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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 565 |
| Acc36I | ACCTGC | 1 cut(s) | 1018 |
| AccI | GTMKAC | 1 cut(s) | 1044 |
| AcsI | RAATTY | 1 cut(s) | 334 |
| AcuI | CTGAAG | 3 cut(s) | 23, 144, 173 |
| AfaI | GTAC | 2 cut(s) | 694, 880 |
| AgsI | TTSAA | 6 cut(s) | 241, 379, 674, 686, 806, 817 |
| AjnI | CCWGG | 1 cut(s) | 1059 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 569, 601 |
| AluBI | AGCT | 8 cut(s) | 92, 158, 187, 428, 441, 488, 638, 1006 |
| AluI | AGCT | 8 cut(s) | 92, 158, 187, 428, 441, 488, 638, 1006 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 872 |
| AoxI | GGCC | 2 cut(s) | 400, 855 |
| ApaI | GGGCCC | 1 cut(s) | 404 |
| ApeKI | GCWGC | 5 cut(s) | 425, 433, 488, 500, 962 |
| ApoI | RAATTY | 1 cut(s) | 334 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 681 |
| AspS9I | GGNCC | 3 cut(s) | 400, 401, 1057 |
| AsuHPI | GGTGA | 3 cut(s) | 307, 367, 791 |
| AvaII | GGWCC | 1 cut(s) | 1057 |
| BaeGI | GKGCMC | 1 cut(s) | 404 |
| BanII | GRGCYC | 3 cut(s) | 404, 596, 1081 |
| BbsI | GAAGAC | 1 cut(s) | 725 |
| BbvI | GCAGC | 5 cut(s) | 437, 445, 475, 487, 949 |
| BccI | CCATC | 3 cut(s) | 889, 949, 1111 |
| BciT130I | CCWGG | 1 cut(s) | 1061 |
| BclI | TGATCA | 1 cut(s) | 97 |
| BfaI | CTAG | 2 cut(s) | 128, 887 |
| BfmI | CTRYAG | 3 cut(s) | 222, 666, 963 |
| BfuAI | ACCTGC | 1 cut(s) | 1018 |
| BisI | GCNGC | 5 cut(s) | 426, 434, 489, 501, 963 |
| BlpI | GCTNAGC | 1 cut(s) | 429 |
| BlsI | GCNGC | 5 cut(s) | 427, 435, 490, 502, 964 |
| Bme1390I | CCNGG | 1 cut(s) | 1061 |
| Bme18I | GGWCC | 1 cut(s) | 1057 |
| BmgT120I | GGNCC | 3 cut(s) | 400, 401, 1057 |
| BmiI | GGNNCC | 1 cut(s) | 402 |
| BmrFI | CCNGG | 1 cut(s) | 1061 |
| BmrI | ACTGGG | 1 cut(s) | 1036 |
| BmsI | GCATC | 4 cut(s) | 356, 500, 811, 859 |
| BmuI | ACTGGG | 1 cut(s) | 1036 |
| BoxI | GACNNNNGTC | 1 cut(s) | 645 |
| BpiI | GAAGAC | 1 cut(s) | 725 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 429 |
| BsaWI | WCCGGW | 1 cut(s) | 776 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 380, 410, 569, 599 |
| Bse1I | ACTGG | 2 cut(s) | 1031, 1094 |
| BseBI | CCWGG | 1 cut(s) | 1061 |
| BseGI | GGATG | 1 cut(s) | 937 |
| BseMII | CTCAG | 1 cut(s) | 443 |
| BseNI | ACTGG | 2 cut(s) | 1031, 1094 |
| BseRI | GAGGAG | 5 cut(s) | 53, 215, 218, 1046, 1087 |
| BseSI | GKGCMC | 1 cut(s) | 404 |
| BseXI | GCAGC | 5 cut(s) | 437, 445, 475, 487, 949 |
| BshFI | GGCC | 2 cut(s) | 402, 857 |
