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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1708 |
| AccI | GTMKAC | 1 cut(s) | 1734 |
| AccIII | TCCGGA | 1 cut(s) | 655 |
| AcsI | RAATTY | 1 cut(s) | 919 |
| AcuI | CTGAAG | 3 cut(s) | 608, 729, 758 |
| AfaI | GTAC | 1 cut(s) | 1791 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 592 |
| AflIII | ACRYGT | 1 cut(s) | 58 |
| AgsI | TTSAA | 7 cut(s) | 581, 826, 964, 1256, 1268, 1496, 1507 |
| AjnI | CCWGG | 1 cut(s) | 1749 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 9, 41 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1151, 1183 |
| AluBI | AGCT | 8 cut(s) | 677, 743, 772, 835, 1013, 1026, 1073, 1220 |
| AluI | AGCT | 8 cut(s) | 677, 743, 772, 835, 1013, 1026, 1073, 1220 |
| Alw26I | GTCTC | 1 cut(s) | 1388 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1562 |
| Aor13HI | TCCGGA | 1 cut(s) | 655 |
| ApeKI | GCWGC | 6 cut(s) | 932, 1010, 1018, 1073, 1085, 1652 |
| ApoI | RAATTY | 1 cut(s) | 919 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1263 |
| AspS9I | GGNCC | 1 cut(s) | 1747 |
| AsuHPI | GGTGA | 3 cut(s) | 892, 952, 1373 |
| AvaII | GGWCC | 1 cut(s) | 1747 |
| BanII | GRGCYC | 2 cut(s) | 989, 1178 |
| BauI | CACGAG | 1 cut(s) | 69 |
| BbsI | GAAGAC | 1 cut(s) | 1307 |
| BbvCI | CCTCAGC | 1 cut(s) | 1704 |
| BbvI | GCAGC | 6 cut(s) | 944, 1022, 1030, 1060, 1072, 1639 |
| BccI | CCATC | 4 cut(s) | 1156, 1579, 1639, 1801 |
| BciT130I | CCWGG | 1 cut(s) | 1751 |
| BclI | TGATCA | 1 cut(s) | 682 |
| BcoDI | GTCTC | 1 cut(s) | 1388 |
| BfaI | CTAG | 2 cut(s) | 713, 1577 |
| BfmI | CTRYAG | 3 cut(s) | 807, 1248, 1653 |
| BfuAI | ACCTGC | 1 cut(s) | 1708 |
| BisI | GCNGC | 6 cut(s) | 933, 1011, 1019, 1074, 1086, 1653 |
| BlpI | GCTNAGC | 1 cut(s) | 1014 |
| BlsI | GCNGC | 6 cut(s) | 934, 1012, 1020, 1075, 1087, 1654 |
| Bme1390I | CCNGG | 1 cut(s) | 1751 |
| Bme18I | GGWCC | 1 cut(s) | 1747 |
| BmgT120I | GGNCC | 1 cut(s) | 1747 |
| BmrFI | CCNGG | 1 cut(s) | 1751 |
| BmrI | ACTGGG | 1 cut(s) | 1726 |
| BmsI | GCATC | 3 cut(s) | 1085, 1501, 1549 |
| BmuI | ACTGGG | 1 cut(s) | 1726 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1227 |
| BpiI | GAAGAC | 1 cut(s) | 1307 |
| BpmI | CTGGAG | 1 cut(s) | 908 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1704 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1014 |
| BsaI | GGTCTC | 1 cut(s) | 1388 |
| BsaWI | WCCGGW | 1 cut(s) | 655 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 965, 995, 1151, 1181 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 592 |
| Bse3DI | GCAATG | 1 cut(s) | 63 |
