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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 623 |
| AccIII | TCCGGA | 1 cut(s) | 35 |
| AclWI | GGATC | 1 cut(s) | 488 |
| AcsI | RAATTY | 2 cut(s) | 460, 888 |
| AcuI | CTGAAG | 5 cut(s) | 72, 165, 207, 312, 417 |
| AfaI | GTAC | 1 cut(s) | 585 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 539, 623 |
| AgsI | TTSAA | 3 cut(s) | 194, 511, 592 |
| AjiI | CACGTC | 1 cut(s) | 761 |
| AjnI | CCWGG | 1 cut(s) | 763 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 60, 92, 503, 535, 622, 654 |
| AluBI | AGCT | 2 cut(s) | 506, 690 |
| AluI | AGCT | 2 cut(s) | 506, 690 |
| AlwI | GGATC | 1 cut(s) | 488 |
| Ama87I | CYCGRG | 1 cut(s) | 879 |
| Aor13HI | TCCGGA | 1 cut(s) | 35 |
| AoxI | GGCC | 1 cut(s) | 4 |
| ApeKI | GCWGC | 2 cut(s) | 491, 494 |
| ApoI | RAATTY | 2 cut(s) | 460, 888 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 596 |
| AspS9I | GGNCC | 2 cut(s) | 4, 829 |
| AsuC2I | CCSGG | 1 cut(s) | 221 |
| AsuHPI | GGTGA | 2 cut(s) | 308, 779 |
| AvaI | CYCGRG | 1 cut(s) | 879 |
| AvaII | GGWCC | 1 cut(s) | 829 |
| AxyI | CCTNAGG | 1 cut(s) | 834 |
| BbsI | GAAGAC | 1 cut(s) | 236 |
| BbvI | GCAGC | 2 cut(s) | 478, 481 |
| BccI | CCATC | 2 cut(s) | 413, 442 |
| BciT130I | CCWGG | 1 cut(s) | 765 |
| BcnI | CCSGG | 1 cut(s) | 221 |
| BfaI | CTAG | 2 cut(s) | 677, 804 |
| BfmI | CTRYAG | 1 cut(s) | 495 |
| BisI | GCNGC | 2 cut(s) | 492, 495 |
| BlsI | GCNGC | 2 cut(s) | 493, 496 |
| BmcAI | AGTACT | 1 cut(s) | 585 |
| Bme1390I | CCNGG | 2 cut(s) | 221, 765 |
| Bme18I | GGWCC | 1 cut(s) | 829 |
| BmeT110I | CYCGRG | 1 cut(s) | 879 |
| BmgBI | CACGTC | 1 cut(s) | 761 |
| BmgT120I | GGNCC | 2 cut(s) | 4, 829 |
| BmiI | GGNNCC | 1 cut(s) | 831 |
| BmrFI | CCNGG | 2 cut(s) | 221, 765 |
| BpiI | GAAGAC | 1 cut(s) | 236 |
| BpmI | CTGGAG | 2 cut(s) | 516, 645 |
| BpuMI | CCSGG | 1 cut(s) | 221 |
| BsaJI | CCNNGG | 1 cut(s) | 220 |
| BsaWI | WCCGGW | 1 cut(s) | 35 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 414, 444, 775, 805 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 539, 623 |
| Bse1I | ACTGG | 2 cut(s) | 262, 285 |
| Bse21I | CCTNAGG | 1 cut(s) | 834 |
| BseAI | TCCGGA | 1 cut(s) | 35 |
| BseBI | CCWGG | 1 cut(s) | 765 |
| BseDI | CCNNGG | 1 cut(s) | 220 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 539, 623 |
| BseMII | CTCAG | 2 cut(s) | 726, 848 |
| BseNI | ACTGG | 2 cut(s) | 262, 285 |
| BseRI | GAGGAG | 2 cut(s) | 777, 780 |
| BseXI | GCAGC | 2 cut(s) | 478, 481 |
| BseYI | CCCAGC | 1 cut(s) | 690 |
| BshFI | GGCC | 1 cut(s) | 6 |
| BsiHKCI | CYCGRG | 1 cut(s) | 879 |
| BsiSI | CCGG | 2 cut(s) | 36, 220 |
| BslFI | GGGAC | 3 cut(s) | 575, 656, 815 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 539, 623 |
| BsmFI | GGGAC | 3 cut(s) | 575, 656, 815 |
