Rh2AG354400

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000124
Physical Location & Seq
Forward (+)
52309076 .. 52314567
5492 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG354400.1

Sequence Viewer

Length: 2406 bp
ATGACGCGACACGGTTGCATGGTGAGGGTGACACTGCAAATGGAACCGAAAAAATGTTGGGTTAATGCATGCAATGGTTCAAGCGGAGAAGGTTGTGCGCGGTGGGTGTCGGCTAGGCGTAAGCTTGACGTGGGGAGAGGGAGGATTGAGACTGGGTTACAACTCCCTGGCAACCTTTCCACTGCTGCGGGGAAGGATGAACAAAAAATGACATTATCTAAGATTGGCGAGTTCAAGGACACCGTGAACAACCTATCAGATGCAAAAGTTAAGGCCGTTGTGGGGGTGGGATATGGATTTATGCAATGCATTAGGGTAGATGACGTCGTCCCCGAAATTTTTCGAGACATGGTCAAAAATATCGACACGGAAGCATGCAATATCAGGCTGAAGGGTAGGCTTCTGTCTGTAGACGCGGACGAATTTACACGTGTTATGGGCATTCACAACGGGGGGCATGCCATTGAACTCGGGGGTGACATTAGTCCTGCTGAATATGGAAATGTTGTAGCTCCGATGCTAGGCCCAGTTGGCAATATAGCAGTGGACAATCTGAAGTTCATTGTGCGTGACAGGGAAATTGTTGACGACTGTTTCATTATAGCTTATGTATTGTTGGCTCTGAGCCTAGTAATATGTCCGGGAACAAGTAATGATGTACAAAGTGGGTTGTTGTTGCCATTGCTAGATACCCGCAGTATAGGAGCGAAGAATTGGGCAACATTTGCAGTGGACTACTTGATGGAGGGAATAAGAAAGCATCGTCAAGAAGGATCCCCGGTTGTAGCGGGATGTGCACTATATCTACAGCTAGTATATTGTGACAAAGTTGGTGAGAAGTTACTGCCAAAGGAGCGGCTAGTGGCACCTGTGATGAATTGGAGCCTTGGGGAGATAAATGTGTTGCTGGGTAGGTTAGTAGCAGAAACTAAAACCAAGATAATGGGTGAGACGGGGGACAATTGTAATAAGGGGACTAGTGTGGATGAGGCAGTGAAAGACGAAGAGTCTGTGGGATTTCCATTAAGAAAATCACTACACGAAGACATGTTTGATCTTATGGTCGACGTGGGAAGGTTAAAAGATGATATGGTGGTTGTGAAAGAAATCGTAACCGGAATACCGCTGAAATTTTCGGAGCTAACAGAAGGTGTGTTGGTGCTTTTGGAATCTTTATTGAAGGTGAAGGAAACTTGTGTTGCGGAGGTTAGAGAGGAGTGGCGATTCATGCGGATGAACATGCAAGATACGGAGCAACGACATGAAGGGGAGGACTATTGTAGCCCACATCGCCATGCAGTAGAGCAAAGAATTGAACAGGGGCAAAACGTTATTATAGAAGAAGTACATGGCCATGAAAAGGCGGTTCGGTCGGACACTGGAAGCAGCCCAACCGGAGCACAATGTCGGAAAAGAAAAAGCTTCAGGGAAGAAGGCACCATTAAAGACAAGGGTGTGAGGCGAAGTCATGGACGTGCTGTGGGTGAAAACAGAAGTGATAAGTATGACTACATCAAGTATGTAATGGGCTATACGGGAAAGGAAGACGAAACTTCCAATTCGATGGGACGAATGGTTGCCGGGCCGTACAGACTGAAGGTCACATTACCAGATTTCGACGTCGCACTGATAGAATTTGTTTTTTTGGGGGGAAAGGTCCTGACATCAGAAGAAATCAGAACATGGATCGGGGTGTGCGGAGATGGGGCAGAAGTTATTTATAGAGGGGACTTGGTCACCCTAAACCCTTGTGTGTGGGTATCGGGAAGTTATAGAGCTACACATAATGAGGATAACACATGTTTCGACTGGGTGAACCACACAAGGGAAGTATGCAAGTTGGAGAGGTTCGACGAGAAGATTGCAGAGTGCGAGAAGATTTTTTTGCCAGTATATGAAGGGGAACATAACAATGGACATTGGTTCCTTATTGTGGTAGACATGGTCAAAAGGGTGGCTGAATTATGGGATAGTGGACCTGGAAAGCGACTAACGGCGGGGCGACTATTAACGATGTCCCAAGTGCTACAACATATTGACCGTGTGATGACGGTTGAGAACTTAAAGCAAAATGGAGGAGGGAAAACAGTCTCATATTTTGTGACTGTTGAACCGCATCGTGTACCCAAACAAAGGAATTCATATGACTGTGGGGTGTACGTTATGAAGAACATGCAAAACTATGGAGACGATTGGGCATTAGGGATGTCAACAACAATAGAAGATGTCATCAAAGGAAGTTGCTATGGGGTGGAGGATCGACTGACTCAAGTAAGGGGGGAGCAGACCCCTGGTATAGCTCATCCATTTGAAGAATGGGGTTCTGATCAGTTTCACATGTTTGATAAAGCCGGAGATTGGATTGGAGGTGGCGAAGAAGGGGGAACAGAAACCAGAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 Peptidase_C48

