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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1882 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1073 |
| AccII | CGCG | 1 cut(s) | 2075 |
| AciI | CCGC | 3 cut(s) | 80, 941, 1901 |
| AclWI | GGATC | 5 cut(s) | 938, 2464, 2811, 2977, 2990 |
| AcsI | RAATTY | 3 cut(s) | 800, 1851, 2870 |
| AcuI | CTGAAG | 7 cut(s) | 72, 165, 207, 312, 402, 654, 1319 |
| AfaI | GTAC | 5 cut(s) | 469, 864, 2325, 2543, 2585 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 851, 879, 989, 1073, 2069 |
| AjnI | CCWGG | 3 cut(s) | 532, 844, 1726 |
| AjuI | GAANNNNNNNTTGG | 8 cut(s) | 953, 985, 1072, 1104, 1367, 1399, 1563, 1595 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 399, 431 |
| Alw26I | GTCTC | 3 cut(s) | 1005, 1319, 1460 |
| AlwI | GGATC | 5 cut(s) | 938, 2464, 2811, 2977, 2990 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3006 |
| Ama87I | CYCGRG | 1 cut(s) | 1517 |
| AoxI | GGCC | 3 cut(s) | 4, 1791, 2281 |
| ApeKI | GCWGC | 9 cut(s) | 579, 649, 652, 944, 1961, 2437, 2848, 2851, 2859 |
| ApoI | RAATTY | 3 cut(s) | 800, 1851, 2870 |
| AseI | ATTAAT | 1 cut(s) | 1031 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 414, 1046 |
| AspLEI | GCGC | 2 cut(s) | 1895, 2075 |
| AspS9I | GGNCC | 2 cut(s) | 4, 1792 |
| AsuC2I | CCSGG | 1 cut(s) | 2070 |
| AsuHPI | GGTGA | 8 cut(s) | 308, 1294, 1704, 1742, 1926, 1968, 2145, 2224 |
| AvaI | CYCGRG | 1 cut(s) | 1517 |
| AxyI | CCTNAGG | 1 cut(s) | 1797 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2120, 2153 |
| BamHI | GGATCC | 1 cut(s) | 2982 |
| BbsI | GAAGAC | 2 cut(s) | 236, 381 |
| BbvI | GCAGC | 9 cut(s) | 566, 636, 639, 931, 1948, 2449, 2835, 2863, 2871 |
| BccI | CCATC | 4 cut(s) | 398, 1232, 1456, 2902 |
| BceAI | ACGGC | 1 cut(s) | 1010 |
| BciT130I | CCWGG | 3 cut(s) | 534, 846, 1728 |
| BcnI | CCSGG | 1 cut(s) | 2070 |
| BcoDI | GTCTC | 3 cut(s) | 1005, 1319, 1460 |
| BfaI | CTAG | 1 cut(s) | 1767 |
| BfmI | CTRYAG | 3 cut(s) | 653, 945, 2849 |
| BfuAI | ACCTGC | 1 cut(s) | 1882 |
| BglII | AGATCT | 1 cut(s) | 2765 |
| BmcAI | AGTACT | 1 cut(s) | 2585 |
| Bme1390I | CCNGG | 4 cut(s) | 534, 846, 1728, 2070 |
| BmeT110I | CYCGRG | 1 cut(s) | 1517 |
| BmgT120I | GGNCC | 2 cut(s) | 4, 1792 |
| BmiI | GGNNCC | 3 cut(s) | 1794, 1905, 2984 |
| BmrFI | CCNGG | 4 cut(s) | 534, 846, 1728, 2070 |
| BmsI | GCATC | 4 cut(s) | 673, 706, 1168, 1904 |
| BoxI | GACNNNNGTC | 2 cut(s) | 1930, 2880 |
| BpiI | GAAGAC | 2 cut(s) | 236, 381 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1631, 1663 |
| BpmI | CTGGAG | 3 cut(s) | 57, 966, 1095 |
| BpuEI | CTTGAG | 1 cut(s) | 1827 |
