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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 13 |
| AciI | CCGC | 3 cut(s) | 11, 179, 183 |
| AcoI | YGGCCR | 1 cut(s) | 180 |
| AcsI | RAATTY | 3 cut(s) | 78, 352, 571 |
| AgsI | TTSAA | 3 cut(s) | 293, 418, 550 |
| AluBI | AGCT | 3 cut(s) | 71, 335, 596 |
| AluI | AGCT | 3 cut(s) | 71, 335, 596 |
| AoxI | GGCC | 2 cut(s) | 180, 391 |
| ApeKI | GCWGC | 1 cut(s) | 68 |
| ApoI | RAATTY | 3 cut(s) | 78, 352, 571 |
| AseI | ATTAAT | 1 cut(s) | 153 |
| AsuHPI | GGTGA | 3 cut(s) | 78, 255, 334 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 230, 263 |
| BanI | GGYRCC | 1 cut(s) | 13 |
| BbvI | GCAGC | 1 cut(s) | 80 |
| BfaI | CTAG | 1 cut(s) | 597 |
| BisI | GCNGC | 4 cut(s) | 12, 69, 180, 183 |
| BlsI | GCNGC | 4 cut(s) | 13, 70, 181, 184 |
| BmiI | GGNNCC | 1 cut(s) | 15 |
| BmsI | GCATC | 1 cut(s) | 115 |
| BpuEI | CTTGAG | 1 cut(s) | 119 |
| BsaJI | CCNNGG | 1 cut(s) | 31 |
| Bse1I | ACTGG | 1 cut(s) | 527 |
| BseDI | CCNNGG | 1 cut(s) | 31 |
| BseGI | GGATG | 1 cut(s) | 559 |
| BseMII | CTCAG | 2 cut(s) | 327, 393 |
| BseNI | ACTGG | 1 cut(s) | 527 |
| BseX3I | CGGCCG | 1 cut(s) | 180 |
| BseXI | GCAGC | 1 cut(s) | 80 |
| Bsh1285I | CGRYCG | 2 cut(s) | 31, 183 |
| BshFI | GGCC | 2 cut(s) | 182, 393 |
| BshNI | GGYRCC | 1 cut(s) | 13 |
| BsiEI | CGRYCG | 2 cut(s) | 31, 183 |
| BsnI | GGCC | 2 cut(s) | 182, 393 |
| Bsp143I | GATC | 4 cut(s) | 191, 252, 502, 590 |
| BspACI | CCGC | 3 cut(s) | 11, 179, 183 |
| BspANI | GGCC | 2 cut(s) | 182, 393 |
| BspCNI | CTCAG | 2 cut(s) | 328, 392 |
| BspLI | GGNNCC | 1 cut(s) | 15 |
| BspT107I | GGYRCC | 1 cut(s) | 13 |
| BsrI | ACTGG | 1 cut(s) | 527 |
| BssECI | CCNNGG | 1 cut(s) | 31 |
| BssMI | GATC | 4 cut(s) | 191, 252, 502, 590 |
| Bst6I | CTCTTC | 1 cut(s) | 413 |
| BstC8I | GCNNGC | 2 cut(s) | 130, 231 |
| BstDEI | CTNAG | 2 cut(s) | 336, 379 |
| BstF5I | GGATG | 1 cut(s) | 559 |
| BstKTI | GATC | 4 cut(s) | 194, 255, 505, 593 |
| BstMBI | GATC | 4 cut(s) | 191, 252, 502, 590 |
| BstMCI | CGRYCG | 2 cut(s) | 31, 183 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 68 |
| BstV1I | GCAGC | 1 cut(s) | 80 |
| BstZI | CGGCCG | 1 cut(s) | 180 |
| BsuRI | GGCC | 2 cut(s) | 182, 393 |
| BtgZI | GCGATG | 1 cut(s) | 495 |
| BtsCI | GGATG | 1 cut(s) | 559 |
| Cac8I | GCNNGC | 2 cut(s) | 130, 231 |
| CciNI | GCGGCCGC | 1 cut(s) | 180 |
| CviAII | CATG | 1 cut(s) | 346 |
| CviJI | RGCY | 6 cut(s) | 71, 182, 277, 335, 393, 596 |
| CviKI_1 | RGCY | 6 cut(s) | 71, 182, 277, 335, 393, 596 |
| DdeI | CTNAG | 2 cut(s) | 336, 379 |
| DpnI | GATC | 4 cut(s) | 193, 254, 504, 592 |
| DpnII | GATC | 4 cut(s) | 191, 252, 502, 590 |
| EaeI | YGGCCR | 1 cut(s) | 180 |
| EagI | CGGCCG | 1 cut(s) | 180 |
| Eam1104I | CTCTTC | 1 cut(s) | 413 |
| EarI | CTCTTC | 1 cut(s) | 413 |
| EclXI | CGGCCG | 1 cut(s) | 180 |
| Eco52I | CGGCCG | 1 cut(s) | 180 |
| EcoT22I | ATGCAT | 1 cut(s) | 160 |
| FaeI | CATG | 1 cut(s) | 349 |
