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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 421 |
| AcoI | YGGCCR | 1 cut(s) | 251 |
| AcsI | RAATTY | 1 cut(s) | 390 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 620 |
| AgsI | TTSAA | 3 cut(s) | 88, 172, 494 |
| AjiI | CACGTC | 1 cut(s) | 263 |
| AjnI | CCWGG | 1 cut(s) | 265 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 102, 134 |
| AluBI | AGCT | 4 cut(s) | 55, 76, 512, 688 |
| AluI | AGCT | 4 cut(s) | 55, 76, 512, 688 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 699 |
| Ama87I | CYCGRG | 1 cut(s) | 56 |
| AoxI | GGCC | 2 cut(s) | 251, 330 |
| ApeKI | GCWGC | 1 cut(s) | 512 |
| ApoI | RAATTY | 1 cut(s) | 390 |
| AspLEI | GCGC | 1 cut(s) | 434 |
| AsuC2I | CCSGG | 1 cut(s) | 621 |
| AsuHPI | GGTGA | 3 cut(s) | 281, 477, 519 |
| AvaI | CYCGRG | 1 cut(s) | 56 |
| AxyI | CCTNAGG | 1 cut(s) | 540 |
| BaeGI | GKGCMC | 1 cut(s) | 626 |
| BalI | TGGCCA | 1 cut(s) | 253 |
| BbsI | GAAGAC | 1 cut(s) | 26 |
| BbvI | GCAGC | 1 cut(s) | 499 |
| BciT130I | CCWGG | 1 cut(s) | 267 |
| BcnI | CCSGG | 1 cut(s) | 621 |
| BfaI | CTAG | 1 cut(s) | 306 |
| BfuAI | ACCTGC | 1 cut(s) | 421 |
| BisI | GCNGC | 1 cut(s) | 513 |
| BlsI | GCNGC | 1 cut(s) | 514 |
| Bme1390I | CCNGG | 2 cut(s) | 267, 621 |
| BmeT110I | CYCGRG | 1 cut(s) | 56 |
| BmgBI | CACGTC | 1 cut(s) | 263 |
| BmiI | GGNNCC | 1 cut(s) | 456 |
| BmrFI | CCNGG | 2 cut(s) | 267, 621 |
| BmsI | GCATC | 2 cut(s) | 443, 556 |
| BoxI | GACNNNNGTC | 1 cut(s) | 481 |
| BpiI | GAAGAC | 1 cut(s) | 26 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 170, 202 |
| BpuEI | CTTGAG | 1 cut(s) | 366 |
| BpuMI | CCSGG | 1 cut(s) | 621 |
| BsaJI | CCNNGG | 1 cut(s) | 472 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 134, 164, 277, 307, 347, 377, 625, 655 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 620 |
| Bse21I | CCTNAGG | 1 cut(s) | 540 |
| BseBI | CCWGG | 1 cut(s) | 267 |
| BseDI | CCNNGG | 1 cut(s) | 472 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 620 |
| BseMII | CTCAG | 4 cut(s) | 228, 350, 429, 531 |
| BseRI | GAGGAG | 3 cut(s) | 116, 279, 282 |
| BseSI | GKGCMC | 1 cut(s) | 626 |
| BseXI | GCAGC | 1 cut(s) | 499 |
| BsgI | GTGCAG | 1 cut(s) | 498 |
| Bsh1285I | CGRYCG | 1 cut(s) | 472 |
| BshFI | GGCC | 2 cut(s) | 253, 332 |
| BsiEI | CGRYCG | 1 cut(s) | 472 |
| BsiHKCI | CYCGRG | 1 cut(s) | 56 |
| BsiSI | CCGG | 1 cut(s) | 620 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 620 |
| BsmI | GAATGC | 1 cut(s) | 500 |
| BsnI | GGCC | 2 cut(s) | 253, 332 |
| BsoBI | CYCGRG | 1 cut(s) | 56 |
| Bsp1286I | GDGCHC | 1 cut(s) | 626 |
| BspANI | GGCC | 2 cut(s) | 253, 332 |
| BspCNI | CTCAG | 4 cut(s) | 229, 349, 430, 532 |
