FvH4_2g27931

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000124
Physical Location & Seq
Forward (+)
22054030 .. 22057181
3152 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g27931.t1

Sequence Viewer

Length: 2070 bp
ATGGAGAAGAAAGAAGTGGGGATGTGGTCTCCAAATCCGAAAGGTGAAAGGACATATGTGGGGGGATGTGTGGCCCCAAGATGTAAGGTAAAGAGTAAAACAAACATTTCGGAGTTCAAATGGATAGTGAGCGAGATTAACGAAGCTAAAAGGGTTGCGGTGAGGGCGGTTGGTTTCGGGTATACATTGGAAATAGGTTGTCATGACTTGGCACAGGCTATAATGGTCGAAACACTGCATCAAGTCGACCACATAGGTTTCAAGATCAGGATACACGATGTTATTTGCACCATAAACGGTGCAGAATTTGGACGTGTAATAGGGATATATGACGGTGATGAAGACGTGGTAATCGGTGACAGCAGTTGTGCTAAAGATTACGGACCAAAGGTGCTTCCGATGTGCGACAAGAACGGGAATATTACGACAGATTTGTTGACTAGCATAGTGAGGGAAAGGAATGAGGCAGACGACACGTTCATCATTGCATATGTCATGTTGGCACTTCATTATGTGATATGCCCAGAAGTAGACCGTAATGTTCAGGTTAGATTGTTGAAGGCAGTAATGGAAACAAACAGAATAGTTAAGAAGAAGTGGGCTTCATTTGCGGTGAGCCGGCTGATGGAGGGTGTAAGGAGGCATAGAGAGGAACAAAAGGAGATTGTGACAGGGTGTATGTTATTTCTGCAGCTTCTATATTTGGACAAGGTGGGGGAGGTTGTTTTACCACCACAGGTGAAAACATGTCCTTTAATGTATTGGACGCCCAAGCTAATAGACGATCTGGTTAACAAAGTTAAAGATGTCGGAGGACACAGCAGTCCAACGGTGTTGTCTTTGAAGAGCAAGAGGACGAAGAAGTTTGAGGAAGCTGGATTTCAGAAGGGCCCAACTGGATTTAAGCTGAAGCAATCTGTCCACGAAGACATTTTGTACGTCCGGGTCAACGTGGACAGAGTCCAAAAGGAGATGTGCGTCATGAACGGGACTGTCAATGACATGTCATCGTACTTTGTGGAAATCAGCAAGGTAGTCTCACAAATAGCAGATGCAGTACACACGATCAAGGGTGAATGTGGTTCCAAAGCTGCCAAGACTTTGGGGTTCAAACGAAACCGACATGATAATGTCAAAGAGGAGGTCGTAGATGTGCAATACAAAACACGAACACAGCAGAGGATGACCTATCCCAAGAATAATGATGTCACTTGTGAAGGAGAGGTTCAATGCATCGGCCATGGGAACATAAGCGGAAAGGTAAACCTTTCTAAGGATGTGGTCACAGGCTACTGGTGGAGTCTAAAATTGGGATGGCGAACCATAGAAACGTGGGGGGGACGTCTACATGTTACGGGGCCTTATAAGCTGTGGGAAAAAATGTCAGAATTGGATGAGAAGATTATCCGCTATGTCTGTATGGTTGGTACGAACCTATCCGAAACTGATAAAAGTGAGTGTGTGGGAATATGTGCCAACAGTATGCAATCAATCACTCGGGCAGAGATAAGGAGTCTGCTGCCAAGCGTTTACATATCAGGAAGTGTAATAAATTACGTGGCTGCCTATCTAAACAATGGTGGAGGCAGGAACTGGTACTGGCCAACACATTTTGAGATATTCATACCAATTCATGATCCTCATATCGACAAAGGACATTGGGTGCTTGTCGTGGTCGACATGGTGAAAAAGGTTGCGGAAGTATGGGACAGCTTATGTGAAAGTGGATTGACTGCACGAAGAATTGACAGAATCTCAAAATTGCTGAAACACATAGACCGGGTTATGCTGTTTGAAAATTATATGTATTGTGATGGCAATAACAGTGTGGGAGATTTTCAAATTTTGGAGGCGGAGAACGTGCCAATTCAGCAGAACACATATGACTGTGGGATATATGTTATACAGAATATGCAACACTATGGGACACACTGGGCATACGAGTATTCGTCAGATCAGCAAAGAGCCGTTATTTTACTGGATTGTGTGAAACCACCAAATAACGAGATGGGTAACGAAATATCCAAACGTAGCAAGGAGTTCACTCATGAGGGGGACAACAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMG_box