| FaiI | YATR | 6 cut(s) | 227, 260, 347, 365, 405, 537 |
| FatI | CATG | 1 cut(s) | 345 |
| Fnu4HI | GCNGC | 4 cut(s) | 12, 69, 180, 183 |
| FokI | GGATG | 1 cut(s) | 566 |
| Fsp4HI | GCNGC | 4 cut(s) | 12, 69, 180, 183 |
| FspBI | CTAG | 1 cut(s) | 597 |
| GluI | GCNGC | 4 cut(s) | 12, 69, 180, 183 |
| HaeIII | GGCC | 2 cut(s) | 182, 393 |
| Hin1II | CATG | 1 cut(s) | 349 |
| HinfI | GANTC | 2 cut(s) | 377, 383 |
| HphI | GGTGA | 3 cut(s) | 78, 255, 334 |
| Hpy166II | GTNNAC | 1 cut(s) | 342 |
| Hpy188I | TCNGA | 2 cut(s) | 252, 382 |
| Hpy188III | TCNNGA | 1 cut(s) | 98 |
| Hpy8I | GTNNAC | 1 cut(s) | 342 |
| HpyAV | CCTTC | 1 cut(s) | 544 |
| HpyCH4V | TGCA | 4 cut(s) | 132, 158, 229, 562 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 68 |
| HpyF3I | CTNAG | 2 cut(s) | 336, 379 |
| Hsp92II | CATG | 1 cut(s) | 349 |
| Kzo9I | GATC | 4 cut(s) | 191, 252, 502, 590 |
| LpnPI | CCDG | 4 cut(s) | 279, 354, 359, 508 |
| Lsp1109I | GCAGC | 1 cut(s) | 80 |
| LweI | GCATC | 1 cut(s) | 115 |
| MaeI | CTAG | 1 cut(s) | 597 |
| MaeIII | GTNAC | 1 cut(s) | 42 |
| MalI | GATC | 4 cut(s) | 193, 254, 504, 592 |
| MboI | GATC | 4 cut(s) | 191, 252, 502, 590 |
| MboII | GAAGA | 5 cut(s) | 134, 371, 430, 600, 612 |
| MluCI | AATT | 5 cut(s) | 78, 107, 301, 352, 571 |
| MlyI | GAGTC | 1 cut(s) | 371 |
| MmeI | TCCRAC | 1 cut(s) | 440 |
| Mph1103I | ATGCAT | 1 cut(s) | 160 |
| MseI | TTAA | 3 cut(s) | 51, 105, 153 |
| MslI | CAYNNNNRTG | 1 cut(s) | 52 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 68 |
| NdeII | GATC | 4 cut(s) | 191, 252, 502, 590 |
| NlaIII | CATG | 1 cut(s) | 349 |
| NlaIV | GGNNCC | 1 cut(s) | 15 |
| NotI | GCGGCCGC | 1 cut(s) | 180 |
| NsiI | ATGCAT | 1 cut(s) | 160 |
| PfeI | GAWTC | 1 cut(s) | 383 |
| PkrI | GCNGC | 4 cut(s) | 13, 70, 181, 184 |
| PleI | GAGTC | 1 cut(s) | 371 |
| PpsI | GAGTC | 1 cut(s) | 371 |
| PshBI | ATTAAT | 1 cut(s) | 153 |
| PspN4I | GGNNCC | 1 cut(s) | 15 |
| RseI | CAYNNNNRTG | 1 cut(s) | 52 |
| SaqAI | TTAA | 3 cut(s) | 51, 105, 153 |
| SatI | GCNGC | 4 cut(s) | 12, 69, 180, 183 |
| Sau3AI | GATC | 4 cut(s) | 191, 252, 502, 590 |
| SchI | GAGTC | 1 cut(s) | 371 |
| SfaNI | GCATC | 1 cut(s) | 115 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 52 |
| SmlI | CTYRAG | 1 cut(s) | 98 |
| SmoI | CTYRAG | 1 cut(s) | 98 |
| Sse9I | AATT | 5 cut(s) | 78, 107, 301, 352, 571 |
| SsiI | CCGC | 3 cut(s) | 11, 179, 183 |
| SspMI | CTAG | 1 cut(s) | 597 |
| TaqII | GACCGA | 1 cut(s) | 45 |
| TasI | AATT | 5 cut(s) | 78, 107, 301, 352, 571 |
| TauI | GCSGC | 3 cut(s) | 14, 182, 185 |
| TfiI | GAWTC | 1 cut(s) | 383 |
| Tru1I | TTAA | 3 cut(s) | 51, 105, 153 |
| Tru9I | TTAA | 3 cut(s) | 51, 105, 153 |
| TseI | GCWGC | 1 cut(s) | 68 |
| TspDTI | ATGAA | 3 cut(s) | 71, 484, 570 |
| TspGWI | ACGGA | 1 cut(s) | 332 |
| VspI | ATTAAT | 1 cut(s) | 153 |
| XapI | RAATTY | 3 cut(s) | 78, 352, 571 |
| XspI | CTAG | 1 cut(s) | 597 |
| Zsp2I | ATGCAT | 1 cut(s) | 160 |