Rh5CG497600

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000124
Physical Location & Seq
Reverse (-)
69217573 .. 69221116
3544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG497600.1

Sequence Viewer

Length: 2127 bp
ATGGGAGTGCAGGATGGAGGCTCTGACTTTGAACTGGGGACTGCCACAGACGTTGCTGAGTATGGCATGATTGTTGAAGACATGTGTGACAGAGAAGGAAGGATCCAGTACTGTCATCTGAAGGAAATAGTACGGGACCGTGAGGAAGCCGACGACAAGTTCAGGATCGCATTTTCGTTGCTTGCACTAGGTTTGGTTCTGTGCCCTACAACAGATGACGCTGTTGACACGTCTCTGATTCTACCCCTTCTGGAAACGGGACGCATTGGGCAGAAGAACTGGGCTTCCTTTTGTCTCCAAAGATTGCTAGACTCTGTGACGGCCCAGAGGGAACATGGGGACGGTGTTGTTGGGGGGTGTGTGCTCTTCCTCCAGGTTCTATACTTCGAGAAGGTCGGCTCGGCTTGTTTTCATATTGATAGGTCTATACTTCCACTTATGGCCTGGGGGGACTTTGAGGTAAGGGAGTTGATAACCAGGGTTAGGACATATGGTGGCTTTAAGAGTCCAATTGTCATGATGGAGAGGAACAAGCGGGTGAAAATGGCTAGTAAATGCGCCGATGATGATGGTGAGTTTGGACTCGGACTGAGGAAGTCAGTTCATGAGGACATCTTCGACGTCAAGTTGGAGCTCATGGGGGTCAAGGGTGACGTGTGGAGGCTCATGGATGCTTGTGGGAGGATTCAGCCTGATGTGGCCGAACTAGCAAGGAAAGTGGAGATTATAGCACATCAGATAAGCGGTTTGAGGGAGGAATGTGCCGACATGGTGAAGATCCAACTGCTGAGATCGGCAAACAGGATGGGGGATGACTGCAACGTTCACACAGTCCCTGTGTGGAAACCGCGGCAGAGGGATGGAATGGGGAGTGGAGATTCAGTCCTCCATGCTGACACATTTCTGGAGGAAGAGAGCTTGCAGCGAATGAAGGCAAAATCAAAGCTATGTGATAATGCTTTTGGTAAGGCAGTATCTGCCAGCTCAAGCAGTCCTGGTGCCGCCCCAACAAGAGAGATGAGACCATATCAGGGCGGTTGTGTGCCTGAATCCAAGGCAGTTGTATCTCCACATGCCAGGGCCATGTTCACTTATGCAGTCACCCGCAACGAGGAGGTCAATCATGTGATTAGTTCGGGCTGTAATGGAGCAGGGCTCACTAATGCCAAAAGTTGGATGCTGCCAGGACCTTACATGCTGATGCACCCTATAGGGAATGACGACATTCTCCTAATCAATTACATTTTCATGGGTGGGAAGAAAGTTACGGTGGAGGAGGGAAGAACTATGGTTGCTTCAACCGGGAATCAGAAGGCACTACTTTACCGTGATGAGGCCTTATGCTTGGGGCCAGCCACAAATGTTTCCAGCTTTGAAAAGGCCAGTTCGTACAATGCAGGGGAATCGTTTCGGGAATGGGTGGAGCACACAAGGACACTCTGCAACCTTGATAGATTCAACGGTTGCATGAAGAATTGCAAGAGGATGTTTTTGCCGATGCACGAGGACAAGGCCGGGGGACATTGGTTTCTGATGGTGGTTGATCTAGAAAAGAAGGTGGCTGAGGTTTGGGACAGTCTGCCGACTCTGTCATCAGCAAGCAGTAGATGGGACCATGCATTGACAACGCTGCGCCATCTTGACCATTGCTTTTACTTTGACAAGTTGAGGATGGAAGGGACCAACACTGACTTTGGGTTATTCAACTATGTTGAGCCTGTAGATGCACCTAAACAGGCAAATGGTTTCGACTGTGGGGTTTATATCATCCTTAATATGATGCACTACCGGACCAATTGGCTGAAAGGGGCCGGGTCAGATGATCTTAGAGCAAGGGTGCTTCTTCGGTGTCTCAAAGCCCCTGATAATGAGAGGGCTACGGTTACACTAAGAGAAGCACACTCCTTTGCTGTTGATAAGGGATCTGAGGGAGGTATGAAGGAGGACAAGATAGTGGAGCCAAATGGATGGGAAGGTATTGATCTGTCTAAGTTTGATGAACTGGATGATGATTTGGGAGTTACTCAAGTTGGTGATCCGAGTACAGCGCCAATTGGTGAGTCAGGCATATTGAAGAGGAACATGGACCTTAGGGAAGGCTTTCCTAAGGGAATGTTCCGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