Protein Analysis

801

Amino Acids

89.44

Weight (kDa)

5.61

Isoelectric Point (pI)

32.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 598 - 726 1.6e-14 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000124)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10992 FvH4_2g25150 FvH4_2g27910 FvH4_2g27931 FvH4_4g09443 FvH4_4g09490 FvH4_4g15990 FvH4_4g15991 FvH4_6g07180 FvH4_6g40320 FvH4_7g10222 FvH4_7g10251
rosa_chinensis RchiOBHm_Chr2g0155611
rosa_laevigata RLG00000009253 RLG00000009254 RLG00000011848 RLG00000017301 RLG00000017302 RLG00000034909
rosa_multiflora Rmu_co8063658.1_g000001 Rmu_co8240443.1_g000001 Rmu_co8264451.1_g000001 Rmu_co8381937.1_g000001 Rmu_co8419903.1_g000001 Rmu_co8513405.1_g000002 Rmu_co8522915.1_g000001 Rmu_co8522915.1_g000004 Rmu_sc0000279.1_g000024 Rmu_sc0000279.1_g000048 Rmu_sc0000642.1_g000017 Rmu_sc0002045.1_g000015 Rmu_sc0002045.1_g000018 Rmu_sc0002045.1_g000023 Rmu_sc0002045.1_g000028 Rmu_sc0003047.1_g000008 Rmu_sc0003179.1_g000019 Rmu_sc0003179.1_g000020 Rmu_sc0003179.1_g000022 Rmu_sc0003179.1_g000023 Rmu_sc0003422.1_g000011 Rmu_sc0004999.1_g000014 Rmu_sc0005712.1_g000018 Rmu_sc0005712.1_g000023 Rmu_sc0006198.1_g000019 Rmu_sc0006373.1_g000005 Rmu_sc0006514.1_g000028 Rmu_sc0006514.1_g000031 Rmu_sc0007367.1_g000010 Rmu_sc0011475.1_g000002 Rmu_sc0025304.1_g000001 Rmu_ssc0000161.1_g000030 Rmu_ssc0000161.1_g000032 Rmu_ssc0000387.1_g000018
rosa_roxburghii Rroxscaffold_1G00031090 Rroxscaffold_1G00032770 Rroxscaffold_1G00068950 Rroxscaffold_1G00069000 Rroxscaffold_1G00069010 Rroxscaffold_2G00129060 Rroxscaffold_2G00129100 Rroxscaffold_4G00309310 Rroxscaffold_4G00309320 Rroxscaffold_5G00350620 Rroxscaffold_5G00350630 Rroxscaffold_7G00162650 Rroxscaffold_7G00162660 Rroxscaffold_7G00162710
rosa_rugosa Rorug02G0564700 Rorug02G0564700 Rorug03G0343100 Rorug04G0189700 Rorug05G0102800 Rorug05G0102900 Rorug05G0103000 Rorug05G0106500 Rorug05G0106600 Rorug05G0106700 Rorug05G0252800 Rorug07G0182100 Rorug07G0283500 Rorug07G0283600