| BpuMI | CCSGG | 1 cut(s) | 2070 |
| BsaJI | CCNNGG | 5 cut(s) | 220, 532, 533, 691, 1921 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 399, 429, 1595, 1625, 1738, 1768, 2074, 2104 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 851, 879, 989, 1073, 2069 |
| Bse1I | ACTGG | 5 cut(s) | 262, 285, 1269, 2591, 2702 |
| Bse21I | CCTNAGG | 1 cut(s) | 1797 |
| BseBI | CCWGG | 3 cut(s) | 534, 846, 1728 |
| BseDI | CCNNGG | 5 cut(s) | 220, 532, 533, 691, 1921 |
| BseGI | GGATG | 7 cut(s) | 721, 1723, 2359, 2485, 2556, 2734, 2820 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 851, 879, 989, 1073, 2069 |
| BseMII | CTCAG | 5 cut(s) | 885, 1689, 1811, 2217, 2283 |
| BseNI | ACTGG | 5 cut(s) | 262, 285, 1269, 2591, 2702 |
| BseRI | GAGGAG | 3 cut(s) | 1577, 1740, 1743 |
| BseXI | GCAGC | 9 cut(s) | 566, 636, 639, 931, 1948, 2449, 2835, 2863, 2871 |
| BseYI | CCCAGC | 1 cut(s) | 1140 |
| BsgI | GTGCAG | 3 cut(s) | 565, 590, 1947 |
| Bsh1236I | CGCG | 1 cut(s) | 2075 |
| BshFI | GGCC | 3 cut(s) | 6, 1793, 2283 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1517 |
| BsiSI | CCGG | 1 cut(s) | 2069 |
| BslFI | GGGAC | 3 cut(s) | 515, 652, 1106 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 851, 879, 989, 1073, 2069 |
| BsmAI | GTCTC | 3 cut(s) | 1005, 1319, 1460 |
| BsmFI | GGGAC | 3 cut(s) | 515, 652, 1106 |
| BsmI | GAATGC | 2 cut(s) | 686, 1949 |
| BsnI | GGCC | 3 cut(s) | 6, 1793, 2283 |
| BsoBI | CYCGRG | 1 cut(s) | 1517 |
| Bsp1407I | TGTACA | 1 cut(s) | 2541 |
| Bsp19I | CCATGG | 1 cut(s) | 691 |
| BspACI | CCGC | 3 cut(s) | 80, 941, 1901 |
| BspANI | GGCC | 3 cut(s) | 6, 1793, 2283 |
| BspCNI | CTCAG | 5 cut(s) | 886, 1690, 1810, 2218, 2282 |
| BspFNI | CGCG | 1 cut(s) | 2075 |
| BspLI | GGNNCC | 3 cut(s) | 1794, 1905, 2984 |
| BspMAI | CTGCAG | 3 cut(s) | 657, 949, 2853 |
| BspMI | ACCTGC | 1 cut(s) | 1882 |
| BspPI | GGATC | 5 cut(s) | 938, 2464, 2811, 2977, 2990 |
| BspQI | GCTCTTC | 1 cut(s) | 1527 |
| BsrGI | TGTACA | 1 cut(s) | 2541 |
| BsrI | ACTGG | 5 cut(s) | 262, 285, 1269, 2591, 2702 |
| BssECI | CCNNGG | 5 cut(s) | 220, 532, 533, 691, 1921 |
| BssT1I | CCWWGG | 2 cut(s) | 691, 1921 |
| Bst2UI | CCWGG | 3 cut(s) | 534, 846, 1728 |
| Bst4CI | ACNGT | 3 cut(s) | 1888, 2541, 2975 |
| Bst6I | CTCTTC | 7 cut(s) | 513, 1107, 1485, 1527, 1590, 1990, 2303 |
| BstAUI | TGTACA | 1 cut(s) | 2541 |
| BstC8I | GCNNGC | 3 cut(s) | 591, 1820, 2121 |
| BstDEI | CTNAG | 7 cut(s) | 894, 1355, 1538, 1698, 1797, 2226, 2269 |
| BstDSI | CCRYGG | 1 cut(s) | 691 |
| BstF5I | GGATG | 7 cut(s) | 721, 1723, 2359, 2485, 2556, 2734, 2820 |
| BstFNI | CGCG | 1 cut(s) | 2075 |
