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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 140 |
| AciI | CCGC | 3 cut(s) | 140, 158, 344 |
| AclWI | GGATC | 1 cut(s) | 46 |
| AcsI | RAATTY | 2 cut(s) | 134, 190 |
| AflIII | ACRYGT | 1 cut(s) | 98 |
| AgsI | TTSAA | 3 cut(s) | 21, 214, 235 |
| AluBI | AGCT | 1 cut(s) | 284 |
| AluI | AGCT | 1 cut(s) | 284 |
| AlwI | GGATC | 1 cut(s) | 46 |
| ApeKI | GCWGC | 2 cut(s) | 281, 355 |
| ApoI | RAATTY | 2 cut(s) | 134, 190 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 175, 207 |
| AsuHPI | GGTGA | 1 cut(s) | 267 |
| BanII | GRGCYC | 1 cut(s) | 260 |
| BauI | CACGAG | 1 cut(s) | 293 |
| BbvI | GCAGC | 2 cut(s) | 293, 342 |
| BfaI | CTAG | 1 cut(s) | 15 |
| BisI | GCNGC | 2 cut(s) | 282, 356 |
| BlsI | GCNGC | 2 cut(s) | 283, 357 |
| BmsI | GCATC | 1 cut(s) | 212 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 236, 266 |
| Bse118I | RCCGGY | 1 cut(s) | 277 |
| Bse1I | ACTGG | 1 cut(s) | 58 |
| BseNI | ACTGG | 1 cut(s) | 58 |
| BseXI | GCAGC | 2 cut(s) | 293, 342 |
| Bsh1236I | CGCG | 1 cut(s) | 140 |
| BsiSI | CCGG | 1 cut(s) | 278 |
| BslFI | GGGAC | 1 cut(s) | 283 |
| BsmFI | GGGAC | 1 cut(s) | 283 |
| BsmI | GAATGC | 1 cut(s) | 160 |
| Bsp1286I | GDGCHC | 1 cut(s) | 260 |
| Bsp143I | GATC | 1 cut(s) | 38 |
| BspACI | CCGC | 3 cut(s) | 140, 158, 344 |
| BspFNI | CGCG | 1 cut(s) | 140 |
| BspPI | GGATC | 1 cut(s) | 46 |
| BspQI | GCTCTTC | 1 cut(s) | 315 |
| BsrFI | RCCGGY | 1 cut(s) | 277 |
| BsrI | ACTGG | 1 cut(s) | 58 |
| BssAI | RCCGGY | 1 cut(s) | 277 |
| BssMI | GATC | 1 cut(s) | 38 |
| BssSI | CACGAG | 1 cut(s) | 293 |
| Bst2BI | CACGAG | 1 cut(s) | 293 |
| Bst6I | CTCTTC | 1 cut(s) | 315 |
| BstC8I | GCNNGC | 1 cut(s) | 12 |
| BstFNI | CGCG | 1 cut(s) | 140 |
| BstKTI | GATC | 1 cut(s) | 41 |
| BstMBI | GATC | 1 cut(s) | 38 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 352 |
| BstNSI | RCATGY | 2 cut(s) | 102, 350 |
| BstUI | CGCG | 1 cut(s) | 140 |
| BstV1I | GCAGC | 2 cut(s) | 293, 342 |
| Cac8I | GCNNGC | 1 cut(s) | 12 |
| Cfr10I | RCCGGY | 1 cut(s) | 277 |
| CviAII | CATG | 6 cut(s) | 59, 71, 99, 110, 148, 347 |
| CviJI | RGCY | 3 cut(s) | 258, 284, 355 |
| CviKI_1 | RGCY | 3 cut(s) | 258, 284, 355 |
| DpnI | GATC | 1 cut(s) | 40 |
| DpnII | GATC | 1 cut(s) | 38 |
| Eam1104I | CTCTTC | 1 cut(s) | 315 |
| EarI | CTCTTC | 1 cut(s) | 315 |
| Eco24I | GRGCYC | 1 cut(s) | 260 |
| EcoT38I | GRGCYC | 1 cut(s) | 260 |
| FaeI | CATG | 6 cut(s) | 62, 74, 102, 113, 151, 350 |
| FaqI | GGGAC | 1 cut(s) | 283 |