rosa_samantha Rh1AG004000 Rh1AG004100 Rh1AG004200 Rh1AG004400 Rh1AG004500 Rh1AG004600 Rh1AG004800 Rh1AG004900 Rh1AG005300 Rh1AG007000 Rh1AG115000 Rh1CG049400 Rh1CG055400 Rh1CG111000 Rh1DG053600 Rh1DG053700 Rh1DG059300 Rh1DG119700 Rh1DG177100 Rh1DG177200 Rh2AG353800 Rh2AG354000 Rh2AG354100 Rh2AG354300 Rh2AG354400 Rh2AG354500 Rh2AG354600 Rh2AG354700 Rh2AG642300 Rh2AG642400 Rh2AG642500 Rh2BG336500 Rh2BG336600 Rh2BG336700 Rh2BG336800 Rh2BG336900 Rh2CG299900 Rh2CG300000 Rh2CG300100 Rh2DG337000 Rh2DG337100 Rh3AG246600 Rh3BG121900 Rh3BG122300 Rh3BG122600 Rh3BG122700 Rh3DG304800 Rh3DG304900 Rh4AG148800 Rh4AG249000 Rh4AG249100 Rh4AG249200 Rh4BG182700 Rh4BG182800 Rh4BG182900 Rh4BG254400 Rh4BG254500 Rh4BG254600 Rh4CG155500 Rh4CG155700 Rh4DG057900 Rh4DG058000 Rh4DG058100 Rh4DG058200 Rh4DG129100 Rh5AG364500 Rh5AG364600 Rh5AG364800 Rh5AG365300 Rh5CG363700 Rh5CG497600 Rh5DG239500 Rh5DG239600 Rh6AG173500 Rh6AG175400 Rh6BG075900 Rh6BG076300 Rh6BG076400 Rh6BG076500 Rh6BG076600 Rh6CG151000 Rh6CG151200 Rh6CG151300 Rh6CG151400 Rh6CG205200 Rh6CG205300 Rh6CG419700 Rh6CG419900 Rh6CG420100 Rh6DG498700 Rh6DG498800 Rh7AG329000 Rh7AG329100 Rh7AG329300 Rh7AG329400 Rh7AG353000 Rh7AG353100 Rh7AG353300 Rh7AG353400 Rh7AG353500 Rh7CG346400 Rh7CG346500 Rh7CG346700 Rh7CG370800 Rh7CG370900 Rh7CG371000 Rh7CG371100 Rh7DG325000 Rh7DG325100 Rh7DG325200 Rh7DG325300 Rh7DG353100 Rh7DG353200 Rh7DG353300 Rh7DG363500 Rh7DG364000
rosa_wichuraiana Rw0G002290 Rw0G012780 Rw1G000260 Rw1G000270 Rw1G010400 Rw1G010410 Rw1G010420 Rw1G010430 Rw1G010440 Rw2G003210 Rw2G003220 Rw4G010970 Rw5G048520 Rw6G013320 Rw6G013470 Rw6G014940 Rw6G016570 Rw6G016590 Rw6G016600 Rw6G016610 Rw6G016620 Rw6G035450 Rw7G027760 Rw7G030460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 327, 1623
AccB1I GGYRCC 2 cut(s) 865, 1436
AccBSI CCGCTC 1 cut(s) 856
AccI GTMKAC 3 cut(s) 411, 1065, 1938
AccII CGCG 3 cut(s) 7, 100, 416
AclI AACGTT 1 cut(s) 1329
AclWI GGATC 4 cut(s) 768, 781, 1694, 2265
AcoI YGGCCR 1 cut(s) 1351
AcsI RAATTY 5 cut(s) 336, 422, 1130, 1634, 2137
AcuI CTGAAG 4 cut(s) 410, 575, 1408, 1616
AcvI CACGTG 1 cut(s) 431
AcyI GRCGYC 2 cut(s) 324, 1620
AfaI GTAC 5 cut(s) 660, 1347, 1589, 2124, 2159
AfiI CCNNNNNNNGG 7 cut(s) 282, 521, 1360, 1825, 1933, 2133, 2358
AflIII ACRYGT 5 cut(s) 428, 430, 1047, 1799, 2337
AgsI TTSAA 7 cut(s) 81, 235, 467, 1180, 1316, 2111, 2312
AhdI GACNNNNNGTC 2 cut(s) 1006, 1598
AhlI ACTAGT 1 cut(s) 977
AjiI CACGTC 3 cut(s) 130, 1069, 1475
AjnI CCWGG 3 cut(s) 166, 1978, 2290
AluBI AGCT 8 cut(s) 124, 512, 605, 811, 1141, 1422, 1778, 2300
AluI AGCT 8 cut(s) 124, 512, 605, 811, 1141, 1422, 1778, 2300
Alw21I GWGCWC 2 cut(s) 799, 1402
Alw26I GTCTC 5 cut(s) 143, 339, 944, 2095, 2181
