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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 215 |
| Acc16I | TGCGCA | 1 cut(s) | 270 |
| AccII | CGCG | 2 cut(s) | 263, 304 |
| AciI | CCGC | 6 cut(s) | 66, 127, 253, 261, 279, 302 |
| AcoI | YGGCCR | 1 cut(s) | 331 |
| AcsI | RAATTY | 1 cut(s) | 172 |
| AcuI | CTGAAG | 2 cut(s) | 414, 519 |
| AfaI | GTAC | 2 cut(s) | 85, 568 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 126 |
| AjnI | CCWGG | 1 cut(s) | 98 |
| AluBI | AGCT | 1 cut(s) | 47 |
| AluI | AGCT | 1 cut(s) | 47 |
| AoxI | GGCC | 1 cut(s) | 331 |
| ApeKI | GCWGC | 4 cut(s) | 255, 271, 294, 306 |
| ApoI | RAATTY | 1 cut(s) | 172 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 513 |
| AspLEI | GCGC | 2 cut(s) | 271, 306 |
| AspS9I | GGNCC | 1 cut(s) | 96 |
| AvaII | GGWCC | 1 cut(s) | 96 |
| BalI | TGGCCA | 1 cut(s) | 333 |
| BbsI | GAAGAC | 1 cut(s) | 557 |
| BbvI | GCAGC | 4 cut(s) | 242, 283, 293, 306 |
| BccI | CCATC | 1 cut(s) | 148 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 140, 174 |
| BciT130I | CCWGG | 1 cut(s) | 100 |
| BclI | TGATCA | 1 cut(s) | 184 |
| BfmI | CTRYAG | 1 cut(s) | 292 |
| BisI | GCNGC | 7 cut(s) | 128, 253, 256, 272, 279, 295, 307 |
| BlsI | GCNGC | 7 cut(s) | 129, 254, 257, 273, 280, 296, 308 |
| Bme1390I | CCNGG | 1 cut(s) | 100 |
| Bme18I | GGWCC | 1 cut(s) | 96 |
| BmgT120I | GGNCC | 1 cut(s) | 96 |
| BmrFI | CCNGG | 1 cut(s) | 100 |
| BmsI | GCATC | 1 cut(s) | 166 |
| BpiI | GAAGAC | 1 cut(s) | 557 |
| BpuEI | CTTGAG | 1 cut(s) | 189 |
| BsaJI | CCNNGG | 1 cut(s) | 54 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 126 |
| Bse1I | ACTGG | 2 cut(s) | 334, 387 |
| BseBI | CCWGG | 1 cut(s) | 100 |
| BseDI | CCNNGG | 1 cut(s) | 54 |
| BseGI | GGATG | 2 cut(s) | 220, 615 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 126 |
| BseNI | ACTGG | 2 cut(s) | 334, 387 |
| BseXI | GCAGC | 4 cut(s) | 242, 283, 293, 306 |
| BseYI | CCCAGC | 1 cut(s) | 630 |
| BsgI | GTGCAG | 2 cut(s) | 241, 292 |
| Bsh1236I | CGCG | 2 cut(s) | 263, 304 |
| BshFI | GGCC | 1 cut(s) | 333 |
| BsiSI | CCGG | 1 cut(s) | 32 |
| BslFI | GGGAC | 2 cut(s) | 203, 613 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 126 |
| BsmFI | GGGAC | 2 cut(s) | 203, 613 |
| BsnI | GGCC | 1 cut(s) | 333 |
| Bsp1407I | TGTACA | 1 cut(s) | 83 |
| Bsp143I | GATC | 1 cut(s) | 184 |
| BspACI | CCGC | 6 cut(s) | 66, 127, 253, 261, 279, 302 |
| BspANI | GGCC | 1 cut(s) | 333 |
| BspFNI | CGCG | 2 cut(s) | 263, 304 |
| BspMAI | CTGCAG | 1 cut(s) | 296 |
| BsrGI | TGTACA | 1 cut(s) | 83 |
| BsrI | ACTGG | 2 cut(s) | 334, 387 |
| BssECI | CCNNGG | 1 cut(s) | 54 |
