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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 404 |
| AccIII | TCCGGA | 1 cut(s) | 10 |
| AciI | CCGC | 1 cut(s) | 319 |
| AclWI | GGATC | 1 cut(s) | 275 |
| AcsI | RAATTY | 3 cut(s) | 43, 200, 460 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 325, 480 |
| AgsI | TTSAA | 2 cut(s) | 88, 488 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 410, 442 |
| AluBI | AGCT | 5 cut(s) | 137, 197, 233, 302, 499 |
| AluI | AGCT | 5 cut(s) | 137, 197, 233, 302, 499 |
| AlwI | GGATC | 1 cut(s) | 275 |
| Aor13HI | TCCGGA | 1 cut(s) | 10 |
| ApeKI | GCWGC | 3 cut(s) | 134, 296, 299 |
| ApoI | RAATTY | 3 cut(s) | 43, 200, 460 |
| AsuHPI | GGTGA | 3 cut(s) | 16, 76, 308 |
| BanII | GRGCYC | 2 cut(s) | 113, 381 |
| BauI | CACGAG | 1 cut(s) | 398 |
| BbvI | GCAGC | 3 cut(s) | 146, 308, 311 |
| BccI | CCATC | 2 cut(s) | 360, 408 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 269, 303 |
| BfaI | CTAG | 1 cut(s) | 539 |
| BfmI | CTRYAG | 1 cut(s) | 288 |
| BisI | GCNGC | 3 cut(s) | 135, 297, 300 |
| BlpI | GCTNAGC | 1 cut(s) | 138 |
| BlsI | GCNGC | 3 cut(s) | 136, 298, 301 |
| BmsI | GCATC | 2 cut(s) | 65, 411 |
| BoxI | GACNNNNGTC | 1 cut(s) | 512 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 138 |
| BsaBI | GATNNNNATC | 1 cut(s) | 122 |
| BsaJI | CCNNGG | 1 cut(s) | 262 |
| BsaWI | WCCGGW | 1 cut(s) | 10 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 89, 119 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 325, 480 |
| Bse8I | GATNNNNATC | 1 cut(s) | 122 |
| BseAI | TCCGGA | 1 cut(s) | 10 |
| BseDI | CCNNGG | 1 cut(s) | 262 |
| BseGI | GGATG | 3 cut(s) | 419, 457, 510 |
| BseJI | GATNNNNATC | 1 cut(s) | 122 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 325, 480 |
| BseMII | CTCAG | 1 cut(s) | 152 |
| BseXI | GCAGC | 3 cut(s) | 146, 308, 311 |
| BseYI | CCCAGC | 1 cut(s) | 480 |
| BsgI | GTGCAG | 1 cut(s) | 256 |
| Bsh1285I | CGRYCG | 1 cut(s) | 243 |
| BsiEI | CGRYCG | 1 cut(s) | 243 |
| BsiSI | CCGG | 1 cut(s) | 11 |
| BslFI | GGGAC | 2 cut(s) | 244, 440 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 325, 480 |
| BsmFI | GGGAC | 2 cut(s) | 244, 440 |
| Bsp1286I | GDGCHC | 2 cut(s) | 113, 381 |
| Bsp13I | TCCGGA | 1 cut(s) | 10 |
| Bsp143I | GATC | 1 cut(s) | 267 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 138 |
| Bsp19I | CCATGG | 1 cut(s) | 262 |
| BspACI | CCGC | 1 cut(s) | 319 |
| BspCNI | CTCAG | 1 cut(s) | 151 |
| BspEI | TCCGGA | 1 cut(s) | 10 |
| BspMAI | CTGCAG | 1 cut(s) | 292 |