| FatI | CATG | 6 cut(s) | 58, 70, 98, 109, 147, 346 |
| Fnu4HI | GCNGC | 2 cut(s) | 282, 356 |
| FriOI | GRGCYC | 1 cut(s) | 260 |
| Fsp4HI | GCNGC | 2 cut(s) | 282, 356 |
| FspBI | CTAG | 1 cut(s) | 15 |
| GluI | GCNGC | 2 cut(s) | 282, 356 |
| HapII | CCGG | 1 cut(s) | 278 |
| Hin1II | CATG | 6 cut(s) | 62, 74, 102, 113, 151, 350 |
| HinfI | GANTC | 3 cut(s) | 265, 296, 332 |
| HpaII | CCGG | 1 cut(s) | 278 |
| HphI | GGTGA | 1 cut(s) | 267 |
| HpyAV | CCTTC | 1 cut(s) | 208 |
| HpyCH4V | TGCA | 2 cut(s) | 203, 341 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 352 |
| Hsp92II | CATG | 6 cut(s) | 62, 74, 102, 113, 151, 350 |
| Kzo9I | GATC | 1 cut(s) | 38 |
| LguI | GCTCTTC | 1 cut(s) | 315 |
| LmnI | GCTCC | 1 cut(s) | 244 |
| LpnPI | CCDG | 3 cut(s) | 39, 107, 291 |
| Lsp1109I | GCAGC | 2 cut(s) | 293, 342 |
| LweI | GCATC | 1 cut(s) | 212 |
| MaeI | CTAG | 1 cut(s) | 15 |
| MaeIII | GTNAC | 5 cut(s) | 61, 73, 89, 101, 181 |
| MalI | GATC | 1 cut(s) | 40 |
| MboI | GATC | 1 cut(s) | 38 |
| MboII | GAAGA | 2 cut(s) | 247, 332 |
| MhlI | GDGCHC | 1 cut(s) | 260 |
| MluCI | AATT | 4 cut(s) | 134, 190, 230, 239 |
| MlyI | GAGTC | 2 cut(s) | 259, 305 |
| MnlI | CCTC | 4 cut(s) | 136, 154, 217, 283 |
| MspI | CCGG | 1 cut(s) | 278 |
| Mva1269I | GAATGC | 1 cut(s) | 160 |
| MvnI | CGCG | 1 cut(s) | 140 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 352 |
| NdeII | GATC | 1 cut(s) | 38 |
| NlaIII | CATG | 6 cut(s) | 62, 74, 102, 113, 151, 350 |
| NmuCI | GTSAC | 1 cut(s) | 181 |
| NspI | RCATGY | 2 cut(s) | 102, 350 |
| PciI | ACATGT | 1 cut(s) | 98 |
| PciSI | GCTCTTC | 1 cut(s) | 315 |
| PctI | GAATGC | 1 cut(s) | 160 |
| PfeI | GAWTC | 1 cut(s) | 332 |
| PkrI | GCNGC | 2 cut(s) | 283, 357 |
| PleI | GAGTC | 2 cut(s) | 259, 304 |
| PpsI | GAGTC | 2 cut(s) | 259, 304 |
| PscI | ACATGT | 1 cut(s) | 98 |
| SapI | GCTCTTC | 1 cut(s) | 315 |
| SatI | GCNGC | 2 cut(s) | 282, 356 |
| Sau3AI | GATC | 1 cut(s) | 38 |
| SchI | GAGTC | 2 cut(s) | 259, 305 |
| SduI | GDGCHC | 1 cut(s) | 260 |
| SetI | ASST | 3 cut(s) | 275, 286, 310 |
| SfaNI | GCATC | 1 cut(s) | 212 |
| Sse9I | AATT | 4 cut(s) | 134, 190, 230, 239 |
| SsiI | CCGC | 3 cut(s) | 140, 158, 344 |
| SspMI | CTAG | 1 cut(s) | 15 |
| TasI | AATT | 4 cut(s) | 134, 190, 230, 239 |
| TfiI | GAWTC | 1 cut(s) | 332 |
| TseFI | GTSAC | 1 cut(s) | 181 |
| TseI | GCWGC | 2 cut(s) | 281, 355 |
| Tsp45I | GTSAC | 1 cut(s) | 181 |
| TspDTI | ATGAA | 3 cut(s) | 63, 126, 345 |
| XapI | RAATTY | 2 cut(s) | 134, 190 |
| XceI | RCATGY | 2 cut(s) | 102, 350 |
| XspI | CTAG | 1 cut(s) | 15 |