| BssMI | GATC | 1 cut(s) | 184 |
| Bst2UI | CCWGG | 1 cut(s) | 100 |
| Bst6I | CTCTTC | 2 cut(s) | 611, 631 |
| BstAUI | TGTACA | 1 cut(s) | 83 |
| BstC8I | GCNNGC | 4 cut(s) | 75, 149, 250, 276 |
| BstDEI | CTNAG | 1 cut(s) | 522 |
| BstF5I | GGATG | 2 cut(s) | 220, 615 |
| BstFNI | CGCG | 2 cut(s) | 263, 304 |
| BstHHI | GCGC | 2 cut(s) | 271, 306 |
| BstKTI | GATC | 1 cut(s) | 187 |
| BstMBI | GATC | 1 cut(s) | 184 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 249, 275, 303 |
| BstNI | CCWGG | 1 cut(s) | 100 |
| BstSCI | CCNGG | 1 cut(s) | 98 |
| BstSFI | CTRYAG | 1 cut(s) | 292 |
| BstUI | CGCG | 2 cut(s) | 263, 304 |
| BstV1I | GCAGC | 4 cut(s) | 242, 283, 293, 306 |
| BstV2I | GAAGAC | 1 cut(s) | 557 |
| BsuRI | GGCC | 1 cut(s) | 333 |
| BtsCI | GGATG | 2 cut(s) | 220, 615 |
| Cac8I | GCNNGC | 4 cut(s) | 75, 149, 250, 276 |
| CfoI | GCGC | 2 cut(s) | 271, 306 |
| Cfr13I | GGNCC | 1 cut(s) | 96 |
| Csp6I | GTAC | 2 cut(s) | 84, 567 |
| CviJI | RGCY | 3 cut(s) | 31, 47, 333 |
| CviKI_1 | RGCY | 3 cut(s) | 31, 47, 333 |
| CviQI | GTAC | 2 cut(s) | 84, 567 |
| DdeI | CTNAG | 1 cut(s) | 522 |
| DpnI | GATC | 1 cut(s) | 186 |
| DpnII | GATC | 1 cut(s) | 184 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 215 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 215 |
| EaeI | YGGCCR | 1 cut(s) | 331 |
| Eam1104I | CTCTTC | 2 cut(s) | 611, 631 |
| EarI | CTCTTC | 2 cut(s) | 611, 631 |
| Eco47I | GGWCC | 1 cut(s) | 96 |
| Eco57I | CTGAAG | 2 cut(s) | 414, 519 |
| EcoRI | GAATTC | 1 cut(s) | 172 |
| EcoRII | CCWGG | 1 cut(s) | 98 |
| FaiI | YATR | 5 cut(s) | 94, 169, 230, 241, 650 |
| FaqI | GGGAC | 2 cut(s) | 203, 613 |
| FauI | CCCGC | 1 cut(s) | 59 |
| FbaI | TGATCA | 1 cut(s) | 184 |
| Fnu4HI | GCNGC | 7 cut(s) | 128, 253, 256, 272, 279, 295, 307 |
| FokI | GGATG | 2 cut(s) | 227, 622 |
| Fsp4HI | GCNGC | 7 cut(s) | 128, 253, 256, 272, 279, 295, 307 |
| FspI | TGCGCA | 1 cut(s) | 270 |
| GlaI | GCGC | 2 cut(s) | 270, 305 |
| GluI | GCNGC | 7 cut(s) | 128, 253, 256, 272, 279, 295, 307 |
| GsaI | CCCAGC | 1 cut(s) | 634 |
| HaeIII | GGCC | 1 cut(s) | 333 |
| HapII | CCGG | 1 cut(s) | 32 |
| HhaI | GCGC | 2 cut(s) | 271, 306 |
| Hin6I | GCGC | 2 cut(s) | 269, 304 |
| HinP1I | GCGC | 2 cut(s) | 269, 304 |
| HinfI | GANTC | 3 cut(s) | 116, 232, 407 |
| HpaII | CCGG | 1 cut(s) | 32 |
| Hpy166II | GTNNAC | 1 cut(s) | 84 |
| Hpy188I | TCNGA | 2 cut(s) | 55, 121 |
| Hpy188III | TCNNGA | 2 cut(s) | 179, 206 |
| Hpy8I | GTNNAC | 1 cut(s) | 84 |
| Hpy99I | CGWCG | 1 cut(s) | 592 |
| HpyAV | CCTTC | 3 cut(s) | 337, 380, 571 |
| HpyCH4V | TGCA | 6 cut(s) | 14, 137, 157, 258, 294, 309 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 249, 275, 303 |
