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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 507 |
| Acc36I | ACCTGC | 1 cut(s) | 196 |
| AccII | CGCG | 1 cut(s) | 401 |
| AciI | CCGC | 2 cut(s) | 77, 92 |
| AcsI | RAATTY | 1 cut(s) | 899 |
| AcyI | GRCGYC | 1 cut(s) | 504 |
| AfaI | GTAC | 3 cut(s) | 651, 869, 911 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 395 |
| AgsI | TTSAA | 2 cut(s) | 269, 839 |
| AjiI | CACGTC | 1 cut(s) | 38 |
| AjnI | CCWGG | 1 cut(s) | 40 |
| AluBI | AGCT | 3 cut(s) | 287, 551, 755 |
| AluI | AGCT | 3 cut(s) | 287, 551, 755 |
| AoxI | GGCC | 1 cut(s) | 105 |
| ApeKI | GCWGC | 3 cut(s) | 227, 284, 287 |
| ApoI | RAATTY | 1 cut(s) | 899 |
| AseI | ATTAAT | 1 cut(s) | 798 |
| AspLEI | GCGC | 2 cut(s) | 209, 401 |
| AspS9I | GGNCC | 1 cut(s) | 106 |
| AsuC2I | CCSGG | 1 cut(s) | 396 |
| AsuHPI | GGTGA | 4 cut(s) | 252, 294, 471, 697 |
| AsuII | TTCGAA | 1 cut(s) | 435 |
| AxyI | CCTNAGG | 2 cut(s) | 111, 315 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 446, 479 |
| BbvI | GCAGC | 3 cut(s) | 239, 274, 296 |
| BccI | CCATC | 1 cut(s) | 211 |
| BciT130I | CCWGG | 1 cut(s) | 42 |
| BcnI | CCSGG | 1 cut(s) | 396 |
| BfaI | CTAG | 1 cut(s) | 81 |
| BfmI | CTRYAG | 2 cut(s) | 225, 531 |
| BfuAI | ACCTGC | 1 cut(s) | 196 |
| BisI | GCNGC | 3 cut(s) | 228, 285, 288 |
| BlsI | GCNGC | 3 cut(s) | 229, 286, 289 |
| BmcAI | AGTACT | 1 cut(s) | 911 |
| Bme1390I | CCNGG | 2 cut(s) | 42, 396 |
| BmgBI | CACGTC | 1 cut(s) | 38 |
| BmgT120I | GGNCC | 1 cut(s) | 106 |
| BmiI | GGNNCC | 1 cut(s) | 108 |
| BmrFI | CCNGG | 2 cut(s) | 42, 396 |
| BmsI | GCATC | 3 cut(s) | 218, 230, 331 |
| BoxI | GACNNNNGTC | 1 cut(s) | 256 |
| Bpu14I | TTCGAA | 1 cut(s) | 435 |
| BpuEI | CTTGAG | 1 cut(s) | 141 |
| BpuMI | CCSGG | 1 cut(s) | 396 |
| BsaHI | GRCGYC | 1 cut(s) | 504 |
| BsaJI | CCNNGG | 1 cut(s) | 247 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 52, 82, 400, 430 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 395 |
| Bse1I | ACTGG | 2 cut(s) | 512, 917 |
| Bse21I | CCTNAGG | 2 cut(s) | 111, 315 |
| Bse3DI | GCAATG | 1 cut(s) | 52 |
| BseBI | CCWGG | 1 cut(s) | 42 |
| BseDI | CCNNGG | 1 cut(s) | 247 |
| BseGI | GGATG | 1 cut(s) | 811 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 395 |
| BseMI | GCAATG | 1 cut(s) | 52 |
| BseMII | CTCAG | 4 cut(s) | 125, 204, 306, 543 |
| BseNI | ACTGG | 2 cut(s) | 512, 917 |
| BseRI | GAGGAG | 3 cut(s) | 54, 57, 590 |