Protein Analysis

690

Amino Acids

78.18

Weight (kDa)

7.51

Isoelectric Point (pI)

30.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 540 - 641 1.2e-15 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000124)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10992 FvH4_2g25150 FvH4_2g27910 FvH4_2g27931 FvH4_4g09443 FvH4_4g09490 FvH4_4g15990 FvH4_4g15991 FvH4_6g07180 FvH4_6g40320 FvH4_7g10222 FvH4_7g10251
rosa_chinensis RchiOBHm_Chr2g0155611
rosa_laevigata RLG00000009253 RLG00000009254 RLG00000011848 RLG00000017301 RLG00000017302 RLG00000034909
rosa_multiflora Rmu_co8063658.1_g000001 Rmu_co8240443.1_g000001 Rmu_co8264451.1_g000001 Rmu_co8381937.1_g000001 Rmu_co8419903.1_g000001 Rmu_co8513405.1_g000002 Rmu_co8522915.1_g000001 Rmu_co8522915.1_g000004 Rmu_sc0000279.1_g000024 Rmu_sc0000279.1_g000048 Rmu_sc0000642.1_g000017 Rmu_sc0002045.1_g000015 Rmu_sc0002045.1_g000018 Rmu_sc0002045.1_g000023 Rmu_sc0002045.1_g000028 Rmu_sc0003047.1_g000008 Rmu_sc0003179.1_g000019 Rmu_sc0003179.1_g000020 Rmu_sc0003179.1_g000022 Rmu_sc0003179.1_g000023 Rmu_sc0003422.1_g000011 Rmu_sc0004999.1_g000014 Rmu_sc0005712.1_g000018 Rmu_sc0005712.1_g000023 Rmu_sc0006198.1_g000019 Rmu_sc0006373.1_g000005 Rmu_sc0006514.1_g000028 Rmu_sc0006514.1_g000031 Rmu_sc0007367.1_g000010 Rmu_sc0011475.1_g000002 Rmu_sc0025304.1_g000001 Rmu_ssc0000161.1_g000030 Rmu_ssc0000161.1_g000032 Rmu_ssc0000387.1_g000018
rosa_roxburghii Rroxscaffold_1G00031090 Rroxscaffold_1G00032770 Rroxscaffold_1G00068950 Rroxscaffold_1G00069000 Rroxscaffold_1G00069010 Rroxscaffold_2G00129060 Rroxscaffold_2G00129100 Rroxscaffold_4G00309310 Rroxscaffold_4G00309320 Rroxscaffold_5G00350620 Rroxscaffold_5G00350630 Rroxscaffold_7G00162650 Rroxscaffold_7G00162660 Rroxscaffold_7G00162710
rosa_rugosa Rorug02G0564700 Rorug02G0564700 Rorug03G0343100 Rorug04G0189700 Rorug05G0102800 Rorug05G0102900 Rorug05G0103000 Rorug05G0106500 Rorug05G0106600 Rorug05G0106700 Rorug05G0252800 Rorug07G0182100 Rorug07G0283500 Rorug07G0283600
