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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 774 |
| AclWI | GGATC | 1 cut(s) | 784 |
| AcuI | CTGAAG | 1 cut(s) | 200 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 950 |
| AgsI | TTSAA | 6 cut(s) | 140, 430, 824, 1064, 1346, 1385 |
| AjiI | CACGTC | 1 cut(s) | 605 |
| AjnI | CCWGG | 3 cut(s) | 607, 949, 1226 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 248, 280, 444, 476 |
| AluBI | AGCT | 6 cut(s) | 235, 397, 842, 1106, 1310, 1405 |
| AluI | AGCT | 6 cut(s) | 235, 397, 842, 1106, 1310, 1405 |
| Alw26I | GTCTC | 4 cut(s) | 164, 200, 341, 1193 |
| AlwI | GGATC | 1 cut(s) | 784 |
| AoxI | GGCC | 2 cut(s) | 672, 1162 |
| ApeKI | GCWGC | 1 cut(s) | 842 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1371, 1403 |
| AspS9I | GGNCC | 1 cut(s) | 673 |
| AsuHPI | GGTGA | 8 cut(s) | 175, 332, 623, 807, 849, 1026, 1105, 1273 |
| AxyI | CCTNAGG | 1 cut(s) | 678 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1001, 1034 |
| BanII | GRGCYC | 1 cut(s) | 1151 |
| BauI | CACGAG | 1 cut(s) | 891 |
| BbvI | GCAGC | 1 cut(s) | 829 |
| BccI | CCATC | 2 cut(s) | 113, 337 |
| BceAI | ACGGC | 1 cut(s) | 783 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 882, 916 |
| BciT130I | CCWGG | 3 cut(s) | 609, 951, 1228 |
| BcoDI | GTCTC | 4 cut(s) | 164, 200, 341, 1193 |
| BfaI | CTAG | 2 cut(s) | 648, 756 |
| BfmI | CTRYAG | 1 cut(s) | 780 |
| BisI | GCNGC | 1 cut(s) | 843 |
| BlsI | GCNGC | 1 cut(s) | 844 |
| Bme1390I | CCNGG | 3 cut(s) | 609, 951, 1228 |
| BmgBI | CACGTC | 1 cut(s) | 605 |
| BmgT120I | GGNCC | 1 cut(s) | 673 |
| BmiI | GGNNCC | 1 cut(s) | 675 |
| BmrFI | CCNGG | 3 cut(s) | 609, 951, 1228 |
| BmsI | GCATC | 3 cut(s) | 49, 185, 886 |
| BoxI | GACNNNNGTC | 1 cut(s) | 811 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 512, 544 |
| BpmI | CTGGAG | 1 cut(s) | 933 |
| BpuEI | CTTGAG | 1 cut(s) | 419 |
| BsaJI | CCNNGG | 1 cut(s) | 765 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 476, 506, 619, 649, 955, 985 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 950 |
| Bse1I | ACTGG | 4 cut(s) | 150, 303, 501, 985 |
| Bse21I | CCTNAGG | 1 cut(s) | 678 |
| BseBI | CCWGG | 3 cut(s) | 609, 951, 1228 |
| BseDI | CCNNGG | 1 cut(s) | 765 |
| BseGI | GGATG | 1 cut(s) | 1357 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 950 |
| BseMII | CTCAG | 6 cut(s) | 69, 227, 570, 692, 1098, 1164 |