| BspLI | GGNNCC | 1 cut(s) | 456 |
| BspMI | ACCTGC | 1 cut(s) | 421 |
| BspQI | GCTCTTC | 1 cut(s) | 66 |
| BssECI | CCNNGG | 1 cut(s) | 472 |
| Bst2UI | CCWGG | 1 cut(s) | 267 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| BstC8I | GCNNGC | 3 cut(s) | 430, 571, 672 |
| BstDEI | CTNAG | 5 cut(s) | 77, 237, 336, 438, 540 |
| BstHHI | GCGC | 1 cut(s) | 434 |
| BstMCI | CGRYCG | 1 cut(s) | 472 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 509, 676, 685 |
| BstNI | CCWGG | 1 cut(s) | 267 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 481 |
| BstSCI | CCNGG | 2 cut(s) | 265, 619 |
| BstSLI | GKGCMC | 1 cut(s) | 626 |
| BstV1I | GCAGC | 1 cut(s) | 499 |
| BstV2I | GAAGAC | 1 cut(s) | 26 |
| Bsu36I | CCTNAGG | 1 cut(s) | 540 |
| BsuRI | GGCC | 2 cut(s) | 253, 332 |
| BtrI | CACGTC | 1 cut(s) | 263 |
| BtsIMutI | CAGTG | 1 cut(s) | 188 |
| BveI | ACCTGC | 1 cut(s) | 421 |
| Cac8I | GCNNGC | 3 cut(s) | 430, 571, 672 |
| CaiI | CAGNNNCTG | 1 cut(s) | 699 |
| CfoI | GCGC | 1 cut(s) | 434 |
| CviJI | RGCY | 9 cut(s) | 55, 76, 185, 253, 332, 346, 512, 679, 688 |
| CviKI_1 | RGCY | 9 cut(s) | 55, 76, 185, 253, 332, 346, 512, 679, 688 |
| DdeI | CTNAG | 5 cut(s) | 77, 237, 336, 438, 540 |
| EaeI | YGGCCR | 1 cut(s) | 251 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| EarI | CTCTTC | 3 cut(s) | 24, 66, 129 |
| Eco81I | CCTNAGG | 1 cut(s) | 540 |
| Eco88I | CYCGRG | 1 cut(s) | 56 |
| EcoRI | GAATTC | 1 cut(s) | 390 |
| EcoRII | CCWGG | 1 cut(s) | 265 |
| EcoT22I | ATGCAT | 1 cut(s) | 601 |
| FaiI | YATR | 4 cut(s) | 149, 221, 246, 668 |
| Fnu4HI | GCNGC | 1 cut(s) | 513 |
| Fsp4HI | GCNGC | 1 cut(s) | 513 |
| FspBI | CTAG | 1 cut(s) | 306 |
| GlaI | GCGC | 1 cut(s) | 433 |
| GluI | GCNGC | 1 cut(s) | 513 |
| HaeIII | GGCC | 2 cut(s) | 253, 332 |
| HapII | CCGG | 1 cut(s) | 620 |
| HhaI | GCGC | 1 cut(s) | 434 |
| Hin6I | GCGC | 1 cut(s) | 432 |
| HinP1I | GCGC | 1 cut(s) | 432 |
| HindIII | AAGCTT | 1 cut(s) | 686 |
| HinfI | GANTC | 1 cut(s) | 281 |
| HpaII | CCGG | 1 cut(s) | 620 |
| HphI | GGTGA | 3 cut(s) | 281, 477, 519 |
| Hpy188I | TCNGA | 1 cut(s) | 439 |
| Hpy188III | TCNNGA | 2 cut(s) | 338, 539 |
| HpyAV | CCTTC | 1 cut(s) | 133 |
| HpyCH4IV | ACGT | 2 cut(s) | 262, 626 |
| HpyCH4V | TGCA | 4 cut(s) | 515, 573, 599, 670 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 509, 676, 685 |
| HpyF3I | CTNAG | 5 cut(s) | 77, 237, 336, 438, 540 |
| HpySE526I | ACGT | 2 cut(s) | 262, 626 |
| HspAI | GCGC | 1 cut(s) | 432 |
| LguI | GCTCTTC | 1 cut(s) | 66 |
| LpnPI | CCDG | 7 cut(s) | 252, 279, 323, 380, 426, 552, 633 |
| Lsp1109I | GCAGC | 1 cut(s) | 499 |
| LweI | GCATC | 2 cut(s) | 443, 556 |
| MaeI | CTAG | 1 cut(s) | 306 |
| MaeII | ACGT | 2 cut(s) | 262, 626 |
