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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1150 |
| AccI | GTMKAC | 2 cut(s) | 1155, 1176 |
| AccIII | TCCGGA | 1 cut(s) | 79 |
| AclWI | GGATC | 1 cut(s) | 509 |
| AcoI | YGGCCR | 4 cut(s) | 1298, 1315, 1375, 1392 |
| AcsI | RAATTY | 1 cut(s) | 343 |
| AcuI | CTGAAG | 2 cut(s) | 153, 182 |
| AgsI | TTSAA | 6 cut(s) | 250, 428, 698, 710, 938, 949 |
| AjnI | CCWGG | 1 cut(s) | 1191 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 593, 625 |
| AluBI | AGCT | 7 cut(s) | 101, 196, 259, 385, 431, 662, 1138 |
| AluI | AGCT | 7 cut(s) | 101, 196, 259, 385, 431, 662, 1138 |
| Alw26I | GTCTC | 1 cut(s) | 830 |
| AlwI | GGATC | 1 cut(s) | 509 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1004 |
| Aor13HI | TCCGGA | 1 cut(s) | 79 |
| AoxI | GGCC | 5 cut(s) | 987, 1298, 1315, 1375, 1392 |
| ApeKI | GCWGC | 6 cut(s) | 377, 431, 443, 530, 533, 1094 |
| ApoI | RAATTY | 1 cut(s) | 343 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1335, 1367 |
| AseI | ATTAAT | 1 cut(s) | 422 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 705 |
| AspS9I | GGNCC | 1 cut(s) | 1189 |
| AsuHPI | GGTGA | 3 cut(s) | 316, 376, 815 |
| AvaII | GGWCC | 1 cut(s) | 1189 |
| BalI | TGGCCA | 4 cut(s) | 1300, 1317, 1377, 1394 |
| BanII | GRGCYC | 1 cut(s) | 620 |
| BbsI | GAAGAC | 2 cut(s) | 749, 1391 |
| BbvCI | CCTCAGC | 1 cut(s) | 1146 |
| BbvI | GCAGC | 6 cut(s) | 389, 418, 430, 542, 545, 1081 |
| BccI | CCATC | 4 cut(s) | 598, 1021, 1081, 1243 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 503, 537 |
| BciT130I | CCWGG | 1 cut(s) | 1193 |
| BclI | TGATCA | 1 cut(s) | 106 |
| BcoDI | GTCTC | 1 cut(s) | 830 |
| BfaI | CTAG | 1 cut(s) | 1019 |
| BfmI | CTRYAG | 4 cut(s) | 231, 522, 690, 1095 |
| BfuAI | ACCTGC | 1 cut(s) | 1150 |
| BisI | GCNGC | 6 cut(s) | 378, 432, 444, 531, 534, 1095 |
| BlsI | GCNGC | 6 cut(s) | 379, 433, 445, 532, 535, 1096 |
| Bme1390I | CCNGG | 1 cut(s) | 1193 |
| Bme18I | GGWCC | 1 cut(s) | 1189 |
| BmgT120I | GGNCC | 1 cut(s) | 1189 |
| BmrFI | CCNGG | 1 cut(s) | 1193 |
| BmrI | ACTGGG | 2 cut(s) | 22, 1168 |
| BmsI | GCATC | 5 cut(s) | 365, 443, 545, 943, 991 |
| BmuI | ACTGGG | 2 cut(s) | 22, 1168 |
| BoxI | GACNNNNGTC | 1 cut(s) | 669 |
| BpiI | GAAGAC | 2 cut(s) | 749, 1391 |
| BpmI | CTGGAG | 1 cut(s) | 332 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1146 |
| BsaI | GGTCTC | 1 cut(s) | 830 |
| BsaJI | CCNNGG | 1 cut(s) | 496 |
| BsaWI | WCCGGW | 3 cut(s) | 79, 800, 1057 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 593, 623 |
| Bse1I | ACTGG | 8 cut(s) | 17, 893, 1163, 1226, 1301, 1318, 1378, 1395 |
| BseAI | TCCGGA | 1 cut(s) | 79 |
| BseBI | CCWGG | 1 cut(s) | 1193 |
| BseDI | CCNNGG | 1 cut(s) | 496 |
