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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 481 |
| AclWI | GGATC | 4 cut(s) | 188, 199, 429, 466 |
| AcsI | RAATTY | 3 cut(s) | 173, 269, 514 |
| AcuI | CTGAAG | 1 cut(s) | 434 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 127, 224 |
| AgsI | TTSAA | 2 cut(s) | 201, 407 |
| AjnI | CCWGG | 1 cut(s) | 7 |
| AluBI | AGCT | 4 cut(s) | 317, 329, 412, 479 |
| AluI | AGCT | 4 cut(s) | 317, 329, 412, 479 |
| AlwI | GGATC | 4 cut(s) | 188, 199, 429, 466 |
| Aor13HI | TCCGGA | 1 cut(s) | 481 |
| ApoI | RAATTY | 3 cut(s) | 173, 269, 514 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 173 |
| AspA2I | CCTAGG | 1 cut(s) | 208 |
| AspS9I | GGNCC | 1 cut(s) | 235 |
| AsuHPI | GGTGA | 2 cut(s) | 80, 239 |
| AvaII | GGWCC | 1 cut(s) | 235 |
| AvrII | CCTAGG | 1 cut(s) | 208 |
| BccI | CCATC | 1 cut(s) | 350 |
| BciT130I | CCWGG | 1 cut(s) | 9 |
| BclI | TGATCA | 1 cut(s) | 508 |
| BfaI | CTAG | 1 cut(s) | 209 |
| BfmI | CTRYAG | 1 cut(s) | 125 |
| BlnI | CCTAGG | 1 cut(s) | 208 |
| Bme1390I | CCNGG | 1 cut(s) | 9 |
| Bme18I | GGWCC | 1 cut(s) | 235 |
| BmgT120I | GGNCC | 1 cut(s) | 235 |
| BmiI | GGNNCC | 1 cut(s) | 237 |
| BmrFI | CCNGG | 1 cut(s) | 9 |
| BmsI | GCATC | 1 cut(s) | 46 |
| BpuEI | CTTGAG | 1 cut(s) | 11 |
| BsaBI | GATNNNNATC | 1 cut(s) | 507 |
| BsaJI | CCNNGG | 3 cut(s) | 64, 208, 441 |
| BsaWI | WCCGGW | 1 cut(s) | 481 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 127, 224 |
| Bse8I | GATNNNNATC | 1 cut(s) | 507 |
| BseAI | TCCGGA | 1 cut(s) | 481 |
| BseBI | CCWGG | 1 cut(s) | 9 |
| BseDI | CCNNGG | 3 cut(s) | 64, 208, 441 |
| BseJI | GATNNNNATC | 1 cut(s) | 507 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 127, 224 |
| BseMII | CTCAG | 1 cut(s) | 240 |
| BseRI | GAGGAG | 2 cut(s) | 95, 464 |
| BsiSI | CCGG | 1 cut(s) | 482 |
| BslFI | GGGAC | 1 cut(s) | 221 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 127, 224 |
| BsmFI | GGGAC | 1 cut(s) | 221 |
| BsmI | GAATGC | 1 cut(s) | 59 |
| Bsp13I | TCCGGA | 1 cut(s) | 481 |
| Bsp143I | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| Bsp19I | CCATGG | 1 cut(s) | 64 |
| BspCNI | CTCAG | 1 cut(s) | 241 |
| BspEI | TCCGGA | 1 cut(s) | 481 |
| BspLI | GGNNCC | 1 cut(s) | 237 |
| BspMAI | CTGCAG | 1 cut(s) | 129 |
| BspPI | GGATC | 4 cut(s) | 188, 199, 429, 466 |
| BssECI | CCNNGG | 3 cut(s) | 64, 208, 441 |
| BssMI | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| BssT1I | CCWWGG | 2 cut(s) | 64, 208 |
| Bst2UI | CCWGG | 1 cut(s) | 9 |
| Bst6I | CTCTTC | 2 cut(s) | 158, 441 |