| BseXI | GCAGC | 3 cut(s) | 239, 274, 296 |
| BsgI | GTGCAG | 1 cut(s) | 273 |
| Bsh1236I | CGCG | 1 cut(s) | 401 |
| BshFI | GGCC | 1 cut(s) | 107 |
| BsiSI | CCGG | 1 cut(s) | 395 |
| BslFI | GGGAC | 1 cut(s) | 692 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 395 |
| BsmFI | GGGAC | 1 cut(s) | 692 |
| BsnI | GGCC | 1 cut(s) | 107 |
| Bsp119I | TTCGAA | 1 cut(s) | 435 |
| Bsp1407I | TGTACA | 1 cut(s) | 867 |
| Bsp143I | GATC | 1 cut(s) | 468 |
| BspACI | CCGC | 2 cut(s) | 77, 92 |
| BspANI | GGCC | 1 cut(s) | 107 |
| BspCNI | CTCAG | 4 cut(s) | 124, 205, 307, 544 |
| BspFNI | CGCG | 1 cut(s) | 401 |
| BspLI | GGNNCC | 1 cut(s) | 108 |
| BspMAI | CTGCAG | 1 cut(s) | 229 |
| BspMI | ACCTGC | 1 cut(s) | 196 |
| BspT104I | TTCGAA | 1 cut(s) | 435 |
| BsrDI | GCAATG | 1 cut(s) | 52 |
| BsrGI | TGTACA | 1 cut(s) | 867 |
| BsrI | ACTGG | 2 cut(s) | 512, 917 |
| BssECI | CCNNGG | 1 cut(s) | 247 |
| BssMI | GATC | 1 cut(s) | 468 |
| BssNI | GRCGYC | 1 cut(s) | 504 |
| BssT1I | CCWWGG | 1 cut(s) | 247 |
| Bst2UI | CCWGG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 1 cut(s) | 867 |
| Bst6I | CTCTTC | 1 cut(s) | 629 |
| BstACI | GRCGYC | 1 cut(s) | 504 |
| BstAUI | TGTACA | 1 cut(s) | 867 |
| BstBI | TTCGAA | 1 cut(s) | 435 |
| BstC8I | GCNNGC | 5 cut(s) | 134, 193, 205, 346, 447 |
| BstDEI | CTNAG | 5 cut(s) | 111, 213, 315, 552, 595 |
| BstF5I | GGATG | 1 cut(s) | 811 |
| BstFNI | CGCG | 1 cut(s) | 401 |
| BstHHI | GCGC | 2 cut(s) | 209, 401 |
| BstKTI | GATC | 1 cut(s) | 471 |
| BstMBI | GATC | 1 cut(s) | 468 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 227, 284, 451 |
| BstNI | CCWGG | 1 cut(s) | 42 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 256 |
| BstSCI | CCNGG | 2 cut(s) | 40, 394 |
| BstSFI | CTRYAG | 2 cut(s) | 225, 531 |
| BstUI | CGCG | 1 cut(s) | 401 |
| BstV1I | GCAGC | 3 cut(s) | 239, 274, 296 |
| Bsu36I | CCTNAGG | 2 cut(s) | 111, 315 |
| BsuRI | GGCC | 1 cut(s) | 107 |
| BtrI | CACGTC | 1 cut(s) | 38 |
| BtsCI | GGATG | 1 cut(s) | 811 |
| BveI | ACCTGC | 1 cut(s) | 196 |
| Cac8I | GCNNGC | 5 cut(s) | 134, 193, 205, 346, 447 |
| CfoI | GCGC | 2 cut(s) | 209, 401 |
| Cfr13I | GGNCC | 1 cut(s) | 106 |
| Csp6I | GTAC | 3 cut(s) | 650, 868, 910 |
| CviAII | CATG | 1 cut(s) | 785 |
| CviQI | GTAC | 3 cut(s) | 650, 868, 910 |
| DdeI | CTNAG | 5 cut(s) | 111, 213, 315, 552, 595 |
| DpnI | GATC | 1 cut(s) | 470 |
| DpnII | GATC | 1 cut(s) | 468 |
