Rh5AG365300

protein desumoylation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000124
Physical Location & Seq
Forward (+)
61636665 .. 61640175
3511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG365300.1

Sequence Viewer

Length: 2553 bp
ATGAAGGCCCGGCGAGGTAATCTATTCGGTTCTCCACCAAATATCACGGGAGCCACTGGAGCAAATGACTCACCCAATACCCGTGATGTGGCTACGAGATCACCTGAACTTGTTATGGGGGACTTGGTGACAAATTCCAAACCCTATGAGTTCACACTCACCGTATCTGGACTCTCCGTTGAAAAGGTGGCTGCCATAGCTGAGATGGGCTTTGATGGTCTCTTGCAGATTGGATGCAACACTCTTGTTGGACCTGTCTGGAGGCTGATGGTGCACAGTGTTGACCTCGGCAACCGCAGAGTGTCGATAATGGGCCAGAACTTTGCAGTTACTCCACATGAGTTTGGCCGTGTAATGGGAGTGCAGGATGGAGGCTCTGACTTTGAACTGGGGACTGCCACAGACGTTGCTGAGTATGGCATGATTGTTGAAGACATGTGTGACAGAGAAGGAAGGATCCAGTACTGTCATCTGAAGGAAATAGTACGGGACCGTGAGGAAGCCGACGACAAGTTCAGGATCGCATTTTCGTTGCTTGCACTAGGTTTGGTTCTGTGCCCTACAACAGATGACGCTGTTGACACGTCTCTGATTCTACCCCTTCTGGAAACGGGACGCATTGGGCAGAAGAACTGGGCTTCCTTTTGTCTCCAAAGATTGCTAGACTCTGTGACGGCCCAGAGGGAACATGGGGACGGTGTTGTTGGGGGGTGTGTGCTCTTCCTCCAGGTTCTATACTTCGAGAAGGTCGGCTCGGCTTGTTTTCATATTGATAGGTCTATACTTCCACTTATGGCCTGGGGGGACTTTGAGGTAAGGGAGTTGATAACCAGGGTTAGGACATATGGTGGCTTTAAGAGTCCAATTGTCATGATGGAGAGGAACAAGCGGGTGAAAATGGCTAGTAAATGCGCCGATGATGATGGTGAGTTTGGACTCGGACTGAGGAAGTCAGTTCATGAGGACATCTTCGACGTCAAGTTGGAGCTCATGGGGGTCAAGGGTGACGTGTGGAGGCTCATGGATGCTTGTGGGAGGATTCAGCCTGATGTGGCCGAACTAGCAAGGAAAGTGGAGATTATAGCACATCAGATAAGCGGTTTGAGGGAGGAATGTGCCGACATGGTGAAGATCCAACTGCTGAGATCGGCAAACAGGAGGGGGGATGACTGCAACGTTCACACAGTCCCTGTGTGGAAACCGCGGCAGAGGGATGGAATGGGGAGTGGAGATTCAGTCCTCCATGCTGACACATTTCTGGAGGAAGAGAGCTTGCAGCGAATGAAGGCAAAATCAAAGCTATGTGATAATGCTTTTGGTAAGGCAGTATCTGCCAGCTCAAGCAGTCCTGGTGCCGCCCCAACAAGAGAGATGAGACCATATCAGGGCGGTTGTGTGCCTGAATCCAAGGCAGTTGTATCTCCACATGCCAGGGCCATGTTCACTTATGCAGTCACCCGCAACGAGGAGGTCAATCATGTGATTAGTTCGGGCTGTAATGGAGCAGGGCTCACTAATGCCAAAAGTTGGATGCTGCCAGGACCTTACATGCTGATGCACCCTATAGGGAATGACGACATTCTCCTAATCAATTACATTTTCATGGGTGGGAAGAAAGTTACGGTGGAGGAGGGAAGAACTATGGTTGCTTCAACCGGGAATCAGAAGGCACTACTTTACCGTGATGAGGCCTTATGCTTGGGGCCAGCCACAAATGTTTCCAGCTTTGTCATCAACTTTGTCGCAGAATGCTTGAACAAGGATGGGAGTGGGGACTGGTTTTTTCCTACTTACTTCTCGGAAAAGGCCAGTTCGTACAATGCAGGGGAATCGTTTCGGGAATGGGTGGAGCACACAAGGACACTCTGCAACCTTGATAGATTCAACGGTTGCATGAAGAATTGCAAGAGGATGTTTTTGCCGATGCACGAGGACAAGGCCGGGGGACATTGGTTTCTGATGGTGGTTGATCTAGAAAAGAAGGTGGCTGAGGTTTGGGACAGTCTGCCGACTCTGTCATCAGCAAGCAGTAGATGGGACCATGCACTGACAACGCTGCGCCATCTTGACCATTGCTTTTACTTTGACAAGTTGAGGATGGAAGGGACCAACACTGACTTTGGGTTATTCAACTATGTTGAGCCTGTAGATGCACCTAAACAGGCAAATGGTTTCGACTGTGGGGTTTATATCATCCTTAATATGATGCACTACCGGACCAATTGGCTGAAAGGGGCCGGGTCAGATGATCTTAGAGCAAGGGTGCTTCTTCGGTGTCTCAAAGCCCCTGATAATGAGAGGGCTACGGTTACACTAAGAGAAGCACACTCCTTTGCTGTTGATAAGGGATCTGAGGGAGGTATGAAGGAGGACAAGATAGTGGAGCCAAATGGATGGGAAGGTATTGATCTGTCTGAGTTTGATGAACTGGATGATGATTTGGGAGTTACTCAAGTTGGTGATCCGAGTACAGCGCCAATTGGTGAGTCAAGCATATTGAAGAGGAACATGGACCTTAGGGAAGGCTTTCCTAAGGGAATGTTCCGTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48

Protein Analysis

850

Amino Acids

94.23

Weight (kDa)

5.53

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 610 - 737 6.4e-13 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000124)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10992 FvH4_2g25150 FvH4_2g27910 FvH4_2g27931 FvH4_4g09443 FvH4_4g09490 FvH4_4g15990 FvH4_4g15991 FvH4_6g07180 FvH4_6g40320 FvH4_7g10222 FvH4_7g10251
rosa_chinensis RchiOBHm_Chr2g0155611
rosa_laevigata RLG00000009253 RLG00000009254 RLG00000011848 RLG00000017301 RLG00000017302 RLG00000034909
rosa_multiflora Rmu_co8063658.1_g000001 Rmu_co8240443.1_g000001 Rmu_co8264451.1_g000001 Rmu_co8381937.1_g000001 Rmu_co8419903.1_g000001 Rmu_co8513405.1_g000002 Rmu_co8522915.1_g000001 Rmu_co8522915.1_g000004 Rmu_sc0000279.1_g000024 Rmu_sc0000279.1_g000048 Rmu_sc0000642.1_g000017 Rmu_sc0002045.1_g000015 Rmu_sc0002045.1_g000018 Rmu_sc0002045.1_g000023 Rmu_sc0002045.1_g000028 Rmu_sc0003047.1_g000008 Rmu_sc0003179.1_g000019 Rmu_sc0003179.1_g000020 Rmu_sc0003179.1_g000022 Rmu_sc0003179.1_g000023 Rmu_sc0003422.1_g000011 Rmu_sc0004999.1_g000014 Rmu_sc0005712.1_g000018 Rmu_sc0005712.1_g000023 Rmu_sc0006198.1_g000019 Rmu_sc0006373.1_g000005 Rmu_sc0006514.1_g000028 Rmu_sc0006514.1_g000031 Rmu_sc0007367.1_g000010 Rmu_sc0011475.1_g000002 Rmu_sc0025304.1_g000001 Rmu_ssc0000161.1_g000030 Rmu_ssc0000161.1_g000032 Rmu_ssc0000387.1_g000018
rosa_roxburghii Rroxscaffold_1G00031090 Rroxscaffold_1G00032770 Rroxscaffold_1G00068950 Rroxscaffold_1G00069000 Rroxscaffold_1G00069010 Rroxscaffold_2G00129060 Rroxscaffold_2G00129100 Rroxscaffold_4G00309310 Rroxscaffold_4G00309320 Rroxscaffold_5G00350620 Rroxscaffold_5G00350630 Rroxscaffold_7G00162650 Rroxscaffold_7G00162660 Rroxscaffold_7G00162710
