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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 200 |
| AgsI | TTSAA | 2 cut(s) | 140, 430 |
| AjiI | CACGTC | 1 cut(s) | 605 |
| AjnI | CCWGG | 1 cut(s) | 607 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 248, 280, 444, 476 |
| AluBI | AGCT | 3 cut(s) | 235, 298, 397 |
| AluI | AGCT | 3 cut(s) | 235, 298, 397 |
| Alw26I | GTCTC | 3 cut(s) | 164, 200, 341 |
| Ama87I | CYCGRG | 1 cut(s) | 398 |
| AoxI | GGCC | 1 cut(s) | 672 |
| AspLEI | GCGC | 1 cut(s) | 366 |
| AspS9I | GGNCC | 1 cut(s) | 673 |
| AsuHPI | GGTGA | 3 cut(s) | 175, 332, 623 |
| AvaI | CYCGRG | 1 cut(s) | 398 |
| AxyI | CCTNAGG | 1 cut(s) | 678 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 748, 781 |
| BccI | CCATC | 2 cut(s) | 113, 337 |
| BciT130I | CCWGG | 1 cut(s) | 609 |
| BcoDI | GTCTC | 3 cut(s) | 164, 200, 341 |
| BfaI | CTAG | 2 cut(s) | 648, 784 |
| Bme1390I | CCNGG | 1 cut(s) | 609 |
| BmeT110I | CYCGRG | 1 cut(s) | 398 |
| BmgBI | CACGTC | 1 cut(s) | 605 |
| BmgT120I | GGNCC | 1 cut(s) | 673 |
| BmiI | GGNNCC | 1 cut(s) | 675 |
| BmrFI | CCNGG | 1 cut(s) | 609 |
| BmsI | GCATC | 2 cut(s) | 49, 185 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 512, 544 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 476, 506, 619, 649, 702, 732 |
| Bse1I | ACTGG | 1 cut(s) | 150 |
| Bse21I | CCTNAGG | 1 cut(s) | 678 |
| Bse3DI | GCAATG | 1 cut(s) | 373 |
| BseBI | CCWGG | 1 cut(s) | 609 |
| BseMI | GCAATG | 1 cut(s) | 373 |
| BseMII | CTCAG | 3 cut(s) | 69, 570, 692 |
| BseNI | ACTGG | 1 cut(s) | 150 |
| BseRI | GAGGAG | 1 cut(s) | 458 |
| BshFI | GGCC | 1 cut(s) | 674 |
| BsiHKCI | CYCGRG | 1 cut(s) | 398 |
| BsmAI | GTCTC | 3 cut(s) | 164, 200, 341 |
| BsnI | GGCC | 1 cut(s) | 674 |
| BsoBI | CYCGRG | 1 cut(s) | 398 |
| Bsp143I | GATC | 1 cut(s) | 770 |
| BspANI | GGCC | 1 cut(s) | 674 |
| BspCNI | CTCAG | 3 cut(s) | 70, 571, 691 |
| BspLI | GGNNCC | 1 cut(s) | 675 |
| BspQI | GCTCTTC | 1 cut(s) | 225 |
| BsrDI | GCAATG | 1 cut(s) | 373 |
| BsrI | ACTGG | 1 cut(s) | 150 |
| BssMI | GATC | 1 cut(s) | 770 |
| Bst2UI | CCWGG | 1 cut(s) | 609 |
| Bst6I | CTCTTC | 3 cut(s) | 225, 366, 471 |
| BstC8I | GCNNGC | 1 cut(s) | 701 |
| BstDEI | CTNAG | 5 cut(s) | 78, 236, 419, 579, 678 |
| BstHHI | GCGC | 1 cut(s) | 366 |
| BstKTI | GATC | 1 cut(s) | 773 |
| BstMAI | GTCTC | 3 cut(s) | 164, 200, 341 |
| BstMBI | GATC | 1 cut(s) | 770 |
| BstNI | CCWGG | 1 cut(s) | 609 |
| BstSCI | CCNGG | 1 cut(s) | 607 |
| Bsu36I | CCTNAGG | 1 cut(s) | 678 |
| BsuRI | GGCC | 1 cut(s) | 674 |
| BtrI | CACGTC | 1 cut(s) | 605 |
| BtsIMutI | CAGTG | 2 cut(s) | 157, 530 |
| Cac8I | GCNNGC | 1 cut(s) | 701 |
| CfoI | GCGC | 1 cut(s) | 366 |
| Cfr13I | GGNCC | 1 cut(s) | 673 |
| CviJI | RGCY | 7 cut(s) | 235, 298, 397, 527, 674, 688, 756 |
| CviKI_1 | RGCY | 7 cut(s) | 235, 298, 397, 527, 674, 688, 756 |
| DdeI | CTNAG | 5 cut(s) | 78, 236, 419, 579, 678 |
| DpnI | GATC | 1 cut(s) | 772 |
| DpnII | GATC | 1 cut(s) | 770 |
| Eam1104I | CTCTTC | 3 cut(s) | 225, 366, 471 |
| EarI | CTCTTC | 3 cut(s) | 225, 366, 471 |
| Eco57I | CTGAAG | 1 cut(s) | 200 |
| Eco81I | CCTNAGG | 1 cut(s) | 678 |
