | 1 cut(s) | 196 |
| AgsI | TTSAA | 5 cut(s) | 176, 188, 374, 385, 443 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 71, 103 |
| AluBI | AGCT | 2 cut(s) | 15, 454 |
| AluI | AGCT | 2 cut(s) | 15, 454 |
| AoxI | GGCC | 1 cut(s) | 423 |
| ApeKI | GCWGC | 1 cut(s) | 12 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 183 |
| AspLEI | GCGC | 1 cut(s) | 12 |
| AsuHPI | GGTGA | 3 cut(s) | 21, 292, 420 |
| BanII | GRGCYC | 1 cut(s) | 98 |
| BauI | CACGAG | 1 cut(s) | 302 |
| BbsI | GAAGAC | 1 cut(s) | 227 |
| BbvI | GCAGC | 1 cut(s) | 24 |
| BccI | CCATC | 3 cut(s) | 76, 457, 518 |
| BfaI | CTAG | 1 cut(s) | 455 |
| BfmI | CTRYAG | 2 cut(s) | 168, 532 |
| BisI | GCNGC | 1 cut(s) | 13 |
| BlsI | GCNGC | 1 cut(s) | 14 |
| BmsI | GCATC | 1 cut(s) | 379 |
| BoxI | GACNNNNGTC | 1 cut(s) | 147 |
| BpiI | GAAGAC | 1 cut(s) | 227 |
| Bse1I | ACTGG | 1 cut(s) | 329 |
| BseGI | GGATG | 2 cut(s) | 442, 506 |
| BseNI | ACTGG | 1 cut(s) | 329 |
| BseXI | GCAGC | 1 cut(s) | 24 |
| BshFI | GGCC | 1 cut(s) | 425 |
| BsiSI | CCGG | 1 cut(s) | 434 |
| BsiWI | CGTACG | 1 cut(s) | 194 |
| BsnI | GGCC | 1 cut(s) | 425 |
| Bsp1286I | GDGCHC | 1 cut(s) | 98 |
| BspACI | CCGC | 4 cut(s) | 32, 44, 122, 431 |
| BspANI | GGCC | 1 cut(s) | 425 |
| BsrI | ACTGG | 1 cut(s) | 329 |
| BssSI | CACGAG | 1 cut(s) | 302 |
| Bst2BI | CACGAG | 1 cut(s) | 302 |
| Bst4CI | ACNGT | 3 cut(s) | 87, 103, 118 |
| Bst6I | CTCTTC | 1 cut(s) | 369 |
| BstF5I | GGATG | 2 cut(s) | 442, 506 |
| BstHHI | GCGC | 1 cut(s) | 12 |
| BstNSI | RCATGY | 1 cut(s) | 112 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 147 |
| BstSFI | CTRYAG | 2 cut(s) | 168, 532 |
| BstV1I | GCAGC | 1 cut(s) | 24 |
| BstV2I | GAAGAC | 1 cut(s) | 227 |
| BsuRI | GGCC | 1 cut(s) | 425 |
| BtsCI | GGATG | 2 cut(s) | 442, 506 |
| CfoI | GCGC | 1 cut(s) | 12 |
| CseI | GACGC | 1 cut(s) | 537 |
| Csp6I | GTAC | 1 cut(s) | 195 |
| CviAII | CATG | 1 cut(s) | 109 |
| CviJI | RGCY | 4 cut(s) | 15, 96, 425, 454 |
| CviKI_1 | RGCY | 4 cut(s) | 15, 96, 425, 454 |
| CviQI | GTAC | 1 cut(s) | 195 |
| DraIII | CACNNNGTG | 1 cut(s) | 40 |
| Eam1104I | CTCTTC | 1 cut(s) | 369 |
| EarI | CTCTTC | 1 cut(s) | 369 |
| EciI | GGCGGA | 2 cut(s) | 59, 137 |
| Eco147I | AGGCCT | 1 cut(s) | 425 |
| Eco24I | GRGCYC | 1 cut(s) | 98 |
| EcoT38I | GRGCYC | 1 cut(s) | 98 |
| FaeI | CATG | 1 cut(s) | 112 |
| FaiI | YATR | 4 cut(s) | 110, 170, 206, 354 |
| FatI | CATG | 1 cut(s) | 108 |
| Fnu4HI | GCNGC | 1 cut(s) | 13 |
| FokI | GGATG | 2 cut(s) | 449, 513 |
| FriOI | GRGCYC | 1 cut(s) | 98 |
| Fsp4HI | GCNGC | 1 cut(s) | 13 |
| FspBI | CTAG | 1 cut(s) | 455 |
| GlaI | GCGC | 1 cut(s) | 11 |
| GluI | GCNGC | 1 cut(s) | 13 |