| BstC8I | GCNNGC | 2 cut(s) | 129, 219 |
| BstDEI | CTNAG | 2 cut(s) | 2, 249 |
| BstDSI | CCRYGG | 1 cut(s) | 64 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 125 |
| BstKTI | GATC | 5 cut(s) | 183, 207, 424, 461, 511 |
| BstMBI | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 476 |
| BstNI | CCWGG | 1 cut(s) | 9 |
| BstSCI | CCNGG | 1 cut(s) | 7 |
| BstSFI | CTRYAG | 1 cut(s) | 125 |
| BstX2I | RGATCY | 2 cut(s) | 180, 204 |
| BstYI | RGATCY | 2 cut(s) | 180, 204 |
| BtgI | CCRYGG | 1 cut(s) | 64 |
| Cac8I | GCNNGC | 2 cut(s) | 129, 219 |
| Cfr13I | GGNCC | 1 cut(s) | 235 |
| CviAII | CATG | 2 cut(s) | 65, 140 |
| CviJI | RGCY | 6 cut(s) | 92, 317, 329, 355, 412, 479 |
| CviKI_1 | RGCY | 6 cut(s) | 92, 317, 329, 355, 412, 479 |
| DdeI | CTNAG | 2 cut(s) | 2, 249 |
| DpnI | GATC | 5 cut(s) | 182, 206, 423, 460, 510 |
| DpnII | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| Eam1104I | CTCTTC | 2 cut(s) | 158, 441 |
| EarI | CTCTTC | 2 cut(s) | 158, 441 |
| Eco130I | CCWWGG | 2 cut(s) | 64, 208 |
| Eco47I | GGWCC | 1 cut(s) | 235 |
| Eco57I | CTGAAG | 1 cut(s) | 434 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 125 |
| EcoRI | GAATTC | 1 cut(s) | 514 |
| EcoRII | CCWGG | 1 cut(s) | 7 |
| EcoT14I | CCWWGG | 2 cut(s) | 64, 208 |
| EcoT22I | ATGCAT | 1 cut(s) | 61 |
| ErhI | CCWWGG | 2 cut(s) | 64, 208 |
| FaeI | CATG | 2 cut(s) | 68, 143 |
| FaiI | YATR | 5 cut(s) | 66, 77, 133, 141, 259 |
| FaqI | GGGAC | 1 cut(s) | 221 |
| FatI | CATG | 2 cut(s) | 64, 139 |
| FbaI | TGATCA | 1 cut(s) | 508 |
| FspBI | CTAG | 1 cut(s) | 209 |
| HapII | CCGG | 1 cut(s) | 482 |
| Hin1II | CATG | 2 cut(s) | 68, 143 |
| HinfI | GANTC | 3 cut(s) | 4, 431, 437 |
| HpaII | CCGG | 1 cut(s) | 482 |
| HphI | GGTGA | 2 cut(s) | 80, 239 |
| Hpy188I | TCNGA | 8 cut(s) | 103, 250, 268, 436, 442, 463, 513, 520 |
| Hpy188III | TCNNGA | 5 cut(s) | 178, 184, 277, 425, 482 |
| HpyAV | CCTTC | 4 cut(s) | 362, 376, 409, 479 |
| HpyCH4V | TGCA | 4 cut(s) | 59, 127, 257, 334 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 476 |
| HpyF3I | CTNAG | 2 cut(s) | 2, 249 |
| Hsp92II | CATG | 2 cut(s) | 68, 143 |
| Kpn2I | TCCGGA | 1 cut(s) | 481 |
| Ksp22I | TGATCA | 1 cut(s) | 508 |
| Kzo9I | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| LmnI | GCTCC | 1 cut(s) | 484 |
| LpnPI | CCDG | 5 cut(s) | 21, 113, 163, 231, 495 |
| LweI | GCATC | 1 cut(s) | 46 |
| MaeI | CTAG | 1 cut(s) | 209 |
| MaeIII | GTNAC | 1 cut(s) | 68 |
| MalI | GATC | 5 cut(s) | 182, 206, 423, 460, 510 |