| BsnI | GGCC | 1 cut(s) | 6 |
| BsoBI | CYCGRG | 1 cut(s) | 879 |
| Bsp13I | TCCGGA | 1 cut(s) | 35 |
| Bsp143I | GATC | 1 cut(s) | 480 |
| BspANI | GGCC | 1 cut(s) | 6 |
| BspCNI | CTCAG | 2 cut(s) | 727, 847 |
| BspEI | TCCGGA | 1 cut(s) | 35 |
| BspLI | GGNNCC | 1 cut(s) | 831 |
| BspMAI | CTGCAG | 1 cut(s) | 499 |
| BspPI | GGATC | 1 cut(s) | 488 |
| BsrI | ACTGG | 2 cut(s) | 262, 285 |
| BssECI | CCNNGG | 1 cut(s) | 220 |
| BssMI | GATC | 1 cut(s) | 480 |
| Bst2UI | CCWGG | 1 cut(s) | 765 |
| BstC8I | GCNNGC | 1 cut(s) | 857 |
| BstDEI | CTNAG | 3 cut(s) | 234, 735, 834 |
| BstKTI | GATC | 1 cut(s) | 483 |
| BstMBI | GATC | 1 cut(s) | 480 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 503 |
| BstNI | CCWGG | 1 cut(s) | 765 |
| BstSCI | CCNGG | 2 cut(s) | 219, 763 |
| BstSFI | CTRYAG | 1 cut(s) | 495 |
| BstV1I | GCAGC | 2 cut(s) | 478, 481 |
| BstV2I | GAAGAC | 1 cut(s) | 236 |
| Bsu36I | CCTNAGG | 1 cut(s) | 834 |
| BsuRI | GGCC | 1 cut(s) | 6 |
| BtrI | CACGTC | 1 cut(s) | 761 |
| Cac8I | GCNNGC | 1 cut(s) | 857 |
| Cfr13I | GGNCC | 2 cut(s) | 4, 829 |
| Csp6I | GTAC | 1 cut(s) | 584 |
| CviJI | RGCY | 5 cut(s) | 6, 491, 506, 690, 844 |
| CviKI_1 | RGCY | 5 cut(s) | 6, 491, 506, 690, 844 |
| CviQI | GTAC | 1 cut(s) | 584 |
| DdeI | CTNAG | 3 cut(s) | 234, 735, 834 |
| DpnI | GATC | 1 cut(s) | 482 |
| DpnII | GATC | 1 cut(s) | 480 |
| Eco47I | GGWCC | 1 cut(s) | 829 |
| Eco57I | CTGAAG | 5 cut(s) | 72, 165, 207, 312, 417 |
| Eco81I | CCTNAGG | 1 cut(s) | 834 |
| Eco88I | CYCGRG | 1 cut(s) | 879 |
| EcoO109I | RGGNCCY | 1 cut(s) | 829 |
| EcoRII | CCWGG | 1 cut(s) | 763 |
| FaiI | YATR | 5 cut(s) | 648, 650, 687, 719, 744 |
| FaqI | GGGAC | 3 cut(s) | 575, 656, 815 |
| Fnu4HI | GCNGC | 2 cut(s) | 492, 495 |
| Fsp4HI | GCNGC | 2 cut(s) | 492, 495 |
| FspBI | CTAG | 2 cut(s) | 677, 804 |
| GluI | GCNGC | 2 cut(s) | 492, 495 |
| GsaI | CCCAGC | 1 cut(s) | 694 |
| GsuI | CTGGAG | 2 cut(s) | 516, 645 |
| HaeIII | GGCC | 1 cut(s) | 6 |
| HapII | CCGG | 2 cut(s) | 36, 220 |
| HincII | GTYRAC | 1 cut(s) | 473 |
| HindII | GTYRAC | 1 cut(s) | 473 |
| HindIII | AAGCTT | 1 cut(s) | 504 |
| HinfI | GANTC | 6 cut(s) | 32, 158, 200, 305, 654, 746 |
| HpaII | CCGG | 2 cut(s) | 36, 220 |
| HphI | GGTGA | 2 cut(s) | 308, 779 |
| Hpy166II | GTNNAC | 1 cut(s) | 473 |
| Hpy188I | TCNGA | 3 cut(s) | 52, 418, 466 |
| Hpy188III | TCNNGA | 8 cut(s) | 36, 65, 533, 589, 624, 677, 836, 879 |
| Hpy8I | GTNNAC | 1 cut(s) | 473 |
| HpyAV | CCTTC | 4 cut(s) | 232, 278, 287, 508 |
| HpyCH4IV | ACGT | 1 cut(s) | 760 |
| HpyCH4V | TGCA | 1 cut(s) | 497 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 503 |
| HpyF3I | CTNAG | 3 cut(s) | 234, 735, 834 |
| HpySE526I | ACGT | 1 cut(s) | 760 |
| Kpn2I | TCCGGA | 1 cut(s) | 35 |
| Kzo9I | GATC | 1 cut(s) | 480 |
| Lsp1109I | GCAGC | 2 cut(s) | 478, 481 |