rosa_rugosa Rorug02G0564700 Rorug02G0564700 Rorug03G0343100 Rorug04G0189700 Rorug05G0102800 Rorug05G0102900 Rorug05G0103000 Rorug05G0106500 Rorug05G0106600 Rorug05G0106700 Rorug05G0252800 Rorug07G0182100 Rorug07G0283500 Rorug07G0283600
rosa_samantha Rh1AG004000 Rh1AG004100 Rh1AG004200 Rh1AG004400 Rh1AG004500 Rh1AG004600 Rh1AG004800 Rh1AG004900 Rh1AG005300 Rh1AG007000 Rh1AG115000 Rh1CG049400 Rh1CG055400 Rh1CG111000 Rh1DG053600 Rh1DG053700 Rh1DG059300 Rh1DG119700 Rh1DG177100 Rh1DG177200 Rh2AG353800 Rh2AG354000 Rh2AG354100 Rh2AG354300 Rh2AG354400 Rh2AG354500 Rh2AG354600 Rh2AG354700 Rh2AG642300 Rh2AG642400 Rh2AG642500 Rh2BG336500 Rh2BG336600 Rh2BG336700 Rh2BG336800 Rh2BG336900 Rh2CG299900 Rh2CG300000 Rh2CG300100 Rh2DG337000 Rh2DG337100 Rh3AG246600 Rh3BG121900 Rh3BG122300 Rh3BG122600 Rh3BG122700 Rh3DG304800 Rh3DG304900 Rh4AG148800 Rh4AG249000 Rh4AG249100 Rh4AG249200 Rh4BG182700 Rh4BG182800 Rh4BG182900 Rh4BG254400 Rh4BG254500 Rh4BG254600 Rh4CG155500 Rh4CG155700 Rh4DG057900 Rh4DG058000 Rh4DG058100 Rh4DG058200 Rh4DG129100 Rh5AG364500 Rh5AG364600 Rh5AG364800 Rh5AG365300 Rh5CG363700 Rh5CG497600 Rh5DG239500 Rh5DG239600 Rh6AG173500 Rh6AG175400 Rh6BG075900 Rh6BG076300 Rh6BG076400 Rh6BG076500 Rh6BG076600 Rh6CG151000 Rh6CG151200 Rh6CG151300 Rh6CG151400 Rh6CG205200 Rh6CG205300 Rh6CG419700 Rh6CG419900 Rh6CG420100 Rh6DG498700 Rh6DG498800 Rh7AG329000 Rh7AG329100 Rh7AG329300 Rh7AG329400 Rh7AG353000 Rh7AG353100 Rh7AG353300 Rh7AG353400 Rh7AG353500 Rh7CG346400 Rh7CG346500 Rh7CG346700 Rh7CG370800 Rh7CG370900 Rh7CG371000 Rh7CG371100 Rh7DG325000 Rh7DG325100 Rh7DG325200 Rh7DG325300 Rh7DG353100 Rh7DG353200 Rh7DG353300 Rh7DG363500 Rh7DG364000
rosa_wichuraiana Rw0G002290 Rw0G012780 Rw1G000260 Rw1G000270 Rw1G010400 Rw1G010410 Rw1G010420 Rw1G010430 Rw1G010440 Rw2G003210 Rw2G003220 Rw4G010970 Rw5G048520 Rw6G013320 Rw6G013470 Rw6G014940 Rw6G016570 Rw6G016590 Rw6G016600 Rw6G016610 Rw6G016620 Rw6G035450 Rw7G027760 Rw7G030460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 978
AccB1I GGYRCC 1 cut(s) 1352
AccB7I CCANNNNNTGG 1 cut(s) 1527
AccII CGCG 1 cut(s) 1204
AciI CCGC 8 cut(s) 295, 889, 1098, 1202, 1204, 1356, 1389, 1459
AclI AACGTT 1 cut(s) 1176
