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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 774, 782 |
| AclWI | GGATC | 1 cut(s) | 784 |
| AcsI | RAATTY | 1 cut(s) | 732 |
| AcuI | CTGAAG | 1 cut(s) | 200 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 950 |
| AgsI | TTSAA | 3 cut(s) | 140, 430, 824 |
| AjiI | CACGTC | 1 cut(s) | 605 |
| AjnI | CCWGG | 1 cut(s) | 607 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 248, 280, 444, 476 |
| AluBI | AGCT | 4 cut(s) | 235, 397, 418, 842 |
| AluI | AGCT | 4 cut(s) | 235, 397, 418, 842 |
| Alw26I | GTCTC | 2 cut(s) | 164, 341 |
| AlwI | GGATC | 1 cut(s) | 784 |
| AoxI | GGCC | 1 cut(s) | 672 |
| ApeKI | GCWGC | 1 cut(s) | 842 |
| ApoI | RAATTY | 1 cut(s) | 732 |
| AspLEI | GCGC | 1 cut(s) | 956 |
| AspS9I | GGNCC | 1 cut(s) | 673 |
| AsuHPI | GGTGA | 5 cut(s) | 175, 332, 623, 807, 849 |
| AxyI | CCTNAGG | 1 cut(s) | 678 |
| BbvI | GCAGC | 1 cut(s) | 829 |
| BccI | CCATC | 2 cut(s) | 113, 337 |
| BceAI | ACGGC | 1 cut(s) | 783 |
| BciT130I | CCWGG | 1 cut(s) | 609 |
| BcoDI | GTCTC | 2 cut(s) | 164, 341 |
| BfaI | CTAG | 2 cut(s) | 648, 756 |
| BfoI | RGCGCY | 1 cut(s) | 957 |
| BisI | GCNGC | 1 cut(s) | 843 |
| BlsI | GCNGC | 1 cut(s) | 844 |
| Bme1390I | CCNGG | 1 cut(s) | 609 |
| BmgBI | CACGTC | 1 cut(s) | 605 |
| BmgT120I | GGNCC | 1 cut(s) | 673 |
| BmiI | GGNNCC | 2 cut(s) | 675, 786 |
| BmrFI | CCNGG | 1 cut(s) | 609 |
| BmsI | GCATC | 3 cut(s) | 49, 185, 886 |
| BoxI | GACNNNNGTC | 1 cut(s) | 811 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 512, 544 |
| BsaJI | CCNNGG | 1 cut(s) | 765 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 281, 311, 476, 506, 619, 649, 955, 985 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 950 |
| Bse1I | ACTGG | 2 cut(s) | 150, 985 |
| Bse21I | CCTNAGG | 1 cut(s) | 678 |
| Bse3DI | GCAATG | 1 cut(s) | 373 |
| BseBI | CCWGG | 1 cut(s) | 609 |
| BseDI | CCNNGG | 1 cut(s) | 765 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 950 |
| BseMI | GCAATG | 1 cut(s) | 373 |
| BseMII | CTCAG | 4 cut(s) | 69, 227, 570, 692 |
| BseNI | ACTGG | 2 cut(s) | 150, 985 |
| BseRI | GAGGAG | 1 cut(s) | 458 |
| BseXI | GCAGC | 1 cut(s) | 829 |
| BsgI | GTGCAG | 1 cut(s) | 828 |
| BshFI | GGCC | 1 cut(s) | 674 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 950 |
| BsmAI | GTCTC | 2 cut(s) | 164, 341 |
| BsmI | GAATGC | 1 cut(s) | 830 |
| BsnI | GGCC | 1 cut(s) | 674 |
| Bsp143I | GATC | 1 cut(s) | 776 |
| BspACI | CCGC | 2 cut(s) | 774, 782 |
| BspANI | GGCC | 1 cut(s) | 674 |
| BspCNI | CTCAG | 4 cut(s) | 70, 228, 571, 691 |
| BspLI | GGNNCC | 2 cut(s) | 675, 786 |
| BspPI | GGATC | 1 cut(s) | 784 |
| BspQI | GCTCTTC | 2 cut(s) | 225, 408 |
| BsrDI | GCAATG | 1 cut(s) | 373 |
| BsrI | ACTGG | 2 cut(s) | 150, 985 |