| BseGI | GGATG | 1 cut(s) | 1069 |
| BseMII | CTCAG | 2 cut(s) | 387, 1137 |
| BseNI | ACTGG | 8 cut(s) | 17, 893, 1163, 1226, 1301, 1318, 1378, 1395 |
| BseRI | GAGGAG | 5 cut(s) | 62, 224, 227, 1178, 1219 |
| BseXI | GCAGC | 6 cut(s) | 389, 418, 430, 542, 545, 1081 |
| BsgI | GTGCAG | 2 cut(s) | 490, 541 |
| BshFI | GGCC | 5 cut(s) | 989, 1300, 1317, 1377, 1394 |
| BsiSI | CCGG | 3 cut(s) | 80, 801, 1058 |
| BslFI | GGGAC | 1 cut(s) | 478 |
| BsmAI | GTCTC | 1 cut(s) | 830 |
| BsmFI | GGGAC | 1 cut(s) | 478 |
| BsnI | GGCC | 5 cut(s) | 989, 1300, 1317, 1377, 1394 |
| Bso31I | GGTCTC | 1 cut(s) | 830 |
| Bsp1286I | GDGCHC | 1 cut(s) | 620 |
| Bsp13I | TCCGGA | 1 cut(s) | 79 |
| Bsp143I | GATC | 4 cut(s) | 56, 106, 466, 501 |
| Bsp19I | CCATGG | 1 cut(s) | 496 |
| BspANI | GGCC | 5 cut(s) | 989, 1300, 1317, 1377, 1394 |
| BspCNI | CTCAG | 2 cut(s) | 386, 1138 |
| BspEI | TCCGGA | 1 cut(s) | 79 |
| BspMAI | CTGCAG | 3 cut(s) | 235, 526, 1099 |
| BspMI | ACCTGC | 1 cut(s) | 1150 |
| BspPI | GGATC | 1 cut(s) | 509 |
| BspTNI | GGTCTC | 1 cut(s) | 830 |
| BsrI | ACTGG | 8 cut(s) | 17, 893, 1163, 1226, 1301, 1318, 1378, 1395 |
| BssECI | CCNNGG | 1 cut(s) | 496 |
| BssMI | GATC | 4 cut(s) | 56, 106, 466, 501 |
| BssNAI | GTATAC | 1 cut(s) | 1177 |
| BssT1I | CCWWGG | 1 cut(s) | 496 |
| Bst1107I | GTATAC | 1 cut(s) | 1177 |
| Bst2UI | CCWGG | 1 cut(s) | 1193 |
| Bst6I | CTCTTC | 3 cut(s) | 39, 267, 933 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1103 |
| BstDEI | CTNAG | 5 cut(s) | 136, 281, 373, 438, 1146 |
| BstDSI | CCRYGG | 1 cut(s) | 496 |
| BstF5I | GGATG | 1 cut(s) | 1069 |
| BstKTI | GATC | 4 cut(s) | 59, 109, 469, 504 |
| BstMAI | GTCTC | 1 cut(s) | 830 |
| BstMBI | GATC | 4 cut(s) | 56, 106, 466, 501 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 440, 530, 1103 |
| BstNI | CCWGG | 1 cut(s) | 1193 |
| BstNSI | RCATGY | 3 cut(s) | 634, 1257, 1281 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 669 |
| BstSCI | CCNGG | 1 cut(s) | 1191 |
| BstSFI | CTRYAG | 4 cut(s) | 231, 522, 690, 1095 |
| BstV1I | GCAGC | 6 cut(s) | 389, 418, 430, 542, 545, 1081 |
| BstV2I | GAAGAC | 2 cut(s) | 749, 1391 |
| BstX2I | RGATCY | 1 cut(s) | 501 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1337 |
| BstYI | RGATCY | 1 cut(s) | 501 |
| BstZ17I | GTATAC | 1 cut(s) | 1177 |
| BsuRI | GGCC | 5 cut(s) | 989, 1300, 1317, 1377, 1394 |
| BtgI | CCRYGG | 1 cut(s) | 496 |
| BtsCI | GGATG | 1 cut(s) | 1069 |
| BtsI | GCAGTG | 2 cut(s) | 553, 992 |
| BveI | ACCTGC | 1 cut(s) | 1150 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1004 |
| Cfr13I | GGNCC | 1 cut(s) | 1189 |
| CseI | GACGC | 1 cut(s) | 1100 |
| DdeI | CTNAG | 5 cut(s) | 136, 281, 373, 438, 1146 |
| DpnI | GATC | 4 cut(s) | 58, 108, 468, 503 |