AclWI GGATC 6 cut(s) 451, 464, 527, 1126, 2356, 2456
AcoI YGGCCR 2 cut(s) 346, 1053
AcsI RAATTY 1 cut(s) 133
AcuI CTGAAG 1 cut(s) 494
AcyI GRCGYC 1 cut(s) 975
AfaI GTAC 4 cut(s) 464, 486, 1817, 2470
AfiI CCNNNNNNNGG 7 cut(s) 88, 355, 837, 1385, 1465, 1527, 1687
AflIII ACRYGT 3 cut(s) 435, 582, 1008
AgsI TTSAA 8 cut(s) 182, 386, 431, 1653, 1756, 1885, 2131, 2500
AjiI CACGTC 2 cut(s) 585, 1009
AjnI CCWGG 6 cut(s) 726, 797, 830, 1348, 1430, 1537
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 6 cut(s) 200, 988, 1272, 1300, 1338, 1725
AluI AGCT 6 cut(s) 200, 988, 1272, 1300, 1338, 1725
Alw21I GWGCWC 4 cut(s) 276, 720, 990, 1854
Alw26I GTCTC 5 cut(s) 224, 591, 653, 1369, 2282
Alw44I GTGCAC 1 cut(s) 272
AlwI GGATC 6 cut(s) 451, 464, 527, 1126, 2356, 2456
AlwNI CAGNNNCTG 2 cut(s) 1190, 1331
ApaLI GTGCAC 1 cut(s) 272
ApeKI GCWGC 4 cut(s) 191, 1276, 1534, 2056
ApoI RAATTY 1 cut(s) 133
Asp700I GAANNNNTTC 2 cut(s) 1833, 2526
AspLEI GCGC 3 cut(s) 914, 2061, 2476
AsuC2I CCSGG 4 cut(s) 10, 1657, 1942, 2239
AvaII GGWCC 7 cut(s) 251, 490, 1541, 2038, 2106, 2217, 2512
AxyI CCTNAGG 2 cut(s) 2516, 2532
BaeGI GKGCMC 2 cut(s) 276, 560
BamHI GGATCC 1 cut(s) 456
BanI GGYRCC 1 cut(s) 1352
BanII GRGCYC 2 cut(s) 990, 1512
BauI CACGAG 1 cut(s) 1928
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 4 cut(s) 276, 720, 990, 1854
BbvCI CCTCAGC 1 cut(s) 1989
BbvI GCAGC 4 cut(s) 178, 1288, 1521, 2043
BceAI ACGGC 2 cut(s) 333, 690
BcgI CGANNNNNNTGC 2 cut(s) 1812, 1846
BciT130I CCWGG 6 cut(s) 728, 799, 832, 1350, 1432, 1539
BcnI CCSGG 4 cut(s) 10, 1657, 1942, 2239
BcoDI GTCTC 5 cut(s) 224, 591, 653, 1369, 2282
BfaI CTAG 5 cut(s) 542, 662, 903, 1061, 1973
BfmI CTRYAG 2 cut(s) 1563, 2145
BfoI RGCGCY 1 cut(s) 2477
BisI GCNGC 6 cut(s) 192, 1205, 1277, 1356, 1535, 2057
BlsI GCNGC 6 cut(s) 193, 1206, 1278, 1357, 1536, 2058
BmcAI AGTACT 1 cut(s) 464
Bme18I GGWCC 7 cut(s) 251, 490, 1541, 2038, 2106, 2217, 2512
BmgBI CACGTC 2 cut(s) 585, 1009
BmiI GGNNCC 9 cut(s) 52, 458, 491, 1354, 1704, 2039, 2107, 2236, 2385
BmrI ACTGGG 2 cut(s) 398, 643
BmsI GCATC 7 cut(s) 224, 1015, 1521, 1545, 1914, 2140, 2196
BmuI ACTGGG 2 cut(s) 398, 643
BpiI GAAGAC 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 1494, 1526
BpmI CTGGAG 4 cut(s) 78, 280, 710, 1280