| Eco88I | CYCGRG | 1 cut(s) | 398 |
| EcoO109I | RGGNCCY | 1 cut(s) | 673 |
| EcoRII | CCWGG | 1 cut(s) | 607 |
| FaiI | YATR | 6 cut(s) | 161, 192, 491, 563, 588, 745 |
| FspBI | CTAG | 2 cut(s) | 648, 784 |
| GlaI | GCGC | 1 cut(s) | 365 |
| HaeIII | GGCC | 1 cut(s) | 674 |
| HhaI | GCGC | 1 cut(s) | 366 |
| Hin6I | GCGC | 1 cut(s) | 364 |
| HinP1I | GCGC | 1 cut(s) | 364 |
| HinfI | GANTC | 4 cut(s) | 74, 80, 590, 623 |
| HphI | GGTGA | 3 cut(s) | 175, 332, 623 |
| Hpy188I | TCNGA | 4 cut(s) | 33, 79, 219, 770 |
| Hpy188III | TCNNGA | 1 cut(s) | 680 |
| HpyAV | CCTTC | 2 cut(s) | 160, 475 |
| HpyCH4IV | ACGT | 1 cut(s) | 604 |
| HpyCH4V | TGCA | 3 cut(s) | 62, 176, 514 |
| HpyF3I | CTNAG | 5 cut(s) | 78, 236, 419, 579, 678 |
| HpySE526I | ACGT | 1 cut(s) | 604 |
| HspAI | GCGC | 1 cut(s) | 364 |
| Kzo9I | GATC | 1 cut(s) | 770 |
| LguI | GCTCTTC | 1 cut(s) | 225 |
| LpnPI | CCDG | 5 cut(s) | 163, 284, 594, 621, 665 |
| LweI | GCATC | 2 cut(s) | 49, 185 |
| MaeI | CTAG | 2 cut(s) | 648, 784 |
| MaeII | ACGT | 1 cut(s) | 604 |
| MaeIII | GTNAC | 3 cut(s) | 3, 320, 704 |
| MalI | GATC | 1 cut(s) | 772 |
| MboI | GATC | 1 cut(s) | 770 |
| MfeI | CAATTG | 1 cut(s) | 551 |
| MluCI | AATT | 7 cut(s) | 21, 200, 284, 383, 459, 516, 551 |
| MlyI | GAGTC | 3 cut(s) | 89, 599, 617 |
| MseI | TTAA | 3 cut(s) | 51, 330, 537 |
| MslI | CAYNNNNRTG | 1 cut(s) | 566 |
| MspR9I | CCNGG | 1 cut(s) | 609 |
| MunI | CAATTG | 1 cut(s) | 551 |
| MvaI | CCWGG | 1 cut(s) | 609 |
| NdeII | GATC | 1 cut(s) | 770 |
| NlaIV | GGNNCC | 1 cut(s) | 675 |
| NmuCI | GTSAC | 2 cut(s) | 3, 320 |
| PaeR7I | CTCGAG | 1 cut(s) | 398 |
| PciSI | GCTCTTC | 1 cut(s) | 225 |
| PfeI | GAWTC | 1 cut(s) | 74 |
| PleI | GAGTC | 3 cut(s) | 88, 598, 617 |
| PpsI | GAGTC | 3 cut(s) | 88, 598, 617 |
| Psp6I | CCWGG | 1 cut(s) | 607 |
| PspGI | CCWGG | 1 cut(s) | 607 |
| PspN4I | GGNNCC | 1 cut(s) | 675 |
| PspPI | GGNCC | 1 cut(s) | 673 |
| PspXI | VCTCGAGB | 1 cut(s) | 398 |
| RseI | CAYNNNNRTG | 1 cut(s) | 566 |
| SapI | GCTCTTC | 1 cut(s) | 225 |
| SaqAI | TTAA | 3 cut(s) | 51, 330, 537 |
| Sau3AI | GATC | 1 cut(s) | 770 |
| Sau96I | GGNCC | 1 cut(s) | 673 |
| SchI | GAGTC | 3 cut(s) | 89, 599, 617 |
| ScrFI | CCNGG | 1 cut(s) | 609 |
| SfaNI | GCATC | 2 cut(s) | 49, 185 |
| Sfr274I | CTCGAG | 1 cut(s) | 398 |
| SlaI | CTCGAG | 1 cut(s) | 398 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 566 |
| SmlI | CTYRAG | 1 cut(s) | 398 |
| SmoI | CTYRAG | 1 cut(s) | 398 |
| Sse9I | AATT | 7 cut(s) | 21, 200, 284, 383, 459, 516, 551 |
| SspMI | CTAG | 2 cut(s) | 648, 784 |
| StyD4I | CCNGG | 1 cut(s) | 607 |
| TaiI | ACGT | 1 cut(s) | 607 |
| TaqI | TCGA | 2 cut(s) | 179, 399 |
| TasI | AATT | 7 cut(s) | 21, 200, 284, 383, 459, 516, 551 |
| TfiI | GAWTC | 1 cut(s) | 74 |
| Tru1I | TTAA | 3 cut(s) | 51, 330, 537 |
| Tru9I | TTAA | 3 cut(s) | 51, 330, 537 |
| TscAI | CASTG | 2 cut(s) | 157, 537 |
| TseFI | GTSAC | 2 cut(s) | 3, 320 |
| Tsp45I | GTSAC | 2 cut(s) | 3, 320 |
| TspDTI | ATGAA | 2 cut(s) | 384, 396 |
| TspRI | CASTG | 2 cut(s) | 157, 537 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 157 |
| XhoI | CTCGAG | 1 cut(s) | 398 |
| XspI | CTAG | 2 cut(s) | 648, 784 |