| MboI | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| MboII | GAAGA | 5 cut(s) | 145, 148, 181, 391, 458 |
| MflI | RGATCY | 2 cut(s) | 180, 204 |
| MluCI | AATT | 4 cut(s) | 173, 269, 303, 514 |
| MlyI | GAGTC | 1 cut(s) | 13 |
| Mph1103I | ATGCAT | 1 cut(s) | 61 |
| MroI | TCCGGA | 1 cut(s) | 481 |
| MroXI | GAANNNNTTC | 1 cut(s) | 173 |
| MseI | TTAA | 1 cut(s) | 306 |
| MslI | CAYNNNNRTG | 1 cut(s) | 144 |
| MspI | CCGG | 1 cut(s) | 482 |
| MspR9I | CCNGG | 1 cut(s) | 9 |
| Mva1269I | GAATGC | 1 cut(s) | 59 |
| MvaI | CCWGG | 1 cut(s) | 9 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 476 |
| NcoI | CCATGG | 1 cut(s) | 64 |
| NdeII | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| NlaIII | CATG | 2 cut(s) | 68, 143 |
| NlaIV | GGNNCC | 1 cut(s) | 237 |
| NmuCI | GTSAC | 1 cut(s) | 68 |
| NsiI | ATGCAT | 1 cut(s) | 61 |
| PctI | GAATGC | 1 cut(s) | 59 |
| PdmI | GAANNNNTTC | 1 cut(s) | 173 |
| PfeI | GAWTC | 2 cut(s) | 431, 437 |
| PfoI | TCCNGGA | 1 cut(s) | 7 |
| PleI | GAGTC | 1 cut(s) | 12 |
| PpsI | GAGTC | 1 cut(s) | 12 |
| Psp6I | CCWGG | 1 cut(s) | 7 |
| PspGI | CCWGG | 1 cut(s) | 7 |
| PspN4I | GGNNCC | 1 cut(s) | 237 |
| PspPI | GGNCC | 1 cut(s) | 235 |
| PstI | CTGCAG | 1 cut(s) | 129 |
| PsuI | RGATCY | 2 cut(s) | 180, 204 |
| RseI | CAYNNNNRTG | 1 cut(s) | 144 |
| SaqAI | TTAA | 1 cut(s) | 306 |
| Sau3AI | GATC | 5 cut(s) | 180, 204, 421, 458, 508 |
| Sau96I | GGNCC | 1 cut(s) | 235 |
| SchI | GAGTC | 1 cut(s) | 13 |
| ScrFI | CCNGG | 1 cut(s) | 9 |
| SetI | ASST | 6 cut(s) | 153, 229, 319, 331, 414, 481 |
| SfaNI | GCATC | 1 cut(s) | 46 |
| SfcI | CTRYAG | 1 cut(s) | 125 |
| SinI | GGWCC | 1 cut(s) | 235 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 144 |
| SmlI | CTYRAG | 1 cut(s) | 26 |
| SmoI | CTYRAG | 1 cut(s) | 26 |
| Sse9I | AATT | 4 cut(s) | 173, 269, 303, 514 |
| SspI | AATATT | 1 cut(s) | 49 |
| SspMI | CTAG | 1 cut(s) | 209 |
| StyD4I | CCNGG | 1 cut(s) | 7 |
| StyI | CCWWGG | 2 cut(s) | 64, 208 |
| TaqI | TCGA | 3 cut(s) | 185, 276, 424 |
| TasI | AATT | 4 cut(s) | 173, 269, 303, 514 |
| TfiI | GAWTC | 2 cut(s) | 431, 437 |
| Tru1I | TTAA | 1 cut(s) | 306 |
| Tru9I | TTAA | 1 cut(s) | 306 |
| TseFI | GTSAC | 1 cut(s) | 68 |
| Tsp45I | GTSAC | 1 cut(s) | 68 |
| TspDTI | ATGAA | 3 cut(s) | 100, 156, 480 |
| VpaK11BI | GGWCC | 1 cut(s) | 235 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 125 |
| XapI | RAATTY | 3 cut(s) | 173, 269, 514 |
| XmaJI | CCTAGG | 1 cut(s) | 208 |
| XmnI | GAANNNNTTC | 1 cut(s) | 173 |
| XspI | CTAG | 1 cut(s) | 209 |
| Zsp2I | ATGCAT | 1 cut(s) | 61 |