| DpnII | GATC | 4 cut(s) | 56, 106, 466, 501 |
| EaeI | YGGCCR | 4 cut(s) | 1298, 1315, 1375, 1392 |
| Eam1104I | CTCTTC | 3 cut(s) | 39, 267, 933 |
| EarI | CTCTTC | 3 cut(s) | 39, 267, 933 |
| Eco130I | CCWWGG | 1 cut(s) | 496 |
| Eco147I | AGGCCT | 1 cut(s) | 989 |
| Eco24I | GRGCYC | 1 cut(s) | 620 |
| Eco31I | GGTCTC | 1 cut(s) | 830 |
| Eco47I | GGWCC | 1 cut(s) | 1189 |
| Eco57I | CTGAAG | 2 cut(s) | 153, 182 |
| EcoRII | CCWGG | 1 cut(s) | 1191 |
| EcoT14I | CCWWGG | 1 cut(s) | 496 |
| EcoT38I | GRGCYC | 1 cut(s) | 620 |
| ErhI | CCWWGG | 1 cut(s) | 496 |
| FaqI | GGGAC | 1 cut(s) | 478 |
| FbaI | TGATCA | 1 cut(s) | 106 |
| FblI | GTMKAC | 2 cut(s) | 1155, 1176 |
| Fnu4HI | GCNGC | 6 cut(s) | 378, 432, 444, 531, 534, 1095 |
| FokI | GGATG | 1 cut(s) | 1076 |
| FriOI | GRGCYC | 1 cut(s) | 620 |
| Fsp4HI | GCNGC | 6 cut(s) | 378, 432, 444, 531, 534, 1095 |
| FspBI | CTAG | 1 cut(s) | 1019 |
| GluI | GCNGC | 6 cut(s) | 378, 432, 444, 531, 534, 1095 |
| GsuI | CTGGAG | 1 cut(s) | 332 |
| HaeIII | GGCC | 5 cut(s) | 989, 1300, 1317, 1377, 1394 |
| HapII | CCGG | 3 cut(s) | 80, 801, 1058 |
| HgaI | GACGC | 1 cut(s) | 1100 |
| HincII | GTYRAC | 1 cut(s) | 774 |
| HindII | GTYRAC | 1 cut(s) | 774 |
| HinfI | GANTC | 8 cut(s) | 29, 35, 65, 307, 371, 518, 602, 1209 |
| HpaI | GTTAAC | 1 cut(s) | 774 |
| HpaII | CCGG | 3 cut(s) | 80, 801, 1058 |
| HphI | GGTGA | 3 cut(s) | 316, 376, 815 |
| Hpy166II | GTNNAC | 4 cut(s) | 628, 774, 1156, 1177 |
| Hpy188I | TCNGA | 7 cut(s) | 34, 40, 61, 111, 172, 201, 675 |
| Hpy188III | TCNNGA | 7 cut(s) | 80, 311, 613, 1070, 1213, 1239, 1364 |
| Hpy8I | GTNNAC | 4 cut(s) | 628, 774, 1156, 1177 |
| Hpy99I | CGWCG | 1 cut(s) | 364 |
| HpyAV | CCTTC | 5 cut(s) | 77, 361, 401, 479, 979 |
| HpyCH4IV | ACGT | 1 cut(s) | 1009 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 440, 530, 1103 |
| HpyF3I | CTNAG | 5 cut(s) | 136, 281, 373, 438, 1146 |
| HpySE526I | ACGT | 1 cut(s) | 1009 |
| Kpn2I | TCCGGA | 1 cut(s) | 79 |
| Ksp22I | TGATCA | 1 cut(s) | 106 |
| KspAI | GTTAAC | 1 cut(s) | 774 |
| Kzo9I | GATC | 4 cut(s) | 56, 106, 466, 501 |
| LmnI | GCTCC | 2 cut(s) | 98, 390 |
| Lsp1109I | GCAGC | 6 cut(s) | 389, 418, 430, 542, 545, 1081 |
| LweI | GCATC | 5 cut(s) | 365, 443, 545, 943, 991 |
| MaeI | CTAG | 1 cut(s) | 1019 |
| MaeII | ACGT | 1 cut(s) | 1009 |
| MalI | GATC | 4 cut(s) | 58, 108, 468, 503 |
| MboI | GATC | 4 cut(s) | 56, 106, 466, 501 |
| MflI | RGATCY | 1 cut(s) | 501 |
| MhlI | GDGCHC | 1 cut(s) | 620 |
| MlsI | TGGCCA | 4 cut(s) | 1300, 1317, 1377, 1394 |
| MluCI | AATT | 8 cut(s) | 112, 141, 244, 343, 419, 423, 1043, 1180 |
| MluNI | TGGCCA | 4 cut(s) | 1300, 1317, 1377, 1394 |
| MlyI | GAGTC | 3 cut(s) | 365, 611, 1218 |
| Mox20I | TGGCCA | 4 cut(s) | 1300, 1317, 1377, 1394 |
