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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 421 |
| AciI | CCGC | 1 cut(s) | 302 |
| AcsI | RAATTY | 1 cut(s) | 193 |
| AgsI | TTSAA | 2 cut(s) | 88, 198 |
| AjnI | CCWGG | 1 cut(s) | 265 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 102, 134 |
| AluBI | AGCT | 1 cut(s) | 55 |
| AluI | AGCT | 1 cut(s) | 55 |
| AoxI | GGCC | 1 cut(s) | 330 |
| ApeKI | GCWGC | 1 cut(s) | 452 |
| ApoI | RAATTY | 1 cut(s) | 193 |
| AspLEI | GCGC | 1 cut(s) | 434 |
| AspS9I | GGNCC | 1 cut(s) | 331 |
| AsuC2I | CCSGG | 1 cut(s) | 396 |
| AsuHPI | GGTGA | 2 cut(s) | 281, 291 |
| BbvI | GCAGC | 1 cut(s) | 439 |
| BccI | CCATC | 1 cut(s) | 220 |
| BciT130I | CCWGG | 1 cut(s) | 267 |
| BcnI | CCSGG | 1 cut(s) | 396 |
| BfaI | CTAG | 1 cut(s) | 306 |
| BfuAI | ACCTGC | 1 cut(s) | 421 |
| BisI | GCNGC | 1 cut(s) | 453 |
| BlsI | GCNGC | 1 cut(s) | 454 |
| Bme1390I | CCNGG | 2 cut(s) | 267, 396 |
| BmgT120I | GGNCC | 1 cut(s) | 331 |
| BmiI | GGNNCC | 1 cut(s) | 333 |
| BmrFI | CCNGG | 2 cut(s) | 267, 396 |
| BmsI | GCATC | 1 cut(s) | 443 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 170, 202 |
| BpmI | CTGGAG | 1 cut(s) | 77 |
| BpuEI | CTTGAG | 2 cut(s) | 321, 366 |
| BpuMI | CCSGG | 1 cut(s) | 396 |
| BsaJI | CCNNGG | 1 cut(s) | 395 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 277, 307 |
| BseBI | CCWGG | 1 cut(s) | 267 |
| BseDI | CCNNGG | 1 cut(s) | 395 |
| BseMII | CTCAG | 1 cut(s) | 429 |
| BseRI | GAGGAG | 2 cut(s) | 279, 282 |
| BseXI | GCAGC | 1 cut(s) | 439 |
| BsgI | GTGCAG | 1 cut(s) | 438 |
| BshFI | GGCC | 1 cut(s) | 332 |
| BsiSI | CCGG | 1 cut(s) | 395 |
| BslFI | GGGAC | 1 cut(s) | 412 |
| BsmFI | GGGAC | 1 cut(s) | 412 |
| BsnI | GGCC | 1 cut(s) | 332 |
| BspACI | CCGC | 1 cut(s) | 302 |
| BspANI | GGCC | 1 cut(s) | 332 |
| BspCNI | CTCAG | 1 cut(s) | 430 |
| BspLI | GGNNCC | 1 cut(s) | 333 |
| BspMI | ACCTGC | 1 cut(s) | 421 |
| BssECI | CCNNGG | 1 cut(s) | 395 |
| Bst2UI | CCWGG | 1 cut(s) | 267 |
| Bst6I | CTCTTC | 2 cut(s) | 66, 129 |
| BstC8I | GCNNGC | 1 cut(s) | 430 |
| BstDEI | CTNAG | 2 cut(s) | 237, 438 |
| BstHHI | GCGC | 1 cut(s) | 434 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 250 |
| BstNI | CCWGG | 1 cut(s) | 267 |
| BstSCI | CCNGG | 2 cut(s) | 265, 394 |
| BstV1I | GCAGC | 1 cut(s) | 439 |
| BsuRI | GGCC | 1 cut(s) | 332 |
| BveI | ACCTGC | 1 cut(s) | 421 |
| Cac8I | GCNNGC | 1 cut(s) | 430 |
| CfoI | GCGC | 1 cut(s) | 434 |
| Cfr13I | GGNCC | 1 cut(s) | 331 |
| CviAII | CATG | 1 cut(s) | 245 |
| CviJI | RGCY | 4 cut(s) | 55, 185, 332, 346 |
| CviKI_1 | RGCY | 4 cut(s) | 55, 185, 332, 346 |
| DdeI | CTNAG | 2 cut(s) | 237, 438 |
| Eam1104I | CTCTTC | 2 cut(s) | 66, 129 |
| EarI | CTCTTC | 2 cut(s) | 66, 129 |
| EcoO109I | RGGNCCY | 1 cut(s) | 331 |
| EcoRI | GAATTC | 1 cut(s) | 193 |
| EcoRII | CCWGG | 1 cut(s) | 265 |
| FaeI | CATG | 1 cut(s) | 248 |