| Eam1104I | CTCTTC | 1 cut(s) | 629 |
| EarI | CTCTTC | 1 cut(s) | 629 |
| EciI | GGCGGA | 1 cut(s) | 81 |
| Eco130I | CCWWGG | 1 cut(s) | 247 |
| Eco32I | GATATC | 1 cut(s) | 582 |
| Eco81I | CCTNAGG | 2 cut(s) | 111, 315 |
| EcoO109I | RGGNCCY | 1 cut(s) | 106 |
| EcoRII | CCWGG | 1 cut(s) | 40 |
| EcoRV | GATATC | 1 cut(s) | 582 |
| EcoT14I | CCWWGG | 1 cut(s) | 247 |
| EcoT22I | ATGCAT | 1 cut(s) | 376 |
| ErhI | CCWWGG | 1 cut(s) | 247 |
| FaeI | CATG | 1 cut(s) | 788 |
| FaiI | YATR | 5 cut(s) | 443, 476, 621, 666, 786 |
| FaqI | GGGAC | 1 cut(s) | 692 |
| FatI | CATG | 1 cut(s) | 784 |
| Fnu4HI | GCNGC | 3 cut(s) | 228, 285, 288 |
| FokI | GGATG | 1 cut(s) | 818 |
| Fsp4HI | GCNGC | 3 cut(s) | 228, 285, 288 |
| FspBI | CTAG | 1 cut(s) | 81 |
| GlaI | GCGC | 2 cut(s) | 208, 400 |
| GluI | GCNGC | 3 cut(s) | 228, 285, 288 |
| HaeIII | GGCC | 1 cut(s) | 107 |
| HapII | CCGG | 1 cut(s) | 395 |
| HhaI | GCGC | 2 cut(s) | 209, 401 |
| Hin1I | GRCGYC | 1 cut(s) | 504 |
| Hin1II | CATG | 1 cut(s) | 788 |
| Hin6I | GCGC | 2 cut(s) | 207, 399 |
| HinP1I | GCGC | 2 cut(s) | 207, 399 |
| HinfI | GANTC | 5 cut(s) | 23, 56, 295, 599, 720 |
| HpaII | CCGG | 1 cut(s) | 395 |
| HphI | GGTGA | 4 cut(s) | 252, 294, 471, 697 |
| Hpy188I | TCNGA | 2 cut(s) | 214, 468 |
| Hpy188III | TCNNGA | 2 cut(s) | 113, 314 |
| Hpy99I | CGWCG | 1 cut(s) | 509 |
| HpyCH4III | ACNGT | 1 cut(s) | 867 |
| HpyCH4IV | ACGT | 2 cut(s) | 37, 504 |
| HpyCH4V | TGCA | 5 cut(s) | 227, 290, 348, 374, 445 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 227, 284, 451 |
| HpyF3I | CTNAG | 5 cut(s) | 111, 213, 315, 552, 595 |
| HpySE526I | ACGT | 2 cut(s) | 37, 504 |
| Hsp92I | GRCGYC | 1 cut(s) | 504 |
| Hsp92II | CATG | 1 cut(s) | 788 |
| HspAI | GCGC | 2 cut(s) | 207, 399 |
| Kzo9I | GATC | 1 cut(s) | 468 |
| LmnI | GCTCC | 1 cut(s) | 200 |
| Lsp1109I | GCAGC | 3 cut(s) | 239, 274, 296 |
| LweI | GCATC | 3 cut(s) | 218, 230, 331 |
| MaeI | CTAG | 1 cut(s) | 81 |
| MaeII | ACGT | 2 cut(s) | 37, 504 |
| MaeIII | GTNAC | 2 cut(s) | 258, 402 |
| MalI | GATC | 1 cut(s) | 470 |
| MboI | GATC | 1 cut(s) | 468 |
| MboII | GAAGA | 3 cut(s) | 350, 449, 646 |
| MluCI | AATT | 5 cut(s) | 323, 517, 567, 834, 899 |
| MlyI | GAGTC | 2 cut(s) | 32, 50 |
| Mph1103I | ATGCAT | 1 cut(s) | 376 |
| MseI | TTAA | 3 cut(s) | 321, 369, 798 |
| MslI | CAYNNNNRTG | 1 cut(s) | 268 |
| MspA1I | CMGCKG | 2 cut(s) | 287, 755 |
| MspI | CCGG | 1 cut(s) | 395 |