rosa_samantha Rh1AG004000 Rh1AG004100 Rh1AG004200 Rh1AG004400 Rh1AG004500 Rh1AG004600 Rh1AG004800 Rh1AG004900 Rh1AG005300 Rh1AG007000 Rh1AG115000 Rh1CG049400 Rh1CG055400 Rh1CG111000 Rh1DG053600 Rh1DG053700 Rh1DG059300 Rh1DG119700 Rh1DG177100 Rh1DG177200 Rh2AG353800 Rh2AG354000 Rh2AG354100 Rh2AG354300 Rh2AG354400 Rh2AG354500 Rh2AG354600 Rh2AG354700 Rh2AG642300 Rh2AG642400 Rh2AG642500 Rh2BG336500 Rh2BG336600 Rh2BG336700 Rh2BG336800 Rh2BG336900 Rh2CG299900 Rh2CG300000 Rh2CG300100 Rh2DG337000 Rh2DG337100 Rh3AG246600 Rh3BG121900 Rh3BG122300 Rh3BG122600 Rh3BG122700 Rh3DG304800 Rh3DG304900 Rh4AG148800 Rh4AG249000 Rh4AG249100 Rh4AG249200 Rh4BG182700 Rh4BG182800 Rh4BG182900 Rh4BG254400 Rh4BG254500 Rh4BG254600 Rh4CG155500 Rh4CG155700 Rh4DG057900 Rh4DG058000 Rh4DG058100 Rh4DG058200 Rh4DG129100 Rh5AG364500 Rh5AG364600 Rh5AG364800 Rh5AG365300 Rh5CG363700 Rh5CG497600 Rh5DG239500 Rh5DG239600 Rh6AG173500 Rh6AG175400 Rh6BG075900 Rh6BG076300 Rh6BG076400 Rh6BG076500 Rh6BG076600 Rh6CG151000 Rh6CG151200 Rh6CG151300 Rh6CG151400 Rh6CG205200 Rh6CG205300 Rh6CG419700 Rh6CG419900 Rh6CG420100 Rh6DG498700 Rh6DG498800 Rh7AG329000 Rh7AG329100 Rh7AG329300 Rh7AG329400 Rh7AG353000 Rh7AG353100 Rh7AG353300 Rh7AG353400 Rh7AG353500 Rh7CG346400 Rh7CG346500 Rh7CG346700 Rh7CG370800 Rh7CG370900 Rh7CG371000 Rh7CG371100 Rh7DG325000 Rh7DG325100 Rh7DG325200 Rh7DG325300 Rh7DG353100 Rh7DG353200 Rh7DG353300 Rh7DG363500 Rh7DG364000
rosa_wichuraiana Rw0G002290 Rw0G012780 Rw1G000260 Rw1G000270 Rw1G010400 Rw1G010410 Rw1G010420 Rw1G010430 Rw1G010440 Rw2G003210 Rw2G003220 Rw4G010970 Rw5G048520 Rw6G013320 Rw6G013470 Rw6G014940 Rw6G016570 Rw6G016590 Rw6G016600 Rw6G016610 Rw6G016620 Rw6G035450 Rw7G027760 Rw7G030460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1365
AasI GACNNNNNNGTC 1 cut(s) 822
AatII GACGTC 1 cut(s) 1345
AccI GTMKAC 5 cut(s) 182, 246, 531, 1345, 1677
AciI CCGC 7 cut(s) 158, 167, 611, 1254, 1408, 1697, 1853
AclWI GGATC 1 cut(s) 1631
AcoI YGGCCR 2 cut(s) 1237, 1601
AcsI RAATTY 2 cut(s) 305, 1842
AcuI CTGAAG 1 cut(s) 929
AcyI GRCGYC 2 cut(s) 767, 1342
AfaI GTAC 5 cut(s) 938, 1013, 1059, 1429, 1598
AfiI CCNNNNNNNGG 2 cut(s) 625, 1273
AflIII ACRYGT 5 cut(s) 313, 474, 746, 1002, 1348
AgsI TTSAA 8 cut(s) 118, 262, 559, 844, 1111, 1229, 1796, 1841
AjiI CACGTC 2 cut(s) 314, 346
AluBI AGCT 8 cut(s) 146, 694, 775, 875, 907, 1091, 1369, 1713
AluI AGCT 8 cut(s) 146, 694, 775, 875, 907, 1091, 1369, 1713
Alw26I GTCTC 2 cut(s) 33, 1042
AlwI GGATC 1 cut(s) 1631
AlwNI CAGNNNCTG 1 cut(s) 1593
Ama87I CYCGRG 1 cut(s) 1497
AoxI GGCC 5 cut(s) 72, 889, 1237, 1358, 1601
ApaI GGGCCC 1 cut(s) 893
ApeKI GCWGC 4 cut(s) 691, 1091, 1519, 1562
ApoI RAATTY 2 cut(s) 305, 1842
ArsI GACNNNNNNTTYG 2 cut(s) 1128, 1160