| BseNI | ACTGG | 4 cut(s) | 150, 303, 501, 985 |
| BseRI | GAGGAG | 1 cut(s) | 458 |
| BseXI | GCAGC | 1 cut(s) | 829 |
| BsgI | GTGCAG | 1 cut(s) | 828 |
| BshFI | GGCC | 2 cut(s) | 674, 1164 |
| BslFI | GGGAC | 1 cut(s) | 1247 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 950 |
| BsmAI | GTCTC | 4 cut(s) | 164, 200, 341, 1193 |
| BsmFI | GGGAC | 1 cut(s) | 1247 |
| BsmI | GAATGC | 1 cut(s) | 830 |
| BsnI | GGCC | 2 cut(s) | 674, 1164 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1151 |
| Bsp143I | GATC | 2 cut(s) | 776, 1023 |
| BspACI | CCGC | 1 cut(s) | 774 |
| BspANI | GGCC | 2 cut(s) | 674, 1164 |
| BspCNI | CTCAG | 6 cut(s) | 70, 228, 571, 691, 1099, 1163 |
| BspLI | GGNNCC | 1 cut(s) | 675 |
| BspMAI | CTGCAG | 1 cut(s) | 784 |
| BspPI | GGATC | 1 cut(s) | 784 |
| BspQI | GCTCTTC | 1 cut(s) | 225 |
| BsrI | ACTGG | 4 cut(s) | 150, 303, 501, 985 |
| BssECI | CCNNGG | 1 cut(s) | 765 |
| BssMI | GATC | 2 cut(s) | 776, 1023 |
| BssSI | CACGAG | 1 cut(s) | 891 |
| Bst2BI | CACGAG | 1 cut(s) | 891 |
| Bst2UI | CCWGG | 3 cut(s) | 609, 951, 1228 |
| Bst4CI | ACNGT | 3 cut(s) | 329, 698, 1204 |
| Bst6I | CTCTTC | 5 cut(s) | 225, 366, 408, 471, 1184 |
| BstC8I | GCNNGC | 2 cut(s) | 772, 901 |
| BstDEI | CTNAG | 6 cut(s) | 78, 236, 579, 678, 1107, 1150 |
| BstDSI | CCRYGG | 1 cut(s) | 765 |
| BstF5I | GGATG | 1 cut(s) | 1357 |
| BstKTI | GATC | 2 cut(s) | 779, 1026 |
| BstMAI | GTCTC | 4 cut(s) | 164, 200, 341, 1193 |
| BstMBI | GATC | 2 cut(s) | 776, 1023 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 839 |
| BstNI | CCWGG | 3 cut(s) | 609, 951, 1228 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 811 |
| BstSCI | CCNGG | 3 cut(s) | 607, 949, 1226 |
| BstSFI | CTRYAG | 1 cut(s) | 780 |
| BstV1I | GCAGC | 1 cut(s) | 829 |
| BstX2I | RGATCY | 1 cut(s) | 776 |
| BstYI | RGATCY | 1 cut(s) | 776 |
| Bsu36I | CCTNAGG | 1 cut(s) | 678 |
| BsuRI | GGCC | 2 cut(s) | 674, 1164 |
| BtgI | CCRYGG | 1 cut(s) | 765 |
| BtrI | CACGTC | 1 cut(s) | 605 |
| BtsCI | GGATG | 1 cut(s) | 1357 |
| BtsI | GCAGTG | 1 cut(s) | 5 |
| BtsIMutI | CAGTG | 3 cut(s) | 5, 157, 1283 |
| Cac8I | GCNNGC | 2 cut(s) | 772, 901 |
| Cfr13I | GGNCC | 1 cut(s) | 673 |
| CviAII | CATG | 1 cut(s) | 1331 |
| DdeI | CTNAG | 6 cut(s) | 78, 236, 579, 678, 1107, 1150 |
| DpnI | GATC | 2 cut(s) | 778, 1025 |
| DpnII | GATC | 2 cut(s) | 776, 1023 |
