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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 70 |
| AciI | CCGC | 1 cut(s) | 431 |
| AcsI | RAATTY | 1 cut(s) | 334 |
| AcuI | CTGAAG | 3 cut(s) | 23, 144, 173 |
| AgsI | TTSAA | 2 cut(s) | 241, 379 |
| AluBI | AGCT | 6 cut(s) | 92, 158, 187, 250, 428, 441 |
| AluI | AGCT | 6 cut(s) | 92, 158, 187, 250, 428, 441 |
| Aor13HI | TCCGGA | 1 cut(s) | 70 |
| ApeKI | GCWGC | 2 cut(s) | 425, 433 |
| ApoI | RAATTY | 1 cut(s) | 334 |
| AsuHPI | GGTGA | 1 cut(s) | 367 |
| BanII | GRGCYC | 1 cut(s) | 404 |
| BbvI | GCAGC | 2 cut(s) | 437, 445 |
| BfaI | CTAG | 1 cut(s) | 128 |
| BfmI | CTRYAG | 1 cut(s) | 222 |
| BglII | AGATCT | 1 cut(s) | 212 |
| BisI | GCNGC | 2 cut(s) | 426, 434 |
| BlsI | GCNGC | 2 cut(s) | 427, 435 |
| BmsI | GCATC | 1 cut(s) | 356 |
| BpmI | CTGGAG | 1 cut(s) | 323 |
| BsaWI | WCCGGW | 1 cut(s) | 70 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 380, 410, 412, 442 |
| BseAI | TCCGGA | 1 cut(s) | 70 |
| BseRI | GAGGAG | 3 cut(s) | 53, 215, 218 |
| BseXI | GCAGC | 2 cut(s) | 437, 445 |
| BsiSI | CCGG | 1 cut(s) | 71 |
| Bsp1286I | GDGCHC | 1 cut(s) | 404 |
| Bsp13I | TCCGGA | 1 cut(s) | 70 |
| Bsp143I | GATC | 3 cut(s) | 47, 97, 212 |
| BspACI | CCGC | 1 cut(s) | 431 |
| BspEI | TCCGGA | 1 cut(s) | 70 |
| BspMAI | CTGCAG | 1 cut(s) | 226 |
| BssMI | GATC | 3 cut(s) | 47, 97, 212 |
| Bst4CI | ACNGT | 1 cut(s) | 67 |
| Bst6I | CTCTTC | 2 cut(s) | 30, 72 |
| BstDEI | CTNAG | 1 cut(s) | 272 |
| BstKTI | GATC | 3 cut(s) | 50, 100, 215 |
| BstMBI | GATC | 3 cut(s) | 47, 97, 212 |
| BstSFI | CTRYAG | 1 cut(s) | 222 |
| BstV1I | GCAGC | 2 cut(s) | 437, 445 |
| BstX2I | RGATCY | 1 cut(s) | 212 |
| BstYI | RGATCY | 1 cut(s) | 212 |
| CviAII | CATG | 1 cut(s) | 149 |
| CviJI | RGCY | 8 cut(s) | 92, 143, 158, 187, 250, 402, 428, 441 |
| CviKI_1 | RGCY | 8 cut(s) | 92, 143, 158, 187, 250, 402, 428, 441 |
| DdeI | CTNAG | 1 cut(s) | 272 |
| DpnI | GATC | 3 cut(s) | 49, 99, 214 |
| DpnII | GATC | 3 cut(s) | 47, 97, 212 |
| Eam1104I | CTCTTC | 2 cut(s) | 30, 72 |
| EarI | CTCTTC | 2 cut(s) | 30, 72 |
| Eco24I | GRGCYC | 1 cut(s) | 404 |
| Eco57I | CTGAAG | 3 cut(s) | 23, 144, 173 |
| EcoT38I | GRGCYC | 1 cut(s) | 404 |
| FaeI | CATG | 1 cut(s) | 152 |
| FaiI | YATR | 3 cut(s) | 150, 218, 293 |
| FatI | CATG | 1 cut(s) | 148 |
| Fnu4HI | GCNGC | 2 cut(s) | 426, 434 |
| FriOI | GRGCYC | 1 cut(s) | 404 |
| Fsp4HI | GCNGC | 2 cut(s) | 426, 434 |
| FspBI | CTAG | 1 cut(s) | 128 |
| GluI | GCNGC | 2 cut(s) | 426, 434 |