AspS9I GGNCC 5 cut(s) 73, 383, 889, 890, 1358
AsuC2I CCSGG 2 cut(s) 944, 1781
AsuHPI GGTGA 8 cut(s) 56, 172, 347, 368, 625, 751, 1085, 1696
AvaI CYCGRG 1 cut(s) 1497
AvaII GGWCC 1 cut(s) 383
BaeGI GKGCMC 1 cut(s) 893
BalI TGGCCA 1 cut(s) 1603
BanII GRGCYC 1 cut(s) 893
BbsI GAAGAC 2 cut(s) 348, 933
BbvI GCAGC 4 cut(s) 703, 1078, 1506, 1549
BccI CCATC 4 cut(s) 619, 1308, 1808, 2002
BceAI ACGGC 1 cut(s) 1952
BciVI GTATCC 1 cut(s) 264
BcnI CCSGG 2 cut(s) 944, 1781
BcoDI GTCTC 2 cut(s) 33, 1042
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 689
BfuI GTATCC 1 cut(s) 264
BisI GCNGC 4 cut(s) 692, 1092, 1520, 1563
BlsI GCNGC 4 cut(s) 693, 1093, 1521, 1564
Bme1390I CCNGG 2 cut(s) 944, 1781
Bme18I GGWCC 1 cut(s) 383
BmeT110I CYCGRG 1 cut(s) 1497
BmgBI CACGTC 2 cut(s) 314, 346
BmgT120I GGNCC 5 cut(s) 73, 383, 889, 890, 1358
BmiI GGNNCC 4 cut(s) 75, 891, 1084, 1359
BmrFI CCNGG 2 cut(s) 944, 1781
BmrI ACTGGG 1 cut(s) 1942
BmsI GCATC 3 cut(s) 247, 1042, 1242
BmuI ACTGGG 1 cut(s) 1942
BpiI GAAGAC 2 cut(s) 348, 933
BpuMI CCSGG 2 cut(s) 944, 1781
BsaAI YACGTR 1 cut(s) 1558
BsaHI GRCGYC 2 cut(s) 767, 1342
BsaI GGTCTC 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 1240
Bsc4I CCNNNNNNNGG 2 cut(s) 625, 1273
Bse118I RCCGGY 1 cut(s) 618
Bse1I ACTGG 6 cut(s) 901, 1298, 1598, 1604, 1937, 1983
Bse3DI GCAATG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 1240
BseGI GGATG 6 cut(s) 27, 71, 1188, 1282, 1319, 1399
BseLI CCNNNNNNNGG 2 cut(s) 625, 1273
BseMI GCAATG 1 cut(s) 483
BseNI ACTGG 6 cut(s) 901, 1298, 1598, 1604, 1937, 1983
BseRI GAGGAG 1 cut(s) 1154
BseSI GKGCMC 1 cut(s) 893
BseXI GCAGC 4 cut(s) 703, 1078, 1506, 1549
BsgI GTGCAG 2 cut(s) 321, 1719
BshFI GGCC 5 cut(s) 74, 891, 1239, 1360, 1603
BsiHKCI CYCGRG 1 cut(s) 1497
BsiSI CCGG 3 cut(s) 619, 943, 1780
BslFI GGGAC 4 cut(s) 1003, 1353, 1721, 1939
BslI CCNNNNNNNGG 2 cut(s) 625, 1273
BsmAI GTCTC 2 cut(s) 33, 1042
BsmFI GGGAC 4 cut(s) 1003, 1353, 1721, 1939
BsnI GGCC 5 cut(s) 74, 891, 1239, 1360, 1603
Bso31I GGTCTC 1 cut(s) 33
BsoBI CYCGRG 1 cut(s) 1497
Bsp120I GGGCCC 1 cut(s) 889
Bsp1286I GDGCHC 1 cut(s) 893
Bsp143I GATC 5 cut(s) 264, 784, 1065, 1636, 1954
Bsp19I CCATGG 1 cut(s) 1240
BspACI CCGC 7 cut(s) 158, 167, 611, 1254, 1408, 1697, 1853
BspANI GGCC 5 cut(s) 74, 891, 1239, 1360, 1603
BspHI TCATGA 4 cut(s) 202, 981, 1633, 2047
BspLI GGNNCC 4 cut(s) 75, 891, 1084, 1359