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 4 cut(s) 768, 781, 1694, 2265
Ama87I CYCGRG 1 cut(s) 470
AoxI GGCC 4 cut(s) 273, 523, 1351, 1583
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 2 cut(s) 185, 1386
ApoI RAATTY 5 cut(s) 336, 422, 1130, 1634, 2137
Asp700I GAANNNNTTC 1 cut(s) 339
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 4 cut(s) 524, 1583, 1657, 1976
AsuC2I CCSGG 3 cut(s) 642, 779, 1582
AsuHPI GGTGA 9 cut(s) 34, 40, 488, 845, 959, 1195, 1496, 1729, 1825
AvaI CYCGRG 1 cut(s) 470
AvaII GGWCC 2 cut(s) 1657, 1976
BaeGI GKGCMC 1 cut(s) 799
BalI TGGCCA 1 cut(s) 1353
BamHI GGATCC 1 cut(s) 773
BanI GGYRCC 2 cut(s) 865, 1436
BbrPI CACGTG 1 cut(s) 431
BbsI GAAGAC 2 cut(s) 1050, 1551
Bbv12I GWGCWC 2 cut(s) 799, 1402
BbvI GCAGC 2 cut(s) 172, 1398
BccI CCATC 3 cut(s) 736, 1558, 1697
BceAI ACGGC 3 cut(s) 260, 1570, 2010
BcgI CGANNNNNNTGC 3 cut(s) 31, 1844, 1878
BciT130I CCWGG 3 cut(s) 168, 1980, 2292
BclI TGATCA 1 cut(s) 2326
BcnI CCSGG 3 cut(s) 642, 779, 1582
BcoDI GTCTC 5 cut(s) 143, 339, 944, 2095, 2181
BcuI ACTAGT 1 cut(s) 977
BfaI CTAG 7 cut(s) 114, 521, 629, 686, 812, 860, 978
BfmI CTRYAG 2 cut(s) 408, 806
BglI GCCNNNNNGGC 1 cut(s) 531
BisI GCNGC 3 cut(s) 186, 857, 1387
BlsI GCNGC 3 cut(s) 187, 858, 1388
Bme1390I CCNGG 6 cut(s) 168, 642, 779, 1582, 1980, 2292
Bme18I GGWCC 2 cut(s) 1657, 1976
BmeRI GACNNNNNGTC 2 cut(s) 1006, 1598
BmeT110I CYCGRG 1 cut(s) 470
BmgBI CACGTC 3 cut(s) 130, 1069, 1475
BmgT120I GGNCC 4 cut(s) 524, 1583, 1657, 1976
BmiI GGNNCC 6 cut(s) 45, 775, 867, 884, 1438, 1925
BmrFI CCNGG 6 cut(s) 168, 642, 779, 1582, 1980, 2292
BmrI ACTGGG 3 cut(s) 162, 521, 1819
BmsI GCATC 4 cut(s) 250, 507, 769, 2125
BmuI ACTGGG 3 cut(s) 162, 521, 1819
BoxI GACNNNNGTC 1 cut(s) 483
BpiI GAAGAC 2 cut(s) 1050, 1551
BpuEI CTTGAG 1 cut(s) 2253
BpuMI CCSGG 3 cut(s) 642, 779, 1582
BsaAI YACGTR 1 cut(s) 431
BsaHI GRCGYC 2 cut(s) 324, 1620
BsaJI CCNNGG 4 cut(s) 166, 777, 886, 2290
BsaWI WCCGGW 2 cut(s) 1115, 1394
Bsc4I CCNNNNNNNGG 7 cut(s) 282, 521, 1360, 1825, 1933, 2133, 2358
Bse1I ACTGG 5 cut(s) 157, 527, 1384, 1814, 1889
Bse3DI GCAATG 3 cut(s) 79, 311, 680
BseBI CCWGG 3 cut(s) 168, 1980, 2292
BseDI CCNNGG 4 cut(s) 166, 777, 886, 2290
BseGI GGATG 6 cut(s) 202, 797, 991, 1239, 2211, 2302
BseLI CCNNNNNNNGG 7 cut(s) 282, 521, 1360, 1825, 1933, 2133, 2358
BseMI GCAATG 3 cut(s) 79, 311, 680
BseMII CTCAG 1 cut(s) 614
BseNI ACTGG 5 cut(s) 157, 527, 1384, 1814, 1889
BseRI GAGGAG 2 cut(s) 1229, 2091
