Rmu_sc0003179.1_g000023

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003179.1
OG0000124
Physical Location & Seq
Reverse (-)
104343 .. 105793
1451 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003179.1_g000023.1.cds

Sequence Viewer

Length: 942 bp
atggagcatcacctcgacgtcaactacccatatttctcagtgatggttcggctgccgactcagaacgtggacaaggcggtgagttgtattgttgtttatgaattcgaaaattcctccggcgtttatggagaggcacatgaggtactttaccgggatgcggatcgagctgcgtttgtccggactgaggcgggttccgtaccgcatctgcctgacagcacaaggggatcaccggtggatgcacggaagttggaaccccaaaaccccccagtgcccttccactccttcgtctccgacgtctgggtcgaggatgtcactgtgataagtcccgccatcccaaggggtcgcctatacgctgccatccgcaatgatggcggtccaggaatgtgtctcttaaggtgggagtcaagggaggggatgatattctggcatgttgagctgactagaggcgtaggttgcatgacttttggtgaagggtggaacagatttgcatcacacaatcggctgcttgatggaaagcggttttgtgtcttcacttacaggggtggccttgagttctccgaggtaaatgttgatgaggctcagcaggatatggacattggcggtgtagagggggccggtttgggggcttctagtgatgggcttgtttacgaggacgttgtcggtgactaccaacgccttcgtcattgtgatgtcaggattcagcaacgtcatggagcgtttctcacaaaagatgctcgttatctgtttcctagaaagggtaacgcatgtgttaagctgcaaatgtcagacggagccgtgtgggatatggggtttgcccatacgaacgacactcactacctgacaaggggctacagtaccttcaaggcgttcaatggactacgcacgggtcagcttctccgactctatatcgtctcaaaccggatgttcgtattcgacgtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

313

Amino Acids

35.29

Weight (kDa)

6.0

Isoelectric Point (pI)