| BssECI | CCNNGG | 1 cut(s) | 765 |
| BssMI | GATC | 1 cut(s) | 776 |
| Bst2UI | CCWGG | 1 cut(s) | 609 |
| Bst6I | CTCTTC | 4 cut(s) | 225, 366, 408, 471 |
| BstC8I | GCNNGC | 3 cut(s) | 548, 772, 901 |
| BstDEI | CTNAG | 5 cut(s) | 78, 236, 419, 579, 678 |
| BstDSI | CCRYGG | 1 cut(s) | 765 |
| BstH2I | RGCGCY | 1 cut(s) | 957 |
| BstHHI | GCGC | 1 cut(s) | 956 |
| BstKTI | GATC | 1 cut(s) | 779 |
| BstMAI | GTCTC | 2 cut(s) | 164, 341 |
| BstMBI | GATC | 1 cut(s) | 776 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 839 |
| BstNI | CCWGG | 1 cut(s) | 609 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 811 |
| BstSCI | CCNGG | 1 cut(s) | 607 |
| BstV1I | GCAGC | 1 cut(s) | 829 |
| BstX2I | RGATCY | 1 cut(s) | 776 |
| BstYI | RGATCY | 1 cut(s) | 776 |
| Bsu36I | CCTNAGG | 1 cut(s) | 678 |
| BsuRI | GGCC | 1 cut(s) | 674 |
| BtgI | CCRYGG | 1 cut(s) | 765 |
| BtrI | CACGTC | 1 cut(s) | 605 |
| BtsI | GCAGTG | 1 cut(s) | 5 |
| BtsIMutI | CAGTG | 3 cut(s) | 5, 157, 530 |
| Cac8I | GCNNGC | 3 cut(s) | 548, 772, 901 |
| CfoI | GCGC | 1 cut(s) | 956 |
| Cfr13I | GGNCC | 1 cut(s) | 673 |
| CviJI | RGCY | 7 cut(s) | 235, 397, 418, 527, 674, 842, 1004 |
| CviKI_1 | RGCY | 7 cut(s) | 235, 397, 418, 527, 674, 842, 1004 |
| DdeI | CTNAG | 5 cut(s) | 78, 236, 419, 579, 678 |
| DpnI | GATC | 1 cut(s) | 778 |
| DpnII | GATC | 1 cut(s) | 776 |
| Eam1104I | CTCTTC | 4 cut(s) | 225, 366, 408, 471 |
| EarI | CTCTTC | 4 cut(s) | 225, 366, 408, 471 |
| Eco57I | CTGAAG | 1 cut(s) | 200 |
| Eco81I | CCTNAGG | 1 cut(s) | 678 |
| EcoO109I | RGGNCCY | 1 cut(s) | 673 |
| EcoRI | GAATTC | 1 cut(s) | 732 |
| EcoRII | CCWGG | 1 cut(s) | 607 |
| EcoT22I | ATGCAT | 1 cut(s) | 931 |
| FaiI | YATR | 5 cut(s) | 161, 491, 563, 588, 998 |
| Fnu4HI | GCNGC | 1 cut(s) | 843 |
| Fsp4HI | GCNGC | 1 cut(s) | 843 |
| FspBI | CTAG | 2 cut(s) | 648, 756 |
| GlaI | GCGC | 1 cut(s) | 955 |
| GluI | GCNGC | 1 cut(s) | 843 |
| HaeII | RGCGCY | 1 cut(s) | 957 |
| HaeIII | GGCC | 1 cut(s) | 674 |
| HhaI | GCGC | 1 cut(s) | 956 |
| Hin6I | GCGC | 1 cut(s) | 954 |
| HinP1I | GCGC | 1 cut(s) | 954 |
| HinfI | GANTC | 6 cut(s) | 74, 80, 206, 590, 623, 761 |
| HphI | GGTGA | 5 cut(s) | 175, 332, 623, 807, 849 |
| Hpy188I | TCNGA | 4 cut(s) | 33, 79, 219, 724 |
| Hpy188III | TCNNGA | 1 cut(s) | 680 |
| HpyAV | CCTTC | 4 cut(s) | 160, 395, 475, 957 |
| HpyCH4IV | ACGT | 1 cut(s) | 604 |
| HpyCH4V | TGCA | 6 cut(s) | 62, 176, 514, 845, 903, 929 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 839 |
| HpyF3I | CTNAG | 5 cut(s) | 78, 236, 419, 579, 678 |
| HpySE526I | ACGT | 1 cut(s) | 604 |
| HspAI | GCGC | 1 cut(s) | 954 |
| Kzo9I | GATC | 1 cut(s) | 776 |
| LguI | GCTCTTC | 2 cut(s) | 225, 408 |
| LpnPI | CCDG | 8 cut(s) | 163, 594, 621, 665, 682, 722, 882, 998 |
| Lsp1109I | GCAGC | 1 cut(s) | 829 |
