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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcuI | CTGAAG | 1 cut(s) | 200 |
| AgsI | TTSAA | 2 cut(s) | 140, 430 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 248, 280, 444, 476 |
| AluBI | AGCT | 2 cut(s) | 235, 397 |
| AluI | AGCT | 2 cut(s) | 235, 397 |
| Alw26I | GTCTC | 3 cut(s) | 164, 200, 341 |
| AsuHPI | GGTGA | 2 cut(s) | 175, 332 |
| BccI | CCATC | 2 cut(s) | 113, 337 |
| BcoDI | GTCTC | 3 cut(s) | 164, 200, 341 |
| BfaI | CTAG | 1 cut(s) | 144 |
| BmsI | GCATC | 2 cut(s) | 49, 185 |
| BpuEI | CTTGAG | 1 cut(s) | 419 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 476, 506 |
| Bse1I | ACTGG | 2 cut(s) | 150, 303 |
| BseMII | CTCAG | 1 cut(s) | 69 |
| BseNI | ACTGG | 2 cut(s) | 150, 303 |
| BseRI | GAGGAG | 1 cut(s) | 458 |
| BsmAI | GTCTC | 3 cut(s) | 164, 200, 341 |
| BspCNI | CTCAG | 1 cut(s) | 70 |
| BspQI | GCTCTTC | 1 cut(s) | 225 |
| BsrI | ACTGG | 2 cut(s) | 150, 303 |
| Bst4CI | ACNGT | 2 cut(s) | 9, 329 |
| Bst6I | CTCTTC | 4 cut(s) | 225, 366, 408, 471 |
| BstDEI | CTNAG | 2 cut(s) | 78, 236 |
| BstMAI | GTCTC | 3 cut(s) | 164, 200, 341 |
| BtsIMutI | CAGTG | 2 cut(s) | 5, 157 |
| CviJI | RGCY | 2 cut(s) | 235, 397 |
| CviKI_1 | RGCY | 2 cut(s) | 235, 397 |
| DdeI | CTNAG | 2 cut(s) | 78, 236 |
| Eam1104I | CTCTTC | 4 cut(s) | 225, 366, 408, 471 |
| EarI | CTCTTC | 4 cut(s) | 225, 366, 408, 471 |
| Eco57I | CTGAAG | 1 cut(s) | 200 |
| FaiI | YATR | 2 cut(s) | 161, 491 |
| FspBI | CTAG | 1 cut(s) | 144 |
| HincII | GTYRAC | 1 cut(s) | 12 |
| HindII | GTYRAC | 1 cut(s) | 12 |
| HindIII | AAGCTT | 1 cut(s) | 395 |
| HinfI | GANTC | 2 cut(s) | 74, 80 |
| HphI | GGTGA | 2 cut(s) | 175, 332 |
| Hpy166II | GTNNAC | 1 cut(s) | 12 |
| Hpy188I | TCNGA | 3 cut(s) | 33, 79, 219 |
| Hpy8I | GTNNAC | 1 cut(s) | 12 |
| HpyAV | CCTTC | 2 cut(s) | 160, 475 |
| HpyCH4III | ACNGT | 2 cut(s) | 9, 329 |
| HpyCH4V | TGCA | 3 cut(s) | 62, 176, 514 |
| HpyF3I | CTNAG | 2 cut(s) | 78, 236 |
| LguI | GCTCTTC | 1 cut(s) | 225 |
| LpnPI | CCDG | 2 cut(s) | 163, 284 |
| LweI | GCATC | 2 cut(s) | 49, 185 |
| MaeI | CTAG | 1 cut(s) | 144 |
| MaeIII | GTNAC | 3 cut(s) | 3, 320, 329 |
| MluCI | AATT | 6 cut(s) | 21, 200, 284, 383, 459, 516 |
| MlyI | GAGTC | 1 cut(s) | 89 |
| MnlI | CCTC | 7 cut(s) | 58, 235, 394, 436, 478, 499, 514 |
| MseI | TTAA | 1 cut(s) | 51 |
| NmuCI | GTSAC | 2 cut(s) | 3, 320 |
| PciSI | GCTCTTC | 1 cut(s) | 225 |
| PfeI | GAWTC | 1 cut(s) | 74 |
| PleI | GAGTC | 1 cut(s) | 88 |
| PpsI | GAGTC | 1 cut(s) | 88 |
| SapI | GCTCTTC | 1 cut(s) | 225 |
| SaqAI | TTAA | 1 cut(s) | 51 |
| SchI | GAGTC | 1 cut(s) | 89 |
| SetI | ASST | 5 cut(s) | 237, 246, 322, 399, 510 |
| SfaNI | GCATC | 2 cut(s) | 49, 185 |
| SgeI | CNNG | 7 cut(s) | 137, 156, 162, 277, 311, 318, 410 |
| SmlI | CTYRAG | 1 cut(s) | 398 |
| SmoI | CTYRAG | 1 cut(s) | 398 |
| Sse9I | AATT | 6 cut(s) | 21, 200, 284, 383, 459, 516 |
| SspMI | CTAG | 1 cut(s) | 144 |
| TaaI | ACNGT | 2 cut(s) | 9, 329 |
| TaqI | TCGA | 1 cut(s) | 179 |
| TaqII | GACCGA | 1 cut(s) | 380 |
| TasI | AATT | 6 cut(s) | 21, 200, 284, 383, 459, 516 |
| TfiI | GAWTC | 1 cut(s) | 74 |
| Tru1I | TTAA | 1 cut(s) | 51 |
| Tru9I | TTAA | 1 cut(s) | 51 |
| TscAI | CASTG | 2 cut(s) | 12, 157 |
| TseFI | GTSAC | 2 cut(s) | 3, 320 |
| Tsp45I | GTSAC | 2 cut(s) | 3, 320 |
| TspDTI | ATGAA | 2 cut(s) | 384, 396 |
| TspRI | CASTG | 2 cut(s) | 12, 157 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 157 |
| XspI | CTAG | 1 cut(s) | 144 |