32.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000124)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10992 FvH4_2g25150 FvH4_2g27910 FvH4_2g27931 FvH4_4g09443 FvH4_4g09490 FvH4_4g15990 FvH4_4g15991 FvH4_6g07180 FvH4_6g40320 FvH4_7g10222 FvH4_7g10251
rosa_chinensis RchiOBHm_Chr2g0155611
rosa_laevigata RLG00000009253 RLG00000009254 RLG00000011848 RLG00000017301 RLG00000017302 RLG00000034909
rosa_multiflora Rmu_co8063658.1_g000001 Rmu_co8240443.1_g000001 Rmu_co8264451.1_g000001 Rmu_co8381937.1_g000001 Rmu_co8419903.1_g000001 Rmu_co8513405.1_g000002 Rmu_co8522915.1_g000001 Rmu_co8522915.1_g000004 Rmu_sc0000279.1_g000024 Rmu_sc0000279.1_g000048 Rmu_sc0000642.1_g000017 Rmu_sc0002045.1_g000015 Rmu_sc0002045.1_g000018 Rmu_sc0002045.1_g000023 Rmu_sc0002045.1_g000028 Rmu_sc0003047.1_g000008 Rmu_sc0003179.1_g000019 Rmu_sc0003179.1_g000020 Rmu_sc0003179.1_g000022 Rmu_sc0003179.1_g000023 Rmu_sc0003422.1_g000011 Rmu_sc0004999.1_g000014 Rmu_sc0005712.1_g000018 Rmu_sc0005712.1_g000023 Rmu_sc0006198.1_g000019 Rmu_sc0006373.1_g000005 Rmu_sc0006514.1_g000028 Rmu_sc0006514.1_g000031 Rmu_sc0007367.1_g000010 Rmu_sc0011475.1_g000002 Rmu_sc0025304.1_g000001 Rmu_ssc0000161.1_g000030 Rmu_ssc0000161.1_g000032 Rmu_ssc0000387.1_g000018
rosa_roxburghii Rroxscaffold_1G00031090 Rroxscaffold_1G00032770 Rroxscaffold_1G00068950 Rroxscaffold_1G00069000 Rroxscaffold_1G00069010 Rroxscaffold_2G00129060 Rroxscaffold_2G00129100 Rroxscaffold_4G00309310 Rroxscaffold_4G00309320 Rroxscaffold_5G00350620 Rroxscaffold_5G00350630 Rroxscaffold_7G00162650 Rroxscaffold_7G00162660 Rroxscaffold_7G00162710
rosa_rugosa Rorug02G0564700 Rorug02G0564700 Rorug03G0343100 Rorug04G0189700 Rorug05G0102800 Rorug05G0102900 Rorug05G0103000 Rorug05G0106500 Rorug05G0106600 Rorug05G0106700 Rorug05G0252800 Rorug07G0182100 Rorug07G0283500 Rorug07G0283600
rosa_samantha Rh1AG004000 Rh1AG004100 Rh1AG004200 Rh1AG004400 Rh1AG004500 Rh1AG004600 Rh1AG004800 Rh1AG004900 Rh1AG005300 Rh1AG007000 Rh1AG115000 Rh1CG049400 Rh1CG055400 Rh1CG111000 Rh1DG053600 Rh1DG053700 Rh1DG059300 Rh1DG119700 Rh1DG177100 Rh1DG177200 Rh2AG353800 Rh2AG354000 Rh2AG354100 Rh2AG354300 Rh2AG354400 Rh2AG354500 Rh2AG354600 Rh2AG354700 Rh2AG642300 Rh2AG642400 Rh2AG642500 Rh2BG336500 Rh2BG336600 Rh2BG336700 Rh2BG336800 Rh2BG336900 Rh2CG299900 Rh2CG300000 Rh2CG300100 Rh2DG337000 Rh2DG337100 Rh3AG246600 Rh3BG121900 Rh3BG122300 Rh3BG122600 Rh3BG122700 Rh3DG304800 Rh3DG304900 Rh4AG148800 Rh4AG249000 Rh4AG249100 Rh4AG249200 Rh4BG182700 Rh4BG182800 Rh4BG182900 Rh4BG254400 Rh4BG254500 Rh4BG254600 Rh4CG155500 Rh4CG155700 Rh4DG057900 Rh4DG058000 Rh4DG058100 Rh4DG058200 Rh4DG129100 Rh5AG364500 Rh5AG364600 Rh5AG364800 Rh5AG365300 Rh5CG363700 Rh5CG497600 Rh5DG239500 Rh5DG239600 Rh6AG173500 Rh6AG175400 Rh6BG075900 Rh6BG076300 Rh6BG076400 Rh6BG076500 Rh6BG076600 Rh6CG151000 Rh6CG151200 Rh6CG151300 Rh6CG151400 Rh6CG205200 Rh6CG205300 Rh6CG419700 Rh6CG419900 Rh6CG420100 Rh6DG498700 Rh6DG498800 Rh7AG329000 Rh7AG329100 Rh7AG329300 Rh7AG329400 Rh7AG353000 Rh7AG353100 Rh7AG353300 Rh7AG353400 Rh7AG353500 Rh7CG346400 Rh7CG346500 Rh7CG346700 Rh7CG370800 Rh7CG370900 Rh7CG371000 Rh7CG371100 Rh7DG325000 Rh7DG325100 Rh7DG325200 Rh7DG325300 Rh7DG353100 Rh7DG353200 Rh7DG353300 Rh7DG363500 Rh7DG364000
