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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 350 |
| AcsI | RAATTY | 1 cut(s) | 118 |
| AfaI | GTAC | 1 cut(s) | 556 |
| AgsI | TTSAA | 4 cut(s) | 25, 41, 536, 548 |
| AleI | CACNNNNGTG | 1 cut(s) | 479 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 431, 463 |
| AluBI | AGCT | 3 cut(s) | 212, 225, 272 |
| AluI | AGCT | 3 cut(s) | 212, 225, 272 |
| Alw26I | GTCTC | 1 cut(s) | 668 |
| AlwI | GGATC | 1 cut(s) | 350 |
| ApeKI | GCWGC | 4 cut(s) | 209, 217, 284, 371 |
| ApoI | RAATTY | 1 cut(s) | 118 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 543 |
| AsuHPI | GGTGA | 3 cut(s) | 151, 617, 653 |
| BanII | GRGCYC | 1 cut(s) | 458 |
| BbsI | GAAGAC | 1 cut(s) | 587 |
| BbvI | GCAGC | 4 cut(s) | 221, 229, 271, 383 |
| BccI | CCATC | 1 cut(s) | 436 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 344, 378 |
| BcoDI | GTCTC | 1 cut(s) | 668 |
| BfmI | CTRYAG | 2 cut(s) | 363, 528 |
| BisI | GCNGC | 4 cut(s) | 210, 218, 285, 372 |
| BlpI | GCTNAGC | 1 cut(s) | 213 |
| BlsI | GCNGC | 4 cut(s) | 211, 219, 286, 373 |
| BmsI | GCATC | 2 cut(s) | 140, 383 |
| BoxI | GACNNNNGTC | 1 cut(s) | 507 |
| BpiI | GAAGAC | 1 cut(s) | 587 |
| BpmI | CTGGAG | 1 cut(s) | 107 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 213 |
| BsaI | GGTCTC | 1 cut(s) | 668 |
| BsaJI | CCNNGG | 1 cut(s) | 337 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 431, 461 |
| Bse1I | ACTGG | 1 cut(s) | 731 |
| BseDI | CCNNGG | 1 cut(s) | 337 |
| BseMII | CTCAG | 2 cut(s) | 105, 227 |
| BseNI | ACTGG | 1 cut(s) | 731 |
| BseXI | GCAGC | 4 cut(s) | 221, 229, 271, 383 |
| BsgI | GTGCAG | 2 cut(s) | 331, 379 |
| BslFI | GGGAC | 1 cut(s) | 319 |
| BsmAI | GTCTC | 1 cut(s) | 668 |
| BsmFI | GGGAC | 1 cut(s) | 319 |
| Bso31I | GGTCTC | 1 cut(s) | 668 |
| Bsp1286I | GDGCHC | 1 cut(s) | 458 |
| Bsp143I | GATC | 2 cut(s) | 307, 342 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 213 |
| Bsp19I | CCATGG | 1 cut(s) | 337 |
| BspCNI | CTCAG | 2 cut(s) | 106, 226 |
| BspMAI | CTGCAG | 1 cut(s) | 367 |
| BspPI | GGATC | 1 cut(s) | 350 |
| BspTNI | GGTCTC | 1 cut(s) | 668 |
| BsrI | ACTGG | 1 cut(s) | 731 |
| BssECI | CCNNGG | 1 cut(s) | 337 |
| BssMI | GATC | 2 cut(s) | 307, 342 |
| BssT1I | CCWWGG | 1 cut(s) | 337 |
| Bst4CI | ACNGT | 6 cut(s) | 7, 319, 352, 400, 447, 463 |
| BstDEI | CTNAG | 4 cut(s) | 56, 114, 213, 279 |
| BstDSI | CCRYGG | 1 cut(s) | 337 |
| BstKTI | GATC | 2 cut(s) | 310, 345 |
| BstMAI | GTCTC | 1 cut(s) | 668 |