708

Amino Acids

78.82

Weight (kDa)

5.52

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 471 - 595 5.2e-13 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000124)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10992 FvH4_2g25150 FvH4_2g27910 FvH4_2g27931 FvH4_4g09443 FvH4_4g09490 FvH4_4g15990 FvH4_4g15991 FvH4_6g07180 FvH4_6g40320 FvH4_7g10222 FvH4_7g10251
rosa_chinensis RchiOBHm_Chr2g0155611
rosa_laevigata RLG00000009253 RLG00000009254 RLG00000011848 RLG00000017301 RLG00000017302 RLG00000034909
rosa_multiflora Rmu_co8063658.1_g000001 Rmu_co8240443.1_g000001 Rmu_co8264451.1_g000001 Rmu_co8381937.1_g000001 Rmu_co8419903.1_g000001 Rmu_co8513405.1_g000002 Rmu_co8522915.1_g000001 Rmu_co8522915.1_g000004 Rmu_sc0000279.1_g000024 Rmu_sc0000279.1_g000048 Rmu_sc0000642.1_g000017 Rmu_sc0002045.1_g000015 Rmu_sc0002045.1_g000018 Rmu_sc0002045.1_g000023 Rmu_sc0002045.1_g000028 Rmu_sc0003047.1_g000008 Rmu_sc0003179.1_g000019 Rmu_sc0003179.1_g000020 Rmu_sc0003179.1_g000022 Rmu_sc0003179.1_g000023 Rmu_sc0003422.1_g000011 Rmu_sc0004999.1_g000014 Rmu_sc0005712.1_g000018 Rmu_sc0005712.1_g000023 Rmu_sc0006198.1_g000019 Rmu_sc0006373.1_g000005 Rmu_sc0006514.1_g000028 Rmu_sc0006514.1_g000031 Rmu_sc0007367.1_g000010 Rmu_sc0011475.1_g000002 Rmu_sc0025304.1_g000001 Rmu_ssc0000161.1_g000030 Rmu_ssc0000161.1_g000032 Rmu_ssc0000387.1_g000018
rosa_roxburghii Rroxscaffold_1G00031090 Rroxscaffold_1G00032770 Rroxscaffold_1G00068950 Rroxscaffold_1G00069000 Rroxscaffold_1G00069010 Rroxscaffold_2G00129060 Rroxscaffold_2G00129100 Rroxscaffold_4G00309310 Rroxscaffold_4G00309320 Rroxscaffold_5G00350620 Rroxscaffold_5G00350630 Rroxscaffold_7G00162650 Rroxscaffold_7G00162660 Rroxscaffold_7G00162710
rosa_rugosa Rorug02G0564700 Rorug02G0564700 Rorug03G0343100 Rorug04G0189700 Rorug05G0102800 Rorug05G0102900 Rorug05G0103000 Rorug05G0106500 Rorug05G0106600 Rorug05G0106700 Rorug05G0252800 Rorug07G0182100 Rorug07G0283500 Rorug07G0283600
