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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 124 |
| AclWI | GGATC | 1 cut(s) | 620 |
| AcoI | YGGCCR | 1 cut(s) | 14 |
| AcsI | RAATTY | 1 cut(s) | 388 |
| AcuI | CTGAAG | 2 cut(s) | 198, 227 |
| AfaI | GTAC | 1 cut(s) | 619 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 23 |
| AgsI | TTSAA | 3 cut(s) | 80, 295, 433 |
| AluBI | AGCT | 6 cut(s) | 146, 241, 304, 482, 495, 772 |
| AluI | AGCT | 6 cut(s) | 146, 241, 304, 482, 495, 772 |
| AlwI | GGATC | 1 cut(s) | 620 |
| Aor13HI | TCCGGA | 1 cut(s) | 124 |
| AoxI | GGCC | 1 cut(s) | 14 |
| ApeKI | GCWGC | 3 cut(s) | 479, 640, 643 |
| ApoI | RAATTY | 1 cut(s) | 388 |
| AsuHPI | GGTGA | 3 cut(s) | 38, 361, 421 |
| BanII | GRGCYC | 2 cut(s) | 458, 730 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 165, 197 |
| BbvI | GCAGC | 3 cut(s) | 491, 652, 655 |
| BccI | CCATC | 2 cut(s) | 143, 708 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 613, 647 |
| BfaI | CTAG | 1 cut(s) | 356 |
| BfmI | CTRYAG | 2 cut(s) | 276, 632 |
| BisI | GCNGC | 3 cut(s) | 480, 641, 644 |
| BlsI | GCNGC | 3 cut(s) | 481, 642, 645 |
| BmsI | GCATC | 2 cut(s) | 554, 655 |
| BoxI | GACNNNNGTC | 1 cut(s) | 779 |
| BsaJI | CCNNGG | 2 cut(s) | 84, 607 |
| BsaWI | WCCGGW | 1 cut(s) | 124 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 434, 464 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 23 |
| BseAI | TCCGGA | 1 cut(s) | 124 |
| BseDI | CCNNGG | 2 cut(s) | 84, 607 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 23 |
| BseRI | GAGGAG | 4 cut(s) | 20, 107, 269, 272 |
| BseXI | GCAGC | 3 cut(s) | 491, 652, 655 |
| BsgI | GTGCAG | 1 cut(s) | 651 |
| BshFI | GGCC | 1 cut(s) | 16 |
| BsiSI | CCGG | 2 cut(s) | 17, 125 |
| BslFI | GGGAC | 1 cut(s) | 589 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 23 |
| BsmFI | GGGAC | 1 cut(s) | 589 |
| BsnI | GGCC | 1 cut(s) | 16 |
| Bsp1286I | GDGCHC | 2 cut(s) | 458, 730 |
| Bsp13I | TCCGGA | 1 cut(s) | 124 |
| Bsp1407I | TGTACA | 1 cut(s) | 617 |
| Bsp143I | GATC | 4 cut(s) | 101, 286, 577, 612 |
| Bsp19I | CCATGG | 1 cut(s) | 607 |
| BspANI | GGCC | 1 cut(s) | 16 |
| BspEI | TCCGGA | 1 cut(s) | 124 |
| BspMAI | CTGCAG | 2 cut(s) | 280, 636 |
| BspPI | GGATC | 1 cut(s) | 620 |
| BsrGI | TGTACA | 1 cut(s) | 617 |
| BssECI | CCNNGG | 2 cut(s) | 84, 607 |
| BssMI | GATC | 4 cut(s) | 101, 286, 577, 612 |
| BssT1I | CCWWGG | 1 cut(s) | 607 |
| Bst4CI | ACNGT | 3 cut(s) | 121, 672, 735 |
| Bst6I | CTCTTC | 2 cut(s) | 24, 84 |