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 2 cut(s) 172, 1398
BseYI CCCAGC 1 cut(s) 907
Bsh1236I CGCG 3 cut(s) 7, 100, 416
Bsh1285I CGRYCG 1 cut(s) 1373
BshFI GGCC 4 cut(s) 275, 525, 1353, 1585
BshNI GGYRCC 2 cut(s) 865, 1436
BsiEI CGRYCG 1 cut(s) 1373
BsiHKAI GWGCWC 2 cut(s) 799, 1402
BsiHKCI CYCGRG 1 cut(s) 470
BsiSI CCGG 6 cut(s) 641, 779, 1116, 1395, 1581, 2352
BslFI GGGAC 6 cut(s) 314, 971, 988, 1581, 1742, 2002
BslI CCNNNNNNNGG 7 cut(s) 282, 521, 1360, 1825, 1933, 2133, 2358
BsmAI GTCTC 5 cut(s) 143, 339, 944, 2095, 2181
BsmBI CGTCTC 2 cut(s) 944, 2181
BsmFI GGGAC 6 cut(s) 314, 971, 988, 1581, 1742, 2002
BsmI GAATGC 1 cut(s) 441
BsnI GGCC 4 cut(s) 275, 525, 1353, 1585
BsoBI CYCGRG 1 cut(s) 470
Bsp1286I GDGCHC 2 cut(s) 799, 1402
Bsp1407I TGTACA 1 cut(s) 658
Bsp143I GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
BspANI GGCC 4 cut(s) 275, 525, 1353, 1585
BspCNI CTCAG 1 cut(s) 615
BspFNI CGCG 3 cut(s) 7, 100, 416
BspLI GGNNCC 6 cut(s) 45, 775, 867, 884, 1438, 1925
BspPI GGATC 4 cut(s) 768, 781, 1694, 2265
BspT107I GGYRCC 2 cut(s) 865, 1436
BsrBI CCGCTC 1 cut(s) 856
BsrDI GCAATG 3 cut(s) 79, 311, 680
BsrGI TGTACA 1 cut(s) 658
BsrI ACTGG 5 cut(s) 157, 527, 1384, 1814, 1889
BssECI CCNNGG 4 cut(s) 166, 777, 886, 2290
BssMI GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
BssNI GRCGYC 2 cut(s) 324, 1620
BssT1I CCWWGG 1 cut(s) 886
Bst2UI CCWGG 3 cut(s) 168, 1980, 2292
Bst4CI ACNGT 8 cut(s) 14, 244, 593, 2042, 2053, 2089, 2107, 2150
Bst6I CTCTTC 1 cut(s) 999
BstACI GRCGYC 2 cut(s) 324, 1620
BstAPI GCANNNNNTGC 1 cut(s) 725
BstAUI TGTACA 1 cut(s) 658
BstBAI YACGTR 1 cut(s) 431
BstC8I GCNNGC 3 cut(s) 70, 376, 459
BstDEI CTNAG 2 cut(s) 219, 623
BstEII GGTNACC 1 cut(s) 1735
BstF5I GGATG 6 cut(s) 202, 797, 991, 1239, 2211, 2302
BstFNI CGCG 3 cut(s) 7, 100, 416
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 5 cut(s) 776, 1057, 1689, 2260, 2329
BstMAI GTCTC 5 cut(s) 143, 339, 944, 2095, 2181
BstMBI GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
BstMCI CGRYCG 1 cut(s) 1373
BstMWI GCNNNNNNNGC 6 cut(s) 531, 725, 794, 853, 1228, 1290
BstNI CCWGG 3 cut(s) 168, 1980, 2292
BstNSI RCATGY 8 cut(s) 72, 378, 461, 1051, 1243, 1803, 2176, 2341
BstPAI GACNNNNGTC 1 cut(s) 483
BstPI GGTNACC 1 cut(s) 1735
BstSCI CCNGG 6 cut(s) 166, 640, 777, 1580, 1978, 2290
BstSFI CTRYAG 2 cut(s) 408, 806
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 3 cut(s) 7, 100, 416
BstV1I GCAGC 2 cut(s) 172, 1398
