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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 133 |
| AfaI | GTAC | 1 cut(s) | 179 |
| AflIII | ACRYGT | 1 cut(s) | 84 |
| AgeI | ACCGGT | 1 cut(s) | 153 |
| AgsI | TTSAA | 3 cut(s) | 63, 160, 259 |
| AluBI | AGCT | 1 cut(s) | 21 |
| AluI | AGCT | 1 cut(s) | 21 |
| Alw21I | GWGCWC | 1 cut(s) | 129 |
| Alw26I | GTCTC | 1 cut(s) | 45 |
| ApeKI | GCWGC | 1 cut(s) | 21 |
| AsiGI | ACCGGT | 1 cut(s) | 153 |
| AspS9I | GGNCC | 1 cut(s) | 151 |
| AvaII | GGWCC | 1 cut(s) | 151 |
| Bbv12I | GWGCWC | 1 cut(s) | 129 |
| BbvI | GCAGC | 1 cut(s) | 8 |
| BccI | CCATC | 1 cut(s) | 201 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 84, 118 |
| BcoDI | GTCTC | 1 cut(s) | 45 |
| BisI | GCNGC | 1 cut(s) | 22 |
| BlsI | GCNGC | 1 cut(s) | 23 |
| Bme18I | GGWCC | 1 cut(s) | 151 |
| BmgT120I | GGNCC | 1 cut(s) | 151 |
| BmsI | GCATC | 2 cut(s) | 321, 324 |
| BsaAI | YACGTR | 2 cut(s) | 10, 87 |
| BsaWI | WCCGGW | 1 cut(s) | 153 |
| Bse118I | RCCGGY | 1 cut(s) | 153 |
| Bse3DI | GCAATG | 1 cut(s) | 202 |
| BseGI | GGATG | 3 cut(s) | 56, 258, 321 |
| BseMI | GCAATG | 1 cut(s) | 202 |
| BseRI | GAGGAG | 1 cut(s) | 86 |
| BseXI | GCAGC | 1 cut(s) | 8 |
| BshTI | ACCGGT | 1 cut(s) | 153 |
| BsiHKAI | GWGCWC | 1 cut(s) | 129 |
| BsiSI | CCGG | 1 cut(s) | 154 |
| BsmAI | GTCTC | 1 cut(s) | 45 |
| BsmBI | CGTCTC | 1 cut(s) | 45 |
| Bsp1286I | GDGCHC | 1 cut(s) | 129 |
| BspACI | CCGC | 1 cut(s) | 133 |
| BsrDI | GCAATG | 1 cut(s) | 202 |
| BsrFI | RCCGGY | 1 cut(s) | 153 |
| BssAI | RCCGGY | 1 cut(s) | 153 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 141 |
| BstBAI | YACGTR | 2 cut(s) | 10, 87 |
| BstDEI | CTNAG | 1 cut(s) | 348 |
| BstF5I | GGATG | 3 cut(s) | 56, 258, 321 |
| BstMAI | GTCTC | 1 cut(s) | 45 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 141 |
| BstSNI | TACGTA | 1 cut(s) | 10 |
| BstV1I | GCAGC | 1 cut(s) | 8 |
| BtsCI | GGATG | 3 cut(s) | 56, 258, 321 |
| BtsIMutI | CAGTG | 1 cut(s) | 240 |
| Cfr10I | RCCGGY | 1 cut(s) | 153 |
| Cfr13I | GGNCC | 1 cut(s) | 151 |
| CseI | GACGC | 1 cut(s) | 101 |
| Csp6I | GTAC | 1 cut(s) | 178 |
| CspAI | ACCGGT | 1 cut(s) | 153 |
| CviAII | CATG | 2 cut(s) | 263, 331 |
| CviJI | RGCY | 3 cut(s) | 21, 187, 229 |
| CviKI_1 | RGCY | 3 cut(s) | 21, 187, 229 |
| CviQI | GTAC | 1 cut(s) | 178 |
| DdeI | CTNAG | 1 cut(s) | 348 |
| Eco105I | TACGTA | 1 cut(s) | 10 |
| Eco47I | GGWCC | 1 cut(s) | 151 |
| Esp3I | CGTCTC | 1 cut(s) | 45 |
| FaeI | CATG | 2 cut(s) | 266, 334 |