| BstAUI | TGTACA | 1 cut(s) | 617 |
| BstDEI | CTNAG | 2 cut(s) | 326, 549 |
| BstDSI | CCRYGG | 1 cut(s) | 607 |
| BstKTI | GATC | 4 cut(s) | 104, 289, 580, 615 |
| BstMBI | GATC | 4 cut(s) | 101, 286, 577, 612 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 640 |
| BstNSI | RCATGY | 1 cut(s) | 744 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 779 |
| BstSFI | CTRYAG | 2 cut(s) | 276, 632 |
| BstV1I | GCAGC | 3 cut(s) | 491, 652, 655 |
| BstX2I | RGATCY | 1 cut(s) | 612 |
| BstYI | RGATCY | 1 cut(s) | 612 |
| BsuRI | GGCC | 1 cut(s) | 16 |
| BtgI | CCRYGG | 1 cut(s) | 607 |
| BtsI | GCAGTG | 1 cut(s) | 663 |
| BtsIMutI | CAGTG | 2 cut(s) | 663, 677 |
| Csp6I | GTAC | 1 cut(s) | 618 |
| CviAII | CATG | 3 cut(s) | 346, 608, 741 |
| CviQI | GTAC | 1 cut(s) | 618 |
| DdeI | CTNAG | 2 cut(s) | 326, 549 |
| DpnI | GATC | 4 cut(s) | 103, 288, 579, 614 |
| DpnII | GATC | 4 cut(s) | 101, 286, 577, 612 |
| EaeI | YGGCCR | 1 cut(s) | 14 |
| Eam1104I | CTCTTC | 2 cut(s) | 24, 84 |
| EarI | CTCTTC | 2 cut(s) | 24, 84 |
| Eco130I | CCWWGG | 1 cut(s) | 607 |
| Eco24I | GRGCYC | 2 cut(s) | 458, 730 |
| Eco57I | CTGAAG | 2 cut(s) | 198, 227 |
| EcoT14I | CCWWGG | 1 cut(s) | 607 |
| EcoT38I | GRGCYC | 2 cut(s) | 458, 730 |
| ErhI | CCWWGG | 1 cut(s) | 607 |
| FaeI | CATG | 3 cut(s) | 349, 611, 744 |
| FaiI | YATR | 7 cut(s) | 13, 270, 272, 347, 492, 609, 742 |
| FaqI | GGGAC | 1 cut(s) | 589 |
| FatI | CATG | 3 cut(s) | 345, 607, 740 |
| Fnu4HI | GCNGC | 3 cut(s) | 480, 641, 644 |
| FriOI | GRGCYC | 2 cut(s) | 458, 730 |
| Fsp4HI | GCNGC | 3 cut(s) | 480, 641, 644 |
| FspBI | CTAG | 1 cut(s) | 356 |
| GluI | GCNGC | 3 cut(s) | 480, 641, 644 |
| HaeIII | GGCC | 1 cut(s) | 16 |
| HapII | CCGG | 2 cut(s) | 17, 125 |
| Hin1II | CATG | 3 cut(s) | 349, 611, 744 |
| HinfI | GANTC | 6 cut(s) | 51, 80, 352, 463, 628, 712 |
| HpaII | CCGG | 2 cut(s) | 17, 125 |
| HphI | GGTGA | 3 cut(s) | 38, 361, 421 |
| Hpy166II | GTNNAC | 1 cut(s) | 738 |
| Hpy188I | TCNGA | 7 cut(s) | 38, 85, 106, 156, 217, 246, 785 |
| Hpy188III | TCNNGA | 3 cut(s) | 125, 356, 723 |
| Hpy8I | GTNNAC | 1 cut(s) | 738 |
| HpyAV | CCTTC | 7 cut(s) | 17, 59, 122, 406, 427, 512, 590 |
| HpyCH4III | ACNGT | 3 cut(s) | 121, 672, 735 |
| HpyCH4V | TGCA | 4 cut(s) | 278, 545, 634, 668 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 640 |
| HpyF3I | CTNAG | 2 cut(s) | 326, 549 |
| Hsp92II | CATG | 3 cut(s) | 349, 611, 744 |
| Kpn2I | TCCGGA | 1 cut(s) | 124 |
| Kzo9I | GATC | 4 cut(s) | 101, 286, 577, 612 |