| BseAI | TCCGGA | 1 cut(s) | 655 |
| BseBI | CCWGG | 1 cut(s) | 1751 |
| BseGI | GGATG | 1 cut(s) | 1627 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 592 |
| BseMI | GCAATG | 1 cut(s) | 63 |
| BseMII | CTCAG | 2 cut(s) | 1028, 1695 |
| BseRI | GAGGAG | 5 cut(s) | 638, 800, 803, 1736, 1777 |
| BseXI | GCAGC | 6 cut(s) | 944, 1022, 1030, 1060, 1072, 1639 |
| BsiSI | CCGG | 1 cut(s) | 656 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 592 |
| BsmAI | GTCTC | 1 cut(s) | 1388 |
| Bso31I | GGTCTC | 1 cut(s) | 1388 |
| Bsp1286I | GDGCHC | 2 cut(s) | 989, 1178 |
| Bsp13I | TCCGGA | 1 cut(s) | 655 |
| Bsp143I | GATC | 3 cut(s) | 632, 682, 1108 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1014 |
| BspCNI | CTCAG | 2 cut(s) | 1027, 1696 |
| BspEI | TCCGGA | 1 cut(s) | 655 |
| BspMAI | CTGCAG | 2 cut(s) | 811, 1657 |
| BspMI | ACCTGC | 1 cut(s) | 1708 |
| BspTNI | GGTCTC | 1 cut(s) | 1388 |
| BsrDI | GCAATG | 1 cut(s) | 63 |
| BssMI | GATC | 3 cut(s) | 632, 682, 1108 |
| BssNAI | GTATAC | 1 cut(s) | 1735 |
| BssSI | CACGAG | 1 cut(s) | 69 |
| Bst1107I | GTATAC | 1 cut(s) | 1735 |
| Bst2BI | CACGAG | 1 cut(s) | 69 |
| Bst2UI | CCWGG | 1 cut(s) | 1751 |
| Bst6I | CTCTTC | 4 cut(s) | 615, 843, 1491, 1680 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1661 |
| BstDEI | CTNAG | 4 cut(s) | 857, 1014, 1080, 1704 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 590 |
| BstF5I | GGATG | 1 cut(s) | 1627 |
| BstKTI | GATC | 3 cut(s) | 635, 685, 1111 |
| BstMAI | GTCTC | 1 cut(s) | 1388 |
| BstMBI | GATC | 3 cut(s) | 632, 682, 1108 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1082, 1661 |
| BstNI | CCWGG | 1 cut(s) | 1751 |
| BstNSI | RCATGY | 3 cut(s) | 8, 62, 1192 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1227 |
| BstSCI | CCNGG | 1 cut(s) | 1749 |
| BstSFI | CTRYAG | 3 cut(s) | 807, 1248, 1653 |
| BstV1I | GCAGC | 6 cut(s) | 944, 1022, 1030, 1060, 1072, 1639 |
| BstV2I | GAAGAC | 1 cut(s) | 1307 |
| BstZ17I | GTATAC | 1 cut(s) | 1735 |
| BtsCI | GGATG | 1 cut(s) | 1627 |
| BveI | ACCTGC | 1 cut(s) | 1708 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1562 |
| Cfr13I | GGNCC | 1 cut(s) | 1747 |
| CseI | GACGC | 2 cut(s) | 924, 1658 |
| Csp6I | GTAC | 1 cut(s) | 1790 |
| CviQI | GTAC | 1 cut(s) | 1790 |
| DdeI | CTNAG | 4 cut(s) | 857, 1014, 1080, 1704 |
| DpnI | GATC | 3 cut(s) | 634, 684, 1110 |
| DpnII | GATC | 3 cut(s) | 632, 682, 1108 |
| Eam1104I | CTCTTC | 4 cut(s) | 615, 843, 1491, 1680 |
| EarI | CTCTTC | 4 cut(s) | 615, 843, 1491, 1680 |
| Eco147I | AGGCCT | 1 cut(s) | 1547 |