| Eam1104I | CTCTTC | 5 cut(s) | 225, 366, 408, 471, 1184 |
| EarI | CTCTTC | 5 cut(s) | 225, 366, 408, 471, 1184 |
| Eco24I | GRGCYC | 1 cut(s) | 1151 |
| Eco57I | CTGAAG | 1 cut(s) | 200 |
| Eco81I | CCTNAGG | 1 cut(s) | 678 |
| EcoO109I | RGGNCCY | 1 cut(s) | 673 |
| EcoRII | CCWGG | 3 cut(s) | 607, 949, 1226 |
| EcoT22I | ATGCAT | 1 cut(s) | 931 |
| EcoT38I | GRGCYC | 1 cut(s) | 1151 |
| FaeI | CATG | 1 cut(s) | 1334 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 516, 548 |
| FaqI | GGGAC | 1 cut(s) | 1247 |
| FatI | CATG | 1 cut(s) | 1330 |
| Fnu4HI | GCNGC | 1 cut(s) | 843 |
| FokI | GGATG | 1 cut(s) | 1364 |
| FriOI | GRGCYC | 1 cut(s) | 1151 |
| Fsp4HI | GCNGC | 1 cut(s) | 843 |
| FspBI | CTAG | 2 cut(s) | 648, 756 |
| GluI | GCNGC | 1 cut(s) | 843 |
| GsuI | CTGGAG | 1 cut(s) | 933 |
| HaeIII | GGCC | 2 cut(s) | 674, 1164 |
| Hin1II | CATG | 1 cut(s) | 1334 |
| HindIII | AAGCTT | 1 cut(s) | 395 |
| HinfI | GANTC | 7 cut(s) | 74, 80, 590, 623, 761, 854, 1154 |
| HphI | GGTGA | 8 cut(s) | 175, 332, 623, 807, 849, 1026, 1105, 1273 |
| Hpy166II | GTNNAC | 1 cut(s) | 1113 |
| Hpy188I | TCNGA | 6 cut(s) | 33, 79, 219, 724, 1023, 1153 |
| Hpy188III | TCNNGA | 1 cut(s) | 680 |
| Hpy8I | GTNNAC | 1 cut(s) | 1113 |
| Hpy99I | CGWCG | 1 cut(s) | 1361 |
| HpyAV | CCTTC | 2 cut(s) | 160, 475 |
| HpyCH4III | ACNGT | 3 cut(s) | 329, 698, 1204 |
| HpyCH4IV | ACGT | 2 cut(s) | 604, 1059 |
| HpyCH4V | TGCA | 7 cut(s) | 62, 176, 514, 782, 845, 903, 929 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 839 |
| HpyF3I | CTNAG | 6 cut(s) | 78, 236, 579, 678, 1107, 1150 |
| HpySE526I | ACGT | 2 cut(s) | 604, 1059 |
| Hsp92II | CATG | 1 cut(s) | 1334 |
| Kzo9I | GATC | 2 cut(s) | 776, 1023 |
| LguI | GCTCTTC | 1 cut(s) | 225 |
| Lsp1109I | GCAGC | 1 cut(s) | 829 |
| LweI | GCATC | 3 cut(s) | 49, 185, 886 |
| MaeI | CTAG | 2 cut(s) | 648, 756 |
| MaeII | ACGT | 2 cut(s) | 604, 1059 |
| MaeIII | GTNAC | 5 cut(s) | 3, 320, 329, 492, 813 |
| MalI | GATC | 2 cut(s) | 778, 1025 |
| MboI | GATC | 2 cut(s) | 776, 1023 |
| MfeI | CAATTG | 2 cut(s) | 551, 1072 |
| MflI | RGATCY | 1 cut(s) | 776 |
| MhlI | GDGCHC | 1 cut(s) | 1151 |
| MlyI | GAGTC | 4 cut(s) | 89, 599, 617, 755 |
| MmeI | TCCRAC | 1 cut(s) | 969 |
| Mph1103I | ATGCAT | 1 cut(s) | 931 |
| MseI | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| MslI | CAYNNNNRTG | 2 cut(s) | 566, 823 |
| MspA1I | CMGCKG | 1 cut(s) | 1310 |
