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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 94 |
| AcsI | RAATTY | 2 cut(s) | 32, 417 |
| AfaI | GTAC | 1 cut(s) | 128 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 380 |
| AjiI | CACGTC | 1 cut(s) | 290 |
| AluBI | AGCT | 1 cut(s) | 413 |
| AluI | AGCT | 1 cut(s) | 413 |
| Alw26I | GTCTC | 1 cut(s) | 282 |
| Ama87I | CYCGRG | 1 cut(s) | 363 |
| AoxI | GGCC | 1 cut(s) | 357 |
| ApoI | RAATTY | 2 cut(s) | 32, 417 |
| AspLEI | GCGC | 1 cut(s) | 460 |
| AspS9I | GGNCC | 1 cut(s) | 358 |
| AsuC2I | CCSGG | 1 cut(s) | 294 |
| AsuHPI | GGTGA | 2 cut(s) | 35, 308 |
| AvaI | CYCGRG | 1 cut(s) | 363 |
| BccI | CCATC | 2 cut(s) | 20, 247 |
| BceAI | ACGGC | 1 cut(s) | 397 |
| BcnI | CCSGG | 1 cut(s) | 294 |
| BcoDI | GTCTC | 1 cut(s) | 282 |
| BfaI | CTAG | 2 cut(s) | 116, 333 |
| Bme1390I | CCNGG | 1 cut(s) | 294 |
| BmeT110I | CYCGRG | 1 cut(s) | 363 |
| BmgBI | CACGTC | 1 cut(s) | 290 |
| BmgT120I | GGNCC | 1 cut(s) | 358 |
| BmiI | GGNNCC | 1 cut(s) | 360 |
| BmrFI | CCNGG | 1 cut(s) | 294 |
| BmsI | GCATC | 1 cut(s) | 54 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 197, 229 |
| BpuEI | CTTGAG | 1 cut(s) | 393 |
| BpuMI | CCSGG | 1 cut(s) | 294 |
| BsaJI | CCNNGG | 1 cut(s) | 362 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 304, 334 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 380 |
| BseDI | CCNNGG | 1 cut(s) | 362 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 380 |
| BseRI | GAGGAG | 2 cut(s) | 306, 309 |
| BshFI | GGCC | 1 cut(s) | 359 |
| BsiHKCI | CYCGRG | 1 cut(s) | 363 |
| BsiSI | CCGG | 1 cut(s) | 293 |
| BslFI | GGGAC | 1 cut(s) | 380 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 380 |
| BsmAI | GTCTC | 1 cut(s) | 282 |
| BsmFI | GGGAC | 1 cut(s) | 380 |
| BsnI | GGCC | 1 cut(s) | 359 |
| BsoBI | CYCGRG | 1 cut(s) | 363 |
| Bsp1407I | TGTACA | 1 cut(s) | 126 |
| BspACI | CCGC | 1 cut(s) | 94 |
| BspANI | GGCC | 1 cut(s) | 359 |
| BspLI | GGNNCC | 1 cut(s) | 360 |
| BsrGI | TGTACA | 1 cut(s) | 126 |
| BssECI | CCNNGG | 1 cut(s) | 362 |
| Bst6I | CTCTTC | 1 cut(s) | 156 |
| BstAUI | TGTACA | 1 cut(s) | 126 |
| BstC8I | GCNNGC | 1 cut(s) | 386 |
| BstHHI | GCGC | 1 cut(s) | 460 |
| BstMAI | GTCTC | 1 cut(s) | 282 |
| BstSCI | CCNGG | 1 cut(s) | 292 |
| BsuRI | GGCC | 1 cut(s) | 359 |
| BtrI | CACGTC | 1 cut(s) | 290 |
| Cac8I | GCNNGC | 1 cut(s) | 386 |
| CfoI | GCGC | 1 cut(s) | 460 |
| Cfr13I | GGNCC | 1 cut(s) | 358 |
| Csp6I | GTAC | 1 cut(s) | 127 |
| CviJI | RGCY | 4 cut(s) | 212, 359, 373, 413 |
| CviKI_1 | RGCY | 4 cut(s) | 212, 359, 373, 413 |
| CviQI | GTAC | 1 cut(s) | 127 |
| Eam1104I | CTCTTC | 1 cut(s) | 156 |
| EarI | CTCTTC | 1 cut(s) | 156 |