BstV2I GAAGAC 2 cut(s) 1050, 1551
BstX2I RGATCY 1 cut(s) 773
BstXI CCANNNNNNTGG 2 cut(s) 943, 1564
BstYI RGATCY 1 cut(s) 773
BsuRI GGCC 4 cut(s) 275, 525, 1353, 1585
BtgZI GCGATG 1 cut(s) 1274
BtrI CACGTC 3 cut(s) 130, 1069, 1475
BtsCI GGATG 6 cut(s) 202, 797, 991, 1239, 2211, 2302
BtsI GCAGTG 5 cut(s) 32, 180, 549, 735, 999
BtsIMutI CAGTG 7 cut(s) 32, 180, 549, 735, 999, 1377, 1625
Cac8I GCNNGC 3 cut(s) 70, 376, 459
CfoI GCGC 1 cut(s) 100
Cfr13I GGNCC 4 cut(s) 524, 1583, 1657, 1976
CseI GACGC 2 cut(s) 13, 422
Csp6I GTAC 5 cut(s) 659, 1346, 1588, 2123, 2158
CviQI GTAC 5 cut(s) 659, 1346, 1588, 2123, 2158
DdeI CTNAG 2 cut(s) 219, 623
DpnI GATC 5 cut(s) 775, 1056, 1688, 2259, 2328
DpnII GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
DriI GACNNNNNGTC 2 cut(s) 1006, 1598
EaeI YGGCCR 1 cut(s) 1351
Eam1104I CTCTTC 1 cut(s) 999
Eam1105I GACNNNNNGTC 2 cut(s) 1006, 1598
EarI CTCTTC 1 cut(s) 999
Eco130I CCWWGG 1 cut(s) 886
Eco47I GGWCC 2 cut(s) 1657, 1976
Eco57I CTGAAG 4 cut(s) 410, 575, 1408, 1616
Eco72I CACGTG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 470
Eco91I GGTNACC 1 cut(s) 1735
EcoO109I RGGNCCY 1 cut(s) 1657
EcoO65I GGTNACC 1 cut(s) 1735
EcoRI GAATTC 1 cut(s) 2137
EcoRII CCWGG 3 cut(s) 166, 1978, 2290
EcoT14I CCWWGG 1 cut(s) 886
EcoT22I ATGCAT 2 cut(s) 70, 311
ErhI CCWWGG 1 cut(s) 886
Esp3I CGTCTC 2 cut(s) 944, 2181
FaqI GGGAC 6 cut(s) 314, 971, 988, 1581, 1742, 2002
FauI CCCGC 4 cut(s) 181, 701, 781, 1990
FauNDI CATATG 1 cut(s) 2143
FbaI TGATCA 1 cut(s) 2326
FblI GTMKAC 3 cut(s) 411, 1065, 1938
Fnu4HI GCNGC 3 cut(s) 186, 857, 1387
FokI GGATG 6 cut(s) 209, 804, 998, 1246, 2218, 2289
Fsp4HI GCNGC 3 cut(s) 186, 857, 1387
FspBI CTAG 7 cut(s) 114, 521, 629, 686, 812, 860, 978
GlaI GCGC 1 cut(s) 99
GluI GCNGC 3 cut(s) 186, 857, 1387
GsaI CCCAGC 1 cut(s) 911
HaeIII GGCC 4 cut(s) 275, 525, 1353, 1585
HapII CCGG 6 cut(s) 641, 779, 1116, 1395, 1581, 2352
HgaI GACGC 2 cut(s) 13, 422
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 2 cut(s) 324, 1620
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 3 cut(s) 586, 1066, 2211
HindII GTYRAC 3 cut(s) 586, 1066, 2211
HindIII AAGCTT 2 cut(s) 122, 1420
HinfI GANTC 4 cut(s) 1007, 1169, 1224, 2266
HpaII CCGG 6 cut(s) 641, 779, 1116, 1395, 1581, 2352
HphI GGTGA 9 cut(s) 34, 40, 488, 845, 959, 1195, 1496, 1729, 1825
Hpy188III TCNNGA 4 cut(s) 344, 767, 1660, 1764
Hpy99I CGWCG 5 cut(s) 329, 1070, 1622, 1625, 1856
HpyCH4III ACNGT 8 cut(s) 14, 244, 593, 2042, 2053, 2089, 2107, 2150