rosa_samantha Rh1AG004000 Rh1AG004100 Rh1AG004200 Rh1AG004400 Rh1AG004500 Rh1AG004600 Rh1AG004800 Rh1AG004900 Rh1AG005300 Rh1AG007000 Rh1AG115000 Rh1CG049400 Rh1CG055400 Rh1CG111000 Rh1DG053600 Rh1DG053700 Rh1DG059300 Rh1DG119700 Rh1DG177100 Rh1DG177200 Rh2AG353800 Rh2AG354000 Rh2AG354100 Rh2AG354300 Rh2AG354400 Rh2AG354500 Rh2AG354600 Rh2AG354700 Rh2AG642300 Rh2AG642400 Rh2AG642500 Rh2BG336500 Rh2BG336600 Rh2BG336700 Rh2BG336800 Rh2BG336900 Rh2CG299900 Rh2CG300000 Rh2CG300100 Rh2DG337000 Rh2DG337100 Rh3AG246600 Rh3BG121900 Rh3BG122300 Rh3BG122600 Rh3BG122700 Rh3DG304800 Rh3DG304900 Rh4AG148800 Rh4AG249000 Rh4AG249100 Rh4AG249200 Rh4BG182700 Rh4BG182800 Rh4BG182900 Rh4BG254400 Rh4BG254500 Rh4BG254600 Rh4CG155500 Rh4CG155700 Rh4DG057900 Rh4DG058000 Rh4DG058100 Rh4DG058200 Rh4DG129100 Rh5AG364500 Rh5AG364600 Rh5AG364800 Rh5AG365300 Rh5CG363700 Rh5CG497600 Rh5DG239500 Rh5DG239600 Rh6AG173500 Rh6AG175400 Rh6BG075900 Rh6BG076300 Rh6BG076400 Rh6BG076500 Rh6BG076600 Rh6CG151000 Rh6CG151200 Rh6CG151300 Rh6CG151400 Rh6CG205200 Rh6CG205300 Rh6CG419700 Rh6CG419900 Rh6CG420100 Rh6DG498700 Rh6DG498800 Rh7AG329000 Rh7AG329100 Rh7AG329300 Rh7AG329400 Rh7AG353000 Rh7AG353100 Rh7AG353300 Rh7AG353400 Rh7AG353500 Rh7CG346400 Rh7CG346500 Rh7CG346700 Rh7CG370800 Rh7CG370900 Rh7CG371000 Rh7CG371100 Rh7DG325000 Rh7DG325100 Rh7DG325200 Rh7DG325300 Rh7DG353100 Rh7DG353200 Rh7DG353300 Rh7DG363500 Rh7DG364000
rosa_wichuraiana Rw0G002290 Rw0G012780 Rw1G000260 Rw1G000270 Rw1G010400 Rw1G010410 Rw1G010420 Rw1G010430 Rw1G010440 Rw2G003210 Rw2G003220 Rw4G010970 Rw5G048520 Rw6G013320 Rw6G013470 Rw6G014940 Rw6G016570 Rw6G016590 Rw6G016600 Rw6G016610 Rw6G016620 Rw6G035450 Rw7G027760 Rw7G030460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 624
AccB1I GGYRCC 1 cut(s) 998
AccB7I CCANNNNNTGG 1 cut(s) 1173
AccII CGCG 1 cut(s) 850
AciI CCGC 7 cut(s) 535, 744, 848, 850, 1002, 1035, 1105
AclI AACGTT 1 cut(s) 822
AclWI GGATC 6 cut(s) 97, 110, 173, 772, 1930, 2030
AcoI YGGCCR 1 cut(s) 699
AcuI CTGAAG 1 cut(s) 140
AcyI GRCGYC 1 cut(s) 621
AfaI GTAC 4 cut(s) 110, 132, 1391, 2044
AfiI CCNNNNNNNGG 5 cut(s) 483, 1031, 1111, 1173, 1333
AflIII ACRYGT 3 cut(s) 81, 228, 654
AgsI TTSAA 7 cut(s) 32, 77, 1299, 1376, 1459, 1705, 2074
AjiI CACGTC 2 cut(s) 231, 655
AjnI CCWGG 6 cut(s) 372, 443, 476, 994, 1076, 1183
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 5 cut(s) 634, 918, 946, 984, 1371
AluI AGCT 5 cut(s) 634, 918, 946, 984, 1371
Alw21I GWGCWC 3 cut(s) 366, 636, 1428
Alw26I GTCTC 4 cut(s) 237, 299, 1015, 1856
AlwI GGATC 6 cut(s) 97, 110, 173, 772, 1930, 2030
AlwNI CAGNNNCTG 2 cut(s) 836, 977
