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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 283 |
| AcsI | RAATTY | 1 cut(s) | 252 |
| AfaI | GTAC | 1 cut(s) | 31 |
| AgsI | TTSAA | 1 cut(s) | 257 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 142 |
| AjiI | CACGTC | 1 cut(s) | 125 |
| AjnI | CCWGG | 1 cut(s) | 127 |
| AoxI | GGCC | 1 cut(s) | 192 |
| ApeKI | GCWGC | 1 cut(s) | 290 |
| ApoI | RAATTY | 1 cut(s) | 252 |
| AspLEI | GCGC | 1 cut(s) | 295 |
| AspS9I | GGNCC | 1 cut(s) | 193 |
| AsuHPI | GGTGA | 3 cut(s) | 35, 42, 143 |
| BbvI | GCAGC | 1 cut(s) | 302 |
| BccI | CCATC | 2 cut(s) | 20, 82 |
| BceAI | ACGGC | 1 cut(s) | 303 |
| BciT130I | CCWGG | 1 cut(s) | 129 |
| BfaI | CTAG | 1 cut(s) | 200 |
| BfuAI | ACCTGC | 1 cut(s) | 283 |
| BisI | GCNGC | 1 cut(s) | 291 |
| BlsI | GCNGC | 1 cut(s) | 292 |
| Bme1390I | CCNGG | 1 cut(s) | 129 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 142 |
| BmgBI | CACGTC | 1 cut(s) | 125 |
| BmgT120I | GGNCC | 1 cut(s) | 193 |
| BmrFI | CCNGG | 1 cut(s) | 129 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 32, 64 |
| BpuEI | CTTGAG | 1 cut(s) | 228 |
| BsaJI | CCNNGG | 1 cut(s) | 285 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 139, 169 |
| BseBI | CCWGG | 1 cut(s) | 129 |
| BseDI | CCNNGG | 1 cut(s) | 285 |
| BseGI | GGATG | 1 cut(s) | 295 |
| BseRI | GAGGAG | 2 cut(s) | 141, 144 |
| BseXI | GCAGC | 1 cut(s) | 302 |
| BshFI | GGCC | 1 cut(s) | 194 |
| BsnI | GGCC | 1 cut(s) | 194 |
| BspANI | GGCC | 1 cut(s) | 194 |
| BspMI | ACCTGC | 1 cut(s) | 283 |
| BssECI | CCNNGG | 1 cut(s) | 285 |
| Bst2UI | CCWGG | 1 cut(s) | 129 |
| BstC8I | GCNNGC | 1 cut(s) | 221 |
| BstDEI | CTNAG | 2 cut(s) | 99, 167 |
| BstDSI | CCRYGG | 1 cut(s) | 285 |
| BstF5I | GGATG | 1 cut(s) | 295 |
| BstHHI | GCGC | 1 cut(s) | 295 |
| BstNI | CCWGG | 1 cut(s) | 129 |
| BstSCI | CCNGG | 1 cut(s) | 127 |
| BstV1I | GCAGC | 1 cut(s) | 302 |
| BsuRI | GGCC | 1 cut(s) | 194 |
| BtgI | CCRYGG | 1 cut(s) | 285 |
| BtrI | CACGTC | 1 cut(s) | 125 |
| BtsCI | GGATG | 1 cut(s) | 295 |
| BveI | ACCTGC | 1 cut(s) | 283 |
| Cac8I | GCNNGC | 1 cut(s) | 221 |
| CfoI | GCGC | 1 cut(s) | 295 |
| Cfr13I | GGNCC | 1 cut(s) | 193 |
| Csp6I | GTAC | 1 cut(s) | 30 |
| CviJI | RGCY | 3 cut(s) | 47, 194, 208 |
| CviKI_1 | RGCY | 3 cut(s) | 47, 194, 208 |
| CviQI | GTAC | 1 cut(s) | 30 |
| DdeI | CTNAG | 2 cut(s) | 99, 167 |
| DriI | GACNNNNNGTC | 1 cut(s) | 142 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 142 |
