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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 88, 197 |
| AjiI | CACGTC | 1 cut(s) | 263 |
| AjnI | CCWGG | 1 cut(s) | 265 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 102, 134 |
| AluBI | AGCT | 3 cut(s) | 55, 76, 309 |
| AluI | AGCT | 3 cut(s) | 55, 76, 309 |
| Ama87I | CYCGRG | 1 cut(s) | 56 |
| AsuHPI | GGTGA | 1 cut(s) | 281 |
| AvaI | CYCGRG | 1 cut(s) | 56 |
| BciT130I | CCWGG | 1 cut(s) | 267 |
| BfaI | CTAG | 2 cut(s) | 306, 310 |
| Bme1390I | CCNGG | 1 cut(s) | 267 |
| BmeT110I | CYCGRG | 1 cut(s) | 56 |
| BmgBI | CACGTC | 1 cut(s) | 263 |
| BmrFI | CCNGG | 1 cut(s) | 267 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 170, 202 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 134, 164, 277, 307 |
| Bse3DI | GCAATG | 1 cut(s) | 31 |
| BseBI | CCWGG | 1 cut(s) | 267 |
| BseMI | GCAATG | 1 cut(s) | 31 |
| BseMII | CTCAG | 1 cut(s) | 228 |
| BseRI | GAGGAG | 1 cut(s) | 116 |
| BsiHKCI | CYCGRG | 1 cut(s) | 56 |
| BsoBI | CYCGRG | 1 cut(s) | 56 |
| BspCNI | CTCAG | 1 cut(s) | 229 |
| BspQI | GCTCTTC | 1 cut(s) | 66 |
| BsrDI | GCAATG | 1 cut(s) | 31 |
| Bst2UI | CCWGG | 1 cut(s) | 267 |
| Bst6I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| BstDEI | CTNAG | 2 cut(s) | 77, 237 |
| BstNI | CCWGG | 1 cut(s) | 267 |
| BstSCI | CCNGG | 1 cut(s) | 265 |
| BtrI | CACGTC | 1 cut(s) | 263 |
| BtsIMutI | CAGTG | 1 cut(s) | 188 |
| CviJI | RGCY | 4 cut(s) | 55, 76, 185, 309 |
| CviKI_1 | RGCY | 4 cut(s) | 55, 76, 185, 309 |
| DdeI | CTNAG | 2 cut(s) | 77, 237 |
| Eam1104I | CTCTTC | 3 cut(s) | 24, 66, 129 |
| EarI | CTCTTC | 3 cut(s) | 24, 66, 129 |
| Eco88I | CYCGRG | 1 cut(s) | 56 |
| EcoRII | CCWGG | 1 cut(s) | 265 |
| FaiI | YATR | 3 cut(s) | 149, 221, 246 |
| FspBI | CTAG | 2 cut(s) | 306, 310 |
| HinfI | GANTC | 3 cut(s) | 193, 248, 281 |
| HphI | GGTGA | 1 cut(s) | 281 |
| HpyAV | CCTTC | 1 cut(s) | 133 |
| HpyCH4IV | ACGT | 1 cut(s) | 262 |
| HpyCH4V | TGCA | 1 cut(s) | 172 |
| HpyF3I | CTNAG | 2 cut(s) | 77, 237 |
| HpySE526I | ACGT | 1 cut(s) | 262 |
| LguI | GCTCTTC | 1 cut(s) | 66 |
| LpnPI | CCDG | 2 cut(s) | 252, 279 |
| MaeI | CTAG | 2 cut(s) | 306, 310 |
| MaeII | ACGT | 1 cut(s) | 262 |
| MboII | GAAGA | 5 cut(s) | 41, 46, 80, 83, 146 |
| MfeI | CAATTG | 1 cut(s) | 209 |
| MluCI | AATT | 4 cut(s) | 41, 117, 174, 209 |
| MlyI | GAGTC | 2 cut(s) | 257, 275 |
| MnlI | CCTC | 7 cut(s) | 52, 94, 136, 157, 196, 208, 303 |
| MseI | TTAA | 1 cut(s) | 233 |
| MslI | CAYNNNNRTG | 1 cut(s) | 224 |
| MspR9I | CCNGG | 1 cut(s) | 267 |
| MunI | CAATTG | 1 cut(s) | 209 |
| MvaI | CCWGG | 1 cut(s) | 267 |
| PaeR7I | CTCGAG | 1 cut(s) | 56 |
| PciSI | GCTCTTC | 1 cut(s) | 66 |
| PfeI | GAWTC | 1 cut(s) | 193 |
| PleI | GAGTC | 2 cut(s) | 256, 275 |
| PpsI | GAGTC | 2 cut(s) | 256, 275 |
| Psp6I | CCWGG | 1 cut(s) | 265 |
| PspGI | CCWGG | 1 cut(s) | 265 |
| PspXI | VCTCGAGB | 1 cut(s) | 56 |
| RseI | CAYNNNNRTG | 1 cut(s) | 224 |
| SapI | GCTCTTC | 1 cut(s) | 66 |
| SaqAI | TTAA | 1 cut(s) | 233 |
| SchI | GAGTC | 2 cut(s) | 257, 275 |
| ScrFI | CCNGG | 1 cut(s) | 267 |
| SetI | ASST | 8 cut(s) | 57, 78, 168, 265, 271, 295, 307, 311 |
| Sfr274I | CTCGAG | 1 cut(s) | 56 |
| SgeI | CNNG | 5 cut(s) | 68, 70, 273, 278, 279 |
| SlaI | CTCGAG | 1 cut(s) | 56 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 224 |
| SmlI | CTYRAG | 1 cut(s) | 56 |
| SmoI | CTYRAG | 1 cut(s) | 56 |
| Sse9I | AATT | 4 cut(s) | 41, 117, 174, 209 |
| SspMI | CTAG | 2 cut(s) | 306, 310 |
| StyD4I | CCNGG | 1 cut(s) | 265 |
| TaiI | ACGT | 1 cut(s) | 265 |
| TaqI | TCGA | 1 cut(s) | 57 |
| TasI | AATT | 4 cut(s) | 41, 117, 174, 209 |
| TfiI | GAWTC | 1 cut(s) | 193 |
| Tru1I | TTAA | 1 cut(s) | 233 |
| Tru9I | TTAA | 1 cut(s) | 233 |
| TscAI | CASTG | 1 cut(s) | 195 |
| TspDTI | ATGAA | 2 cut(s) | 42, 54 |
| TspRI | CASTG | 1 cut(s) | 195 |
| XhoI | CTCGAG | 1 cut(s) | 56 |
| XspI | CTAG | 2 cut(s) | 306, 310 |