| BstHHI | GCGC | 2 cut(s) | 1895, 2075 |
| BstMAI | GTCTC | 3 cut(s) | 1005, 1319, 1460 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 953, 1958, 2125 |
| BstNI | CCWGG | 3 cut(s) | 534, 846, 1728 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 1930, 2880 |
| BstSCI | CCNGG | 4 cut(s) | 532, 844, 1726, 2068 |
| BstSFI | CTRYAG | 3 cut(s) | 653, 945, 2849 |
| BstUI | CGCG | 1 cut(s) | 2075 |
| BstV1I | GCAGC | 9 cut(s) | 566, 636, 639, 931, 1948, 2449, 2835, 2863, 2871 |
| BstV2I | GAAGAC | 2 cut(s) | 236, 381 |
| BstX2I | RGATCY | 2 cut(s) | 2765, 2982 |
| BstYI | RGATCY | 2 cut(s) | 2765, 2982 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1797 |
| BsuRI | GGCC | 3 cut(s) | 6, 1793, 2283 |
| BtgI | CCRYGG | 1 cut(s) | 691 |
| BtgZI | GCGATG | 1 cut(s) | 2943 |
| BtsCI | GGATG | 7 cut(s) | 721, 1723, 2359, 2485, 2556, 2734, 2820 |
| BtsIMutI | CAGTG | 4 cut(s) | 378, 1276, 1649, 2402 |
| BveI | ACCTGC | 1 cut(s) | 1882 |
| Cac8I | GCNNGC | 3 cut(s) | 591, 1820, 2121 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3006 |
| CfoI | GCGC | 2 cut(s) | 1895, 2075 |
| Cfr13I | GGNCC | 2 cut(s) | 4, 1792 |
| Csp6I | GTAC | 5 cut(s) | 468, 863, 2324, 2542, 2584 |
| CviAII | CATG | 8 cut(s) | 692, 1279, 1296, 2236, 2459, 2474, 2785, 2996 |
| CviQI | GTAC | 5 cut(s) | 468, 863, 2324, 2542, 2584 |
| DdeI | CTNAG | 7 cut(s) | 894, 1355, 1538, 1698, 1797, 2226, 2269 |
| Eam1104I | CTCTTC | 7 cut(s) | 513, 1107, 1485, 1527, 1590, 1990, 2303 |
| EarI | CTCTTC | 7 cut(s) | 513, 1107, 1485, 1527, 1590, 1990, 2303 |
| Eco130I | CCWWGG | 2 cut(s) | 691, 1921 |
| Eco57I | CTGAAG | 7 cut(s) | 72, 165, 207, 312, 402, 654, 1319 |
| Eco81I | CCTNAGG | 1 cut(s) | 1797 |
| Eco88I | CYCGRG | 1 cut(s) | 1517 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1792 |
| EcoRI | GAATTC | 1 cut(s) | 1851 |
| EcoRII | CCWGG | 3 cut(s) | 532, 844, 1726 |
| EcoT14I | CCWWGG | 2 cut(s) | 691, 1921 |
| EcoT22I | ATGCAT | 3 cut(s) | 688, 2050, 2781 |
| ErhI | CCWWGG | 2 cut(s) | 691, 1921 |
| FaeI | CATG | 8 cut(s) | 695, 1282, 1299, 2239, 2462, 2477, 2788, 2999 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 2755, 2787, 2971, 3003 |
| FaqI | GGGAC | 3 cut(s) | 515, 652, 1106 |
| FatI | CATG | 8 cut(s) | 691, 1278, 1295, 2235, 2458, 2473, 2784, 2995 |
| FokI | GGATG | 7 cut(s) | 728, 1710, 2366, 2492, 2543, 2741, 2827 |
| FspBI | CTAG | 1 cut(s) | 1767 |
| GlaI | GCGC | 2 cut(s) | 1894, 2074 |
| GsaI | CCCAGC | 1 cut(s) | 1144 |
| GsuI | CTGGAG | 3 cut(s) | 57, 966, 1095 |
| HaeIII | GGCC | 3 cut(s) | 6, 1793, 2283 |
| HapII | CCGG | 1 cut(s) | 2069 |
| HhaI | GCGC | 2 cut(s) | 1895, 2075 |