| LmnI | GCTCC | 3 cut(s) | 143, 442, 500 |
| LpnPI | CCDG | 5 cut(s) | 30, 138, 188, 305, 736 |
| Lsp1109I | GCAGC | 3 cut(s) | 491, 652, 655 |
| LweI | GCATC | 2 cut(s) | 554, 655 |
| MaeI | CTAG | 1 cut(s) | 356 |
| MaeIII | GTNAC | 2 cut(s) | 379, 713 |
| MalI | GATC | 4 cut(s) | 103, 288, 579, 614 |
| MboI | GATC | 4 cut(s) | 101, 286, 577, 612 |
| MboII | GAAGA | 8 cut(s) | 32, 41, 101, 182, 185, 307, 637, 691 |
| MflI | RGATCY | 1 cut(s) | 612 |
| MhlI | GDGCHC | 2 cut(s) | 458, 730 |
| MluCI | AATT | 6 cut(s) | 157, 388, 428, 437, 530, 718 |
| MlyI | GAGTC | 2 cut(s) | 457, 721 |
| MmeI | TCCRAC | 1 cut(s) | 16 |
| MroI | TCCGGA | 1 cut(s) | 124 |
| MseI | TTAA | 1 cut(s) | 533 |
| MspI | CCGG | 2 cut(s) | 17, 125 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 640 |
| NcoI | CCATGG | 1 cut(s) | 607 |
| NdeII | GATC | 4 cut(s) | 101, 286, 577, 612 |
| NlaIII | CATG | 3 cut(s) | 349, 611, 744 |
| NmuCI | GTSAC | 2 cut(s) | 379, 713 |
| NspI | RCATGY | 1 cut(s) | 744 |
| PfeI | GAWTC | 4 cut(s) | 51, 80, 352, 628 |
| PkrI | GCNGC | 3 cut(s) | 481, 642, 645 |
| PleI | GAGTC | 2 cut(s) | 457, 720 |
| PpsI | GAGTC | 2 cut(s) | 457, 720 |
| PshAI | GACNNNNGTC | 1 cut(s) | 779 |
| PstI | CTGCAG | 2 cut(s) | 280, 636 |
| PsuI | RGATCY | 1 cut(s) | 612 |
| RsaI | GTAC | 1 cut(s) | 619 |
| RsaNI | GTAC | 1 cut(s) | 618 |
| SaqAI | TTAA | 1 cut(s) | 533 |
| SatI | GCNGC | 3 cut(s) | 480, 641, 644 |
| Sau3AI | GATC | 4 cut(s) | 101, 286, 577, 612 |
| SchI | GAGTC | 2 cut(s) | 457, 721 |
| SduI | GDGCHC | 2 cut(s) | 458, 730 |
| SfaNI | GCATC | 2 cut(s) | 554, 655 |
| SfcI | CTRYAG | 2 cut(s) | 276, 632 |
| Sse9I | AATT | 6 cut(s) | 157, 388, 428, 437, 530, 718 |
| SspMI | CTAG | 1 cut(s) | 356 |
| StyI | CCWWGG | 1 cut(s) | 607 |
| TaaI | ACNGT | 3 cut(s) | 121, 672, 735 |
| TaqI | TCGA | 3 cut(s) | 584, 623, 691 |
| TaqII | GACCGA | 1 cut(s) | 398 |
| TasI | AATT | 6 cut(s) | 157, 388, 428, 437, 530, 718 |
| TatI | WGTACW | 1 cut(s) | 617 |
| TfiI | GAWTC | 4 cut(s) | 51, 80, 352, 628 |
| Tru1I | TTAA | 1 cut(s) | 533 |
| Tru9I | TTAA | 1 cut(s) | 533 |
| TscAI | CASTG | 2 cut(s) | 670, 677 |
| TseFI | GTSAC | 2 cut(s) | 379, 713 |
| TseI | GCWGC | 3 cut(s) | 479, 640, 643 |
| Tsp45I | GTSAC | 2 cut(s) | 379, 713 |
| TspDTI | ATGAA | 3 cut(s) | 123, 390, 543 |
| TspRI | CASTG | 2 cut(s) | 670, 677 |
| XapI | RAATTY | 1 cut(s) | 388 |
| XbaI | TCTAGA | 1 cut(s) | 355 |
| XceI | RCATGY | 1 cut(s) | 744 |
| XspI | CTAG | 1 cut(s) | 356 |