BspMAI CTGCAG 1 cut(s) 693
BspPI GGATC 1 cut(s) 1631
BspQI GCTCTTC 1 cut(s) 839
BspTNI GGTCTC 1 cut(s) 33
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 618
BsrI ACTGG 6 cut(s) 901, 1298, 1598, 1604, 1937, 1983
BssAI RCCGGY 1 cut(s) 618
BssECI CCNNGG 1 cut(s) 1240
BssMI GATC 5 cut(s) 264, 784, 1065, 1636, 1954
BssNAI GTATAC 1 cut(s) 183
BssNI GRCGYC 2 cut(s) 767, 1342
BssT1I CCWWGG 1 cut(s) 1240
Bst1107I GTATAC 1 cut(s) 183
Bst4CI ACNGT 8 cut(s) 299, 335, 536, 832, 994, 1481, 1826, 1889
Bst6I CTCTTC 1 cut(s) 839
BstACI GRCGYC 2 cut(s) 767, 1342
BstBAI YACGTR 1 cut(s) 1558
BstC8I GCNNGC 1 cut(s) 620
BstDEI CTNAG 1 cut(s) 1272
BstDSI CCRYGG 1 cut(s) 1240
BstENI CCTNNNNNAGG 1 cut(s) 1271
BstF5I GGATG 6 cut(s) 27, 71, 1188, 1282, 1319, 1399
BstKTI GATC 5 cut(s) 267, 787, 1068, 1639, 1957
BstMAI GTCTC 2 cut(s) 33, 1042
BstMBI GATC 5 cut(s) 264, 784, 1065, 1636, 1954
BstMWI GCNNNNNNNGC 4 cut(s) 164, 608, 1366, 1870
BstNSI RCATGY 3 cut(s) 750, 1006, 1352
BstSCI CCNGG 2 cut(s) 942, 1779
BstSFI CTRYAG 1 cut(s) 689
BstSLI GKGCMC 1 cut(s) 893
BstV1I GCAGC 4 cut(s) 703, 1078, 1506, 1549
BstV2I GAAGAC 2 cut(s) 348, 933
BstXI CCANNNNNNTGG 1 cut(s) 1102
BstZ17I GTATAC 1 cut(s) 183
BsuI GTATCC 1 cut(s) 264
BsuRI GGCC 5 cut(s) 74, 891, 1239, 1360, 1603
BtgI CCRYGG 1 cut(s) 1240
BtrI CACGTC 2 cut(s) 314, 346
BtsCI GGATG 6 cut(s) 27, 71, 1188, 1282, 1319, 1399
BtsI GCAGTG 1 cut(s) 233
BtsIMutI CAGTG 3 cut(s) 233, 1831, 1930
Cac8I GCNNGC 1 cut(s) 620
CaiI CAGNNNCTG 1 cut(s) 1593
CciI TCATGA 4 cut(s) 202, 981, 1633, 2047
Cfr10I RCCGGY 1 cut(s) 618
Cfr13I GGNCC 5 cut(s) 73, 383, 889, 890, 1358
CseI GACGC 2 cut(s) 775, 967
Csp6I GTAC 5 cut(s) 937, 1012, 1058, 1428, 1597
CviQI GTAC 5 cut(s) 937, 1012, 1058, 1428, 1597
DdeI CTNAG 1 cut(s) 1272
DpnI GATC 5 cut(s) 266, 786, 1067, 1638, 1956
DpnII GATC 5 cut(s) 264, 784, 1065, 1636, 1954
DrdI GACNNNNNNGTC 1 cut(s) 822
DseDI GACNNNNNNGTC 1 cut(s) 822
EaeI YGGCCR 2 cut(s) 1237, 1601
Eam1104I CTCTTC 1 cut(s) 839
EarI CTCTTC 1 cut(s) 839
EciI GGCGGA 1 cut(s) 1868
Eco130I CCWWGG 1 cut(s) 1240
Eco24I GRGCYC 1 cut(s) 893
Eco31I GGTCTC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 383
Eco57I CTGAAG 1 cut(s) 929
Eco88I CYCGRG 1 cut(s) 1497
EcoNI CCTNNNNNAGG 1 cut(s) 1271
EcoO109I RGGNCCY 2 cut(s) 889, 1358
EcoT14I CCWWGG 1 cut(s) 1240
EcoT22I ATGCAT 1 cut(s) 1235