| MspR9I | CCNGG | 3 cut(s) | 609, 951, 1228 |
| MunI | CAATTG | 2 cut(s) | 551, 1072 |
| Mva1269I | GAATGC | 1 cut(s) | 830 |
| MvaI | CCWGG | 3 cut(s) | 609, 951, 1228 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 839 |
| NdeII | GATC | 2 cut(s) | 776, 1023 |
| NlaIII | CATG | 1 cut(s) | 1334 |
| NlaIV | GGNNCC | 1 cut(s) | 675 |
| NmuCI | GTSAC | 3 cut(s) | 3, 320, 813 |
| NsiI | ATGCAT | 1 cut(s) | 931 |
| PciSI | GCTCTTC | 1 cut(s) | 225 |
| PctI | GAATGC | 1 cut(s) | 830 |
| PfeI | GAWTC | 3 cut(s) | 74, 854, 1154 |
| PkrI | GCNGC | 1 cut(s) | 844 |
| PleI | GAGTC | 4 cut(s) | 88, 598, 617, 755 |
| PpsI | GAGTC | 4 cut(s) | 88, 598, 617, 755 |
| PshAI | GACNNNNGTC | 1 cut(s) | 811 |
| Psp6I | CCWGG | 3 cut(s) | 607, 949, 1226 |
| PspGI | CCWGG | 3 cut(s) | 607, 949, 1226 |
| PspN4I | GGNNCC | 1 cut(s) | 675 |
| PspPI | GGNCC | 1 cut(s) | 673 |
| PstI | CTGCAG | 1 cut(s) | 784 |
| PsuI | RGATCY | 1 cut(s) | 776 |
| PvuII | CAGCTG | 1 cut(s) | 1310 |
| RseI | CAYNNNNRTG | 2 cut(s) | 566, 823 |
| SapI | GCTCTTC | 1 cut(s) | 225 |
| SaqAI | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| SatI | GCNGC | 1 cut(s) | 843 |
| Sau3AI | GATC | 2 cut(s) | 776, 1023 |
| Sau96I | GGNCC | 1 cut(s) | 673 |
| SchI | GAGTC | 4 cut(s) | 89, 599, 617, 755 |
| ScrFI | CCNGG | 3 cut(s) | 609, 951, 1228 |
| SduI | GDGCHC | 1 cut(s) | 1151 |
| SfaNI | GCATC | 3 cut(s) | 49, 185, 886 |
| SfcI | CTRYAG | 1 cut(s) | 780 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 566, 823 |
| SmlI | CTYRAG | 1 cut(s) | 398 |
| SmoI | CTYRAG | 1 cut(s) | 398 |
| SsiI | CCGC | 1 cut(s) | 774 |
| SspI | AATATT | 1 cut(s) | 1125 |
| SspMI | CTAG | 2 cut(s) | 648, 756 |
| StyD4I | CCNGG | 3 cut(s) | 607, 949, 1226 |
| TaaI | ACNGT | 3 cut(s) | 329, 698, 1204 |
| TaiI | ACGT | 2 cut(s) | 607, 1062 |
| TaqII | GACCGA | 1 cut(s) | 380 |
| TfiI | GAWTC | 3 cut(s) | 74, 854, 1154 |
| Tru1I | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| Tru9I | TTAA | 4 cut(s) | 51, 537, 876, 924 |
| TscAI | CASTG | 3 cut(s) | 12, 157, 1290 |
| TseFI | GTSAC | 3 cut(s) | 3, 320, 813 |
| TseI | GCWGC | 1 cut(s) | 842 |
| Tsp45I | GTSAC | 3 cut(s) | 3, 320, 813 |
| TspDTI | ATGAA | 3 cut(s) | 384, 396, 1202 |
| TspRI | CASTG | 3 cut(s) | 12, 157, 1290 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 157, 915 |
| XspI | CTAG | 2 cut(s) | 648, 756 |
| Zsp2I | ATGCAT | 1 cut(s) | 931 |