function. Its presence in a non-photosynthetic plant (Epifagus virginiana) and experiments in tobacco indicate that it has an essential function which is probably not related to photosynthesis
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 195 |
| AclWI | GGATC | 3 cut(s) | 348, 359, 417 |
| AcoI | YGGCCR | 1 cut(s) | 196 |
| AcsI | RAATTY | 1 cut(s) | 292 |
| AgsI | TTSAA | 4 cut(s) | 44, 266, 361, 450 |
| AjnI | CCWGG | 1 cut(s) | 159 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 141, 173 |
| AluBI | AGCT | 1 cut(s) | 442 |
| AluI | AGCT | 1 cut(s) | 442 |
| Alw26I | GTCTC | 2 cut(s) | 72, 202 |
| AlwI | GGATC | 3 cut(s) | 348, 359, 417 |
| Ama87I | CYCGRG | 1 cut(s) | 134 |
| AoxI | GGCC | 1 cut(s) | 196 |
| ApeKI | GCWGC | 2 cut(s) | 251, 439 |
| ApoI | RAATTY | 1 cut(s) | 292 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 50, 82, 217, 249 |
| AspA2I | CCTAGG | 1 cut(s) | 368 |
| AspLEI | GCGC | 1 cut(s) | 256 |
| AspS9I | GGNCC | 1 cut(s) | 394 |
| AsuHPI | GGTGA | 4 cut(s) | 28, 257, 321, 398 |
| AsuII | TTCGAA | 1 cut(s) | 48 |
| AvaI | CYCGRG | 1 cut(s) | 134 |
| AvaII | GGWCC | 1 cut(s) | 394 |
| AvrII | CCTAGG | 1 cut(s) | 368 |
| BbsI | GAAGAC | 1 cut(s) | 396 |
| BbvI | GCAGC | 2 cut(s) | 263, 451 |
| BceAI | ACGGC | 2 cut(s) | 362, 461 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 217, 251 |
| BciT130I | CCWGG | 1 cut(s) | 161 |
| BcoDI | GTCTC | 2 cut(s) | 72, 202 |
| BfaI | CTAG | 1 cut(s) | 369 |
| BfoI | RGCGCY | 1 cut(s) | 257 |
| BisI | GCNGC | 3 cut(s) | 196, 252, 440 |
| BlnI | CCTAGG | 1 cut(s) | 368 |
| BlsI | GCNGC | 3 cut(s) | 197, 253, 441 |
| Bme1390I | CCNGG | 1 cut(s) | 161 |
| Bme18I | GGWCC | 1 cut(s) | 394 |
| BmeT110I | CYCGRG | 1 cut(s) | 134 |
| BmgT120I | GGNCC | 1 cut(s) | 394 |
| BmiI | GGNNCC | 1 cut(s) | 396 |
| BmrFI | CCNGG | 1 cut(s) | 161 |
| BmsI | GCATC | 1 cut(s) | 486 |
| BpiI | GAAGAC | 1 cut(s) | 396 |
| BpmI | CTGGAG | 1 cut(s) | 143 |
| Bpu14I | TTCGAA | 1 cut(s) | 48 |
| Bsa29I | ATCGAT | 1 cut(s) | 421 |
| BsaJI | CCNNGG | 5 cut(s) | 133, 160, 368, 408, 477 |
| BsaWI | WCCGGW | 1 cut(s) | 337 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 141, 171 |
| Bse3DI | GCAATG | 1 cut(s) | 69 |
| BseBI | CCWGG | 1 cut(s) | 161 |
| BseCI | ATCGAT | 1 cut(s) | 421 |
| BseDI | CCNNGG | 5 cut(s) | 133, 160, 368, 408, 477 |
