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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 13 |
| AgsI | TTSAA | 3 cut(s) | 206, 218, 446 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 101, 133 |
| AluBI | AGCT | 1 cut(s) | 170 |
| AluI | AGCT | 1 cut(s) | 170 |
| Alw26I | GTCTC | 1 cut(s) | 338 |
| AlwI | GGATC | 1 cut(s) | 13 |
| ApeKI | GCWGC | 2 cut(s) | 34, 41 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 213 |
| AsuHPI | GGTGA | 1 cut(s) | 323 |
| BanII | GRGCYC | 1 cut(s) | 128 |
| BbsI | GAAGAC | 1 cut(s) | 257 |
| BbvI | GCAGC | 2 cut(s) | 46, 53 |
| BccI | CCATC | 1 cut(s) | 106 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 7, 41 |
| BcoDI | GTCTC | 1 cut(s) | 338 |
| BfmI | CTRYAG | 2 cut(s) | 26, 198 |
| BisI | GCNGC | 2 cut(s) | 35, 42 |
| BlsI | GCNGC | 2 cut(s) | 36, 43 |
| BmsI | GCATC | 1 cut(s) | 53 |
| BoxI | GACNNNNGTC | 1 cut(s) | 177 |
| BpiI | GAAGAC | 1 cut(s) | 257 |
| BsaI | GGTCTC | 1 cut(s) | 338 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 101, 131 |
| Bse1I | ACTGG | 1 cut(s) | 401 |
| BseNI | ACTGG | 1 cut(s) | 401 |
| BseXI | GCAGC | 2 cut(s) | 46, 53 |
| BsgI | GTGCAG | 1 cut(s) | 49 |
| BsmAI | GTCTC | 1 cut(s) | 338 |
| Bso31I | GGTCTC | 1 cut(s) | 338 |
| Bsp1286I | GDGCHC | 1 cut(s) | 128 |
| Bsp143I | GATC | 1 cut(s) | 5 |
| BspMAI | CTGCAG | 1 cut(s) | 30 |
| BspPI | GGATC | 1 cut(s) | 13 |
| BspTNI | GGTCTC | 1 cut(s) | 338 |
| BsrI | ACTGG | 1 cut(s) | 401 |
| BssMI | GATC | 1 cut(s) | 5 |
| Bst4CI | ACNGT | 6 cut(s) | 15, 70, 117, 133, 148, 319 |
| BstC8I | GCNNGC | 1 cut(s) | 39 |
| BstKTI | GATC | 1 cut(s) | 8 |
| BstMAI | GTCTC | 1 cut(s) | 338 |
| BstMBI | GATC | 1 cut(s) | 5 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 34 |
| BstNSI | RCATGY | 1 cut(s) | 142 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 177 |
| BstSFI | CTRYAG | 2 cut(s) | 26, 198 |
| BstV1I | GCAGC | 2 cut(s) | 46, 53 |
| BstV2I | GAAGAC | 1 cut(s) | 257 |
| BstX2I | RGATCY | 1 cut(s) | 5 |
| BstYI | RGATCY | 1 cut(s) | 5 |
| BtsI | GCAGTG | 1 cut(s) | 61 |
| BtsIMutI | CAGTG | 3 cut(s) | 61, 75, 153 |
| Cac8I | GCNNGC | 1 cut(s) | 39 |
| CviAII | CATG | 1 cut(s) | 139 |
| CviJI | RGCY | 3 cut(s) | 126, 170, 267 |
| CviKI_1 | RGCY | 3 cut(s) | 126, 170, 267 |
| DpnI | GATC | 1 cut(s) | 7 |
| DpnII | GATC | 1 cut(s) | 5 |
| Eco24I | GRGCYC | 1 cut(s) | 128 |
| Eco31I | GGTCTC | 1 cut(s) | 338 |
| EcoT38I | GRGCYC | 1 cut(s) | 128 |
| FaeI | CATG | 1 cut(s) | 142 |
| FaiI | YATR | 4 cut(s) | 105, 140, 200, 236 |
| FatI | CATG | 1 cut(s) | 138 |
| Fnu4HI | GCNGC | 2 cut(s) | 35, 42 |
| FriOI | GRGCYC | 1 cut(s) | 128 |
| Fsp4HI | GCNGC | 2 cut(s) | 35, 42 |