rosa_wichuraiana Rw0G002290 Rw0G012780 Rw1G000260 Rw1G000270 Rw1G010400 Rw1G010410 Rw1G010420 Rw1G010430 Rw1G010440 Rw2G003210 Rw2G003220 Rw4G010970 Rw5G048520 Rw6G013320 Rw6G013470 Rw6G014940 Rw6G016570 Rw6G016590 Rw6G016600 Rw6G016610 Rw6G016620 Rw6G035450 Rw7G027760 Rw7G030460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AatII GACGTC 2 cut(s) 21, 297
AccIII TCCGGA 1 cut(s) 177
AciI CCGC 9 cut(s) 77, 158, 188, 200, 327, 361, 372, 517, 600
AclWI GGATC 2 cut(s) 168, 232
AcsI RAATTY 2 cut(s) 101, 109
AcyI GRCGYC 2 cut(s) 18, 294
AfaI GTAC 3 cut(s) 144, 198, 856
AfiI CCNNNNNNNGG 7 cut(s) 157, 184, 297, 336, 621, 755, 844
AflII CTTAAG 1 cut(s) 391
AgeI ACCGGT 1 cut(s) 229
AgsI TTSAA 2 cut(s) 862, 871
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 4 cut(s) 167, 436, 775, 892
AluI AGCT 4 cut(s) 167, 436, 775, 892
Alw26I GTCTC 3 cut(s) 292, 392, 916
AlwI GGATC 2 cut(s) 168, 232
Aor13HI TCCGGA 1 cut(s) 177
AoxI GGCC 2 cut(s) 544, 612
ApeKI GCWGC 5 cut(s) 52, 167, 353, 502, 775
ApoI RAATTY 2 cut(s) 101, 109
AsiGI ACCGGT 1 cut(s) 229
AspS9I GGNCC 2 cut(s) 374, 612
AsuC2I CCSGG 1 cut(s) 152
AsuHPI GGTGA 4 cut(s) 91, 219, 479, 674
AsuII TTCGAA 1 cut(s) 105
AvaII GGWCC 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 273
BbsI GAAGAC 1 cut(s) 520
BbvI GCAGC 5 cut(s) 39, 154, 340, 489, 762
BccI CCATC 6 cut(s) 37, 338, 362, 365, 503, 629
BceAI ACGGC 1 cut(s) 779
BciT130I CCWGG 1 cut(s) 378
BcnI CCSGG 1 cut(s) 152
BcoDI GTCTC 3 cut(s) 292, 392, 916
BfaI CTAG 3 cut(s) 441, 630, 750
BfmI CTRYAG 1 cut(s) 850
BfrI CTTAAG 1 cut(s) 391
BisI GCNGC 5 cut(s) 53, 168, 354, 503, 776
BlpI GCTNAGC 1 cut(s) 579
BlsI GCNGC 5 cut(s) 54, 169, 355, 504, 777
Bme1390I CCNGG 2 cut(s) 152, 378
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 2 cut(s) 374, 612
BmiI GGNNCC 4 cut(s) 193, 252, 613, 793
BmrFI CCNGG 2 cut(s) 152, 378
BmrI ACTGGG 1 cut(s) 260
BmsI GCATC 6 cut(s) 16, 145, 211, 226, 497, 721
BmuI ACTGGG 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 520
BplI GAGNNNNNCTC 2 cut(s) 705, 737
Bpu1102I GCTNAGC 1 cut(s) 579
Bpu14I TTCGAA 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 569
BpuMI CCSGG 1 cut(s) 152
BsaBI GATNNNNATC 2 cut(s) 159, 487
BsaHI GRCGYC 2 cut(s) 18, 294
BsaJI CCNNGG 2 cut(s) 335, 558
BsaWI WCCGGW 3 cut(s) 177, 229, 918
Bsc4I CCNNNNNNNGG 7 cut(s) 157, 184, 297, 336, 621, 755, 844
Bse118I RCCGGY 2 cut(s) 229, 614
Bse1I ACTGG 1 cut(s) 266
Bse3DI GCAATG 1 cut(s) 370
Bse8I GATNNNNATC 2 cut(s) 159, 487
BseAI TCCGGA 1 cut(s) 177
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 2 cut(s) 335, 558
BseGI GGATG 7 cut(s) 160, 241, 313, 330, 357, 420, 927
BseJI GATNNNNATC 2 cut(s) 159, 487
BseLI CCNNNNNNNGG 7 cut(s) 157, 184, 297, 336, 621, 755, 844
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 4 cut(s) 51, 74, 174, 593
BseNI ACTGG 1 cut(s) 266
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 5 cut(s) 39, 154, 340, 489, 762
BshFI GGCC 2 cut(s) 546, 614
BshTI ACCGGT 1 cut(s) 229
BsiSI CCGG 6 cut(s) 117, 151, 178, 230, 615, 919
BslFI GGGAC 1 cut(s) 309
BslI CCNNNNNNNGG 7 cut(s) 157, 184, 297, 336, 621, 755, 844