Bpu10I CCTNAGC 1 cut(s) 1989
BpuEI CTTGAG 2 cut(s) 1324, 2436
BpuMI CCSGG 4 cut(s) 10, 1657, 1942, 2239
BsaHI GRCGYC 1 cut(s) 975
BsaI GGTCTC 2 cut(s) 224, 1369
BsaJI CCNNGG 7 cut(s) 286, 798, 831, 1202, 1407, 1431, 1941
BsaWI WCCGGW 1 cut(s) 2214
BsaXI ACNNNNNCTCC 2 cut(s) 1221, 1251
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 355, 837, 1385, 1465, 1527, 1687
Bse1I ACTGG 7 cut(s) 61, 393, 460, 638, 1781, 1809, 2433
Bse21I CCTNAGG 2 cut(s) 2516, 2532
Bse3DI GCAATG 1 cut(s) 2071
BseBI CCWGG 6 cut(s) 728, 799, 832, 1350, 1432, 1539
BseDI CCNNGG 7 cut(s) 286, 798, 831, 1202, 1407, 1431, 1941
BseLI CCNNNNNNNGG 7 cut(s) 88, 355, 837, 1385, 1465, 1527, 1687
BseMI GCAATG 1 cut(s) 2071
BseMII CTCAG 7 cut(s) 192, 402, 935, 1133, 1980, 2343, 2406
BseNI ACTGG 7 cut(s) 61, 393, 460, 638, 1781, 1809, 2433
BseRI GAGGAG 2 cut(s) 1481, 1643
BseSI GKGCMC 2 cut(s) 276, 560
BseXI GCAGC 4 cut(s) 178, 1288, 1521, 2043
BsgI GTGCAG 1 cut(s) 383
Bsh1236I CGCG 1 cut(s) 1204
BshNI GGYRCC 1 cut(s) 1352
BsiHKAI GWGCWC 4 cut(s) 276, 720, 990, 1854
BsiSI CCGG 5 cut(s) 10, 1656, 1941, 2215, 2238
BslI CCNNNNNNNGG 7 cut(s) 88, 355, 837, 1385, 1465, 1527, 1687
BsmAI GTCTC 5 cut(s) 224, 591, 653, 1369, 2282
BsmBI CGTCTC 1 cut(s) 591
BsmI GAATGC 1 cut(s) 1754
Bso31I GGTCTC 2 cut(s) 224, 1369
Bsp1286I GDGCHC 6 cut(s) 276, 560, 720, 990, 1512, 1854
BspACI CCGC 8 cut(s) 295, 889, 1098, 1202, 1204, 1356, 1389, 1459
BspCNI CTCAG 7 cut(s) 193, 403, 936, 1134, 1981, 2344, 2407
BspFNI CGCG 1 cut(s) 1204
BspHI TCATGA 2 cut(s) 870, 958
BspLI GGNNCC 9 cut(s) 52, 458, 491, 1354, 1704, 2039, 2107, 2236, 2385
BspPI GGATC 6 cut(s) 451, 464, 527, 1126, 2356, 2456
BspQI GCTCTTC 1 cut(s) 725
BspT107I GGYRCC 1 cut(s) 1352
BspTNI GGTCTC 2 cut(s) 224, 1369
BsrDI GCAATG 1 cut(s) 2071
BsrI ACTGG 7 cut(s) 61, 393, 460, 638, 1781, 1809, 2433
BssECI CCNNGG 7 cut(s) 286, 798, 831, 1202, 1407, 1431, 1941
BssNI GRCGYC 1 cut(s) 975
BssSI CACGAG 1 cut(s) 1928
BssT1I CCWWGG 1 cut(s) 1407
Bst2BI CACGAG 1 cut(s) 1928
Bst2UI CCWGG 6 cut(s) 728, 799, 832, 1350, 1432, 1539
Bst6I CTCTTC 3 cut(s) 725, 1260, 2495
BstACI GRCGYC 1 cut(s) 975
BstAPI GCANNNNNTGC 1 cut(s) 1331
BstC8I GCNNGC 5 cut(s) 537, 1274, 1336, 1707, 2026
BstDSI CCRYGG 1 cut(s) 1202
BstFNI CGCG 1 cut(s) 1204