| MaeIII | GTNAC | 2 cut(s) | 483, 627 |
| MboII | GAAGA | 8 cut(s) | 31, 41, 46, 80, 83, 146, 575, 674 |
| MfeI | CAATTG | 1 cut(s) | 209 |
| MhlI | GDGCHC | 1 cut(s) | 626 |
| MlsI | TGGCCA | 1 cut(s) | 253 |
| MluCI | AATT | 6 cut(s) | 41, 117, 174, 209, 390, 548 |
| MluNI | TGGCCA | 1 cut(s) | 253 |
| MlyI | GAGTC | 1 cut(s) | 275 |
| Mox20I | TGGCCA | 1 cut(s) | 253 |
| Mph1103I | ATGCAT | 1 cut(s) | 601 |
| MscI | TGGCCA | 1 cut(s) | 253 |
| MseI | TTAA | 3 cut(s) | 195, 546, 594 |
| MslI | CAYNNNNRTG | 2 cut(s) | 224, 493 |
| Msp20I | TGGCCA | 1 cut(s) | 253 |
| MspI | CCGG | 1 cut(s) | 620 |
| MspR9I | CCNGG | 2 cut(s) | 267, 621 |
| MunI | CAATTG | 1 cut(s) | 209 |
| Mva1269I | GAATGC | 1 cut(s) | 500 |
| MvaI | CCWGG | 1 cut(s) | 267 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 509, 676, 685 |
| NciI | CCSGG | 1 cut(s) | 621 |
| NlaIV | GGNNCC | 1 cut(s) | 456 |
| NmuCI | GTSAC | 1 cut(s) | 483 |
| NsiI | ATGCAT | 1 cut(s) | 601 |
| PaeR7I | CTCGAG | 1 cut(s) | 56 |
| PciSI | GCTCTTC | 1 cut(s) | 66 |
| PctI | GAATGC | 1 cut(s) | 500 |
| PkrI | GCNGC | 1 cut(s) | 514 |
| PleI | GAGTC | 1 cut(s) | 275 |
| PpsI | GAGTC | 1 cut(s) | 275 |
| PshAI | GACNNNNGTC | 1 cut(s) | 481 |
| Psp6I | CCWGG | 1 cut(s) | 265 |
| PspGI | CCWGG | 1 cut(s) | 265 |
| PspN4I | GGNNCC | 1 cut(s) | 456 |
| PspXI | VCTCGAGB | 1 cut(s) | 56 |
| PstNI | CAGNNNCTG | 1 cut(s) | 699 |
| RseI | CAYNNNNRTG | 2 cut(s) | 224, 493 |
| SapI | GCTCTTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 3 cut(s) | 195, 546, 594 |
| SatI | GCNGC | 1 cut(s) | 513 |
| SchI | GAGTC | 1 cut(s) | 275 |
| ScrFI | CCNGG | 2 cut(s) | 267, 621 |
| SduI | GDGCHC | 1 cut(s) | 626 |
| SfaNI | GCATC | 2 cut(s) | 443, 556 |
| Sfr274I | CTCGAG | 1 cut(s) | 56 |
| SlaI | CTCGAG | 1 cut(s) | 56 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 224, 493 |
| SmlI | CTYRAG | 2 cut(s) | 56, 381 |
| SmoI | CTYRAG | 2 cut(s) | 56, 381 |
| Sse9I | AATT | 6 cut(s) | 41, 117, 174, 209, 390, 548 |
| SspMI | CTAG | 1 cut(s) | 306 |
| StyD4I | CCNGG | 2 cut(s) | 265, 619 |
| TaiI | ACGT | 2 cut(s) | 265, 629 |
| TaqI | TCGA | 1 cut(s) | 57 |
| TaqII | GACCGA | 1 cut(s) | 486 |
| TasI | AATT | 6 cut(s) | 41, 117, 174, 209, 390, 548 |
| Tru1I | TTAA | 3 cut(s) | 195, 546, 594 |
| Tru9I | TTAA | 3 cut(s) | 195, 546, 594 |
| TscAI | CASTG | 1 cut(s) | 195 |
| TseFI | GTSAC | 1 cut(s) | 483 |
| TseI | GCWGC | 1 cut(s) | 512 |
| Tsp45I | GTSAC | 1 cut(s) | 483 |
| TspDTI | ATGAA | 3 cut(s) | 42, 54, 512 |
| TspGWI | ACGGA | 3 cut(s) | 467, 575, 644 |
| TspRI | CASTG | 1 cut(s) | 195 |
| XapI | RAATTY | 1 cut(s) | 390 |
| XhoI | CTCGAG | 1 cut(s) | 56 |
| XspI | CTAG | 1 cut(s) | 306 |
| Zsp2I | ATGCAT | 1 cut(s) | 601 |