| FaiI | YATR | 3 cut(s) | 14, 221, 246 |
| FaqI | GGGAC | 1 cut(s) | 412 |
| FatI | CATG | 1 cut(s) | 244 |
| Fnu4HI | GCNGC | 1 cut(s) | 453 |
| Fsp4HI | GCNGC | 1 cut(s) | 453 |
| FspBI | CTAG | 1 cut(s) | 306 |
| GlaI | GCGC | 1 cut(s) | 433 |
| GluI | GCNGC | 1 cut(s) | 453 |
| GsuI | CTGGAG | 1 cut(s) | 77 |
| HaeIII | GGCC | 1 cut(s) | 332 |
| HapII | CCGG | 1 cut(s) | 395 |
| HhaI | GCGC | 1 cut(s) | 434 |
| Hin1II | CATG | 1 cut(s) | 248 |
| Hin6I | GCGC | 1 cut(s) | 432 |
| HinP1I | GCGC | 1 cut(s) | 432 |
| HincII | GTYRAC | 1 cut(s) | 100 |
| HindII | GTYRAC | 1 cut(s) | 100 |
| HinfI | GANTC | 3 cut(s) | 248, 281, 400 |
| HpaII | CCGG | 1 cut(s) | 395 |
| HphI | GGTGA | 2 cut(s) | 281, 291 |
| Hpy166II | GTNNAC | 1 cut(s) | 100 |
| Hpy188I | TCNGA | 1 cut(s) | 439 |
| Hpy188III | TCNNGA | 2 cut(s) | 198, 338 |
| Hpy8I | GTNNAC | 1 cut(s) | 100 |
| HpyAV | CCTTC | 1 cut(s) | 133 |
| HpyCH4V | TGCA | 1 cut(s) | 455 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 250 |
| HpyF3I | CTNAG | 2 cut(s) | 237, 438 |
| Hsp92II | CATG | 1 cut(s) | 248 |
| HspAI | GCGC | 1 cut(s) | 432 |
| LpnPI | CCDG | 6 cut(s) | 41, 203, 252, 279, 408, 426 |
| Lsp1109I | GCAGC | 1 cut(s) | 439 |
| LweI | GCATC | 1 cut(s) | 443 |
| MaeI | CTAG | 1 cut(s) | 306 |
| MboII | GAAGA | 7 cut(s) | 31, 46, 80, 83, 146, 155, 217 |
| MluCI | AATT | 5 cut(s) | 41, 117, 174, 193, 209 |
| MlyI | GAGTC | 3 cut(s) | 257, 275, 394 |
| MnlI | CCTC | 8 cut(s) | 157, 196, 208, 300, 303, 307, 324, 345 |
| MseI | TTAA | 1 cut(s) | 77 |
| MslI | CAYNNNNRTG | 1 cut(s) | 224 |
| MspI | CCGG | 1 cut(s) | 395 |
| MspR9I | CCNGG | 2 cut(s) | 267, 396 |
| MvaI | CCWGG | 1 cut(s) | 267 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 250 |
| NciI | CCSGG | 1 cut(s) | 396 |
| NlaIII | CATG | 1 cut(s) | 248 |
| NlaIV | GGNNCC | 1 cut(s) | 333 |
| PkrI | GCNGC | 1 cut(s) | 454 |
| PleI | GAGTC | 3 cut(s) | 256, 275, 394 |
| PpsI | GAGTC | 3 cut(s) | 256, 275, 394 |
| Psp6I | CCWGG | 1 cut(s) | 265 |
| PspGI | CCWGG | 1 cut(s) | 265 |
| PspN4I | GGNNCC | 1 cut(s) | 333 |
| PspPI | GGNCC | 1 cut(s) | 331 |
| RseI | CAYNNNNRTG | 1 cut(s) | 224 |
| SaqAI | TTAA | 1 cut(s) | 77 |
| SatI | GCNGC | 1 cut(s) | 453 |
| Sau96I | GGNCC | 1 cut(s) | 331 |
| SchI | GAGTC | 3 cut(s) | 257, 275, 394 |
| ScrFI | CCNGG | 2 cut(s) | 267, 396 |
| SetI | ASST | 5 cut(s) | 57, 168, 271, 388, 415 |
| SfaNI | GCATC | 1 cut(s) | 443 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 224 |
| SmlI | CTYRAG | 2 cut(s) | 336, 381 |
| SmoI | CTYRAG | 2 cut(s) | 336, 381 |
| Sse9I | AATT | 5 cut(s) | 41, 117, 174, 193, 209 |
| SsiI | CCGC | 1 cut(s) | 302 |
| SspMI | CTAG | 1 cut(s) | 306 |
| StyD4I | CCNGG | 2 cut(s) | 265, 394 |
| TasI | AATT | 5 cut(s) | 41, 117, 174, 193, 209 |
| Tru1I | TTAA | 1 cut(s) | 77 |
| Tru9I | TTAA | 1 cut(s) | 77 |
| TseI | GCWGC | 1 cut(s) | 452 |
| XapI | RAATTY | 1 cut(s) | 193 |
| XspI | CTAG | 1 cut(s) | 306 |