AoxI GGCC 9 cut(s) 321, 441, 699, 1080, 1335, 1349, 1380, 1512, 1809
ApeKI GCWGC 3 cut(s) 922, 1180, 1630
Asp700I GAANNNNTTC 2 cut(s) 1407, 2100
AspLEI GCGC 3 cut(s) 560, 1635, 2050
AsuC2I CCSGG 3 cut(s) 1303, 1516, 1813
AsuHPI GGTGA 7 cut(s) 550, 584, 662, 784, 1093, 2045, 2069
AvaII GGWCC 6 cut(s) 136, 1187, 1612, 1680, 1791, 2086
AxyI CCTNAGG 2 cut(s) 2090, 2106
BaeGI GKGCMC 1 cut(s) 206
BamHI GGATCC 1 cut(s) 102
BanI GGYRCC 1 cut(s) 998
BanII GRGCYC 2 cut(s) 636, 1158
BauI CACGAG 1 cut(s) 1502
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 3 cut(s) 366, 636, 1428
BbvCI CCTCAGC 1 cut(s) 1563
BbvI GCAGC 3 cut(s) 934, 1167, 1617
BceAI ACGGC 1 cut(s) 336
BcgI CGANNNNNNTGC 2 cut(s) 1386, 1420
BciT130I CCWGG 6 cut(s) 374, 445, 478, 996, 1078, 1185
BcnI CCSGG 3 cut(s) 1303, 1516, 1813
BcoDI GTCTC 4 cut(s) 237, 299, 1015, 1856
BfaI CTAG 5 cut(s) 188, 308, 549, 707, 1547
BfmI CTRYAG 2 cut(s) 1209, 1719
BfoI RGCGCY 1 cut(s) 2051
BisI GCNGC 5 cut(s) 851, 923, 1002, 1181, 1631
BlsI GCNGC 5 cut(s) 852, 924, 1003, 1182, 1632
BmcAI AGTACT 1 cut(s) 110
Bme1390I CCNGG 9 cut(s) 374, 445, 478, 996, 1078, 1185, 1303, 1516, 1813
Bme18I GGWCC 6 cut(s) 136, 1187, 1612, 1680, 1791, 2086
BmgBI CACGTC 2 cut(s) 231, 655
BmiI GGNNCC 8 cut(s) 104, 137, 1000, 1350, 1613, 1681, 1810, 1959
BmrFI CCNGG 9 cut(s) 374, 445, 478, 996, 1078, 1185, 1303, 1516, 1813
BmrI ACTGGG 2 cut(s) 44, 289
BmsI GCATC 6 cut(s) 661, 1167, 1191, 1488, 1714, 1770
BmuI ACTGGG 2 cut(s) 44, 289
BpiI GAAGAC 1 cut(s) 84
BplI GAGNNNNNCTC 2 cut(s) 1140, 1172
BpmI CTGGAG 2 cut(s) 356, 926
Bpu10I CCTNAGC 1 cut(s) 1563
BpuEI CTTGAG 2 cut(s) 970, 2010
BpuMI CCSGG 3 cut(s) 1303, 1516, 1813
BsaHI GRCGYC 1 cut(s) 621
BsaI GGTCTC 1 cut(s) 1015
BsaJI CCNNGG 6 cut(s) 444, 477, 848, 1053, 1077, 1515
BsaWI WCCGGW 1 cut(s) 1788
BsaXI ACNNNNNCTCC 2 cut(s) 867, 897
Bsc4I CCNNNNNNNGG 5 cut(s) 483, 1031, 1111, 1173, 1333
Bse1I ACTGG 5 cut(s) 39, 106, 284, 1383, 2007
Bse21I CCTNAGG 2 cut(s) 2090, 2106
Bse3DI GCAATG 1 cut(s) 1645
BseBI CCWGG 6 cut(s) 374, 445, 478, 996, 1078, 1185
BseDI CCNNGG 6 cut(s) 444, 477, 848, 1053, 1077, 1515
BseLI CCNNNNNNNGG 5 cut(s) 483, 1031, 1111, 1173, 1333
BseMI GCAATG 1 cut(s) 1645
BseMII CTCAG 5 cut(s) 48, 581, 779, 1554, 1917
BseNI ACTGG 5 cut(s) 39, 106, 284, 1383, 2007
BseRI GAGGAG 2 cut(s) 1127, 1289
BseSI GKGCMC 1 cut(s) 206