BstH2I RGCGCY 1 cut(s) 2477
BstHHI GCGC 3 cut(s) 914, 2061, 2476
BstMAI GTCTC 5 cut(s) 224, 591, 653, 1369, 2282
BstMWI GCNNNNNNNGC 5 cut(s) 59, 197, 271, 1061, 1331
BstNI CCWGG 6 cut(s) 728, 799, 832, 1350, 1432, 1539
BstNSI RCATGY 3 cut(s) 439, 1430, 1552
BstSFI CTRYAG 2 cut(s) 1563, 2145
BstSLI GKGCMC 2 cut(s) 276, 560
BstUI CGCG 1 cut(s) 1204
BstV1I GCAGC 4 cut(s) 178, 1288, 1521, 2043
BstV2I GAAGAC 1 cut(s) 438
BstX2I RGATCY 3 cut(s) 456, 1131, 2348
BstXI CCANNNNNNTGG 1 cut(s) 2394
BstYI RGATCY 3 cut(s) 456, 1131, 2348
Bsu36I CCTNAGG 2 cut(s) 2516, 2532
BtgI CCRYGG 1 cut(s) 1202
BtrI CACGTC 2 cut(s) 585, 1009
BtsIMutI CAGTG 4 cut(s) 54, 283, 2045, 2112
Cac8I GCNNGC 5 cut(s) 537, 1274, 1336, 1707, 2026
CaiI CAGNNNCTG 2 cut(s) 1190, 1331
CciI TCATGA 2 cut(s) 870, 958
CfoI GCGC 3 cut(s) 914, 2061, 2476
Cfr42I CCGCGG 1 cut(s) 1205
CseI GACGC 2 cut(s) 581, 624
Csp6I GTAC 4 cut(s) 463, 485, 1816, 2469
CspCI CAANNNNNGTGG 4 cut(s) 388, 423, 1053, 1088
CviQI GTAC 4 cut(s) 463, 485, 1816, 2469
EaeI YGGCCR 2 cut(s) 346, 1053
Eam1104I CTCTTC 3 cut(s) 725, 1260, 2495
EarI CTCTTC 3 cut(s) 725, 1260, 2495
Ecl136II GAGCTC 1 cut(s) 988
Eco130I CCWWGG 1 cut(s) 1407
Eco147I AGGCCT 1 cut(s) 1691
Eco24I GRGCYC 2 cut(s) 990, 1512
Eco31I GGTCTC 2 cut(s) 224, 1369
Eco47I GGWCC 7 cut(s) 251, 490, 1541, 2038, 2106, 2217, 2512
Eco53kI GAGCTC 1 cut(s) 988
Eco57I CTGAAG 1 cut(s) 494
Eco81I CCTNAGG 2 cut(s) 2516, 2532
EcoICRI GAGCTC 1 cut(s) 988
EcoO109I RGGNCCY 1 cut(s) 1541
EcoRII CCWGG 6 cut(s) 726, 797, 830, 1348, 1430, 1537
EcoT14I CCWWGG 1 cut(s) 1407
EcoT38I GRGCYC 2 cut(s) 990, 1512
ErhI CCWWGG 1 cut(s) 1407
Esp3I CGTCTC 1 cut(s) 591
FauI CCCGC 2 cut(s) 882, 1466
FauNDI CATATG 1 cut(s) 844
Fnu4HI GCNGC 6 cut(s) 192, 1205, 1277, 1356, 1535, 2057
FriOI GRGCYC 2 cut(s) 990, 1512
Fsp4HI GCNGC 6 cut(s) 192, 1205, 1277, 1356, 1535, 2057
FspBI CTAG 5 cut(s) 542, 662, 903, 1061, 1973
GlaI GCGC 3 cut(s) 913, 2060, 2475
GluI GCNGC 6 cut(s) 192, 1205, 1277, 1356, 1535, 2057
GsuI CTGGAG 4 cut(s) 78, 280, 710, 1280
HaeII RGCGCY 1 cut(s) 2477
HapII CCGG 5 cut(s) 10, 1656, 1941, 2215, 2238
HgaI GACGC 2 cut(s) 581, 624
HhaI GCGC 3 cut(s) 914, 2061, 2476
Hin1I GRCGYC 1 cut(s) 975
Hin6I GCGC 3 cut(s) 912, 2059, 2474
HinP1I GCGC 3 cut(s) 912, 2059, 2474