| GsuI | CTGGAG | 1 cut(s) | 323 |
| HapII | CCGG | 1 cut(s) | 71 |
| Hin1II | CATG | 1 cut(s) | 152 |
| HinfI | GANTC | 5 cut(s) | 20, 26, 298, 362, 409 |
| HpaII | CCGG | 1 cut(s) | 71 |
| HphI | GGTGA | 1 cut(s) | 367 |
| Hpy188I | TCNGA | 7 cut(s) | 25, 31, 52, 102, 163, 192, 367 |
| Hpy188III | TCNNGA | 2 cut(s) | 71, 302 |
| HpyAV | CCTTC | 3 cut(s) | 352, 373, 458 |
| HpyCH4III | ACNGT | 1 cut(s) | 67 |
| HpyCH4V | TGCA | 2 cut(s) | 224, 347 |
| HpyF3I | CTNAG | 1 cut(s) | 272 |
| Hsp92II | CATG | 1 cut(s) | 152 |
| Kpn2I | TCCGGA | 1 cut(s) | 70 |
| Kzo9I | GATC | 3 cut(s) | 47, 97, 212 |
| LmnI | GCTCC | 4 cut(s) | 89, 388, 433, 446 |
| LpnPI | CCDG | 3 cut(s) | 84, 251, 287 |
| Lsp1109I | GCAGC | 2 cut(s) | 437, 445 |
| LweI | GCATC | 1 cut(s) | 356 |
| MaeI | CTAG | 1 cut(s) | 128 |
| MaeIII | GTNAC | 1 cut(s) | 325 |
| MalI | GATC | 3 cut(s) | 49, 99, 214 |
| MboI | GATC | 3 cut(s) | 47, 97, 212 |
| MboII | GAAGA | 7 cut(s) | 47, 86, 89, 128, 131, 253, 342 |
| MflI | RGATCY | 1 cut(s) | 212 |
| MhlI | GDGCHC | 1 cut(s) | 404 |
| MluCI | AATT | 5 cut(s) | 103, 235, 334, 374, 475 |
| MlyI | GAGTC | 2 cut(s) | 371, 403 |
| MroI | TCCGGA | 1 cut(s) | 70 |
| MseI | TTAA | 1 cut(s) | 478 |
| MspI | CCGG | 1 cut(s) | 71 |
| NdeII | GATC | 3 cut(s) | 47, 97, 212 |
| NlaIII | CATG | 1 cut(s) | 152 |
| NmuCI | GTSAC | 1 cut(s) | 325 |
| PfeI | GAWTC | 3 cut(s) | 20, 26, 298 |
| PkrI | GCNGC | 2 cut(s) | 427, 435 |
| PleI | GAGTC | 2 cut(s) | 370, 403 |
| PpsI | GAGTC | 2 cut(s) | 370, 403 |
| PstI | CTGCAG | 1 cut(s) | 226 |
| PsuI | RGATCY | 1 cut(s) | 212 |
| SaqAI | TTAA | 1 cut(s) | 478 |
| SatI | GCNGC | 2 cut(s) | 426, 434 |
| Sau3AI | GATC | 3 cut(s) | 47, 97, 212 |
| SchI | GAGTC | 2 cut(s) | 371, 403 |
| SduI | GDGCHC | 1 cut(s) | 404 |
| SfaNI | GCATC | 1 cut(s) | 356 |
| SfcI | CTRYAG | 1 cut(s) | 222 |
| SgeI | CNNG | 8 cut(s) | 83, 140, 161, 278, 314, 335, 343, 356 |
| Sse9I | AATT | 5 cut(s) | 103, 235, 334, 374, 475 |
| SsiI | CCGC | 1 cut(s) | 431 |
| SspMI | CTAG | 1 cut(s) | 128 |
| TaaI | ACNGT | 1 cut(s) | 67 |
| TaqII | GACCGA | 1 cut(s) | 344 |
| TasI | AATT | 5 cut(s) | 103, 235, 334, 374, 475 |
| TfiI | GAWTC | 3 cut(s) | 20, 26, 298 |
| Tru1I | TTAA | 1 cut(s) | 478 |
| Tru9I | TTAA | 1 cut(s) | 478 |
| TseFI | GTSAC | 1 cut(s) | 325 |
| TseI | GCWGC | 2 cut(s) | 425, 433 |
| Tsp45I | GTSAC | 1 cut(s) | 325 |
| TspDTI | ATGAA | 2 cut(s) | 69, 165 |
| XapI | RAATTY | 1 cut(s) | 334 |
| XspI | CTAG | 1 cut(s) | 128 |