BseXI GCAGC 3 cut(s) 934, 1167, 1617
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 9 cut(s) 323, 443, 701, 1082, 1337, 1351, 1382, 1514, 1811
BshNI GGYRCC 1 cut(s) 998
BsiHKAI GWGCWC 3 cut(s) 366, 636, 1428
BsiSI CCGG 4 cut(s) 1302, 1515, 1789, 1812
BslI CCNNNNNNNGG 5 cut(s) 483, 1031, 1111, 1173, 1333
BsmAI GTCTC 4 cut(s) 237, 299, 1015, 1856
BsmBI CGTCTC 1 cut(s) 237
BsnI GGCC 9 cut(s) 323, 443, 701, 1082, 1337, 1351, 1382, 1514, 1811
Bso31I GGTCTC 1 cut(s) 1015
Bsp1286I GDGCHC 5 cut(s) 206, 366, 636, 1158, 1428
Bsp143I GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
BspACI CCGC 7 cut(s) 535, 744, 848, 850, 1002, 1035, 1105
BspANI GGCC 9 cut(s) 323, 443, 701, 1082, 1337, 1351, 1382, 1514, 1811
BspCNI CTCAG 5 cut(s) 49, 582, 780, 1555, 1918
BspFNI CGCG 1 cut(s) 850
BspHI TCATGA 2 cut(s) 516, 604
BspLI GGNNCC 8 cut(s) 104, 137, 1000, 1350, 1613, 1681, 1810, 1959
BspPI GGATC 6 cut(s) 97, 110, 173, 772, 1930, 2030
BspQI GCTCTTC 1 cut(s) 371
BspT107I GGYRCC 1 cut(s) 998
BspTNI GGTCTC 1 cut(s) 1015
BsrDI GCAATG 1 cut(s) 1645
BsrI ACTGG 5 cut(s) 39, 106, 284, 1383, 2007
BssECI CCNNGG 6 cut(s) 444, 477, 848, 1053, 1077, 1515
BssMI GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
BssNI GRCGYC 1 cut(s) 621
BssSI CACGAG 1 cut(s) 1502
BssT1I CCWWGG 1 cut(s) 1053
Bst2BI CACGAG 1 cut(s) 1502
Bst2UI CCWGG 6 cut(s) 374, 445, 478, 996, 1078, 1185
Bst6I CTCTTC 3 cut(s) 371, 906, 2069
BstACI GRCGYC 1 cut(s) 621
BstAPI GCANNNNNTGC 1 cut(s) 977
BstC8I GCNNGC 5 cut(s) 183, 920, 982, 1353, 1600
BstDSI CCRYGG 1 cut(s) 848
BstFNI CGCG 1 cut(s) 850
BstH2I RGCGCY 1 cut(s) 2051
BstHHI GCGC 3 cut(s) 560, 1635, 2050
BstKTI GATC 9 cut(s) 105, 168, 780, 794, 1546, 1825, 1925, 1984, 2038
BstMAI GTCTC 4 cut(s) 237, 299, 1015, 1856
BstMBI GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
BstMWI GCNNNNNNNGC 2 cut(s) 707, 977
BstNI CCWGG 6 cut(s) 374, 445, 478, 996, 1078, 1185
BstNSI RCATGY 3 cut(s) 85, 1076, 1198
BstSCI CCNGG 9 cut(s) 372, 443, 476, 994, 1076, 1183, 1301, 1514, 1811
BstSFI CTRYAG 2 cut(s) 1209, 1719
BstSLI GKGCMC 1 cut(s) 206
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 3 cut(s) 934, 1167, 1617
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 3 cut(s) 102, 777, 1922
BstXI CCANNNNNNTGG 1 cut(s) 1968
BstYI RGATCY 3 cut(s) 102, 777, 1922
Bsu36I CCTNAGG 2 cut(s) 2090, 2106