HincII GTYRAC 2 cut(s) 283, 580
HindII GTYRAC 2 cut(s) 283, 580
HpaII CCGG 5 cut(s) 10, 1656, 1941, 2215, 2238
Hpy166II GTNNAC 6 cut(s) 153, 274, 283, 580, 1180, 1443
Hpy8I GTNNAC 6 cut(s) 153, 274, 283, 580, 1180, 1443
Hpy99I CGWCG 2 cut(s) 509, 977
HpyCH4IV ACGT 5 cut(s) 405, 584, 975, 1008, 1176
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 197, 271, 1061, 1331
HpySE526I ACGT 5 cut(s) 405, 584, 975, 1008, 1176
Hsp92I GRCGYC 1 cut(s) 975
HspAI GCGC 3 cut(s) 912, 2059, 2474
KspI CCGCGG 1 cut(s) 1205
LguI GCTCTTC 1 cut(s) 725
LmnI GCTCC 6 cut(s) 50, 59, 985, 1502, 1849, 2383
Lsp1109I GCAGC 4 cut(s) 178, 1288, 1521, 2043
LweI GCATC 7 cut(s) 224, 1015, 1521, 1545, 1914, 2140, 2196
MaeI CTAG 5 cut(s) 542, 662, 903, 1061, 1973
MaeII ACGT 5 cut(s) 405, 584, 975, 1008, 1176
MaeIII GTNAC 9 cut(s) 127, 328, 440, 670, 1004, 1453, 1618, 2308, 2446
MfeI CAATTG 3 cut(s) 864, 2221, 2478
MflI RGATCY 3 cut(s) 456, 1131, 2348
MhlI GDGCHC 6 cut(s) 276, 560, 720, 990, 1512, 1854
MluCI AATT 6 cut(s) 133, 864, 1591, 1900, 2221, 2478
MlyI GAGTC 7 cut(s) 62, 165, 659, 868, 930, 2005, 2495
MmeI TCCRAC 4 cut(s) 229, 963, 1159, 1508
MroXI GAANNNNTTC 2 cut(s) 1833, 2526
MseI TTAA 2 cut(s) 855, 2199
MslI CAYNNNNRTG 2 cut(s) 1553, 1601
MspA1I CMGCKG 1 cut(s) 1204
MspI CCGG 5 cut(s) 10, 1656, 1941, 2215, 2238
MunI CAATTG 3 cut(s) 864, 2221, 2478
Mva1269I GAATGC 1 cut(s) 1754
MvaI CCWGG 6 cut(s) 728, 799, 832, 1350, 1432, 1539
MvnI CGCG 1 cut(s) 1204
MwoI GCNNNNNNNGC 5 cut(s) 59, 197, 271, 1061, 1331
NciI CCSGG 4 cut(s) 10, 1657, 1942, 2239
NdeI CATATG 1 cut(s) 844
NlaIV GGNNCC 9 cut(s) 52, 458, 491, 1354, 1704, 2039, 2107, 2236, 2385
NmeAIII GCCGAG 2 cut(s) 267, 734
NmuCI GTSAC 5 cut(s) 127, 440, 670, 1004, 1453
NspI RCATGY 3 cut(s) 439, 1430, 1552
PagI TCATGA 2 cut(s) 870, 958
PceI AGGCCT 1 cut(s) 1691
PciI ACATGT 1 cut(s) 435
PciSI GCTCTTC 1 cut(s) 725
PctI GAATGC 1 cut(s) 1754
PdmI GAANNNNTTC 2 cut(s) 1833, 2526
PfeI GAWTC 7 cut(s) 592, 1039, 1232, 1403, 1660, 1829, 1881
PflFI GACNNNGTC 1 cut(s) 2014
PflMI CCANNNNNTGG 1 cut(s) 1527
PkrI GCNGC 6 cut(s) 193, 1206, 1278, 1357, 1536, 2058
PleI GAGTC 7 cut(s) 62, 165, 659, 867, 930, 2005, 2494
PpsI GAGTC 7 cut(s) 62, 165, 659, 867, 930, 2005, 2494
PpuMI RGGWCCY 1 cut(s) 1541