| BseGI | GGATG | 2 cut(s) | 112, 177 |
| BseMI | GCAATG | 1 cut(s) | 69 |
| BseXI | GCAGC | 2 cut(s) | 263, 451 |
| Bsh1285I | CGRYCG | 2 cut(s) | 231, 338 |
| BshFI | GGCC | 1 cut(s) | 198 |
| BshVI | ATCGAT | 1 cut(s) | 421 |
| BsiEI | CGRYCG | 2 cut(s) | 231, 338 |
| BsiHKCI | CYCGRG | 1 cut(s) | 134 |
| BsiSI | CCGG | 3 cut(s) | 14, 192, 338 |
| BslFI | GGGAC | 1 cut(s) | 380 |
| BsmAI | GTCTC | 2 cut(s) | 72, 202 |
| BsmFI | GGGAC | 1 cut(s) | 380 |
| BsmI | GAATGC | 2 cut(s) | 379, 490 |
| BsnI | GGCC | 1 cut(s) | 198 |
| BsoBI | CYCGRG | 1 cut(s) | 134 |
| Bsp119I | TTCGAA | 1 cut(s) | 48 |
| Bsp143I | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| BspACI | CCGC | 1 cut(s) | 195 |
| BspANI | GGCC | 1 cut(s) | 198 |
| BspDI | ATCGAT | 1 cut(s) | 421 |
| BspHI | TCATGA | 1 cut(s) | 97 |
| BspLI | GGNNCC | 1 cut(s) | 396 |
| BspPI | GGATC | 3 cut(s) | 348, 359, 417 |
| BspT104I | TTCGAA | 1 cut(s) | 48 |
| BsrDI | GCAATG | 1 cut(s) | 69 |
| BssECI | CCNNGG | 5 cut(s) | 133, 160, 368, 408, 477 |
| BssMI | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| BssT1I | CCWWGG | 1 cut(s) | 368 |
| Bst2UI | CCWGG | 1 cut(s) | 161 |
| Bst4CI | ACNGT | 1 cut(s) | 245 |
| Bst6I | CTCTTC | 2 cut(s) | 188, 267 |
| BstBI | TTCGAA | 1 cut(s) | 48 |
| BstC8I | GCNNGC | 1 cut(s) | 490 |
| BstDSI | CCRYGG | 1 cut(s) | 477 |
| BstF5I | GGATG | 2 cut(s) | 112, 177 |
| BstH2I | RGCGCY | 1 cut(s) | 257 |
| BstHHI | GCGC | 1 cut(s) | 256 |
| BstKTI | GATC | 6 cut(s) | 103, 231, 343, 367, 425, 503 |
| BstMAI | GTCTC | 2 cut(s) | 72, 202 |
| BstMBI | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| BstMCI | CGRYCG | 2 cut(s) | 231, 338 |
| BstNI | CCWGG | 1 cut(s) | 161 |
| BstSCI | CCNGG | 1 cut(s) | 159 |
| BstV1I | GCAGC | 2 cut(s) | 263, 451 |
| BstV2I | GAAGAC | 1 cut(s) | 396 |
| BstX2I | RGATCY | 2 cut(s) | 340, 364 |
| BstYI | RGATCY | 2 cut(s) | 340, 364 |
| Bsu15I | ATCGAT | 1 cut(s) | 421 |
| BsuRI | GGCC | 1 cut(s) | 198 |
| BsuTUI | ATCGAT | 1 cut(s) | 421 |
| BtgI | CCRYGG | 1 cut(s) | 477 |
| BtsCI | GGATG | 2 cut(s) | 112, 177 |
| Cac8I | GCNNGC | 1 cut(s) | 490 |
| CciI | TCATGA | 1 cut(s) | 97 |
| CfoI | GCGC | 1 cut(s) | 256 |
| Cfr13I | GGNCC | 1 cut(s) | 394 |
| ClaI | ATCGAT | 1 cut(s) | 421 |
| CviAII | CATG | 1 cut(s) | 98 |
| CviJI | RGCY | 3 cut(s) | 198, 442, 476 |
| CviKI_1 | RGCY | 3 cut(s) | 198, 442, 476 |