EcoT38I GRGCYC 1 cut(s) 893
ErhI CCWWGG 1 cut(s) 1240
FaqI GGGAC 4 cut(s) 1003, 1353, 1721, 1939
FauNDI CATATG 3 cut(s) 55, 490, 1882
FblI GTMKAC 5 cut(s) 182, 246, 531, 1345, 1677
Fnu4HI GCNGC 4 cut(s) 692, 1092, 1520, 1563
FokI GGATG 6 cut(s) 34, 78, 1195, 1289, 1326, 1406
FriOI GRGCYC 1 cut(s) 893
Fsp4HI GCNGC 4 cut(s) 692, 1092, 1520, 1563
FspBI CTAG 1 cut(s) 441
GluI GCNGC 4 cut(s) 692, 1092, 1520, 1563
HaeIII GGCC 5 cut(s) 74, 891, 1239, 1360, 1603
HapII CCGG 3 cut(s) 619, 943, 1780
HgaI GACGC 2 cut(s) 775, 967
Hin1I GRCGYC 2 cut(s) 767, 1342
HincII GTYRAC 5 cut(s) 247, 438, 793, 949, 1678
HindII GTYRAC 5 cut(s) 247, 438, 793, 949, 1678
HinfI GANTC 4 cut(s) 960, 1300, 1513, 1752
HpaI GTTAAC 1 cut(s) 793
HpaII CCGG 3 cut(s) 619, 943, 1780
HphI GGTGA 8 cut(s) 56, 172, 347, 368, 625, 751, 1085, 1696
Hpy188I TCNGA 8 cut(s) 39, 112, 399, 812, 885, 1387, 1441, 1954
Hpy188III TCNNGA 7 cut(s) 203, 262, 268, 982, 1539, 1634, 2048
HpyAV CCTTC 3 cut(s) 553, 880, 1211
HpyCH4III ACNGT 8 cut(s) 299, 335, 536, 832, 994, 1481, 1826, 1889
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 608, 1366, 1870
HpyF3I CTNAG 1 cut(s) 1272
Hsp92I GRCGYC 2 cut(s) 767, 1342
KroI GCCGGC 1 cut(s) 618
KroNI GCCGGC 1 cut(s) 620
KspAI GTTAAC 1 cut(s) 793
Kzo9I GATC 5 cut(s) 264, 784, 1065, 1636, 1954
LguI GCTCTTC 1 cut(s) 839
Lsp1109I GCAGC 4 cut(s) 703, 1078, 1506, 1549
LweI GCATC 3 cut(s) 247, 1042, 1242
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 6 cut(s) 356, 667, 1207, 1282, 1351, 2012
MalI GATC 5 cut(s) 266, 786, 1067, 1638, 1956
MboI GATC 5 cut(s) 264, 784, 1065, 1636, 1954
MboII GAAGA 8 cut(s) 19, 353, 604, 856, 871, 938, 1411, 1752
MhlI GDGCHC 1 cut(s) 893
MlsI TGGCCA 1 cut(s) 1603
MluNI TGGCCA 1 cut(s) 1603
MlyI GAGTC 3 cut(s) 969, 1309, 1522
MmeI TCCRAC 2 cut(s) 790, 851
Mox20I TGGCCA 1 cut(s) 1603
Mph1103I ATGCAT 1 cut(s) 1235
MroNI GCCGGC 1 cut(s) 618
MscI TGGCCA 1 cut(s) 1603
MseI TTAA 6 cut(s) 138, 588, 755, 792, 801, 903
MslI CAYNNNNRTG 1 cut(s) 1128
Msp20I TGGCCA 1 cut(s) 1603
MspI CCGG 3 cut(s) 619, 943, 1780
MspR9I CCNGG 2 cut(s) 944, 1781
MwoI GCNNNNNNNGC 4 cut(s) 164, 608, 1366, 1870
NaeI GCCGGC 1 cut(s) 620
NciI CCSGG 2 cut(s) 944, 1781
NcoI CCATGG 1 cut(s) 1240
NdeI CATATG 3 cut(s) 55, 490, 1882
NdeII GATC 5 cut(s) 264, 784, 1065, 1636, 1954
NgoMIV GCCGGC 1 cut(s) 618
NlaIV GGNNCC 4 cut(s) 75, 891, 1084, 1359