BsuRI GGCC 9 cut(s) 323, 443, 701, 1082, 1337, 1351, 1382, 1514, 1811
BtgI CCRYGG 1 cut(s) 848
BtrI CACGTC 2 cut(s) 231, 655
BtsIMutI CAGTG 1 cut(s) 1686
Cac8I GCNNGC 5 cut(s) 183, 920, 982, 1353, 1600
CaiI CAGNNNCTG 2 cut(s) 836, 977
CciI TCATGA 2 cut(s) 516, 604
CfoI GCGC 3 cut(s) 560, 1635, 2050
Cfr42I CCGCGG 1 cut(s) 851
CseI GACGC 2 cut(s) 227, 270
Csp6I GTAC 4 cut(s) 109, 131, 1390, 2043
CspCI CAANNNNNGTGG 4 cut(s) 34, 69, 699, 734
CviQI GTAC 4 cut(s) 109, 131, 1390, 2043
DpnI GATC 9 cut(s) 104, 167, 779, 793, 1545, 1824, 1924, 1983, 2037
DpnII GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
EaeI YGGCCR 1 cut(s) 699
Eam1104I CTCTTC 3 cut(s) 371, 906, 2069
EarI CTCTTC 3 cut(s) 371, 906, 2069
Ecl136II GAGCTC 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 1053
Eco147I AGGCCT 1 cut(s) 1337
Eco24I GRGCYC 2 cut(s) 636, 1158
Eco31I GGTCTC 1 cut(s) 1015
Eco47I GGWCC 6 cut(s) 136, 1187, 1612, 1680, 1791, 2086
Eco53kI GAGCTC 1 cut(s) 634
Eco57I CTGAAG 1 cut(s) 140
Eco81I CCTNAGG 2 cut(s) 2090, 2106
EcoICRI GAGCTC 1 cut(s) 634
EcoO109I RGGNCCY 1 cut(s) 1187
EcoRII CCWGG 6 cut(s) 372, 443, 476, 994, 1076, 1183
EcoT14I CCWWGG 1 cut(s) 1053
EcoT22I ATGCAT 1 cut(s) 1621
EcoT38I GRGCYC 2 cut(s) 636, 1158
ErhI CCWWGG 1 cut(s) 1053
Esp3I CGTCTC 1 cut(s) 237
FauI CCCGC 2 cut(s) 528, 1112
FauNDI CATATG 1 cut(s) 490
Fnu4HI GCNGC 5 cut(s) 851, 923, 1002, 1181, 1631
FriOI GRGCYC 2 cut(s) 636, 1158
Fsp4HI GCNGC 5 cut(s) 851, 923, 1002, 1181, 1631
FspBI CTAG 5 cut(s) 188, 308, 549, 707, 1547
GlaI GCGC 3 cut(s) 559, 1634, 2049
GluI GCNGC 5 cut(s) 851, 923, 1002, 1181, 1631
GsuI CTGGAG 2 cut(s) 356, 926
HaeII RGCGCY 1 cut(s) 2051
HaeIII GGCC 9 cut(s) 323, 443, 701, 1082, 1337, 1351, 1382, 1514, 1811
HapII CCGG 4 cut(s) 1302, 1515, 1789, 1812
HgaI GACGC 2 cut(s) 227, 270
HhaI GCGC 3 cut(s) 560, 1635, 2050
Hin1I GRCGYC 1 cut(s) 621
Hin6I GCGC 3 cut(s) 558, 1633, 2048
HinP1I GCGC 3 cut(s) 558, 1633, 2048
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HpaII CCGG 4 cut(s) 1302, 1515, 1789, 1812
HphI GGTGA 7 cut(s) 550, 584, 662, 784, 1093, 2045, 2069
Hpy166II GTNNAC 3 cut(s) 226, 826, 1089
Hpy188III TCNNGA 9 cut(s) 163, 251, 388, 517, 605, 905, 1412, 1547, 1640
Hpy8I GTNNAC 3 cut(s) 226, 826, 1089
Hpy99I CGWCG 2 cut(s) 155, 623
HpyCH4IV ACGT 5 cut(s) 51, 230, 621, 654, 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 707, 977