HpyCH4IV ACGT 8 cut(s) 129, 324, 430, 1068, 1329, 1474, 1620, 2160
HpyF10VI GCNNNNNNNGC 6 cut(s) 531, 725, 794, 853, 1228, 1290
HpyF3I CTNAG 2 cut(s) 219, 623
HpySE526I ACGT 8 cut(s) 129, 324, 430, 1068, 1329, 1474, 1620, 2160
Hsp92I GRCGYC 2 cut(s) 324, 1620
HspAI GCGC 1 cut(s) 98
Ksp22I TGATCA 1 cut(s) 2326
Kzo9I GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
LmnI GCTCC 8 cut(s) 517, 704, 853, 882, 1138, 1252, 1397, 2281
Lsp1109I GCAGC 2 cut(s) 172, 1398
LweI GCATC 4 cut(s) 250, 507, 769, 2125
MaeI CTAG 7 cut(s) 114, 521, 629, 686, 812, 860, 978
MaeII ACGT 8 cut(s) 129, 324, 430, 1068, 1329, 1474, 1620, 2160
MalI GATC 5 cut(s) 775, 1056, 1688, 2259, 2328
MbiI CCGCTC 1 cut(s) 856
MboI GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
MfeI CAATTG 1 cut(s) 961
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 2 cut(s) 799, 1402
MlsI TGGCCA 1 cut(s) 1353
MluNI TGGCCA 1 cut(s) 1353
MlyI GAGTC 2 cut(s) 1016, 2260
MmeI TCCRAC 3 cut(s) 1353, 1388, 1821
Mox20I TGGCCA 1 cut(s) 1353
Mph1103I ATGCAT 2 cut(s) 70, 311
MroXI GAANNNNTTC 1 cut(s) 339
MscI TGGCCA 1 cut(s) 1353
MseI TTAA 7 cut(s) 63, 270, 1025, 1079, 1443, 2009, 2063
MslI CAYNNNNRTG 5 cut(s) 1232, 1293, 1353, 1473, 1911
Msp20I TGGCCA 1 cut(s) 1353
MspA1I CMGCKG 1 cut(s) 1126
MspI CCGG 6 cut(s) 641, 779, 1116, 1395, 1581, 2352
MspR9I CCNGG 6 cut(s) 168, 642, 779, 1582, 1980, 2292
MunI CAATTG 1 cut(s) 961
Mva1269I GAATGC 1 cut(s) 441
MvaI CCWGG 3 cut(s) 168, 1980, 2292
MvnI CGCG 3 cut(s) 7, 100, 416
MwoI GCNNNNNNNGC 6 cut(s) 531, 725, 794, 853, 1228, 1290
NciI CCSGG 3 cut(s) 642, 779, 1582
NdeI CATATG 1 cut(s) 2143
NdeII GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
NlaIV GGNNCC 6 cut(s) 45, 775, 867, 884, 1438, 1925
NmuCI GTSAC 7 cut(s) 28, 476, 569, 821, 1600, 1735, 2101
NsiI ATGCAT 2 cut(s) 70, 311
NspI RCATGY 8 cut(s) 72, 378, 461, 1051, 1243, 1803, 2176, 2341
PaeI GCATGC 3 cut(s) 72, 378, 461
PciI ACATGT 3 cut(s) 1047, 1799, 2337
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PctI GAATGC 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 1169, 1224
PflFI GACNNNGTC 4 cut(s) 326, 350, 1733, 1943
PfoI TCCNGGA 1 cut(s) 640
PkrI GCNGC 3 cut(s) 187, 858, 1388
PleI GAGTC 2 cut(s) 1015, 2260
PmaCI CACGTG 1 cut(s) 431
PmlI CACGTG 1 cut(s) 431
PpsI GAGTC 2 cut(s) 1015, 2260
Ppu21I YACGTR 1 cut(s) 431
PpuMI RGGWCCY 1 cut(s) 1657
PscI ACATGT 3 cut(s) 1047, 1799, 2337
PshAI GACNNNNGTC 1 cut(s) 483
Psp1406I AACGTT 1 cut(s) 1329