BsmAI GTCTC 3 cut(s) 292, 392, 916
BsmBI CGTCTC 2 cut(s) 292, 916
BsmFI GGGAC 1 cut(s) 309
BsnI GGCC 2 cut(s) 546, 614
Bsp119I TTCGAA 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 273
Bsp13I TCCGGA 1 cut(s) 177
Bsp143I GATC 2 cut(s) 160, 224
Bsp1720I GCTNAGC 1 cut(s) 579
BspACI CCGC 9 cut(s) 77, 158, 188, 200, 327, 361, 372, 517, 600
BspANI GGCC 2 cut(s) 546, 614
BspCNI CTCAG 4 cut(s) 50, 73, 175, 592
BspEI TCCGGA 1 cut(s) 177
BspLI GGNNCC 4 cut(s) 193, 252, 613, 793
BspPI GGATC 2 cut(s) 168, 232
BspT104I TTCGAA 1 cut(s) 105
BspTI CTTAAG 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 370
BsrFI RCCGGY 2 cut(s) 229, 614
BsrI ACTGG 1 cut(s) 266
BssAI RCCGGY 2 cut(s) 229, 614
BssECI CCNNGG 2 cut(s) 335, 558
BssMI GATC 2 cut(s) 160, 224
BssNI GRCGYC 2 cut(s) 18, 294
BssT1I CCWWGG 1 cut(s) 335
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 2 cut(s) 316, 854
BstACI GRCGYC 2 cut(s) 18, 294
BstAFI CTTAAG 1 cut(s) 391
BstBI TTCGAA 1 cut(s) 105
BstDEI CTNAG 4 cut(s) 37, 60, 183, 579
BstF5I GGATG 7 cut(s) 160, 241, 313, 330, 357, 420, 927
BstKTI GATC 2 cut(s) 163, 227
BstMAI GTCTC 3 cut(s) 292, 392, 916
BstMBI GATC 2 cut(s) 160, 224
BstMWI GCNNNNNNNGC 4 cut(s) 164, 369, 433, 453
BstNI CCWGG 1 cut(s) 378
BstNSI RCATGY 2 cut(s) 431, 768
BstSCI CCNGG 2 cut(s) 150, 376
BstSFI CTRYAG 1 cut(s) 850
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 5 cut(s) 39, 154, 340, 489, 762
BstV2I GAAGAC 1 cut(s) 520
BsuRI GGCC 2 cut(s) 546, 614
BtsCI GGATG 7 cut(s) 160, 241, 313, 330, 357, 420, 927
BtsIMutI CAGTG 3 cut(s) 45, 273, 312
Cfr10I RCCGGY 2 cut(s) 229, 614
Cfr13I GGNCC 2 cut(s) 374, 612
Csp6I GTAC 3 cut(s) 143, 197, 855
CspAI ACCGGT 1 cut(s) 229
CviAII CATG 5 cut(s) 137, 428, 457, 710, 765
CviQI GTAC 3 cut(s) 143, 197, 855
DdeI CTNAG 4 cut(s) 37, 60, 183, 579
DpnI GATC 2 cut(s) 162, 226
DpnII GATC 2 cut(s) 160, 224
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 335
Eco47I GGWCC 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 101
EcoRII CCWGG 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 335
Esp3I CGTCTC 2 cut(s) 292, 916
FaeI CATG 5 cut(s) 140, 431, 460, 713, 768
FaqI GGGAC 1 cut(s) 309
FatI CATG 5 cut(s) 136, 427, 456, 709, 764
FauI CCCGC 2 cut(s) 181, 334
Fnu4HI GCNGC 5 cut(s) 53, 168, 354, 503, 776
FokI GGATG 7 cut(s) 167, 248, 317, 320, 344, 427, 934
Fsp4HI GCNGC 5 cut(s) 53, 168, 354, 503, 776
FspBI CTAG 3 cut(s) 441, 630, 750
GluI GCNGC 5 cut(s) 53, 168, 354, 503, 776
HaeIII GGCC 2 cut(s) 546, 614
HapII CCGG 6 cut(s) 117, 151, 178, 230, 615, 919
Hin1I GRCGYC 2 cut(s) 18, 294
Hin1II CATG 5 cut(s) 140, 431, 460, 713, 768
HincII GTYRAC 1 cut(s) 22
HindII GTYRAC 1 cut(s) 22
HinfI GANTC 4 cut(s) 58, 401, 697, 900
HpaII CCGG 6 cut(s) 117, 151, 178, 230, 615, 919
HphI GGTGA 4 cut(s) 91, 219, 479, 674
Hpy166II GTNNAC 3 cut(s) 22, 70, 646
Hpy188I TCNGA 5 cut(s) 63, 292, 559, 787, 899
Hpy188III TCNNGA 2 cut(s) 178, 694
Hpy8I GTNNAC 3 cut(s) 22, 70, 646
Hpy99I CGWCG 3 cut(s) 20, 296, 938
HpyAV CCTTC 5 cut(s) 283, 292, 464, 686, 868
HpyCH4III ACNGT 2 cut(s) 316, 854
HpyCH4IV ACGT 6 cut(s) 18, 66, 294, 654, 706, 936