| HpyF3I | CTNAG | 1 cut(s) | 522 |
| HspAI | GCGC | 2 cut(s) | 269, 304 |
| Ksp22I | TGATCA | 1 cut(s) | 184 |
| Kzo9I | GATC | 1 cut(s) | 184 |
| LpnPI | CCDG | 7 cut(s) | 45, 85, 112, 315, 368, 472, 644 |
| Lsp1109I | GCAGC | 4 cut(s) | 242, 283, 293, 306 |
| LweI | GCATC | 1 cut(s) | 166 |
| MaeIII | GTNAC | 1 cut(s) | 325 |
| MalI | GATC | 1 cut(s) | 186 |
| MboI | GATC | 1 cut(s) | 184 |
| MfeI | CAATTG | 1 cut(s) | 152 |
| MlsI | TGGCCA | 1 cut(s) | 333 |
| MluCI | AATT | 3 cut(s) | 87, 152, 172 |
| MluNI | TGGCCA | 1 cut(s) | 333 |
| MlyI | GAGTC | 1 cut(s) | 125 |
| MmeI | TCCRAC | 1 cut(s) | 171 |
| MnlI | CCTC | 4 cut(s) | 49, 61, 585, 612 |
| Mox20I | TGGCCA | 1 cut(s) | 333 |
| MroXI | GAANNNNTTC | 1 cut(s) | 513 |
| MscI | TGGCCA | 1 cut(s) | 333 |
| Msp20I | TGGCCA | 1 cut(s) | 333 |
| MspA1I | CMGCKG | 1 cut(s) | 255 |
| MspI | CCGG | 1 cut(s) | 32 |
| MspR9I | CCNGG | 1 cut(s) | 100 |
| MunI | CAATTG | 1 cut(s) | 152 |
| MvaI | CCWGG | 1 cut(s) | 100 |
| MvnI | CGCG | 2 cut(s) | 263, 304 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 249, 275, 303 |
| NdeII | GATC | 1 cut(s) | 184 |
| NsbI | TGCGCA | 1 cut(s) | 270 |
| PdmI | GAANNNNTTC | 1 cut(s) | 513 |
| PfeI | GAWTC | 2 cut(s) | 232, 407 |
| PkrI | GCNGC | 7 cut(s) | 129, 254, 257, 273, 280, 296, 308 |
| PleI | GAGTC | 1 cut(s) | 124 |
| PpsI | GAGTC | 1 cut(s) | 124 |
| Psp6I | CCWGG | 1 cut(s) | 98 |
| PspFI | CCCAGC | 1 cut(s) | 630 |
| PspGI | CCWGG | 1 cut(s) | 98 |
| PspPI | GGNCC | 1 cut(s) | 96 |
| PstI | CTGCAG | 1 cut(s) | 296 |
| RsaI | GTAC | 2 cut(s) | 85, 568 |
| RsaNI | GTAC | 2 cut(s) | 84, 567 |
| SatI | GCNGC | 7 cut(s) | 128, 253, 256, 272, 279, 295, 307 |
| Sau3AI | GATC | 1 cut(s) | 184 |
| Sau96I | GGNCC | 1 cut(s) | 96 |
| SchI | GAGTC | 1 cut(s) | 125 |
| ScrFI | CCNGG | 1 cut(s) | 100 |
| SetI | ASST | 3 cut(s) | 49, 53, 60 |
| SfaNI | GCATC | 1 cut(s) | 166 |
| SfcI | CTRYAG | 1 cut(s) | 292 |
| SinI | GGWCC | 1 cut(s) | 96 |
| SmlI | CTYRAG | 1 cut(s) | 204 |
| SmoI | CTYRAG | 1 cut(s) | 204 |
| Sse9I | AATT | 3 cut(s) | 87, 152, 172 |
| SsiI | CCGC | 6 cut(s) | 66, 127, 253, 261, 279, 302 |
| StyD4I | CCNGG | 1 cut(s) | 98 |
| TaqI | TCGA | 1 cut(s) | 160 |
| TasI | AATT | 3 cut(s) | 87, 152, 172 |
| TatI | WGTACW | 2 cut(s) | 83, 566 |
| TauI | GCSGC | 3 cut(s) | 130, 255, 281 |
| TfiI | GAWTC | 2 cut(s) | 232, 407 |
| TseI | GCWGC | 4 cut(s) | 255, 271, 294, 306 |
| TspDTI | ATGAA | 5 cut(s) | 245, 414, 429, 519, 629 |
| VpaK11BI | GGWCC | 1 cut(s) | 96 |
| XapI | RAATTY | 1 cut(s) | 172 |
| XmnI | GAANNNNTTC | 1 cut(s) | 513 |