| HaeIII | GGCC | 1 cut(s) | 425 |
| HapII | CCGG | 1 cut(s) | 434 |
| HgaI | GACGC | 1 cut(s) | 537 |
| HhaI | GCGC | 1 cut(s) | 12 |
| Hin1II | CATG | 1 cut(s) | 112 |
| Hin6I | GCGC | 1 cut(s) | 10 |
| HinP1I | GCGC | 1 cut(s) | 10 |
| HincII | GTYRAC | 1 cut(s) | 252 |
| HindII | GTYRAC | 1 cut(s) | 252 |
| HinfI | GANTC | 1 cut(s) | 80 |
| HpaI | GTTAAC | 1 cut(s) | 252 |
| HpaII | CCGG | 1 cut(s) | 434 |
| HphI | GGTGA | 3 cut(s) | 21, 292, 420 |
| Hpy166II | GTNNAC | 2 cut(s) | 106, 252 |
| Hpy188I | TCNGA | 1 cut(s) | 153 |
| Hpy188III | TCNNGA | 2 cut(s) | 91, 507 |
| Hpy8I | GTNNAC | 2 cut(s) | 106, 252 |
| HpyAV | CCTTC | 1 cut(s) | 415 |
| HpyCH4III | ACNGT | 3 cut(s) | 87, 103, 118 |
| HpyCH4IV | ACGT | 2 cut(s) | 197, 445 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 259, 543 |
| HpySE526I | ACGT | 2 cut(s) | 197, 445 |
| Hsp92II | CATG | 1 cut(s) | 112 |
| HspAI | GCGC | 1 cut(s) | 10 |
| KspAI | GTTAAC | 1 cut(s) | 252 |
| LpnPI | CCDG | 5 cut(s) | 104, 240, 310, 447, 492 |
| Lsp1109I | GCAGC | 1 cut(s) | 24 |
| LweI | GCATC | 1 cut(s) | 379 |
| MaeI | CTAG | 1 cut(s) | 455 |
| MaeII | ACGT | 2 cut(s) | 197, 445 |
| MboII | GAAGA | 6 cut(s) | 59, 188, 229, 232, 308, 386 |
| MhlI | GDGCHC | 1 cut(s) | 98 |
| MlyI | GAGTC | 1 cut(s) | 74 |
| MmeI | TCCRAC | 2 cut(s) | 131, 462 |
| MnlI | CCTC | 4 cut(s) | 37, 75, 436, 491 |
| MroXI | GAANNNNTTC | 1 cut(s) | 183 |
| MseI | TTAA | 1 cut(s) | 251 |
| MspI | CCGG | 1 cut(s) | 434 |
| NlaIII | CATG | 1 cut(s) | 112 |
| NspI | RCATGY | 1 cut(s) | 112 |
| PceI | AGGCCT | 1 cut(s) | 425 |
| PdmI | GAANNNNTTC | 1 cut(s) | 183 |
| Pfl23II | CGTACG | 1 cut(s) | 194 |
| PkrI | GCNGC | 1 cut(s) | 14 |
| PleI | GAGTC | 1 cut(s) | 74 |
| PpsI | GAGTC | 1 cut(s) | 74 |
| PshAI | GACNNNNGTC | 1 cut(s) | 147 |
| PspLI | CGTACG | 1 cut(s) | 194 |
| RsaI | GTAC | 1 cut(s) | 196 |
| RsaNI | GTAC | 1 cut(s) | 195 |
| SaqAI | TTAA | 1 cut(s) | 251 |
| SatI | GCNGC | 1 cut(s) | 13 |
| SchI | GAGTC | 1 cut(s) | 74 |
| SduI | GDGCHC | 1 cut(s) | 98 |
| SetI | ASST | 8 cut(s) | 17, 29, 42, 67, 200, 264, 448, 456 |
| SfaNI | GCATC | 1 cut(s) | 379 |
| SfcI | CTRYAG | 2 cut(s) | 168, 532 |
| SseBI | AGGCCT | 1 cut(s) | 425 |
| SsiI | CCGC | 4 cut(s) | 32, 44, 122, 431 |
| SspMI | CTAG | 1 cut(s) | 455 |
| StuI | AGGCCT | 1 cut(s) | 425 |
| TaaI | ACNGT | 3 cut(s) | 87, 103, 118 |
| TaiI | ACGT | 2 cut(s) | 200, 448 |
| TaqI | TCGA | 1 cut(s) | 59 |
| Tru1I | TTAA | 1 cut(s) | 251 |
| Tru9I | TTAA | 1 cut(s) | 251 |
| TseI | GCWGC | 1 cut(s) | 12 |
| TspDTI | ATGAA | 1 cut(s) | 434 |
| XceI | RCATGY | 1 cut(s) | 112 |
| XmnI | GAANNNNTTC | 1 cut(s) | 183 |
| XspI | CTAG | 1 cut(s) | 455 |