| BsiSI | CCGG | 1 cut(s) | 777 |
| BsiWI | CGTACG | 1 cut(s) | 692 |
| BsnI | GGCC | 2 cut(s) | 402, 857 |
| Bsp120I | GGGCCC | 1 cut(s) | 400 |
| Bsp1286I | GDGCHC | 3 cut(s) | 404, 596, 1081 |
| Bsp143I | GATC | 3 cut(s) | 47, 97, 523 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 429 |
| BspANI | GGCC | 2 cut(s) | 402, 857 |
| BspCNI | CTCAG | 1 cut(s) | 442 |
| BspLI | GGNNCC | 1 cut(s) | 402 |
| BspMAI | CTGCAG | 2 cut(s) | 226, 967 |
| BspMI | ACCTGC | 1 cut(s) | 1018 |
| BsrI | ACTGG | 2 cut(s) | 1031, 1094 |
| BssMI | GATC | 3 cut(s) | 47, 97, 523 |
| BssNAI | GTATAC | 1 cut(s) | 1045 |
| Bst1107I | GTATAC | 1 cut(s) | 1045 |
| Bst2UI | CCWGG | 1 cut(s) | 1061 |
| Bst4CI | ACNGT | 5 cut(s) | 67, 535, 585, 601, 616 |
| Bst6I | CTCTTC | 3 cut(s) | 30, 801, 990 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 971 |
| BstDEI | CTNAG | 4 cut(s) | 272, 429, 495, 1021 |
| BstF5I | GGATG | 1 cut(s) | 937 |
| BstKTI | GATC | 3 cut(s) | 50, 100, 526 |
| BstMBI | GATC | 3 cut(s) | 47, 97, 523 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 497, 971 |
| BstNI | CCWGG | 1 cut(s) | 1061 |
| BstNSI | RCATGY | 1 cut(s) | 610 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 645 |
| BstSCI | CCNGG | 1 cut(s) | 1059 |
| BstSFI | CTRYAG | 3 cut(s) | 222, 666, 963 |
| BstSLI | GKGCMC | 1 cut(s) | 404 |
| BstV1I | GCAGC | 5 cut(s) | 437, 445, 475, 487, 949 |
| BstV2I | GAAGAC | 1 cut(s) | 725 |
| BstZ17I | GTATAC | 1 cut(s) | 1045 |
| BsuRI | GGCC | 2 cut(s) | 402, 857 |
| BtsCI | GGATG | 1 cut(s) | 937 |
| BtsI | GCAGTG | 1 cut(s) | 860 |
| BtsIMutI | CAGTG | 2 cut(s) | 621, 860 |
| BveI | ACCTGC | 1 cut(s) | 1018 |
| CaiI | CAGNNNCTG | 1 cut(s) | 872 |
| Cfr13I | GGNCC | 3 cut(s) | 400, 401, 1057 |
| CseI | GACGC | 1 cut(s) | 968 |
| Csp6I | GTAC | 2 cut(s) | 693, 879 |
| CviAII | CATG | 2 cut(s) | 292, 607 |
| CviQI | GTAC | 2 cut(s) | 693, 879 |
| DdeI | CTNAG | 4 cut(s) | 272, 429, 495, 1021 |
| DpnI | GATC | 3 cut(s) | 49, 99, 525 |
| DpnII | GATC | 3 cut(s) | 47, 97, 523 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 565 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 565 |
| Eam1104I | CTCTTC | 3 cut(s) | 30, 801, 990 |
| EarI | CTCTTC | 3 cut(s) | 30, 801, 990 |
| Eco147I | AGGCCT | 1 cut(s) | 857 |
| Eco24I | GRGCYC | 3 cut(s) | 404, 596, 1081 |
| Eco47I | GGWCC | 1 cut(s) | 1057 |
| Eco57I | CTGAAG | 3 cut(s) | 23, 144, 173 |
| EcoRII | CCWGG | 1 cut(s) | 1059 |
| EcoT38I | GRGCYC | 3 cut(s) | 404, 596, 1081 |
| FaeI | CATG | 2 cut(s) | 295, 610 |
