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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 578 |
| AccI | GTMKAC | 1 cut(s) | 431 |
| AciI | CCGC | 3 cut(s) | 135, 156, 167 |
| AcsI | RAATTY | 3 cut(s) | 43, 531, 684 |
| AfaI | GTAC | 1 cut(s) | 325 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 173, 606, 670 |
| AflIII | ACRYGT | 1 cut(s) | 274 |
| AgsI | TTSAA | 3 cut(s) | 89, 305, 466 |
| AjiI | CACGTC | 1 cut(s) | 233 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 203, 235 |
| ApoI | RAATTY | 3 cut(s) | 43, 531, 684 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 312 |
| AsuHPI | GGTGA | 2 cut(s) | 156, 421 |
| BaeGI | GKGCMC | 1 cut(s) | 458 |
| BanII | GRGCYC | 2 cut(s) | 114, 229 |
| BbsI | GAAGAC | 3 cut(s) | 150, 356, 450 |
| BccI | CCATC | 2 cut(s) | 208, 433 |
| BceAI | ACGGC | 1 cut(s) | 148 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 499, 533 |
| BclI | TGATCA | 1 cut(s) | 486 |
| BfmI | CTRYAG | 1 cut(s) | 297 |
| BisI | GCNGC | 1 cut(s) | 136 |
| BlsI | GCNGC | 1 cut(s) | 137 |
| BmgBI | CACGTC | 1 cut(s) | 233 |
| BmiI | GGNNCC | 1 cut(s) | 221 |
| BmrI | ACTGGG | 1 cut(s) | 444 |
| BmsI | GCATC | 3 cut(s) | 66, 525, 664 |
| BmuI | ACTGGG | 1 cut(s) | 444 |
| BoxI | GACNNNNGTC | 1 cut(s) | 638 |
| BpiI | GAAGAC | 3 cut(s) | 150, 356, 450 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 32, 64 |
| BpuEI | CTTGAG | 1 cut(s) | 552 |
| Bsa29I | ATCGAT | 1 cut(s) | 543 |
| BsaJI | CCNNGG | 1 cut(s) | 244 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 90, 120 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 173, 606, 670 |
| Bse1I | ACTGG | 1 cut(s) | 439 |
| BseCI | ATCGAT | 1 cut(s) | 543 |
| BseDI | CCNNGG | 1 cut(s) | 244 |
| BseGI | GGATG | 5 cut(s) | 444, 499, 583, 636, 679 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 173, 606, 670 |
| BseMII | CTCAG | 1 cut(s) | 153 |
| BseNI | ACTGG | 1 cut(s) | 439 |
| BseSI | GKGCMC | 1 cut(s) | 458 |
| BseYI | CCCAGC | 1 cut(s) | 606 |
| Bsh1285I | CGRYCG | 2 cut(s) | 547, 551 |
| BshVI | ATCGAT | 1 cut(s) | 543 |
| BsiEI | CGRYCG | 2 cut(s) | 547, 551 |
| BsiSI | CCGG | 1 cut(s) | 664 |
| BslFI | GGGAC | 1 cut(s) | 566 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 173, 606, 670 |
| BsmFI | GGGAC | 1 cut(s) | 566 |
| Bsp1286I | GDGCHC | 3 cut(s) | 114, 229, 458 |
| Bsp143I | GATC | 3 cut(s) | 486, 540, 544 |
| BspACI | CCGC | 3 cut(s) | 135, 156, 167 |
| BspCNI | CTCAG | 1 cut(s) | 152 |
| BspDI | ATCGAT | 1 cut(s) | 543 |
| BspLI | GGNNCC | 1 cut(s) | 221 |
| BsrI | ACTGG | 1 cut(s) | 439 |
| BssECI | CCNNGG | 1 cut(s) | 244 |
| BssMI | GATC | 3 cut(s) | 486, 540, 544 |
| BssNAI | GTATAC | 1 cut(s) | 432 |
| Bst1107I | GTATAC | 1 cut(s) | 432 |
| Bst4CI | ACNGT | 5 cut(s) | 173, 219, 248, 417, 449 |
| BstC8I | GCNNGC | 2 cut(s) | 363, 508 |