| Hin1II | CATG | 8 cut(s) | 695, 1282, 1299, 2239, 2462, 2477, 2788, 2999 |
| Hin6I | GCGC | 2 cut(s) | 1893, 2073 |
| HinP1I | GCGC | 2 cut(s) | 1893, 2073 |
| HincII | GTYRAC | 1 cut(s) | 813 |
| HindII | GTYRAC | 1 cut(s) | 813 |
| HindIII | AAGCTT | 1 cut(s) | 954 |
| HpaII | CCGG | 1 cut(s) | 2069 |
| HphI | GGTGA | 8 cut(s) | 308, 1294, 1704, 1742, 1926, 1968, 2145, 2224 |
| Hpy166II | GTNNAC | 4 cut(s) | 325, 813, 2232, 2750 |
| Hpy188III | TCNNGA | 7 cut(s) | 36, 65, 901, 983, 1039, 1074, 1799 |
| Hpy8I | GTNNAC | 4 cut(s) | 325, 813, 2232, 2750 |
| HpyCH4III | ACNGT | 3 cut(s) | 1888, 2541, 2975 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 953, 1958, 2125 |
| HpyF3I | CTNAG | 7 cut(s) | 894, 1355, 1538, 1698, 1797, 2226, 2269 |
| Hsp92II | CATG | 8 cut(s) | 695, 1282, 1299, 2239, 2462, 2477, 2788, 2999 |
| HspAI | GCGC | 2 cut(s) | 1893, 2073 |
| LguI | GCTCTTC | 1 cut(s) | 1527 |
| Lsp1109I | GCAGC | 9 cut(s) | 566, 636, 639, 931, 1948, 2449, 2835, 2863, 2871 |
| LweI | GCATC | 4 cut(s) | 673, 706, 1168, 1904 |
| MaeI | CTAG | 1 cut(s) | 1767 |
| MaeIII | GTNAC | 4 cut(s) | 901, 1710, 1932, 2076 |
| MfeI | CAATTG | 4 cut(s) | 75, 606, 1670, 2191 |
| MflI | RGATCY | 2 cut(s) | 2765, 2982 |
| MlyI | GAGTC | 5 cut(s) | 1718, 1736, 1874, 2261, 2890 |
| Mph1103I | ATGCAT | 3 cut(s) | 688, 2050, 2781 |
| MroXI | GAANNNNTTC | 2 cut(s) | 414, 1046 |
| MseI | TTAA | 9 cut(s) | 89, 705, 1031, 1170, 1449, 1656, 1995, 2043, 2903 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1685, 1728, 1942, 2478 |
| MspA1I | CMGCKG | 1 cut(s) | 2429 |
| MspI | CCGG | 1 cut(s) | 2069 |
| MspR9I | CCNGG | 4 cut(s) | 534, 846, 1728, 2070 |
| MunI | CAATTG | 4 cut(s) | 75, 606, 1670, 2191 |
| Mva1269I | GAATGC | 2 cut(s) | 686, 1949 |
| MvaI | CCWGG | 3 cut(s) | 534, 846, 1728 |
| MvnI | CGCG | 1 cut(s) | 2075 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 953, 1958, 2125 |
| NciI | CCSGG | 1 cut(s) | 2070 |
| NcoI | CCATGG | 1 cut(s) | 691 |
| NlaIII | CATG | 8 cut(s) | 695, 1282, 1299, 2239, 2462, 2477, 2788, 2999 |
| NlaIV | GGNNCC | 3 cut(s) | 1794, 1905, 2984 |
| NmuCI | GTSAC | 3 cut(s) | 901, 1710, 1932 |
| NsiI | ATGCAT | 3 cut(s) | 688, 2050, 2781 |
| PaeR7I | CTCGAG | 1 cut(s) | 1517 |
| PasI | CCCWGGG | 1 cut(s) | 533 |
| PciSI | GCTCTTC | 1 cut(s) | 1527 |
| PctI | GAATGC | 2 cut(s) | 686, 1949 |
| PdmI | GAANNNNTTC | 2 cut(s) | 414, 1046 |
| PfeI | GAWTC | 8 cut(s) | 32, 158, 200, 305, 849, 2273, 2394, 3003 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1073 |
| PleI | GAGTC | 5 cut(s) | 1717, 1736, 1874, 2261, 2889 |
| PpsI | GAGTC | 5 cut(s) | 1717, 1736, 1874, 2261, 2889 |