| Eco88I | CYCGRG | 1 cut(s) | 363 |
| EcoO109I | RGGNCCY | 1 cut(s) | 358 |
| EcoRI | GAATTC | 1 cut(s) | 417 |
| FaiI | YATR | 4 cut(s) | 108, 248, 273, 462 |
| FaqI | GGGAC | 1 cut(s) | 380 |
| FspBI | CTAG | 2 cut(s) | 116, 333 |
| GlaI | GCGC | 1 cut(s) | 459 |
| HaeIII | GGCC | 1 cut(s) | 359 |
| HapII | CCGG | 1 cut(s) | 293 |
| HhaI | GCGC | 1 cut(s) | 460 |
| Hin6I | GCGC | 1 cut(s) | 458 |
| HinP1I | GCGC | 1 cut(s) | 458 |
| HinfI | GANTC | 2 cut(s) | 85, 275 |
| HpaII | CCGG | 1 cut(s) | 293 |
| HphI | GGTGA | 2 cut(s) | 35, 308 |
| Hpy166II | GTNNAC | 1 cut(s) | 127 |
| Hpy188I | TCNGA | 1 cut(s) | 423 |
| Hpy188III | TCNNGA | 2 cut(s) | 116, 365 |
| Hpy8I | GTNNAC | 1 cut(s) | 127 |
| Hpy99I | CGWCG | 1 cut(s) | 383 |
| HpyCH4IV | ACGT | 1 cut(s) | 289 |
| HpyCH4V | TGCA | 1 cut(s) | 67 |
| HpySE526I | ACGT | 1 cut(s) | 289 |
| HspAI | GCGC | 1 cut(s) | 458 |
| LpnPI | CCDG | 2 cut(s) | 306, 399 |
| LweI | GCATC | 1 cut(s) | 54 |
| MaeI | CTAG | 2 cut(s) | 116, 333 |
| MaeII | ACGT | 1 cut(s) | 289 |
| MboII | GAAGA | 5 cut(s) | 73, 85, 88, 131, 173 |
| MfeI | CAATTG | 1 cut(s) | 236 |
| MluCI | AATT | 4 cut(s) | 32, 145, 236, 417 |
| MlyI | GAGTC | 2 cut(s) | 94, 284 |
| MnlI | CCTC | 9 cut(s) | 63, 132, 184, 223, 235, 327, 330, 351, 372 |
| MseI | TTAA | 4 cut(s) | 56, 81, 179, 222 |
| MslI | CAYNNNNRTG | 1 cut(s) | 251 |
| MspI | CCGG | 1 cut(s) | 293 |
| MspR9I | CCNGG | 1 cut(s) | 294 |
| MunI | CAATTG | 1 cut(s) | 236 |
| NciI | CCSGG | 1 cut(s) | 294 |
| NlaIV | GGNNCC | 1 cut(s) | 360 |
| PleI | GAGTC | 2 cut(s) | 93, 283 |
| PpsI | GAGTC | 2 cut(s) | 93, 283 |
| PspN4I | GGNNCC | 1 cut(s) | 360 |
| PspPI | GGNCC | 1 cut(s) | 358 |
| RsaI | GTAC | 1 cut(s) | 128 |
| RsaNI | GTAC | 1 cut(s) | 127 |
| RseI | CAYNNNNRTG | 1 cut(s) | 251 |
| SaqAI | TTAA | 4 cut(s) | 56, 81, 179, 222 |
| Sau96I | GGNCC | 1 cut(s) | 358 |
| SchI | GAGTC | 2 cut(s) | 94, 284 |
| ScrFI | CCNGG | 1 cut(s) | 294 |
| SetI | ASST | 5 cut(s) | 11, 195, 292, 334, 415 |
| SfaNI | GCATC | 1 cut(s) | 54 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 251 |
| SmlI | CTYRAG | 1 cut(s) | 408 |
| SmoI | CTYRAG | 1 cut(s) | 408 |
| Sse9I | AATT | 4 cut(s) | 32, 145, 236, 417 |
| SsiI | CCGC | 1 cut(s) | 94 |
| SspI | AATATT | 1 cut(s) | 44 |
| SspMI | CTAG | 2 cut(s) | 116, 333 |
| StyD4I | CCNGG | 1 cut(s) | 292 |
| TaiI | ACGT | 1 cut(s) | 292 |
| TasI | AATT | 4 cut(s) | 32, 145, 236, 417 |
| TatI | WGTACW | 1 cut(s) | 126 |
| Tru1I | TTAA | 4 cut(s) | 56, 81, 179, 222 |
| Tru9I | TTAA | 4 cut(s) | 56, 81, 179, 222 |
| TspGWI | ACGGA | 1 cut(s) | 170 |
| XapI | RAATTY | 2 cut(s) | 32, 417 |
| XbaI | TCTAGA | 1 cut(s) | 115 |
| XspI | CTAG | 2 cut(s) | 116, 333 |