| MspR9I | CCNGG | 2 cut(s) | 42, 396 |
| MvaI | CCWGG | 1 cut(s) | 42 |
| MvnI | CGCG | 1 cut(s) | 401 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 227, 284, 451 |
| NciI | CCSGG | 1 cut(s) | 396 |
| NdeII | GATC | 1 cut(s) | 468 |
| NlaIII | CATG | 1 cut(s) | 788 |
| NlaIV | GGNNCC | 1 cut(s) | 108 |
| NmuCI | GTSAC | 1 cut(s) | 258 |
| NsiI | ATGCAT | 1 cut(s) | 376 |
| NspV | TTCGAA | 1 cut(s) | 435 |
| PfeI | GAWTC | 3 cut(s) | 295, 599, 720 |
| PkrI | GCNGC | 3 cut(s) | 229, 286, 289 |
| PleI | GAGTC | 2 cut(s) | 31, 50 |
| PpsI | GAGTC | 2 cut(s) | 31, 50 |
| PshAI | GACNNNNGTC | 1 cut(s) | 256 |
| PshBI | ATTAAT | 1 cut(s) | 798 |
| Psp6I | CCWGG | 1 cut(s) | 40 |
| PspGI | CCWGG | 1 cut(s) | 40 |
| PspN4I | GGNNCC | 1 cut(s) | 108 |
| PspPI | GGNCC | 1 cut(s) | 106 |
| PstI | CTGCAG | 1 cut(s) | 229 |
| PvuII | CAGCTG | 2 cut(s) | 287, 755 |
| RsaI | GTAC | 3 cut(s) | 651, 869, 911 |
| RsaNI | GTAC | 3 cut(s) | 650, 868, 910 |
| RseI | CAYNNNNRTG | 1 cut(s) | 268 |
| SaqAI | TTAA | 3 cut(s) | 321, 369, 798 |
| SatI | GCNGC | 3 cut(s) | 228, 285, 288 |
| Sau3AI | GATC | 1 cut(s) | 468 |
| Sau96I | GGNCC | 1 cut(s) | 106 |
| ScaI | AGTACT | 1 cut(s) | 911 |
| SchI | GAGTC | 2 cut(s) | 32, 50 |
| ScrFI | CCNGG | 2 cut(s) | 42, 396 |
| SfaNI | GCATC | 3 cut(s) | 218, 230, 331 |
| SfcI | CTRYAG | 2 cut(s) | 225, 531 |
| SfuI | TTCGAA | 1 cut(s) | 435 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 268 |
| SmlI | CTYRAG | 1 cut(s) | 156 |
| SmoI | CTYRAG | 1 cut(s) | 156 |
| Sse9I | AATT | 5 cut(s) | 323, 517, 567, 834, 899 |
| SsiI | CCGC | 2 cut(s) | 77, 92 |
| SspMI | CTAG | 1 cut(s) | 81 |
| StyD4I | CCNGG | 2 cut(s) | 40, 394 |
| StyI | CCWWGG | 1 cut(s) | 247 |
| TaaI | ACNGT | 1 cut(s) | 867 |
| TaiI | ACGT | 2 cut(s) | 40, 507 |
| TaqI | TCGA | 2 cut(s) | 435, 507 |
| TasI | AATT | 5 cut(s) | 323, 517, 567, 834, 899 |
| TatI | WGTACW | 3 cut(s) | 649, 867, 909 |
| TfiI | GAWTC | 3 cut(s) | 295, 599, 720 |
| Tru1I | TTAA | 3 cut(s) | 321, 369, 798 |
| Tru9I | TTAA | 3 cut(s) | 321, 369, 798 |
| TseFI | GTSAC | 1 cut(s) | 258 |
| TseI | GCWGC | 3 cut(s) | 227, 284, 287 |
| Tsp45I | GTSAC | 1 cut(s) | 258 |
| TspDTI | ATGAA | 2 cut(s) | 287, 547 |
| TspGWI | ACGGA | 1 cut(s) | 350 |
| VspI | ATTAAT | 1 cut(s) | 798 |
| XapI | RAATTY | 1 cut(s) | 899 |
| XspI | CTAG | 1 cut(s) | 81 |
| ZraI | GACGTC | 1 cut(s) | 505 |
| ZrmI | AGTACT | 1 cut(s) | 911 |
| Zsp2I | ATGCAT | 1 cut(s) | 376 |