HpySE526I ACGT 5 cut(s) 51, 230, 621, 654, 822
Hsp92I GRCGYC 1 cut(s) 621
HspAI GCGC 3 cut(s) 558, 1633, 2048
KspI CCGCGG 1 cut(s) 851
Kzo9I GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
LguI GCTCTTC 1 cut(s) 371
LmnI GCTCC 4 cut(s) 631, 1148, 1423, 1957
Lsp1109I GCAGC 3 cut(s) 934, 1167, 1617
LweI GCATC 6 cut(s) 661, 1167, 1191, 1488, 1714, 1770
MaeI CTAG 5 cut(s) 188, 308, 549, 707, 1547
MaeII ACGT 5 cut(s) 51, 230, 621, 654, 822
MaeIII GTNAC 7 cut(s) 86, 316, 650, 1099, 1264, 1882, 2020
MalI GATC 9 cut(s) 104, 167, 779, 793, 1545, 1824, 1924, 1983, 2037
MboI GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
MfeI CAATTG 3 cut(s) 510, 1795, 2052
MflI RGATCY 3 cut(s) 102, 777, 1922
MhlI GDGCHC 5 cut(s) 206, 366, 636, 1158, 1428
MluCI AATT 5 cut(s) 510, 1237, 1474, 1795, 2052
MlyI GAGTC 5 cut(s) 305, 514, 576, 1579, 2069
MmeI TCCRAC 3 cut(s) 609, 805, 1154
Mph1103I ATGCAT 1 cut(s) 1621
MroXI GAANNNNTTC 2 cut(s) 1407, 2100
MseI TTAA 2 cut(s) 501, 1773
MslI CAYNNNNRTG 2 cut(s) 1199, 1247
MspA1I CMGCKG 1 cut(s) 850
MspI CCGG 4 cut(s) 1302, 1515, 1789, 1812
MspR9I CCNGG 9 cut(s) 374, 445, 478, 996, 1078, 1185, 1303, 1516, 1813
MunI CAATTG 3 cut(s) 510, 1795, 2052
MvaI CCWGG 6 cut(s) 374, 445, 478, 996, 1078, 1185
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 2 cut(s) 707, 977
NciI CCSGG 3 cut(s) 1303, 1516, 1813
NdeI CATATG 1 cut(s) 490
NdeII GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
NlaIV GGNNCC 8 cut(s) 104, 137, 1000, 1350, 1613, 1681, 1810, 1959
NmeAIII GCCGAG 1 cut(s) 380
NmuCI GTSAC 4 cut(s) 86, 316, 650, 1099
NsiI ATGCAT 1 cut(s) 1621
NspI RCATGY 3 cut(s) 85, 1076, 1198
PagI TCATGA 2 cut(s) 516, 604
PceI AGGCCT 1 cut(s) 1337
PciI ACATGT 1 cut(s) 81
PciSI GCTCTTC 1 cut(s) 371
PdmI GAANNNNTTC 2 cut(s) 1407, 2100
PfeI GAWTC 7 cut(s) 238, 685, 878, 1049, 1306, 1403, 1455
PflFI GACNNNGTC 1 cut(s) 1588
PflMI CCANNNNNTGG 1 cut(s) 1173
PkrI GCNGC 5 cut(s) 852, 924, 1003, 1182, 1632
PleI GAGTC 5 cut(s) 305, 513, 576, 1579, 2068
PpsI GAGTC 5 cut(s) 305, 513, 576, 1579, 2068
PpuMI RGGWCCY 1 cut(s) 1187
PscI ACATGT 1 cut(s) 81
Psp124BI GAGCTC 1 cut(s) 636
Psp1406I AACGTT 1 cut(s) 822
Psp5II RGGWCCY 1 cut(s) 1187
Psp6I CCWGG 6 cut(s) 372, 443, 476, 994, 1076, 1183
PspGI CCWGG 6 cut(s) 372, 443, 476, 994, 1076, 1183