| FatI | CATG | 2 cut(s) | 291, 606 |
| FbaI | TGATCA | 1 cut(s) | 97 |
| FblI | GTMKAC | 1 cut(s) | 1044 |
| Fnu4HI | GCNGC | 5 cut(s) | 426, 434, 489, 501, 963 |
| FokI | GGATG | 1 cut(s) | 944 |
| FriOI | GRGCYC | 3 cut(s) | 404, 596, 1081 |
| Fsp4HI | GCNGC | 5 cut(s) | 426, 434, 489, 501, 963 |
| FspBI | CTAG | 2 cut(s) | 128, 887 |
| GluI | GCNGC | 5 cut(s) | 426, 434, 489, 501, 963 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HaeIII | GGCC | 2 cut(s) | 402, 857 |
| HapII | CCGG | 1 cut(s) | 777 |
| HgaI | GACGC | 1 cut(s) | 968 |
| Hin1II | CATG | 2 cut(s) | 295, 610 |
| HincII | GTYRAC | 1 cut(s) | 750 |
| HindII | GTYRAC | 1 cut(s) | 750 |
| HinfI | GANTC | 6 cut(s) | 20, 26, 298, 409, 578, 733 |
| HpaI | GTTAAC | 1 cut(s) | 750 |
| HpaII | CCGG | 1 cut(s) | 777 |
| HphI | GGTGA | 3 cut(s) | 307, 367, 791 |
| Hpy166II | GTNNAC | 3 cut(s) | 604, 750, 1045 |
| Hpy188I | TCNGA | 7 cut(s) | 25, 31, 52, 102, 163, 192, 651 |
| Hpy188III | TCNNGA | 3 cut(s) | 71, 302, 589 |
| Hpy8I | GTNNAC | 3 cut(s) | 604, 750, 1045 |
| Hpy99I | CGWCG | 1 cut(s) | 355 |
| HpyAV | CCTTC | 4 cut(s) | 68, 352, 458, 847 |
| HpyCH4III | ACNGT | 5 cut(s) | 67, 535, 585, 601, 616 |
| HpyCH4IV | ACGT | 2 cut(s) | 695, 877 |
| HpyCH4V | TGCA | 7 cut(s) | 224, 347, 491, 757, 965, 974, 1100 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 497, 971 |
| HpyF3I | CTNAG | 4 cut(s) | 272, 429, 495, 1021 |
| HpySE526I | ACGT | 2 cut(s) | 695, 877 |
| Hsp92II | CATG | 2 cut(s) | 295, 610 |
| Ksp22I | TGATCA | 1 cut(s) | 97 |
| KspAI | GTTAAC | 1 cut(s) | 750 |
| Kzo9I | GATC | 3 cut(s) | 47, 97, 523 |
| LmnI | GCTCC | 3 cut(s) | 89, 388, 446 |
| Lsp1109I | GCAGC | 5 cut(s) | 437, 445, 475, 487, 949 |
| LweI | GCATC | 4 cut(s) | 356, 500, 811, 859 |
| MaeI | CTAG | 2 cut(s) | 128, 887 |
| MaeII | ACGT | 2 cut(s) | 695, 877 |
| MaeIII | GTNAC | 3 cut(s) | 325, 567, 579 |
| MalI | GATC | 3 cut(s) | 49, 99, 525 |
| MboI | GATC | 3 cut(s) | 47, 97, 523 |
| MhlI | GDGCHC | 3 cut(s) | 404, 596, 1081 |
| MluCI | AATT | 7 cut(s) | 103, 235, 334, 374, 476, 911, 1048 |
| MlyI | GAGTC | 3 cut(s) | 403, 587, 742 |
| MroXI | GAANNNNTTC | 1 cut(s) | 681 |
| MseI | TTAA | 2 cut(s) | 479, 749 |
| MslI | CAYNNNNRTG | 1 cut(s) | 777 |
| MspI | CCGG | 1 cut(s) | 777 |
| MspR9I | CCNGG | 1 cut(s) | 1061 |
| MvaI | CCWGG | 1 cut(s) | 1061 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 497, 971 |
| NdeII | GATC | 3 cut(s) | 47, 97, 523 |
| NlaIII | CATG | 2 cut(s) | 295, 610 |
| NlaIV | GGNNCC | 1 cut(s) | 402 |