| MaeI | CTAG | 2 cut(s) | 677, 804 |
| MaeII | ACGT | 1 cut(s) | 760 |
| MalI | GATC | 1 cut(s) | 482 |
| MboI | GATC | 1 cut(s) | 480 |
| MfeI | CAATTG | 2 cut(s) | 75, 707 |
| MluCI | AATT | 4 cut(s) | 75, 460, 707, 888 |
| MlyI | GAGTC | 2 cut(s) | 663, 755 |
| MroI | TCCGGA | 1 cut(s) | 35 |
| MroXI | GAANNNNTTC | 1 cut(s) | 596 |
| MslI | CAYNNNNRTG | 1 cut(s) | 722 |
| MspI | CCGG | 2 cut(s) | 36, 220 |
| MspR9I | CCNGG | 2 cut(s) | 221, 765 |
| MunI | CAATTG | 2 cut(s) | 75, 707 |
| MvaI | CCWGG | 1 cut(s) | 765 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 503 |
| NciI | CCSGG | 1 cut(s) | 221 |
| NdeII | GATC | 1 cut(s) | 480 |
| NlaIV | GGNNCC | 1 cut(s) | 831 |
| PaeR7I | CTCGAG | 1 cut(s) | 879 |
| PdmI | GAANNNNTTC | 1 cut(s) | 596 |
| PfeI | GAWTC | 4 cut(s) | 32, 158, 200, 305 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 623 |
| PkrI | GCNGC | 2 cut(s) | 493, 496 |
| PleI | GAGTC | 2 cut(s) | 662, 754 |
| PpsI | GAGTC | 2 cut(s) | 662, 754 |
| PpuMI | RGGWCCY | 1 cut(s) | 829 |
| Psp5II | RGGWCCY | 1 cut(s) | 829 |
| Psp6I | CCWGG | 1 cut(s) | 763 |
| PspFI | CCCAGC | 1 cut(s) | 690 |
| PspGI | CCWGG | 1 cut(s) | 763 |
| PspN4I | GGNNCC | 1 cut(s) | 831 |
| PspPI | GGNCC | 2 cut(s) | 4, 829 |
| PspPPI | RGGWCCY | 1 cut(s) | 829 |
| PstI | CTGCAG | 1 cut(s) | 499 |
| RsaI | GTAC | 1 cut(s) | 585 |
| RsaNI | GTAC | 1 cut(s) | 584 |
| RseI | CAYNNNNRTG | 1 cut(s) | 722 |
| SatI | GCNGC | 2 cut(s) | 492, 495 |
| Sau3AI | GATC | 1 cut(s) | 480 |
| Sau96I | GGNCC | 2 cut(s) | 4, 829 |
| ScaI | AGTACT | 1 cut(s) | 585 |
| SchI | GAGTC | 2 cut(s) | 663, 755 |
| ScrFI | CCNGG | 2 cut(s) | 221, 765 |
| SetI | ASST | 9 cut(s) | 298, 508, 597, 692, 763, 769, 805, 831, 886 |
| SfcI | CTRYAG | 1 cut(s) | 495 |
| Sfr274I | CTCGAG | 1 cut(s) | 879 |
| SinI | GGWCC | 1 cut(s) | 829 |
| SlaI | CTCGAG | 1 cut(s) | 879 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 722 |
| SmlI | CTYRAG | 1 cut(s) | 879 |
| SmoI | CTYRAG | 1 cut(s) | 879 |
| Sse9I | AATT | 4 cut(s) | 75, 460, 707, 888 |
| SspMI | CTAG | 2 cut(s) | 677, 804 |
| StyD4I | CCNGG | 2 cut(s) | 219, 763 |
| TaiI | ACGT | 1 cut(s) | 763 |
| TaqI | TCGA | 1 cut(s) | 880 |
| TasI | AATT | 4 cut(s) | 75, 460, 707, 888 |
| TatI | WGTACW | 1 cut(s) | 583 |
| TfiI | GAWTC | 4 cut(s) | 32, 158, 200, 305 |
| TseI | GCWGC | 2 cut(s) | 491, 494 |
| TspDTI | ATGAA | 5 cut(s) | 165, 222, 327, 417, 589 |
| TspGWI | ACGGA | 1 cut(s) | 314 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 623 |
| VpaK11BI | GGWCC | 1 cut(s) | 829 |
| XapI | RAATTY | 2 cut(s) | 460, 888 |
| XbaI | TCTAGA | 1 cut(s) | 676 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 445 |
| XhoI | CTCGAG | 1 cut(s) | 879 |
| XmnI | GAANNNNTTC | 1 cut(s) | 596 |
| XspI | CTAG | 2 cut(s) | 677, 804 |
| ZrmI | AGTACT | 1 cut(s) | 585 |