| BstMBI | GATC | 2 cut(s) | 307, 342 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 371 |
| BstNSI | RCATGY | 1 cut(s) | 472 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 507 |
| BstSFI | CTRYAG | 2 cut(s) | 363, 528 |
| BstV1I | GCAGC | 4 cut(s) | 221, 229, 271, 383 |
| BstV2I | GAAGAC | 1 cut(s) | 587 |
| BstX2I | RGATCY | 1 cut(s) | 342 |
| BstYI | RGATCY | 1 cut(s) | 342 |
| BtgI | CCRYGG | 1 cut(s) | 337 |
| BtsI | GCAGTG | 1 cut(s) | 391 |
| BtsIMutI | CAGTG | 2 cut(s) | 391, 405 |
| Csp6I | GTAC | 1 cut(s) | 555 |
| CviAII | CATG | 4 cut(s) | 67, 76, 338, 469 |
| CviJI | RGCY | 6 cut(s) | 186, 212, 225, 272, 284, 456 |
| CviKI_1 | RGCY | 6 cut(s) | 186, 212, 225, 272, 284, 456 |
| CviQI | GTAC | 1 cut(s) | 555 |
| DdeI | CTNAG | 4 cut(s) | 56, 114, 213, 279 |
| DpnI | GATC | 2 cut(s) | 309, 344 |
| DpnII | GATC | 2 cut(s) | 307, 342 |
| Eco130I | CCWWGG | 1 cut(s) | 337 |
| Eco24I | GRGCYC | 1 cut(s) | 458 |
| Eco31I | GGTCTC | 1 cut(s) | 668 |
| Eco32I | GATATC | 1 cut(s) | 651 |
| EcoRV | GATATC | 1 cut(s) | 651 |
| EcoT14I | CCWWGG | 1 cut(s) | 337 |
| EcoT38I | GRGCYC | 1 cut(s) | 458 |
| ErhI | CCWWGG | 1 cut(s) | 337 |
| FaeI | CATG | 4 cut(s) | 70, 79, 341, 472 |
| FaiI | YATR | 7 cut(s) | 68, 77, 339, 470, 530, 566, 675 |
| FaqI | GGGAC | 1 cut(s) | 319 |
| FatI | CATG | 4 cut(s) | 66, 75, 337, 468 |
| Fnu4HI | GCNGC | 4 cut(s) | 210, 218, 285, 372 |
| FriOI | GRGCYC | 1 cut(s) | 458 |
| Fsp4HI | GCNGC | 4 cut(s) | 210, 218, 285, 372 |
| GluI | GCNGC | 4 cut(s) | 210, 218, 285, 372 |
| GsuI | CTGGAG | 1 cut(s) | 107 |
| Hin1II | CATG | 4 cut(s) | 70, 79, 341, 472 |
| HincII | GTYRAC | 2 cut(s) | 502, 612 |
| HindII | GTYRAC | 2 cut(s) | 502, 612 |
| HinfI | GANTC | 4 cut(s) | 193, 260, 359, 440 |
| HpaI | GTTAAC | 1 cut(s) | 612 |
| HphI | GGTGA | 3 cut(s) | 151, 617, 653 |
| Hpy166II | GTNNAC | 3 cut(s) | 466, 502, 612 |
| Hpy188I | TCNGA | 1 cut(s) | 513 |
| Hpy188III | TCNNGA | 2 cut(s) | 86, 451 |
| Hpy8I | GTNNAC | 3 cut(s) | 466, 502, 612 |
| HpyAV | CCTTC | 3 cut(s) | 136, 157, 320 |
| HpyCH4III | ACNGT | 6 cut(s) | 7, 319, 352, 400, 447, 463 |
| HpyCH4IV | ACGT | 1 cut(s) | 557 |
| HpyCH4V | TGCA | 5 cut(s) | 131, 348, 365, 396, 619 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 371 |
| HpyF3I | CTNAG | 4 cut(s) | 56, 114, 213, 279 |
| HpySE526I | ACGT | 1 cut(s) | 557 |
| Hsp92II | CATG | 4 cut(s) | 70, 79, 341, 472 |
| KspAI | GTTAAC | 1 cut(s) | 612 |
| Kzo9I | GATC | 2 cut(s) | 307, 342 |
| LmnI | GCTCC | 2 cut(s) | 230, 269 |
| LpnPI | CCDG | 5 cut(s) | 71, 464, 600, 624, 712 |