| NmuCI | GTSAC | 3 cut(s) | 325, 567, 579 |
| NspI | RCATGY | 1 cut(s) | 610 |
| PceI | AGGCCT | 1 cut(s) | 857 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 563 |
| PdmI | GAANNNNTTC | 1 cut(s) | 681 |
| PfeI | GAWTC | 3 cut(s) | 20, 26, 298 |
| Pfl23II | CGTACG | 1 cut(s) | 692 |
| PkrI | GCNGC | 5 cut(s) | 427, 435, 490, 502, 964 |
| PleI | GAGTC | 3 cut(s) | 403, 586, 741 |
| PpsI | GAGTC | 3 cut(s) | 403, 586, 741 |
| PshAI | GACNNNNGTC | 1 cut(s) | 645 |
| Psp6I | CCWGG | 1 cut(s) | 1059 |
| PspGI | CCWGG | 1 cut(s) | 1059 |
| PspLI | CGTACG | 1 cut(s) | 692 |
| PspN4I | GGNNCC | 1 cut(s) | 402 |
| PspOMI | GGGCCC | 1 cut(s) | 400 |
| PspPI | GGNCC | 3 cut(s) | 400, 401, 1057 |
| PstI | CTGCAG | 2 cut(s) | 226, 967 |
| PstNI | CAGNNNCTG | 1 cut(s) | 872 |
| RsaI | GTAC | 2 cut(s) | 694, 880 |
| RsaNI | GTAC | 2 cut(s) | 693, 879 |
| RseI | CAYNNNNRTG | 1 cut(s) | 777 |
| SaqAI | TTAA | 2 cut(s) | 479, 749 |
| SatI | GCNGC | 5 cut(s) | 426, 434, 489, 501, 963 |
| Sau3AI | GATC | 3 cut(s) | 47, 97, 523 |
| Sau96I | GGNCC | 3 cut(s) | 400, 401, 1057 |
| SchI | GAGTC | 3 cut(s) | 403, 587, 742 |
| ScrFI | CCNGG | 1 cut(s) | 1061 |
| SduI | GDGCHC | 3 cut(s) | 404, 596, 1081 |
| SfaNI | GCATC | 4 cut(s) | 356, 500, 811, 859 |
| SfcI | CTRYAG | 3 cut(s) | 222, 666, 963 |
| SinI | GGWCC | 1 cut(s) | 1057 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 777 |
| Sse9I | AATT | 7 cut(s) | 103, 235, 334, 374, 476, 911, 1048 |
| SseBI | AGGCCT | 1 cut(s) | 857 |
| SspMI | CTAG | 2 cut(s) | 128, 887 |
| StuI | AGGCCT | 1 cut(s) | 857 |
| StyD4I | CCNGG | 1 cut(s) | 1059 |
| TaaI | ACNGT | 5 cut(s) | 67, 535, 585, 601, 616 |
| TaiI | ACGT | 2 cut(s) | 698, 880 |
| TaqI | TCGA | 2 cut(s) | 530, 557 |
| TaqII | GACCGA | 1 cut(s) | 344 |
| TasI | AATT | 7 cut(s) | 103, 235, 334, 374, 476, 911, 1048 |
| TfiI | GAWTC | 3 cut(s) | 20, 26, 298 |
| Tru1I | TTAA | 2 cut(s) | 479, 749 |
| Tru9I | TTAA | 2 cut(s) | 479, 749 |
| TscAI | CASTG | 2 cut(s) | 621, 867 |
| TseFI | GTSAC | 3 cut(s) | 325, 567, 579 |
| TseI | GCWGC | 5 cut(s) | 425, 433, 488, 500, 962 |
| Tsp45I | GTSAC | 3 cut(s) | 325, 567, 579 |
| TspDTI | ATGAA | 6 cut(s) | 69, 129, 396, 489, 498, 866 |
| TspGWI | ACGGA | 1 cut(s) | 555 |
| TspRI | CASTG | 2 cut(s) | 621, 867 |
| VpaK11BI | GGWCC | 1 cut(s) | 1057 |
| XapI | RAATTY | 1 cut(s) | 334 |
| XceI | RCATGY | 1 cut(s) | 610 |
| XmiI | GTMKAC | 1 cut(s) | 1044 |
| XmnI | GAANNNNTTC | 1 cut(s) | 681 |
| XspI | CTAG | 2 cut(s) | 128, 887 |