| BstDEI | CTNAG | 1 cut(s) | 139 |
| BstDSI | CCRYGG | 1 cut(s) | 244 |
| BstF5I | GGATG | 5 cut(s) | 444, 499, 583, 636, 679 |
| BstKTI | GATC | 3 cut(s) | 489, 543, 547 |
| BstMBI | GATC | 3 cut(s) | 486, 540, 544 |
| BstMCI | CGRYCG | 2 cut(s) | 547, 551 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 371 |
| BstNSI | RCATGY | 3 cut(s) | 242, 278, 663 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 638 |
| BstSFI | CTRYAG | 1 cut(s) | 297 |
| BstSLI | GKGCMC | 1 cut(s) | 458 |
| BstV2I | GAAGAC | 3 cut(s) | 150, 356, 450 |
| BstZ17I | GTATAC | 1 cut(s) | 432 |
| Bsu15I | ATCGAT | 1 cut(s) | 543 |
| BsuTUI | ATCGAT | 1 cut(s) | 543 |
| BtgI | CCRYGG | 1 cut(s) | 244 |
| BtrI | CACGTC | 1 cut(s) | 233 |
| BtsCI | GGATG | 5 cut(s) | 444, 499, 583, 636, 679 |
| Cac8I | GCNNGC | 2 cut(s) | 363, 508 |
| ClaI | ATCGAT | 1 cut(s) | 543 |
| Csp6I | GTAC | 1 cut(s) | 324 |
| CviAII | CATG | 6 cut(s) | 239, 275, 484, 490, 616, 660 |
| CviJI | RGCY | 5 cut(s) | 112, 138, 150, 227, 270 |
| CviKI_1 | RGCY | 5 cut(s) | 112, 138, 150, 227, 270 |
| CviQI | GTAC | 1 cut(s) | 324 |
| DdeI | CTNAG | 1 cut(s) | 139 |
| DpnI | GATC | 3 cut(s) | 488, 542, 546 |
| DpnII | GATC | 3 cut(s) | 486, 540, 544 |
| DraI | TTTAAA | 1 cut(s) | 477 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 578 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 578 |
| Eco24I | GRGCYC | 2 cut(s) | 114, 229 |
| EcoRI | GAATTC | 1 cut(s) | 531 |
| EcoT38I | GRGCYC | 2 cut(s) | 114, 229 |
| FaeI | CATG | 6 cut(s) | 242, 278, 487, 493, 619, 663 |
| FaqI | GGGAC | 1 cut(s) | 566 |
| FatI | CATG | 6 cut(s) | 238, 274, 483, 489, 615, 659 |
| FauI | CCCGC | 1 cut(s) | 163 |
| FbaI | TGATCA | 1 cut(s) | 486 |
| FblI | GTMKAC | 1 cut(s) | 431 |
| Fnu4HI | GCNGC | 1 cut(s) | 136 |
| FokI | GGATG | 5 cut(s) | 451, 506, 590, 643, 686 |
| FriOI | GRGCYC | 2 cut(s) | 114, 229 |
| Fsp4HI | GCNGC | 1 cut(s) | 136 |
| GluI | GCNGC | 1 cut(s) | 136 |
| GsaI | CCCAGC | 1 cut(s) | 610 |
| HapII | CCGG | 1 cut(s) | 664 |
| Hin1II | CATG | 6 cut(s) | 242, 278, 487, 493, 619, 663 |
| HincII | GTYRAC | 1 cut(s) | 613 |
| HindII | GTYRAC | 1 cut(s) | 613 |
| HinfI | GANTC | 2 cut(s) | 212, 595 |
| HpaI | GTTAAC | 1 cut(s) | 613 |
| HpaII | CCGG | 1 cut(s) | 664 |
| HphI | GGTGA | 2 cut(s) | 156, 421 |
| Hpy166II | GTNNAC | 4 cut(s) | 236, 380, 432, 613 |
| Hpy188I | TCNGA | 2 cut(s) | 142, 224 |
| Hpy188III | TCNNGA | 3 cut(s) | 466, 538, 569 |
| Hpy8I | GTNNAC | 4 cut(s) | 236, 380, 432, 613 |
| HpyAV | CCTTC | 1 cut(s) | 62 |
| HpyCH4III | ACNGT | 5 cut(s) | 173, 219, 248, 417, 449 |
| HpyCH4IV | ACGT | 1 cut(s) | 232 |
| HpyCH4V | TGCA | 3 cut(s) | 57, 387, 516 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 371 |
| HpyF3I | CTNAG | 1 cut(s) | 139 |
| HpySE526I | ACGT | 1 cut(s) | 232 |
| Hsp92II | CATG | 6 cut(s) | 242, 278, 487, 493, 619, 663 |