| BspPI | GGATC | 1 cut(s) | 275 |
| BssECI | CCNNGG | 1 cut(s) | 262 |
| BssMI | GATC | 1 cut(s) | 267 |
| BssNAI | GTATAC | 1 cut(s) | 405 |
| BssSI | CACGAG | 1 cut(s) | 398 |
| BssT1I | CCWWGG | 1 cut(s) | 262 |
| Bst1107I | GTATAC | 1 cut(s) | 405 |
| Bst2BI | CACGAG | 1 cut(s) | 398 |
| Bst4CI | ACNGT | 3 cut(s) | 244, 277, 386 |
| BstDEI | CTNAG | 1 cut(s) | 138 |
| BstDSI | CCRYGG | 1 cut(s) | 262 |
| BstF5I | GGATG | 3 cut(s) | 419, 457, 510 |
| BstKTI | GATC | 1 cut(s) | 270 |
| BstMBI | GATC | 1 cut(s) | 267 |
| BstMCI | CGRYCG | 1 cut(s) | 243 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 296 |
| BstNSI | RCATGY | 2 cut(s) | 395, 538 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 512 |
| BstSFI | CTRYAG | 1 cut(s) | 288 |
| BstV1I | GCAGC | 3 cut(s) | 146, 308, 311 |
| BstX2I | RGATCY | 1 cut(s) | 267 |
| BstYI | RGATCY | 1 cut(s) | 267 |
| BstZ17I | GTATAC | 1 cut(s) | 405 |
| BtgI | CCRYGG | 1 cut(s) | 262 |
| BtsCI | GGATG | 3 cut(s) | 419, 457, 510 |
| CviAII | CATG | 3 cut(s) | 263, 392, 535 |
| CviJI | RGCY | 9 cut(s) | 111, 137, 197, 209, 233, 302, 379, 447, 499 |
| CviKI_1 | RGCY | 9 cut(s) | 111, 137, 197, 209, 233, 302, 379, 447, 499 |
| DdeI | CTNAG | 1 cut(s) | 138 |
| DpnI | GATC | 1 cut(s) | 269 |
| DpnII | GATC | 1 cut(s) | 267 |
| Eco130I | CCWWGG | 1 cut(s) | 262 |
| Eco24I | GRGCYC | 2 cut(s) | 113, 381 |
| EcoT14I | CCWWGG | 1 cut(s) | 262 |
| EcoT38I | GRGCYC | 2 cut(s) | 113, 381 |
| ErhI | CCWWGG | 1 cut(s) | 262 |
| FaeI | CATG | 3 cut(s) | 266, 395, 538 |
| FaiI | YATR | 6 cut(s) | 264, 393, 405, 467, 478, 536 |
| FaqI | GGGAC | 2 cut(s) | 244, 440 |
| FatI | CATG | 3 cut(s) | 262, 391, 534 |
| FblI | GTMKAC | 1 cut(s) | 404 |
| Fnu4HI | GCNGC | 3 cut(s) | 135, 297, 300 |
| FokI | GGATG | 3 cut(s) | 426, 464, 517 |
| FriOI | GRGCYC | 2 cut(s) | 113, 381 |
| Fsp4HI | GCNGC | 3 cut(s) | 135, 297, 300 |
| FspBI | CTAG | 1 cut(s) | 539 |
| GluI | GCNGC | 3 cut(s) | 135, 297, 300 |
| GsaI | CCCAGC | 1 cut(s) | 484 |
| HapII | CCGG | 1 cut(s) | 11 |
| Hin1II | CATG | 3 cut(s) | 266, 395, 538 |
| HinfI | GANTC | 7 cut(s) | 7, 118, 149, 284, 364, 437, 469 |
| HpaII | CCGG | 1 cut(s) | 11 |
| HphI | GGTGA | 3 cut(s) | 16, 76, 308 |
| Hpy166II | GTNNAC | 2 cut(s) | 389, 405 |
| Hpy188I | TCNGA | 1 cut(s) | 376 |
| Hpy188III | TCNNGA | 3 cut(s) | 11, 398, 411 |
| Hpy8I | GTNNAC | 2 cut(s) | 389, 405 |
| Hpy99I | CGWCG | 1 cut(s) | 248 |
| HpyAV | CCTTC | 3 cut(s) | 61, 82, 245 |
| HpyCH4III | ACNGT | 3 cut(s) | 244, 277, 386 |