| LweI | GCATC | 3 cut(s) | 49, 185, 886 |
| MaeI | CTAG | 2 cut(s) | 648, 756 |
| MaeII | ACGT | 1 cut(s) | 604 |
| MaeIII | GTNAC | 3 cut(s) | 3, 320, 813 |
| MalI | GATC | 1 cut(s) | 778 |
| MboI | GATC | 1 cut(s) | 776 |
| MfeI | CAATTG | 1 cut(s) | 551 |
| MflI | RGATCY | 1 cut(s) | 776 |
| MluCI | AATT | 9 cut(s) | 21, 200, 284, 383, 459, 516, 551, 732, 878 |
| MlyI | GAGTC | 5 cut(s) | 89, 215, 599, 617, 755 |
| MmeI | TCCRAC | 2 cut(s) | 279, 969 |
| Mph1103I | ATGCAT | 1 cut(s) | 931 |
| MseI | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| MslI | CAYNNNNRTG | 2 cut(s) | 566, 823 |
| MspR9I | CCNGG | 1 cut(s) | 609 |
| MunI | CAATTG | 1 cut(s) | 551 |
| Mva1269I | GAATGC | 1 cut(s) | 830 |
| MvaI | CCWGG | 1 cut(s) | 609 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 839 |
| NdeII | GATC | 1 cut(s) | 776 |
| NlaIV | GGNNCC | 2 cut(s) | 675, 786 |
| NmuCI | GTSAC | 3 cut(s) | 3, 320, 813 |
| NsiI | ATGCAT | 1 cut(s) | 931 |
| PciSI | GCTCTTC | 2 cut(s) | 225, 408 |
| PctI | GAATGC | 1 cut(s) | 830 |
| PfeI | GAWTC | 1 cut(s) | 74 |
| PkrI | GCNGC | 1 cut(s) | 844 |
| PleI | GAGTC | 5 cut(s) | 88, 214, 598, 617, 755 |
| PpsI | GAGTC | 5 cut(s) | 88, 214, 598, 617, 755 |
| PshAI | GACNNNNGTC | 1 cut(s) | 811 |
| Psp6I | CCWGG | 1 cut(s) | 607 |
| PspGI | CCWGG | 1 cut(s) | 607 |
| PspN4I | GGNNCC | 2 cut(s) | 675, 786 |
| PspPI | GGNCC | 1 cut(s) | 673 |
| PsuI | RGATCY | 1 cut(s) | 776 |
| RseI | CAYNNNNRTG | 2 cut(s) | 566, 823 |
| SapI | GCTCTTC | 2 cut(s) | 225, 408 |
| SaqAI | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| SatI | GCNGC | 1 cut(s) | 843 |
| Sau3AI | GATC | 1 cut(s) | 776 |
| Sau96I | GGNCC | 1 cut(s) | 673 |
| SchI | GAGTC | 5 cut(s) | 89, 215, 599, 617, 755 |
| ScrFI | CCNGG | 1 cut(s) | 609 |
| SfaNI | GCATC | 3 cut(s) | 49, 185, 886 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 566, 823 |
| Sse9I | AATT | 9 cut(s) | 21, 200, 284, 383, 459, 516, 551, 732, 878 |
| SsiI | CCGC | 2 cut(s) | 774, 782 |
| SspMI | CTAG | 2 cut(s) | 648, 756 |
| StyD4I | CCNGG | 1 cut(s) | 607 |
| TaiI | ACGT | 1 cut(s) | 607 |
| TaqI | TCGA | 1 cut(s) | 399 |
| TasI | AATT | 9 cut(s) | 21, 200, 284, 383, 459, 516, 551, 732, 878 |
| TfiI | GAWTC | 1 cut(s) | 74 |
| Tru1I | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| Tru9I | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| TscAI | CASTG | 3 cut(s) | 12, 157, 537 |
| TseFI | GTSAC | 3 cut(s) | 3, 320, 813 |
| TseI | GCWGC | 1 cut(s) | 842 |
| Tsp45I | GTSAC | 3 cut(s) | 3, 320, 813 |
| TspDTI | ATGAA | 3 cut(s) | 384, 396, 842 |
| TspRI | CASTG | 3 cut(s) | 12, 157, 537 |
| XapI | RAATTY | 1 cut(s) | 732 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 157 |
| XspI | CTAG | 2 cut(s) | 648, 756 |
| Zsp2I | ATGCAT | 1 cut(s) | 931 |