PspN4I GGNNCC 8 cut(s) 104, 137, 1000, 1350, 1613, 1681, 1810, 1959
PspPPI RGGWCCY 1 cut(s) 1187
PstNI CAGNNNCTG 2 cut(s) 836, 977
PsuI RGATCY 3 cut(s) 102, 777, 1922
PsyI GACNNNGTC 1 cut(s) 1588
RsaI GTAC 4 cut(s) 110, 132, 1391, 2044
RsaNI GTAC 4 cut(s) 109, 131, 1390, 2043
RseI CAYNNNNRTG 2 cut(s) 1199, 1247
SacI GAGCTC 1 cut(s) 636
SacII CCGCGG 1 cut(s) 851
SapI GCTCTTC 1 cut(s) 371
SaqAI TTAA 2 cut(s) 501, 1773
SatI GCNGC 5 cut(s) 851, 923, 1002, 1181, 1631
Sau3AI GATC 9 cut(s) 102, 165, 777, 791, 1543, 1822, 1922, 1981, 2035
ScaI AGTACT 1 cut(s) 110
SchI GAGTC 5 cut(s) 305, 514, 576, 1579, 2069
ScrFI CCNGG 9 cut(s) 374, 445, 478, 996, 1078, 1185, 1303, 1516, 1813
SduI GDGCHC 5 cut(s) 206, 366, 636, 1158, 1428
SfaNI GCATC 6 cut(s) 661, 1167, 1191, 1488, 1714, 1770
SfcI CTRYAG 2 cut(s) 1209, 1719
Sfr303I CCGCGG 1 cut(s) 851
SgrBI CCGCGG 1 cut(s) 851
SinI GGWCC 6 cut(s) 136, 1187, 1612, 1680, 1791, 2086
SmiMI CAYNNNNRTG 2 cut(s) 1199, 1247
SmlI CTYRAG 2 cut(s) 985, 2025
SmoI CTYRAG 2 cut(s) 985, 2025
Sse9I AATT 5 cut(s) 510, 1237, 1474, 1795, 2052
SseBI AGGCCT 1 cut(s) 1337
SsiI CCGC 7 cut(s) 535, 744, 848, 850, 1002, 1035, 1105
SspMI CTAG 5 cut(s) 188, 308, 549, 707, 1547
SstI GAGCTC 1 cut(s) 636
StuI AGGCCT 1 cut(s) 1337
StyD4I CCNGG 9 cut(s) 372, 443, 476, 994, 1076, 1183, 1301, 1514, 1811
StyI CCWWGG 1 cut(s) 1053
TaiI ACGT 5 cut(s) 54, 233, 624, 657, 825
TaqI TCGA 3 cut(s) 387, 618, 1749
TasI AATT 5 cut(s) 510, 1237, 1474, 1795, 2052
TatI WGTACW 2 cut(s) 108, 2042
TauI GCSGC 2 cut(s) 853, 1004
TfiI GAWTC 7 cut(s) 238, 685, 878, 1049, 1306, 1403, 1455
Tru1I TTAA 2 cut(s) 501, 1773
Tru9I TTAA 2 cut(s) 501, 1773
TscAI CASTG 1 cut(s) 1693
TseFI GTSAC 4 cut(s) 86, 316, 650, 1099
TseI GCWGC 3 cut(s) 922, 1180, 1630
Tsp45I GTSAC 4 cut(s) 86, 316, 650, 1099
TspDTI ATGAA 7 cut(s) 401, 593, 944, 1237, 1484, 1952, 2013
TspGWI ACGGA 1 cut(s) 2108
TspRI CASTG 1 cut(s) 1693
Tth111I GACNNNGTC 1 cut(s) 1588
Van91I CCANNNNNTGG 1 cut(s) 1173
VpaK11BI GGWCC 6 cut(s) 136, 1187, 1612, 1680, 1791, 2086
XbaI TCTAGA 1 cut(s) 1546
XceI RCATGY 3 cut(s) 85, 1076, 1198
XcmI CCANNNNNNNNNTGG 2 cut(s) 332, 441
XmnI GAANNNNTTC 2 cut(s) 1407, 2100
XspI CTAG 5 cut(s) 188, 308, 549, 707, 1547
ZraI GACGTC 1 cut(s) 622
ZrmI AGTACT 1 cut(s) 110
Zsp2I ATGCAT 1 cut(s) 1621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.