| Lsp1109I | GCAGC | 4 cut(s) | 221, 229, 271, 383 |
| LweI | GCATC | 2 cut(s) | 140, 383 |
| MaeII | ACGT | 1 cut(s) | 557 |
| MaeIII | GTNAC | 3 cut(s) | 109, 400, 441 |
| MalI | GATC | 2 cut(s) | 309, 344 |
| MboI | GATC | 2 cut(s) | 307, 342 |
| MboII | GAAGA | 8 cut(s) | 37, 260, 368, 419, 548, 589, 592, 711 |
| MfeI | CAATTG | 1 cut(s) | 311 |
| MflI | RGATCY | 1 cut(s) | 342 |
| MhlI | GDGCHC | 1 cut(s) | 458 |
| MluCI | AATT | 4 cut(s) | 19, 118, 167, 311 |
| MlyI | GAGTC | 2 cut(s) | 187, 449 |
| MnlI | CCTC | 8 cut(s) | 64, 82, 211, 243, 316, 399, 435, 632 |
| MroXI | GAANNNNTTC | 1 cut(s) | 543 |
| MseI | TTAA | 2 cut(s) | 611, 686 |
| MslI | CAYNNNNRTG | 2 cut(s) | 479, 639 |
| MunI | CAATTG | 1 cut(s) | 311 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 371 |
| NcoI | CCATGG | 1 cut(s) | 337 |
| NdeII | GATC | 2 cut(s) | 307, 342 |
| NlaIII | CATG | 4 cut(s) | 70, 79, 341, 472 |
| NmuCI | GTSAC | 3 cut(s) | 109, 400, 441 |
| NspI | RCATGY | 1 cut(s) | 472 |
| OliI | CACNNNNGTG | 1 cut(s) | 479 |
| PdmI | GAANNNNTTC | 1 cut(s) | 543 |
| PfeI | GAWTC | 2 cut(s) | 260, 359 |
| PkrI | GCNGC | 4 cut(s) | 211, 219, 286, 373 |
| PleI | GAGTC | 2 cut(s) | 187, 448 |
| PpsI | GAGTC | 2 cut(s) | 187, 448 |
| PshAI | GACNNNNGTC | 1 cut(s) | 507 |
| PstI | CTGCAG | 1 cut(s) | 367 |
| PsuI | RGATCY | 1 cut(s) | 342 |
| RsaI | GTAC | 1 cut(s) | 556 |
| RsaNI | GTAC | 1 cut(s) | 555 |
| RseI | CAYNNNNRTG | 2 cut(s) | 479, 639 |
| SaqAI | TTAA | 2 cut(s) | 611, 686 |
| SatI | GCNGC | 4 cut(s) | 210, 218, 285, 372 |
| Sau3AI | GATC | 2 cut(s) | 307, 342 |
| SchI | GAGTC | 2 cut(s) | 187, 449 |
| SduI | GDGCHC | 1 cut(s) | 458 |
| SfaNI | GCATC | 2 cut(s) | 140, 383 |
| SfcI | CTRYAG | 2 cut(s) | 363, 528 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 479, 639 |
| Sse9I | AATT | 4 cut(s) | 19, 118, 167, 311 |
| StyI | CCWWGG | 1 cut(s) | 337 |
| TaaI | ACNGT | 6 cut(s) | 7, 319, 352, 400, 447, 463 |
| TaiI | ACGT | 1 cut(s) | 560 |
| TaqI | TCGA | 2 cut(s) | 161, 354 |
| TasI | AATT | 4 cut(s) | 19, 118, 167, 311 |
| TfiI | GAWTC | 2 cut(s) | 260, 359 |
| Tru1I | TTAA | 2 cut(s) | 611, 686 |
| Tru9I | TTAA | 2 cut(s) | 611, 686 |
| TscAI | CASTG | 2 cut(s) | 398, 405 |
| TseFI | GTSAC | 3 cut(s) | 109, 400, 441 |
| TseI | GCWGC | 4 cut(s) | 209, 217, 284, 371 |
| Tsp45I | GTSAC | 3 cut(s) | 109, 400, 441 |
| TspDTI | ATGAA | 1 cut(s) | 273 |
| TspRI | CASTG | 2 cut(s) | 398, 405 |
| XapI | RAATTY | 1 cut(s) | 118 |
| XceI | RCATGY | 1 cut(s) | 472 |
| XmnI | GAANNNNTTC | 1 cut(s) | 543 |