| FaiI | YATR | 6 cut(s) | 35, 67, 69, 90, 264, 332 |
| FatI | CATG | 2 cut(s) | 262, 330 |
| FauI | CCCGC | 1 cut(s) | 140 |
| FauNDI | CATATG | 1 cut(s) | 67 |
| Fnu4HI | GCNGC | 1 cut(s) | 22 |
| FokI | GGATG | 3 cut(s) | 63, 265, 308 |
| Fsp4HI | GCNGC | 1 cut(s) | 22 |
| GluI | GCNGC | 1 cut(s) | 22 |
| HapII | CCGG | 1 cut(s) | 154 |
| HgaI | GACGC | 1 cut(s) | 101 |
| Hin1II | CATG | 2 cut(s) | 266, 334 |
| HinfI | GANTC | 2 cut(s) | 29, 101 |
| HpaII | CCGG | 1 cut(s) | 154 |
| Hpy188I | TCNGA | 1 cut(s) | 28 |
| Hpy188III | TCNNGA | 5 cut(s) | 113, 149, 197, 272, 325 |
| HpyAV | CCTTC | 1 cut(s) | 70 |
| HpyCH4IV | ACGT | 2 cut(s) | 9, 86 |
| HpyCH4V | TGCA | 2 cut(s) | 144, 337 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 141 |
| HpyF3I | CTNAG | 1 cut(s) | 348 |
| HpySE526I | ACGT | 2 cut(s) | 9, 86 |
| Hsp92II | CATG | 2 cut(s) | 266, 334 |
| LmnI | GCTCC | 1 cut(s) | 29 |
| LpnPI | CCDG | 5 cut(s) | 81, 134, 167, 285, 338 |
| Lsp1109I | GCAGC | 1 cut(s) | 8 |
| LweI | GCATC | 2 cut(s) | 321, 324 |
| MaeII | ACGT | 2 cut(s) | 9, 86 |
| MboII | GAAGA | 2 cut(s) | 232, 307 |
| MhlI | GDGCHC | 1 cut(s) | 129 |
| MlyI | GAGTC | 2 cut(s) | 23, 110 |
| MmeI | TCCRAC | 3 cut(s) | 51, 150, 308 |
| MnlI | CCTC | 3 cut(s) | 64, 177, 219 |
| MseI | TTAA | 1 cut(s) | 342 |
| MspA1I | CMGCKG | 1 cut(s) | 21 |
| MspI | CCGG | 1 cut(s) | 154 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 141 |
| NdeI | CATATG | 1 cut(s) | 67 |
| NlaIII | CATG | 2 cut(s) | 266, 334 |
| PinAI | ACCGGT | 1 cut(s) | 153 |
| PkrI | GCNGC | 1 cut(s) | 23 |
| PleI | GAGTC | 2 cut(s) | 23, 109 |
| PpsI | GAGTC | 2 cut(s) | 23, 109 |
| Ppu21I | YACGTR | 2 cut(s) | 10, 87 |
| PspPI | GGNCC | 1 cut(s) | 151 |
| PvuII | CAGCTG | 1 cut(s) | 21 |
| RsaI | GTAC | 1 cut(s) | 179 |
| RsaNI | GTAC | 1 cut(s) | 178 |
| SaqAI | TTAA | 1 cut(s) | 342 |
| SatI | GCNGC | 1 cut(s) | 22 |
| Sau96I | GGNCC | 1 cut(s) | 151 |
| SchI | GAGTC | 2 cut(s) | 23, 110 |
| SduI | GDGCHC | 1 cut(s) | 129 |
| SetI | ASST | 5 cut(s) | 12, 23, 89, 238, 365 |
| SfaNI | GCATC | 2 cut(s) | 321, 324 |
| SinI | GGWCC | 1 cut(s) | 151 |
| SnaBI | TACGTA | 1 cut(s) | 10 |
| SsiI | CCGC | 1 cut(s) | 133 |
| TaiI | ACGT | 2 cut(s) | 12, 89 |
| TatI | WGTACW | 1 cut(s) | 177 |
| Tru1I | TTAA | 1 cut(s) | 342 |
| Tru9I | TTAA | 1 cut(s) | 342 |
| TscAI | CASTG | 1 cut(s) | 247 |
| TseI | GCWGC | 1 cut(s) | 21 |
| TspGWI | ACGGA | 1 cut(s) | 63 |
| TspRI | CASTG | 1 cut(s) | 247 |
| VpaK11BI | GGWCC | 1 cut(s) | 151 |