Psp5II RGGWCCY 1 cut(s) 1657
Psp6I CCWGG 3 cut(s) 166, 1978, 2290
PspCI CACGTG 1 cut(s) 431
PspEI GGTNACC 1 cut(s) 1735
PspFI CCCAGC 1 cut(s) 907
PspGI CCWGG 3 cut(s) 166, 1978, 2290
PspN4I GGNNCC 6 cut(s) 45, 775, 867, 884, 1438, 1925
PspPI GGNCC 4 cut(s) 524, 1583, 1657, 1976
PspPPI RGGWCCY 1 cut(s) 1657
PsuI RGATCY 1 cut(s) 773
PsyI GACNNNGTC 4 cut(s) 326, 350, 1733, 1943
RsaI GTAC 5 cut(s) 660, 1347, 1589, 2124, 2159
RsaNI GTAC 5 cut(s) 659, 1346, 1588, 2123, 2158
RseI CAYNNNNRTG 5 cut(s) 1232, 1293, 1353, 1473, 1911
SalI GTCGAC 1 cut(s) 1064
SaqAI TTAA 7 cut(s) 63, 270, 1025, 1079, 1443, 2009, 2063
SatI GCNGC 3 cut(s) 186, 857, 1387
Sau3AI GATC 5 cut(s) 773, 1054, 1686, 2257, 2326
Sau96I GGNCC 4 cut(s) 524, 1583, 1657, 1976
SchI GAGTC 2 cut(s) 1016, 2260
ScrFI CCNGG 6 cut(s) 168, 642, 779, 1582, 1980, 2292
SduI GDGCHC 2 cut(s) 799, 1402
SfaNI GCATC 4 cut(s) 250, 507, 769, 2125
SfcI CTRYAG 2 cut(s) 408, 806
SinI GGWCC 2 cut(s) 1657, 1976
SmiMI CAYNNNNRTG 5 cut(s) 1232, 1293, 1353, 1473, 1911
SmlI CTYRAG 1 cut(s) 2268
SmoI CTYRAG 1 cut(s) 2268
SpeI ACTAGT 1 cut(s) 977
SphI GCATGC 3 cut(s) 72, 378, 461
SspMI CTAG 7 cut(s) 114, 521, 629, 686, 812, 860, 978
StyD4I CCNGG 6 cut(s) 166, 640, 777, 1580, 1978, 2290
StyI CCWWGG 1 cut(s) 886
TaaI ACNGT 8 cut(s) 14, 244, 593, 2042, 2053, 2089, 2107, 2150
TaiI ACGT 8 cut(s) 132, 327, 433, 1071, 1332, 1477, 1623, 2163
TaqI TCGA 8 cut(s) 343, 363, 1065, 1562, 1617, 1806, 1851, 2260
TaqII GACCGA 1 cut(s) 1359
TatI WGTACW 2 cut(s) 658, 1345
TauI GCSGC 1 cut(s) 859
TfiI GAWTC 2 cut(s) 1169, 1224
Tru1I TTAA 7 cut(s) 63, 270, 1025, 1079, 1443, 2009, 2063
Tru9I TTAA 7 cut(s) 63, 270, 1025, 1079, 1443, 2009, 2063
TscAI CASTG 7 cut(s) 39, 187, 549, 735, 999, 1384, 1632
TseFI GTSAC 7 cut(s) 28, 476, 569, 821, 1600, 1735, 2101
TseI GCWGC 2 cut(s) 185, 1386
Tsp45I GTSAC 7 cut(s) 28, 476, 569, 821, 1600, 1735, 2101
TspGWI ACGGA 2 cut(s) 383, 1265
TspRI CASTG 7 cut(s) 39, 187, 549, 735, 999, 1384, 1632
Tth111I GACNNNGTC 4 cut(s) 326, 350, 1733, 1943
VneI GTGCAC 1 cut(s) 795
VpaK11BI GGWCC 2 cut(s) 1657, 1976
XapI RAATTY 5 cut(s) 336, 422, 1130, 1634, 2137
XceI RCATGY 8 cut(s) 72, 378, 461, 1051, 1243, 1803, 2176, 2341
XcmI CCANNNNNNNNNTGG 1 cut(s) 2313
XmiI GTMKAC 3 cut(s) 411, 1065, 1938
XmnI GAANNNNTTC 1 cut(s) 339
XspI CTAG 7 cut(s) 114, 521, 629, 686, 812, 860, 978
ZraI GACGTC 2 cut(s) 325, 1621
Zsp2I ATGCAT 2 cut(s) 70, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.