| MroI | TCCGGA | 1 cut(s) | 79 |
| MroXI | GAANNNNTTC | 1 cut(s) | 705 |
| MscI | TGGCCA | 4 cut(s) | 1300, 1317, 1377, 1394 |
| MseI | TTAA | 3 cut(s) | 422, 773, 848 |
| MslI | CAYNNNNRTG | 4 cut(s) | 801, 1258, 1282, 1335 |
| Msp20I | TGGCCA | 4 cut(s) | 1300, 1317, 1377, 1394 |
| MspI | CCGG | 3 cut(s) | 80, 801, 1058 |
| MspR9I | CCNGG | 1 cut(s) | 1193 |
| MvaI | CCWGG | 1 cut(s) | 1193 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 440, 530, 1103 |
| NcoI | CCATGG | 1 cut(s) | 496 |
| NdeII | GATC | 4 cut(s) | 56, 106, 466, 501 |
| NspI | RCATGY | 3 cut(s) | 634, 1257, 1281 |
| PceI | AGGCCT | 1 cut(s) | 989 |
| PdmI | GAANNNNTTC | 1 cut(s) | 705 |
| PfeI | GAWTC | 5 cut(s) | 29, 35, 65, 307, 518 |
| PkrI | GCNGC | 6 cut(s) | 379, 433, 445, 532, 535, 1096 |
| PleI | GAGTC | 3 cut(s) | 365, 610, 1217 |
| PpsI | GAGTC | 3 cut(s) | 365, 610, 1217 |
| PshAI | GACNNNNGTC | 1 cut(s) | 669 |
| PshBI | ATTAAT | 1 cut(s) | 422 |
| Psp6I | CCWGG | 1 cut(s) | 1191 |
| PspGI | CCWGG | 1 cut(s) | 1191 |
| PspPI | GGNCC | 1 cut(s) | 1189 |
| PstI | CTGCAG | 3 cut(s) | 235, 526, 1099 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1004 |
| PsuI | RGATCY | 1 cut(s) | 501 |
| RseI | CAYNNNNRTG | 4 cut(s) | 801, 1258, 1282, 1335 |
| SaqAI | TTAA | 3 cut(s) | 422, 773, 848 |
| SatI | GCNGC | 6 cut(s) | 378, 432, 444, 531, 534, 1095 |
| Sau3AI | GATC | 4 cut(s) | 56, 106, 466, 501 |
| Sau96I | GGNCC | 1 cut(s) | 1189 |
| SchI | GAGTC | 3 cut(s) | 365, 611, 1218 |
| ScrFI | CCNGG | 1 cut(s) | 1193 |
| SduI | GDGCHC | 1 cut(s) | 620 |
| SfaNI | GCATC | 5 cut(s) | 365, 443, 545, 943, 991 |
| SfcI | CTRYAG | 4 cut(s) | 231, 522, 690, 1095 |
| SinI | GGWCC | 1 cut(s) | 1189 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 801, 1258, 1282, 1335 |
| Sse9I | AATT | 8 cut(s) | 112, 141, 244, 343, 419, 423, 1043, 1180 |
| SseBI | AGGCCT | 1 cut(s) | 989 |
| SspMI | CTAG | 1 cut(s) | 1019 |
| StuI | AGGCCT | 1 cut(s) | 989 |
| StyD4I | CCNGG | 1 cut(s) | 1191 |
| StyI | CCWWGG | 1 cut(s) | 496 |
| TaiI | ACGT | 1 cut(s) | 1012 |
| TaqI | TCGA | 3 cut(s) | 473, 513, 581 |
| TaqII | GACCGA | 1 cut(s) | 353 |
| TasI | AATT | 8 cut(s) | 112, 141, 244, 343, 419, 423, 1043, 1180 |
| TfiI | GAWTC | 5 cut(s) | 29, 35, 65, 307, 518 |
| Tru1I | TTAA | 3 cut(s) | 422, 773, 848 |
| Tru9I | TTAA | 3 cut(s) | 422, 773, 848 |
| TseI | GCWGC | 6 cut(s) | 377, 431, 443, 530, 533, 1094 |
| TspDTI | ATGAA | 4 cut(s) | 78, 279, 432, 998 |
| VpaK11BI | GGWCC | 1 cut(s) | 1189 |
| VspI | ATTAAT | 1 cut(s) | 422 |
| XapI | RAATTY | 1 cut(s) | 343 |
| XceI | RCATGY | 3 cut(s) | 634, 1257, 1281 |
| XmiI | GTMKAC | 2 cut(s) | 1155, 1176 |
| XmnI | GAANNNNTTC | 1 cut(s) | 705 |
| XspI | CTAG | 1 cut(s) | 1019 |