NmuCI GTSAC 4 cut(s) 356, 667, 1207, 1282
NsiI ATGCAT 1 cut(s) 1235
NspI RCATGY 3 cut(s) 750, 1006, 1352
PagI TCATGA 4 cut(s) 202, 981, 1633, 2047
PciI ACATGT 3 cut(s) 746, 1002, 1348
PciSI GCTCTTC 1 cut(s) 839
PdiI GCCGGC 1 cut(s) 620
PfeI GAWTC 1 cut(s) 1752
PflFI GACNNNGTC 1 cut(s) 959
PkrI GCNGC 4 cut(s) 693, 1093, 1521, 1564
PleI GAGTC 3 cut(s) 968, 1308, 1521
PpsI GAGTC 3 cut(s) 968, 1308, 1521
Ppu21I YACGTR 1 cut(s) 1558
PscI ACATGT 3 cut(s) 746, 1002, 1348
PsiI TTATAA 1 cut(s) 1365
PspN4I GGNNCC 4 cut(s) 75, 891, 1084, 1359
PspOMI GGGCCC 1 cut(s) 889
PspPI GGNCC 5 cut(s) 73, 383, 889, 890, 1358
PstI CTGCAG 1 cut(s) 693
PstNI CAGNNNCTG 1 cut(s) 1593
PsyI GACNNNGTC 1 cut(s) 959
RsaI GTAC 5 cut(s) 938, 1013, 1059, 1429, 1598
RsaNI GTAC 5 cut(s) 937, 1012, 1058, 1428, 1597
RseI CAYNNNNRTG 1 cut(s) 1128
SalI GTCGAC 2 cut(s) 245, 1676
SapI GCTCTTC 1 cut(s) 839
SaqAI TTAA 6 cut(s) 138, 588, 755, 792, 801, 903
SatI GCNGC 4 cut(s) 692, 1092, 1520, 1563
Sau3AI GATC 5 cut(s) 264, 784, 1065, 1636, 1954
Sau96I GGNCC 5 cut(s) 73, 383, 889, 890, 1358
SchI GAGTC 3 cut(s) 969, 1309, 1522
ScrFI CCNGG 2 cut(s) 944, 1781
SduI GDGCHC 1 cut(s) 893
SfaNI GCATC 3 cut(s) 247, 1042, 1242
SfcI CTRYAG 1 cut(s) 689
SinI GGWCC 1 cut(s) 383
SmiMI CAYNNNNRTG 1 cut(s) 1128
SsiI CCGC 7 cut(s) 158, 167, 611, 1254, 1408, 1697, 1853
SspI AATATT 1 cut(s) 421
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 2 cut(s) 942, 1779
StyI CCWWGG 1 cut(s) 1240
TaaI ACNGT 8 cut(s) 299, 335, 536, 832, 994, 1481, 1826, 1889
TaqI TCGA 4 cut(s) 228, 246, 1647, 1677
TatI WGTACW 1 cut(s) 1057
TfiI GAWTC 1 cut(s) 1752
Tru1I TTAA 6 cut(s) 138, 588, 755, 792, 801, 903
Tru9I TTAA 6 cut(s) 138, 588, 755, 792, 801, 903
TscAI CASTG 3 cut(s) 240, 1831, 1937
TseFI GTSAC 4 cut(s) 356, 667, 1207, 1282
TseI GCWGC 4 cut(s) 691, 1091, 1519, 1562
Tsp45I GTSAC 4 cut(s) 356, 667, 1207, 1282
TspDTI ATGAA 7 cut(s) 354, 469, 497, 594, 998, 1612, 1622
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 3 cut(s) 240, 1831, 1937
Tth111I GACNNNGTC 1 cut(s) 959
VpaK11BI GGWCC 1 cut(s) 383
XagI CCTNNNNNAGG 1 cut(s) 1271
XapI RAATTY 2 cut(s) 305, 1842
XceI RCATGY 3 cut(s) 750, 1006, 1352
XmiI GTMKAC 5 cut(s) 182, 246, 531, 1345, 1677
XspI CTAG 1 cut(s) 441
ZraI GACGTC 1 cut(s) 1343
Zsp2I ATGCAT 1 cut(s) 1235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.