| Eco24I | GRGCYC | 2 cut(s) | 989, 1178 |
| Eco31I | GGTCTC | 1 cut(s) | 1388 |
| Eco32I | GATATC | 1 cut(s) | 1371 |
| Eco47I | GGWCC | 1 cut(s) | 1747 |
| Eco57I | CTGAAG | 3 cut(s) | 608, 729, 758 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 590 |
| EcoRII | CCWGG | 1 cut(s) | 1749 |
| EcoRV | GATATC | 1 cut(s) | 1371 |
| EcoT38I | GRGCYC | 2 cut(s) | 989, 1178 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 9, 41 |
| FbaI | TGATCA | 1 cut(s) | 682 |
| FblI | GTMKAC | 1 cut(s) | 1734 |
| Fnu4HI | GCNGC | 6 cut(s) | 933, 1011, 1019, 1074, 1086, 1653 |
| FokI | GGATG | 1 cut(s) | 1634 |
| FriOI | GRGCYC | 2 cut(s) | 989, 1178 |
| Fsp4HI | GCNGC | 6 cut(s) | 933, 1011, 1019, 1074, 1086, 1653 |
| FspBI | CTAG | 2 cut(s) | 713, 1577 |
| GluI | GCNGC | 6 cut(s) | 933, 1011, 1019, 1074, 1086, 1653 |
| GsuI | CTGGAG | 1 cut(s) | 908 |
| HapII | CCGG | 1 cut(s) | 656 |
| HgaI | GACGC | 2 cut(s) | 924, 1658 |
| HincII | GTYRAC | 1 cut(s) | 1332 |
| HindII | GTYRAC | 1 cut(s) | 1332 |
| HinfI | GANTC | 6 cut(s) | 605, 611, 883, 994, 1160, 1767 |
| HpaI | GTTAAC | 1 cut(s) | 1332 |
| HpaII | CCGG | 1 cut(s) | 656 |
| HphI | GGTGA | 3 cut(s) | 892, 952, 1373 |
| Hpy166II | GTNNAC | 5 cut(s) | 56, 1186, 1332, 1714, 1735 |
| Hpy188I | TCNGA | 6 cut(s) | 610, 616, 637, 687, 777, 1233 |
| Hpy188III | TCNNGA | 7 cut(s) | 656, 747, 887, 1171, 1556, 1628, 1771 |
| Hpy8I | GTNNAC | 5 cut(s) | 56, 1186, 1332, 1714, 1735 |
| Hpy99I | CGWCG | 1 cut(s) | 940 |
| HpyAV | CCTTC | 7 cut(s) | 50, 583, 653, 937, 958, 1043, 1537 |
| HpyCH4IV | ACGT | 3 cut(s) | 1395, 1567, 1731 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 809, 932, 1076, 1339, 1655, 1664 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1082, 1661 |
| HpyF3I | CTNAG | 4 cut(s) | 857, 1014, 1080, 1704 |
| HpySE526I | ACGT | 3 cut(s) | 1395, 1567, 1731 |
| Kpn2I | TCCGGA | 1 cut(s) | 655 |
| Ksp22I | TGATCA | 1 cut(s) | 682 |
| KspAI | GTTAAC | 1 cut(s) | 1332 |
| Kzo9I | GATC | 3 cut(s) | 632, 682, 1108 |
| LmnI | GCTCC | 3 cut(s) | 674, 973, 1031 |
| Lsp1109I | GCAGC | 6 cut(s) | 944, 1022, 1030, 1060, 1072, 1639 |
| LweI | GCATC | 3 cut(s) | 1085, 1501, 1549 |
| MaeI | CTAG | 2 cut(s) | 713, 1577 |
| MaeII | ACGT | 3 cut(s) | 1395, 1567, 1731 |
| MalI | GATC | 3 cut(s) | 634, 684, 1110 |
| MboI | GATC | 3 cut(s) | 632, 682, 1108 |
| MhlI | GDGCHC | 2 cut(s) | 989, 1178 |
| MluCI | AATT | 7 cut(s) | 688, 820, 919, 959, 1061, 1601, 1738 |
| MlyI | GAGTC | 3 cut(s) | 988, 1169, 1776 |
| MroI | TCCGGA | 1 cut(s) | 655 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1263 |