| DpnI | GATC | 6 cut(s) | 102, 230, 342, 366, 424, 502 |
| DpnII | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| EaeI | YGGCCR | 1 cut(s) | 196 |
| Eam1104I | CTCTTC | 2 cut(s) | 188, 267 |
| EarI | CTCTTC | 2 cut(s) | 188, 267 |
| Eco130I | CCWWGG | 1 cut(s) | 368 |
| Eco47I | GGWCC | 1 cut(s) | 394 |
| Eco88I | CYCGRG | 1 cut(s) | 134 |
| EcoRII | CCWGG | 1 cut(s) | 159 |
| EcoT14I | CCWWGG | 1 cut(s) | 368 |
| ErhI | CCWWGG | 1 cut(s) | 368 |
| FaeI | CATG | 1 cut(s) | 101 |
| FaiI | YATR | 6 cut(s) | 8, 33, 77, 82, 99, 215 |
| FaqI | GGGAC | 1 cut(s) | 380 |
| FatI | CATG | 1 cut(s) | 97 |
| Fnu4HI | GCNGC | 3 cut(s) | 196, 252, 440 |
| FokI | GGATG | 2 cut(s) | 119, 164 |
| Fsp4HI | GCNGC | 3 cut(s) | 196, 252, 440 |
| FspBI | CTAG | 1 cut(s) | 369 |
| GlaI | GCGC | 1 cut(s) | 255 |
| GluI | GCNGC | 3 cut(s) | 196, 252, 440 |
| GsuI | CTGGAG | 1 cut(s) | 143 |
| HaeII | RGCGCY | 1 cut(s) | 257 |
| HaeIII | GGCC | 1 cut(s) | 198 |
| HapII | CCGG | 3 cut(s) | 14, 192, 338 |
| HhaI | GCGC | 1 cut(s) | 256 |
| Hin1II | CATG | 1 cut(s) | 101 |
| Hin6I | GCGC | 1 cut(s) | 254 |
| HinP1I | GCGC | 1 cut(s) | 254 |
| HinfI | GANTC | 5 cut(s) | 85, 116, 259, 302, 323 |
| HpaII | CCGG | 3 cut(s) | 14, 192, 338 |
| HphI | GGTGA | 4 cut(s) | 28, 257, 321, 398 |
| Hpy166II | GTNNAC | 1 cut(s) | 149 |
| Hpy188I | TCNGA | 4 cut(s) | 207, 225, 409, 427 |
| Hpy188III | TCNNGA | 3 cut(s) | 98, 104, 344 |
| Hpy8I | GTNNAC | 1 cut(s) | 149 |
| Hpy99I | CGWCG | 1 cut(s) | 229 |
| HpyAV | CCTTC | 3 cut(s) | 50, 520, 534 |
| HpyCH4III | ACNGT | 1 cut(s) | 245 |
| HpyCH4IV | ACGT | 1 cut(s) | 217 |
| HpyCH4V | TGCA | 1 cut(s) | 75 |
| HpySE526I | ACGT | 1 cut(s) | 217 |
| Hsp92II | CATG | 1 cut(s) | 101 |
| HspAI | GCGC | 1 cut(s) | 254 |
| Kzo9I | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| LpnPI | CCDG | 7 cut(s) | 27, 89, 146, 173, 205, 351, 449 |
| Lsp1109I | GCAGC | 2 cut(s) | 263, 451 |
| LweI | GCATC | 1 cut(s) | 486 |
| MaeI | CTAG | 1 cut(s) | 369 |
| MaeII | ACGT | 1 cut(s) | 217 |
| MaeIII | GTNAC | 1 cut(s) | 327 |
| MalI | GATC | 6 cut(s) | 102, 230, 342, 366, 424, 502 |
| MboI | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| MboII | GAAGA | 4 cut(s) | 175, 254, 396, 549 |
| MflI | RGATCY | 2 cut(s) | 340, 364 |
| MluCI | AATT | 7 cut(s) | 44, 50, 121, 170, 292, 310, 462 |
| MlyI | GAGTC | 2 cut(s) | 125, 253 |