| Ksp22I | TGATCA | 1 cut(s) | 486 |
| KspAI | GTTAAC | 1 cut(s) | 613 |
| Kzo9I | GATC | 3 cut(s) | 486, 540, 544 |
| LmnI | GCTCC | 1 cut(s) | 98 |
| LpnPI | CCDG | 5 cut(s) | 420, 444, 592, 655, 677 |
| LweI | GCATC | 3 cut(s) | 66, 525, 664 |
| MaeII | ACGT | 1 cut(s) | 232 |
| MaeIII | GTNAC | 1 cut(s) | 34 |
| MalI | GATC | 3 cut(s) | 488, 542, 546 |
| MboI | GATC | 3 cut(s) | 486, 540, 544 |
| MboII | GAAGA | 6 cut(s) | 155, 191, 317, 358, 361, 455 |
| MfeI | CAATTG | 1 cut(s) | 511 |
| MhlI | GDGCHC | 3 cut(s) | 114, 229, 458 |
| MluCI | AATT | 6 cut(s) | 43, 84, 93, 511, 531, 684 |
| MmeI | TCCRAC | 1 cut(s) | 534 |
| MnlI | CCTC | 5 cut(s) | 7, 137, 161, 172, 245 |
| MroXI | GAANNNNTTC | 1 cut(s) | 312 |
| MseI | TTAA | 2 cut(s) | 476, 612 |
| MslI | CAYNNNNRTG | 1 cut(s) | 488 |
| MspI | CCGG | 1 cut(s) | 664 |
| MunI | CAATTG | 1 cut(s) | 511 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 371 |
| NdeII | GATC | 3 cut(s) | 486, 540, 544 |
| NlaIII | CATG | 6 cut(s) | 242, 278, 487, 493, 619, 663 |
| NlaIV | GGNNCC | 1 cut(s) | 221 |
| NmuCI | GTSAC | 1 cut(s) | 34 |
| NspI | RCATGY | 3 cut(s) | 242, 278, 663 |
| PciI | ACATGT | 1 cut(s) | 274 |
| PdmI | GAANNNNTTC | 1 cut(s) | 312 |
| PfeI | GAWTC | 2 cut(s) | 212, 595 |
| PkrI | GCNGC | 1 cut(s) | 137 |
| Ple19I | CGATCG | 1 cut(s) | 547 |
| PscI | ACATGT | 1 cut(s) | 274 |
| PshAI | GACNNNNGTC | 1 cut(s) | 638 |
| PspFI | CCCAGC | 1 cut(s) | 606 |
| PspN4I | GGNNCC | 1 cut(s) | 221 |
| PvuI | CGATCG | 1 cut(s) | 547 |
| RsaI | GTAC | 1 cut(s) | 325 |
| RsaNI | GTAC | 1 cut(s) | 324 |
| RseI | CAYNNNNRTG | 1 cut(s) | 488 |
| SaqAI | TTAA | 2 cut(s) | 476, 612 |
| SatI | GCNGC | 1 cut(s) | 136 |
| Sau3AI | GATC | 3 cut(s) | 486, 540, 544 |
| SduI | GDGCHC | 3 cut(s) | 114, 229, 458 |
| SetI | ASST | 5 cut(s) | 129, 164, 235, 392, 463 |
| SfaNI | GCATC | 3 cut(s) | 66, 525, 664 |
| SfcI | CTRYAG | 1 cut(s) | 297 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 488 |
| SmlI | CTYRAG | 1 cut(s) | 567 |
| SmoI | CTYRAG | 1 cut(s) | 567 |
| Sse9I | AATT | 6 cut(s) | 43, 84, 93, 511, 531, 684 |
| SsiI | CCGC | 3 cut(s) | 135, 156, 167 |
| TaaI | ACNGT | 5 cut(s) | 173, 219, 248, 417, 449 |
| TaiI | ACGT | 1 cut(s) | 235 |
| TaqI | TCGA | 4 cut(s) | 191, 281, 519, 543 |
| TaqII | GACCGA | 2 cut(s) | 53, 537 |
| TasI | AATT | 6 cut(s) | 43, 84, 93, 511, 531, 684 |
| TauI | GCSGC | 1 cut(s) | 138 |
| TfiI | GAWTC | 2 cut(s) | 212, 595 |
| Tru1I | TTAA | 2 cut(s) | 476, 612 |
| Tru9I | TTAA | 2 cut(s) | 476, 612 |
| TseFI | GTSAC | 1 cut(s) | 34 |
| Tsp45I | GTSAC | 1 cut(s) | 34 |
| TspDTI | ATGAA | 2 cut(s) | 510, 608 |
| TspGWI | ACGGA | 2 cut(s) | 26, 485 |
| XapI | RAATTY | 3 cut(s) | 43, 531, 684 |
| XceI | RCATGY | 3 cut(s) | 242, 278, 663 |
| XmiI | GTMKAC | 1 cut(s) | 431 |
| XmnI | GAANNNNTTC | 1 cut(s) | 312 |