| EcoRI | GAATTC | 1 cut(s) | 252 |
| EcoRII | CCWGG | 1 cut(s) | 127 |
| FaiI | YATR | 2 cut(s) | 83, 108 |
| Fnu4HI | GCNGC | 1 cut(s) | 291 |
| FokI | GGATG | 1 cut(s) | 282 |
| Fsp4HI | GCNGC | 1 cut(s) | 291 |
| FspBI | CTAG | 1 cut(s) | 200 |
| GlaI | GCGC | 1 cut(s) | 294 |
| GluI | GCNGC | 1 cut(s) | 291 |
| HaeIII | GGCC | 1 cut(s) | 194 |
| HhaI | GCGC | 1 cut(s) | 295 |
| Hin6I | GCGC | 1 cut(s) | 293 |
| HinP1I | GCGC | 1 cut(s) | 293 |
| HincII | GTYRAC | 1 cut(s) | 147 |
| HindII | GTYRAC | 1 cut(s) | 147 |
| HinfI | GANTC | 2 cut(s) | 110, 143 |
| HphI | GGTGA | 3 cut(s) | 35, 42, 143 |
| Hpy166II | GTNNAC | 1 cut(s) | 147 |
| Hpy188I | TCNGA | 1 cut(s) | 300 |
| Hpy188III | TCNNGA | 1 cut(s) | 200 |
| Hpy8I | GTNNAC | 1 cut(s) | 147 |
| HpyCH4IV | ACGT | 1 cut(s) | 124 |
| HpyF3I | CTNAG | 2 cut(s) | 99, 167 |
| HpySE526I | ACGT | 1 cut(s) | 124 |
| HspAI | GCGC | 1 cut(s) | 293 |
| LpnPI | CCDG | 3 cut(s) | 114, 141, 288 |
| Lsp1109I | GCAGC | 1 cut(s) | 302 |
| MaeI | CTAG | 1 cut(s) | 200 |
| MaeII | ACGT | 1 cut(s) | 124 |
| MfeI | CAATTG | 1 cut(s) | 71 |
| MluCI | AATT | 3 cut(s) | 36, 71, 252 |
| MlyI | GAGTC | 2 cut(s) | 119, 152 |
| MnlI | CCTC | 5 cut(s) | 58, 70, 162, 165, 186 |
| MseI | TTAA | 1 cut(s) | 57 |
| MslI | CAYNNNNRTG | 1 cut(s) | 86 |
| MspR9I | CCNGG | 1 cut(s) | 129 |
| MunI | CAATTG | 1 cut(s) | 71 |
| MvaI | CCWGG | 1 cut(s) | 129 |
| PkrI | GCNGC | 1 cut(s) | 292 |
| PleI | GAGTC | 2 cut(s) | 118, 151 |
| PpsI | GAGTC | 2 cut(s) | 118, 151 |
| Psp6I | CCWGG | 1 cut(s) | 127 |
| PspGI | CCWGG | 1 cut(s) | 127 |
| PspPI | GGNCC | 1 cut(s) | 193 |
| RsaI | GTAC | 1 cut(s) | 31 |
| RsaNI | GTAC | 1 cut(s) | 30 |
| RseI | CAYNNNNRTG | 1 cut(s) | 86 |
| SaqAI | TTAA | 1 cut(s) | 57 |
| SatI | GCNGC | 1 cut(s) | 291 |
| Sau96I | GGNCC | 1 cut(s) | 193 |
| SchI | GAGTC | 2 cut(s) | 119, 152 |
| ScrFI | CCNGG | 1 cut(s) | 129 |
| SetI | ASST | 5 cut(s) | 11, 127, 133, 250, 277 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 86 |
| SmlI | CTYRAG | 1 cut(s) | 243 |
| SmoI | CTYRAG | 1 cut(s) | 243 |
| Sse9I | AATT | 3 cut(s) | 36, 71, 252 |
| SspMI | CTAG | 1 cut(s) | 200 |
| StyD4I | CCNGG | 1 cut(s) | 127 |
| TaiI | ACGT | 1 cut(s) | 127 |
| TasI | AATT | 3 cut(s) | 36, 71, 252 |
| Tru1I | TTAA | 1 cut(s) | 57 |
| Tru9I | TTAA | 1 cut(s) | 57 |
| TseI | GCWGC | 1 cut(s) | 290 |
| XapI | RAATTY | 1 cut(s) | 252 |
| XbaI | TCTAGA | 1 cut(s) | 199 |
| XspI | CTAG | 1 cut(s) | 200 |