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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 91, 257 |
| AcoI | YGGCCR | 1 cut(s) | 202 |
| AfaI | GTAC | 1 cut(s) | 297 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 32 |
| AgsI | TTSAA | 1 cut(s) | 113 |
| AluBI | AGCT | 2 cut(s) | 16, 49 |
| AluI | AGCT | 2 cut(s) | 16, 49 |
| Alw26I | GTCTC | 1 cut(s) | 166 |
| AoxI | GGCC | 1 cut(s) | 202 |
| ApeKI | GCWGC | 1 cut(s) | 13 |
| AspS9I | GGNCC | 2 cut(s) | 35, 226 |
| AsuC2I | CCSGG | 1 cut(s) | 206 |
| AsuHPI | GGTGA | 1 cut(s) | 56 |
| AvaII | GGWCC | 2 cut(s) | 35, 226 |
| BbvI | GCAGC | 1 cut(s) | 25 |
| BccI | CCATC | 1 cut(s) | 72 |
| BcnI | CCSGG | 1 cut(s) | 206 |
| BcoDI | GTCTC | 1 cut(s) | 166 |
| BisI | GCNGC | 1 cut(s) | 14 |
| BlsI | GCNGC | 1 cut(s) | 15 |
| Bme1390I | CCNGG | 1 cut(s) | 206 |
| Bme18I | GGWCC | 2 cut(s) | 35, 226 |
| BmgT120I | GGNCC | 2 cut(s) | 35, 226 |
| BmiI | GGNNCC | 2 cut(s) | 36, 228 |
| BmrFI | CCNGG | 1 cut(s) | 206 |
| BmsI | GCATC | 2 cut(s) | 75, 268 |
| BpuMI | CCSGG | 1 cut(s) | 206 |
| BsaI | GGTCTC | 1 cut(s) | 166 |
| BsaWI | WCCGGW | 1 cut(s) | 25 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 32 |
| Bse118I | RCCGGY | 1 cut(s) | 292 |
| Bse1I | ACTGG | 2 cut(s) | 79, 176 |
| BseGI | GGATG | 2 cut(s) | 83, 283 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 32 |
| BseNI | ACTGG | 2 cut(s) | 79, 176 |
| BseXI | GCAGC | 1 cut(s) | 25 |
| BsgI | GTGCAG | 1 cut(s) | 209 |
| BshFI | GGCC | 1 cut(s) | 204 |
| BsiSI | CCGG | 3 cut(s) | 26, 205, 293 |
| BslFI | GGGAC | 2 cut(s) | 48, 212 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 32 |
| BsmAI | GTCTC | 1 cut(s) | 166 |
| BsmFI | GGGAC | 2 cut(s) | 48, 212 |
| BsnI | GGCC | 1 cut(s) | 204 |
| Bso31I | GGTCTC | 1 cut(s) | 166 |
| BspACI | CCGC | 2 cut(s) | 91, 257 |
| BspANI | GGCC | 1 cut(s) | 204 |
| BspLI | GGNNCC | 2 cut(s) | 36, 228 |
| BspTNI | GGTCTC | 1 cut(s) | 166 |
| BsrFI | RCCGGY | 1 cut(s) | 292 |
| BsrI | ACTGG | 2 cut(s) | 79, 176 |
| BssAI | RCCGGY | 1 cut(s) | 292 |
| BstC8I | GCNNGC | 1 cut(s) | 259 |
| BstF5I | GGATG | 2 cut(s) | 83, 283 |
| BstMAI | GTCTC | 1 cut(s) | 166 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 134, 143 |
| BstSCI | CCNGG | 1 cut(s) | 204 |
| BstV1I | GCAGC | 1 cut(s) | 25 |
| BsuRI | GGCC | 1 cut(s) | 204 |
| BtsCI | GGATG | 2 cut(s) | 83, 283 |
| BtsIMutI | CAGTG | 2 cut(s) | 72, 183 |
| Cac8I | GCNNGC | 1 cut(s) | 259 |
| Cfr10I | RCCGGY | 1 cut(s) | 292 |
| Cfr13I | GGNCC | 2 cut(s) | 35, 226 |
| Csp6I | GTAC | 1 cut(s) | 296 |
| CviAII | CATG | 3 cut(s) | 134, 245, 317 |
| CviJI | RGCY | 7 cut(s) | 16, 49, 83, 128, 153, 204, 261 |