| PshAI | GACNNNNGTC | 2 cut(s) | 1930, 2880 |
| PshBI | ATTAAT | 1 cut(s) | 1031 |
| Psp6I | CCWGG | 3 cut(s) | 532, 844, 1726 |
| PspFI | CCCAGC | 1 cut(s) | 1140 |
| PspGI | CCWGG | 3 cut(s) | 532, 844, 1726 |
| PspN4I | GGNNCC | 3 cut(s) | 1794, 1905, 2984 |
| PspPI | GGNCC | 2 cut(s) | 4, 1792 |
| PspXI | VCTCGAGB | 1 cut(s) | 1517 |
| PstI | CTGCAG | 3 cut(s) | 657, 949, 2853 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3006 |
| PsuI | RGATCY | 2 cut(s) | 2765, 2982 |
| PvuII | CAGCTG | 1 cut(s) | 2429 |
| RsaI | GTAC | 5 cut(s) | 469, 864, 2325, 2543, 2585 |
| RsaNI | GTAC | 5 cut(s) | 468, 863, 2324, 2542, 2584 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1685, 1728, 1942, 2478 |
| SapI | GCTCTTC | 1 cut(s) | 1527 |
| SaqAI | TTAA | 9 cut(s) | 89, 705, 1031, 1170, 1449, 1656, 1995, 2043, 2903 |
| Sau96I | GGNCC | 2 cut(s) | 4, 1792 |
| ScaI | AGTACT | 1 cut(s) | 2585 |
| SchI | GAGTC | 5 cut(s) | 1718, 1736, 1874, 2261, 2890 |
| ScrFI | CCNGG | 4 cut(s) | 534, 846, 1728, 2070 |
| SfaNI | GCATC | 4 cut(s) | 673, 706, 1168, 1904 |
| SfcI | CTRYAG | 3 cut(s) | 653, 945, 2849 |
| Sfr274I | CTCGAG | 1 cut(s) | 1517 |
| SlaI | CTCGAG | 1 cut(s) | 1517 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1685, 1728, 1942, 2478 |
| SmlI | CTYRAG | 2 cut(s) | 1517, 1842 |
| SmoI | CTYRAG | 2 cut(s) | 1517, 1842 |
| SsiI | CCGC | 3 cut(s) | 80, 941, 1901 |
| SspMI | CTAG | 1 cut(s) | 1767 |
| StyD4I | CCNGG | 4 cut(s) | 532, 844, 1726, 2068 |
| StyI | CCWWGG | 2 cut(s) | 691, 1921 |
| TaaI | ACNGT | 3 cut(s) | 1888, 2541, 2975 |
| TaqI | TCGA | 1 cut(s) | 1518 |
| TatI | WGTACW | 4 cut(s) | 467, 2323, 2541, 2583 |
| TauI | GCSGC | 1 cut(s) | 944 |
| TfiI | GAWTC | 8 cut(s) | 32, 158, 200, 305, 849, 2273, 2394, 3003 |
| Tru1I | TTAA | 9 cut(s) | 89, 705, 1031, 1170, 1449, 1656, 1995, 2043, 2903 |
| Tru9I | TTAA | 9 cut(s) | 89, 705, 1031, 1170, 1449, 1656, 1995, 2043, 2903 |
| TscAI | CASTG | 4 cut(s) | 385, 1276, 1656, 2409 |
| TseFI | GTSAC | 3 cut(s) | 901, 1710, 1932 |
| TseI | GCWGC | 9 cut(s) | 579, 649, 652, 944, 1961, 2437, 2848, 2851, 2859 |
| Tsp45I | GTSAC | 3 cut(s) | 901, 1710, 1932 |
| TspGWI | ACGGA | 2 cut(s) | 2093, 2222 |
| TspRI | CASTG | 4 cut(s) | 385, 1276, 1656, 2409 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1073 |
| VspI | ATTAAT | 1 cut(s) | 1031 |
| XapI | RAATTY | 3 cut(s) | 800, 1851, 2870 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1276 |
| XhoI | CTCGAG | 1 cut(s) | 1517 |
| XmnI | GAANNNNTTC | 2 cut(s) | 414, 1046 |
| XspI | CTAG | 1 cut(s) | 1767 |
| ZrmI | AGTACT | 1 cut(s) | 2585 |
| Zsp2I | ATGCAT | 3 cut(s) | 688, 2050, 2781 |