| MseI | TTAA | 3 cut(s) | 1064, 1331, 1406 |
| MslI | CAYNNNNRTG | 2 cut(s) | 9, 1359 |
| MspI | CCGG | 1 cut(s) | 656 |
| MspR9I | CCNGG | 1 cut(s) | 1751 |
| MvaI | CCWGG | 1 cut(s) | 1751 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1082, 1661 |
| NdeII | GATC | 3 cut(s) | 632, 682, 1108 |
| NspI | RCATGY | 3 cut(s) | 8, 62, 1192 |
| PceI | AGGCCT | 1 cut(s) | 1547 |
| PciI | ACATGT | 1 cut(s) | 58 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1263 |
| PfeI | GAWTC | 3 cut(s) | 605, 611, 883 |
| PkrI | GCNGC | 6 cut(s) | 934, 1012, 1020, 1075, 1087, 1654 |
| PleI | GAGTC | 3 cut(s) | 988, 1168, 1775 |
| PpsI | GAGTC | 3 cut(s) | 988, 1168, 1775 |
| PscI | ACATGT | 1 cut(s) | 58 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1227 |
| Psp6I | CCWGG | 1 cut(s) | 1749 |
| PspGI | CCWGG | 1 cut(s) | 1749 |
| PspPI | GGNCC | 1 cut(s) | 1747 |
| PstI | CTGCAG | 2 cut(s) | 811, 1657 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1562 |
| RsaI | GTAC | 1 cut(s) | 1791 |
| RsaNI | GTAC | 1 cut(s) | 1790 |
| RseI | CAYNNNNRTG | 2 cut(s) | 9, 1359 |
| SaqAI | TTAA | 3 cut(s) | 1064, 1331, 1406 |
| SatI | GCNGC | 6 cut(s) | 933, 1011, 1019, 1074, 1086, 1653 |
| Sau3AI | GATC | 3 cut(s) | 632, 682, 1108 |
| Sau96I | GGNCC | 1 cut(s) | 1747 |
| SchI | GAGTC | 3 cut(s) | 988, 1169, 1776 |
| ScrFI | CCNGG | 1 cut(s) | 1751 |
| SduI | GDGCHC | 2 cut(s) | 989, 1178 |
| SfaNI | GCATC | 3 cut(s) | 1085, 1501, 1549 |
| SfcI | CTRYAG | 3 cut(s) | 807, 1248, 1653 |
| SinI | GGWCC | 1 cut(s) | 1747 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 9, 1359 |
| Sse9I | AATT | 7 cut(s) | 688, 820, 919, 959, 1061, 1601, 1738 |
| SseBI | AGGCCT | 1 cut(s) | 1547 |
| SspMI | CTAG | 2 cut(s) | 713, 1577 |
| StuI | AGGCCT | 1 cut(s) | 1547 |
| StyD4I | CCNGG | 1 cut(s) | 1749 |
| TaiI | ACGT | 3 cut(s) | 1398, 1570, 1734 |
| TaqI | TCGA | 1 cut(s) | 1139 |
| TaqII | GACCGA | 1 cut(s) | 929 |
| TasI | AATT | 7 cut(s) | 688, 820, 919, 959, 1061, 1601, 1738 |
| TfiI | GAWTC | 3 cut(s) | 605, 611, 883 |
| Tru1I | TTAA | 3 cut(s) | 1064, 1331, 1406 |
| Tru9I | TTAA | 3 cut(s) | 1064, 1331, 1406 |
| TseI | GCWGC | 6 cut(s) | 932, 1010, 1018, 1073, 1085, 1652 |
| TspDTI | ATGAA | 7 cut(s) | 17, 32, 654, 855, 1074, 1083, 1556 |
| VpaK11BI | GGWCC | 1 cut(s) | 1747 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 590 |
| XapI | RAATTY | 1 cut(s) | 919 |
| XceI | RCATGY | 3 cut(s) | 8, 62, 1192 |
| XmiI | GTMKAC | 1 cut(s) | 1734 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1263 |
| XspI | CTAG | 2 cut(s) | 713, 1577 |