| MmeI | TCCRAC | 3 cut(s) | 248, 450, 538 |
| MnlI | CCTC | 5 cut(s) | 143, 191, 342, 403, 516 |
| MseI | TTAA | 3 cut(s) | 167, 465, 562 |
| MspI | CCGG | 3 cut(s) | 14, 192, 338 |
| MspR9I | CCNGG | 1 cut(s) | 161 |
| Mva1269I | GAATGC | 2 cut(s) | 379, 490 |
| MvaI | CCWGG | 1 cut(s) | 161 |
| NdeII | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| NlaIII | CATG | 1 cut(s) | 101 |
| NlaIV | GGNNCC | 1 cut(s) | 396 |
| NmuCI | GTSAC | 1 cut(s) | 327 |
| NspV | TTCGAA | 1 cut(s) | 48 |
| PagI | TCATGA | 1 cut(s) | 97 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 496 |
| PctI | GAATGC | 2 cut(s) | 379, 490 |
| PfeI | GAWTC | 3 cut(s) | 85, 302, 323 |
| PkrI | GCNGC | 3 cut(s) | 197, 253, 441 |
| Ple19I | CGATCG | 1 cut(s) | 231 |
| PleI | GAGTC | 2 cut(s) | 124, 253 |
| PpsI | GAGTC | 2 cut(s) | 124, 253 |
| Psp6I | CCWGG | 1 cut(s) | 159 |
| PspGI | CCWGG | 1 cut(s) | 159 |
| PspN4I | GGNNCC | 1 cut(s) | 396 |
| PspPI | GGNCC | 1 cut(s) | 394 |
| PsuI | RGATCY | 2 cut(s) | 340, 364 |
| PvuI | CGATCG | 1 cut(s) | 231 |
| SaqAI | TTAA | 3 cut(s) | 167, 465, 562 |
| SatI | GCNGC | 3 cut(s) | 196, 252, 440 |
| Sau3AI | GATC | 6 cut(s) | 100, 228, 340, 364, 422, 500 |
| Sau96I | GGNCC | 1 cut(s) | 394 |
| SchI | GAGTC | 2 cut(s) | 125, 253 |
| ScrFI | CCNGG | 1 cut(s) | 161 |
| SetI | ASST | 4 cut(s) | 220, 334, 388, 444 |
| SfaNI | GCATC | 1 cut(s) | 486 |
| SfuI | TTCGAA | 1 cut(s) | 48 |
| SinI | GGWCC | 1 cut(s) | 394 |
| Sse9I | AATT | 7 cut(s) | 44, 50, 121, 170, 292, 310, 462 |
| SsiI | CCGC | 1 cut(s) | 195 |
| SspMI | CTAG | 1 cut(s) | 369 |
| StyD4I | CCNGG | 1 cut(s) | 159 |
| StyI | CCWWGG | 1 cut(s) | 368 |
| TaaI | ACNGT | 1 cut(s) | 245 |
| TaiI | ACGT | 1 cut(s) | 220 |
| TaqI | TCGA | 6 cut(s) | 48, 119, 321, 334, 345, 421 |
| TaqII | GACCGA | 1 cut(s) | 39 |
| TasI | AATT | 7 cut(s) | 44, 50, 121, 170, 292, 310, 462 |
| TauI | GCSGC | 1 cut(s) | 198 |
| TfiI | GAWTC | 3 cut(s) | 85, 302, 323 |
| Tru1I | TTAA | 3 cut(s) | 167, 465, 562 |
| Tru9I | TTAA | 3 cut(s) | 167, 465, 562 |
| TseFI | GTSAC | 1 cut(s) | 327 |
| TseI | GCWGC | 2 cut(s) | 251, 439 |
| Tsp45I | GTSAC | 1 cut(s) | 327 |
| TspDTI | ATGAA | 3 cut(s) | 86, 166, 175 |
| VpaK11BI | GGWCC | 1 cut(s) | 394 |
| XapI | RAATTY | 1 cut(s) | 292 |
| XmaJI | CCTAGG | 1 cut(s) | 368 |
| XspI | CTAG | 1 cut(s) | 369 |