| HpyCH4V | TGCA | 3 cut(s) | 56, 273, 290 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 296 |
| HpyF3I | CTNAG | 1 cut(s) | 138 |
| Hsp92II | CATG | 3 cut(s) | 266, 395, 538 |
| Kpn2I | TCCGGA | 1 cut(s) | 10 |
| Kzo9I | GATC | 1 cut(s) | 267 |
| LmnI | GCTCC | 1 cut(s) | 97 |
| LpnPI | CCDG | 2 cut(s) | 24, 466 |
| Lsp1109I | GCAGC | 3 cut(s) | 146, 308, 311 |
| LweI | GCATC | 2 cut(s) | 65, 411 |
| MaeI | CTAG | 1 cut(s) | 539 |
| MaeIII | GTNAC | 1 cut(s) | 34 |
| MalI | GATC | 1 cut(s) | 269 |
| MboI | GATC | 1 cut(s) | 267 |
| MboII | GAAGA | 4 cut(s) | 185, 293, 343, 430 |
| MflI | RGATCY | 1 cut(s) | 267 |
| MhlI | GDGCHC | 2 cut(s) | 113, 381 |
| MluCI | AATT | 5 cut(s) | 43, 83, 92, 200, 460 |
| MlyI | GAGTC | 2 cut(s) | 143, 358 |
| MmeI | TCCRAC | 1 cut(s) | 408 |
| MnlI | CCTC | 4 cut(s) | 7, 241, 324, 359 |
| MroI | TCCGGA | 1 cut(s) | 10 |
| MseI | TTAA | 1 cut(s) | 204 |
| MspI | CCGG | 1 cut(s) | 11 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 296 |
| NcoI | CCATGG | 1 cut(s) | 262 |
| NdeII | GATC | 1 cut(s) | 267 |
| NlaIII | CATG | 3 cut(s) | 266, 395, 538 |
| NmuCI | GTSAC | 1 cut(s) | 34 |
| NspI | RCATGY | 2 cut(s) | 395, 538 |
| PfeI | GAWTC | 5 cut(s) | 7, 118, 284, 437, 469 |
| PkrI | GCNGC | 3 cut(s) | 136, 298, 301 |
| PleI | GAGTC | 2 cut(s) | 143, 358 |
| PpsI | GAGTC | 2 cut(s) | 143, 358 |
| PshAI | GACNNNNGTC | 1 cut(s) | 512 |
| PspFI | CCCAGC | 1 cut(s) | 480 |
| PstI | CTGCAG | 1 cut(s) | 292 |
| PsuI | RGATCY | 1 cut(s) | 267 |
| SaqAI | TTAA | 1 cut(s) | 204 |
| SatI | GCNGC | 3 cut(s) | 135, 297, 300 |
| Sau3AI | GATC | 1 cut(s) | 267 |
| SchI | GAGTC | 2 cut(s) | 143, 358 |
| SduI | GDGCHC | 2 cut(s) | 113, 381 |
| SetI | ASST | 9 cut(s) | 139, 163, 199, 235, 256, 304, 316, 351, 501 |
| SfaNI | GCATC | 2 cut(s) | 65, 411 |
| SfcI | CTRYAG | 1 cut(s) | 288 |
| Sse9I | AATT | 5 cut(s) | 43, 83, 92, 200, 460 |
| SsiI | CCGC | 1 cut(s) | 319 |
| SspMI | CTAG | 1 cut(s) | 539 |
| StyI | CCWWGG | 1 cut(s) | 262 |
| TaaI | ACNGT | 3 cut(s) | 244, 277, 386 |
| TaqI | TCGA | 3 cut(s) | 239, 246, 279 |
| TaqII | GACCGA | 1 cut(s) | 53 |
| TasI | AATT | 5 cut(s) | 43, 83, 92, 200, 460 |
| TfiI | GAWTC | 5 cut(s) | 7, 118, 284, 437, 469 |
| Tru1I | TTAA | 1 cut(s) | 204 |
| Tru9I | TTAA | 1 cut(s) | 204 |
| TseFI | GTSAC | 1 cut(s) | 34 |
| TseI | GCWGC | 3 cut(s) | 134, 296, 299 |
| Tsp45I | GTSAC | 1 cut(s) | 34 |
| TspDTI | ATGAA | 1 cut(s) | 482 |
| XapI | RAATTY | 3 cut(s) | 43, 200, 460 |
| XceI | RCATGY | 2 cut(s) | 395, 538 |
| XmiI | GTMKAC | 1 cut(s) | 404 |
| XspI | CTAG | 1 cut(s) | 539 |