| GluI | GCNGC | 2 cut(s) | 35, 42 |
| Hin1II | CATG | 1 cut(s) | 142 |
| HincII | GTYRAC | 1 cut(s) | 282 |
| HindII | GTYRAC | 1 cut(s) | 282 |
| HinfI | GANTC | 1 cut(s) | 110 |
| HpaI | GTTAAC | 1 cut(s) | 282 |
| HphI | GGTGA | 1 cut(s) | 323 |
| Hpy166II | GTNNAC | 2 cut(s) | 136, 282 |
| Hpy188I | TCNGA | 1 cut(s) | 183 |
| Hpy188III | TCNNGA | 1 cut(s) | 121 |
| Hpy8I | GTNNAC | 2 cut(s) | 136, 282 |
| HpyCH4III | ACNGT | 6 cut(s) | 15, 70, 117, 133, 148, 319 |
| HpyCH4IV | ACGT | 1 cut(s) | 345 |
| HpyCH4V | TGCA | 4 cut(s) | 11, 28, 66, 289 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 34 |
| HpySE526I | ACGT | 1 cut(s) | 345 |
| Hsp92II | CATG | 1 cut(s) | 142 |
| KspAI | GTTAAC | 1 cut(s) | 282 |
| Kzo9I | GATC | 1 cut(s) | 5 |
| LpnPI | CCDG | 5 cut(s) | 23, 134, 270, 294, 382 |
| Lsp1109I | GCAGC | 2 cut(s) | 46, 53 |
| LweI | GCATC | 1 cut(s) | 53 |
| MaeII | ACGT | 1 cut(s) | 345 |
| MaeIII | GTNAC | 1 cut(s) | 111 |
| MalI | GATC | 1 cut(s) | 7 |
| MboI | GATC | 1 cut(s) | 5 |
| MboII | GAAGA | 5 cut(s) | 89, 218, 259, 262, 439 |
| MflI | RGATCY | 1 cut(s) | 5 |
| MhlI | GDGCHC | 1 cut(s) | 128 |
| MlyI | GAGTC | 1 cut(s) | 119 |
| MnlI | CCTC | 2 cut(s) | 69, 105 |
| MroXI | GAANNNNTTC | 1 cut(s) | 213 |
| MseI | TTAA | 2 cut(s) | 281, 356 |
| MslI | CAYNNNNRTG | 1 cut(s) | 309 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 34 |
| NdeII | GATC | 1 cut(s) | 5 |
| NlaIII | CATG | 1 cut(s) | 142 |
| NmuCI | GTSAC | 1 cut(s) | 111 |
| NspI | RCATGY | 1 cut(s) | 142 |
| PdmI | GAANNNNTTC | 1 cut(s) | 213 |
| PkrI | GCNGC | 2 cut(s) | 36, 43 |
| PleI | GAGTC | 1 cut(s) | 118 |
| PpsI | GAGTC | 1 cut(s) | 118 |
| PshAI | GACNNNNGTC | 1 cut(s) | 177 |
| PstI | CTGCAG | 1 cut(s) | 30 |
| PsuI | RGATCY | 1 cut(s) | 5 |
| RseI | CAYNNNNRTG | 1 cut(s) | 309 |
| SaqAI | TTAA | 2 cut(s) | 281, 356 |
| SatI | GCNGC | 2 cut(s) | 35, 42 |
| Sau3AI | GATC | 1 cut(s) | 5 |
| SchI | GAGTC | 1 cut(s) | 119 |
| SduI | GDGCHC | 1 cut(s) | 128 |
| SetI | ASST | 6 cut(s) | 61, 97, 172, 294, 313, 348 |
| SfaNI | GCATC | 1 cut(s) | 53 |
| SfcI | CTRYAG | 2 cut(s) | 26, 198 |
| SgeI | CNNG | 8 cut(s) | 50, 133, 151, 179, 187, 269, 321, 409 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 309 |
| TaaI | ACNGT | 6 cut(s) | 15, 70, 117, 133, 148, 319 |
| TaiI | ACGT | 1 cut(s) | 348 |
| TaqI | TCGA | 2 cut(s) | 17, 89 |
| Tru1I | TTAA | 2 cut(s) | 281, 356 |
| Tru9I | TTAA | 2 cut(s) | 281, 356 |
| TscAI | CASTG | 3 cut(s) | 68, 75, 153 |
| TseFI | GTSAC | 1 cut(s) | 111 |
| TseI | GCWGC | 2 cut(s) | 34, 41 |
| Tsp45I | GTSAC | 1 cut(s) | 111 |
| TspRI | CASTG | 3 cut(s) | 68, 75, 153 |
| XceI | RCATGY | 1 cut(s) | 142 |
| XmnI | GAANNNNTTC | 1 cut(s) | 213 |