| CviKI_1 | RGCY | 7 cut(s) | 16, 49, 83, 128, 153, 204, 261 |
| CviQI | GTAC | 1 cut(s) | 296 |
| EaeI | YGGCCR | 1 cut(s) | 202 |
| Eco31I | GGTCTC | 1 cut(s) | 166 |
| Eco47I | GGWCC | 2 cut(s) | 35, 226 |
| FaeI | CATG | 3 cut(s) | 137, 248, 320 |
| FaiI | YATR | 7 cut(s) | 32, 40, 42, 96, 135, 246, 318 |
| FaqI | GGGAC | 2 cut(s) | 48, 212 |
| FatI | CATG | 3 cut(s) | 133, 244, 316 |
| FauI | CCCGC | 1 cut(s) | 250 |
| FauNDI | CATATG | 1 cut(s) | 40 |
| Fnu4HI | GCNGC | 1 cut(s) | 14 |
| FokI | GGATG | 2 cut(s) | 90, 290 |
| Fsp4HI | GCNGC | 1 cut(s) | 14 |
| GluI | GCNGC | 1 cut(s) | 14 |
| HaeIII | GGCC | 1 cut(s) | 204 |
| HapII | CCGG | 3 cut(s) | 26, 205, 293 |
| Hin1II | CATG | 3 cut(s) | 137, 248, 320 |
| HinfI | GANTC | 3 cut(s) | 87, 109, 216 |
| HpaII | CCGG | 3 cut(s) | 26, 205, 293 |
| HphI | GGTGA | 1 cut(s) | 56 |
| Hpy188I | TCNGA | 3 cut(s) | 22, 287, 311 |
| HpyAV | CCTTC | 1 cut(s) | 268 |
| HpyCH4V | TGCA | 1 cut(s) | 190 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 134, 143 |
| Hsp92II | CATG | 3 cut(s) | 137, 248, 320 |
| LpnPI | CCDG | 6 cut(s) | 39, 60, 69, 189, 218, 306 |
| Lsp1109I | GCAGC | 1 cut(s) | 25 |
| LweI | GCATC | 2 cut(s) | 75, 268 |
| MmeI | TCCRAC | 1 cut(s) | 334 |
| MnlI | CCTC | 1 cut(s) | 63 |
| MseI | TTAA | 2 cut(s) | 183, 324 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| MspI | CCGG | 3 cut(s) | 26, 205, 293 |
| MspR9I | CCNGG | 1 cut(s) | 206 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 134, 143 |
| NciI | CCSGG | 1 cut(s) | 206 |
| NdeI | CATATG | 1 cut(s) | 40 |
| NlaIII | CATG | 3 cut(s) | 137, 248, 320 |
| NlaIV | GGNNCC | 2 cut(s) | 36, 228 |
| PfeI | GAWTC | 3 cut(s) | 87, 109, 216 |
| PkrI | GCNGC | 1 cut(s) | 15 |
| PspN4I | GGNNCC | 2 cut(s) | 36, 228 |
| PspPI | GGNCC | 2 cut(s) | 35, 226 |
| RsaI | GTAC | 1 cut(s) | 297 |
| RsaNI | GTAC | 1 cut(s) | 296 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SaqAI | TTAA | 2 cut(s) | 183, 324 |
| SatI | GCNGC | 1 cut(s) | 14 |
| Sau96I | GGNCC | 2 cut(s) | 35, 226 |
| ScrFI | CCNGG | 1 cut(s) | 206 |
| SetI | ASST | 4 cut(s) | 18, 51, 189, 225 |
| SfaNI | GCATC | 2 cut(s) | 75, 268 |
| SinI | GGWCC | 2 cut(s) | 35, 226 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SsiI | CCGC | 2 cut(s) | 91, 257 |
| StyD4I | CCNGG | 1 cut(s) | 204 |
| TaqI | TCGA | 2 cut(s) | 69, 345 |
| TfiI | GAWTC | 3 cut(s) | 87, 109, 216 |
| Tru1I | TTAA | 2 cut(s) | 183, 324 |
| Tru9I | TTAA | 2 cut(s) | 183, 324 |
| TscAI | CASTG | 2 cut(s) | 79, 183 |
| TseI | GCWGC | 1 cut(s) | 13 |
| TspDTI | ATGAA | 2 cut(s) | 287, 305 |
| TspRI | CASTG | 2 cut(s) | 79, 183 |
| VpaK11BI | GGWCC | 2 cut(s) | 35, 226 |