HpyCH4V TGCA 4 cut(s) 239, 456, 488, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 164, 369, 433, 453
HpyF3I CTNAG 4 cut(s) 37, 60, 183, 579
HpySE526I ACGT 6 cut(s) 18, 66, 294, 654, 706, 936
Hsp92I GRCGYC 2 cut(s) 18, 294
Hsp92II CATG 5 cut(s) 140, 431, 460, 713, 768
Kpn2I TCCGGA 1 cut(s) 177
Kzo9I GATC 2 cut(s) 160, 224
LmnI GCTCC 3 cut(s) 4, 713, 791
Lsp1109I GCAGC 5 cut(s) 39, 154, 340, 489, 762
LweI GCATC 6 cut(s) 16, 145, 211, 226, 497, 721
MaeI CTAG 3 cut(s) 441, 630, 750
MaeII ACGT 6 cut(s) 18, 66, 294, 654, 706, 936
MaeIII GTNAC 3 cut(s) 310, 662, 758
MalI GATC 2 cut(s) 162, 226
MboI GATC 2 cut(s) 160, 224
MboII GAAGA 1 cut(s) 520
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 2 cut(s) 101, 109
MlyI GAGTC 3 cut(s) 52, 410, 894
MmeI TCCRAC 3 cut(s) 228, 315, 922
MroI TCCGGA 1 cut(s) 177
MseI TTAA 2 cut(s) 392, 771
MslI CAYNNNNRTG 1 cut(s) 687
MspCI CTTAAG 1 cut(s) 391
MspI CCGG 6 cut(s) 117, 151, 178, 230, 615, 919
MspR9I CCNGG 2 cut(s) 152, 378
MvaI CCWGG 1 cut(s) 378
MwoI GCNNNNNNNGC 4 cut(s) 164, 369, 433, 453
NciI CCSGG 1 cut(s) 152
NdeII GATC 2 cut(s) 160, 224
NlaIII CATG 5 cut(s) 140, 431, 460, 713, 768
NlaIV GGNNCC 4 cut(s) 193, 252, 613, 793
NmuCI GTSAC 2 cut(s) 310, 662
NspI RCATGY 2 cut(s) 431, 768
NspV TTCGAA 1 cut(s) 105
PcsI WCGNNNNNNNCGW 3 cut(s) 291, 300, 933
PfeI GAWTC 1 cut(s) 697
PflFI GACNNNGTC 1 cut(s) 656
PfoI TCCNGGA 1 cut(s) 376
PinAI ACCGGT 1 cut(s) 229
PkrI GCNGC 5 cut(s) 54, 169, 355, 504, 777
PleI GAGTC 3 cut(s) 52, 409, 894
PpsI GAGTC 3 cut(s) 52, 409, 894
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 4 cut(s) 193, 252, 613, 793
PspPI GGNCC 2 cut(s) 374, 612
PsyI GACNNNGTC 1 cut(s) 656
RsaI GTAC 3 cut(s) 144, 198, 856
RsaNI GTAC 3 cut(s) 143, 197, 855
RseI CAYNNNNRTG 1 cut(s) 687
SaqAI TTAA 2 cut(s) 392, 771
SatI GCNGC 5 cut(s) 53, 168, 354, 503, 776
Sau3AI GATC 2 cut(s) 160, 224
Sau96I GGNCC 2 cut(s) 374, 612
SchI GAGTC 3 cut(s) 52, 410, 894
ScrFI CCNGG 2 cut(s) 152, 378
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 6 cut(s) 16, 145, 211, 226, 497, 721
SfcI CTRYAG 1 cut(s) 850
SfuI TTCGAA 1 cut(s) 105
SgrAI CRCCGGYG 1 cut(s) 229
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 687
SmlI CTYRAG 2 cut(s) 391, 548
SmoI CTYRAG 2 cut(s) 391, 548
Sse9I AATT 2 cut(s) 101, 109
SsiI CCGC 9 cut(s) 77, 158, 188, 200, 327, 361, 372, 517, 600
SspMI CTAG 3 cut(s) 441, 630, 750
StyD4I CCNGG 2 cut(s) 150, 376
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 2 cut(s) 316, 854
TaiI ACGT 6 cut(s) 21, 69, 297, 657, 709, 939
TaqI TCGA 5 cut(s) 15, 105, 163, 303, 933
TasI AATT 2 cut(s) 101, 109
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 2 cut(s) 392, 771
Tru9I TTAA 2 cut(s) 392, 771
TscAI CASTG 3 cut(s) 45, 273, 319
TseFI GTSAC 2 cut(s) 310, 662
TseI GCWGC 5 cut(s) 52, 167, 353, 502, 775
Tsp45I GTSAC 2 cut(s) 310, 662
TspDTI ATGAA 1 cut(s) 114
TspGWI ACGGA 3 cut(s) 184, 256, 804
TspRI CASTG 3 cut(s) 45, 273, 319
Tth111I GACNNNGTC 1 cut(s) 656
Vha464I CTTAAG 1 cut(s) 391
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 2 cut(s) 101, 109
XceI RCATGY 2 cut(s) 431, 768
XspI CTAG 3 cut(s) 441, 630, 750
ZraI GACGTC 2 cut(s) 19, 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.