PscI ACATGT 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 990
Psp1406I AACGTT 1 cut(s) 1176
Psp5II RGGWCCY 1 cut(s) 1541
Psp6I CCWGG 6 cut(s) 726, 797, 830, 1348, 1430, 1537
PspGI CCWGG 6 cut(s) 726, 797, 830, 1348, 1430, 1537
PspN4I GGNNCC 9 cut(s) 52, 458, 491, 1354, 1704, 2039, 2107, 2236, 2385
PspPPI RGGWCCY 1 cut(s) 1541
PstNI CAGNNNCTG 2 cut(s) 1190, 1331
PsuI RGATCY 3 cut(s) 456, 1131, 2348
PsyI GACNNNGTC 1 cut(s) 2014
RsaI GTAC 4 cut(s) 464, 486, 1817, 2470
RsaNI GTAC 4 cut(s) 463, 485, 1816, 2469
RseI CAYNNNNRTG 2 cut(s) 1553, 1601
SacI GAGCTC 1 cut(s) 990
SacII CCGCGG 1 cut(s) 1205
SapI GCTCTTC 1 cut(s) 725
SaqAI TTAA 2 cut(s) 855, 2199
SatI GCNGC 6 cut(s) 192, 1205, 1277, 1356, 1535, 2057
ScaI AGTACT 1 cut(s) 464
SchI GAGTC 7 cut(s) 62, 165, 659, 868, 930, 2005, 2495
SduI GDGCHC 6 cut(s) 276, 560, 720, 990, 1512, 1854
SfaNI GCATC 7 cut(s) 224, 1015, 1521, 1545, 1914, 2140, 2196
SfcI CTRYAG 2 cut(s) 1563, 2145
Sfr303I CCGCGG 1 cut(s) 1205
SgrBI CCGCGG 1 cut(s) 1205
SinI GGWCC 7 cut(s) 251, 490, 1541, 2038, 2106, 2217, 2512
SmiMI CAYNNNNRTG 2 cut(s) 1553, 1601
SmlI CTYRAG 2 cut(s) 1339, 2451
SmoI CTYRAG 2 cut(s) 1339, 2451
Sse9I AATT 6 cut(s) 133, 864, 1591, 1900, 2221, 2478
SseBI AGGCCT 1 cut(s) 1691
SsiI CCGC 8 cut(s) 295, 889, 1098, 1202, 1204, 1356, 1389, 1459
SspMI CTAG 5 cut(s) 542, 662, 903, 1061, 1973
SstI GAGCTC 1 cut(s) 990
StuI AGGCCT 1 cut(s) 1691
StyI CCWWGG 1 cut(s) 1407
TaiI ACGT 5 cut(s) 408, 587, 978, 1011, 1179
TaqI TCGA 4 cut(s) 305, 741, 972, 2175
TasI AATT 6 cut(s) 133, 864, 1591, 1900, 2221, 2478
TatI WGTACW 2 cut(s) 462, 2468
TauI GCSGC 2 cut(s) 1207, 1358
TfiI GAWTC 7 cut(s) 592, 1039, 1232, 1403, 1660, 1829, 1881
Tru1I TTAA 2 cut(s) 855, 2199
Tru9I TTAA 2 cut(s) 855, 2199
TscAI CASTG 4 cut(s) 61, 283, 2052, 2119
TseFI GTSAC 5 cut(s) 127, 440, 670, 1004, 1453
TseI GCWGC 4 cut(s) 191, 1276, 1534, 2056
Tsp45I GTSAC 5 cut(s) 127, 440, 670, 1004, 1453
TspDTI ATGAA 8 cut(s) 17, 755, 947, 1298, 1591, 1910, 2378, 2439
TspGWI ACGGA 2 cut(s) 166, 2534
TspRI CASTG 4 cut(s) 61, 283, 2052, 2119
Tth111I GACNNNGTC 1 cut(s) 2014
Van91I CCANNNNNTGG 1 cut(s) 1527
VneI GTGCAC 1 cut(s) 272
VpaK11BI GGWCC 7 cut(s) 251, 490, 1541, 2038, 2106, 2217, 2512
XapI RAATTY 1 cut(s) 133
XbaI TCTAGA 1 cut(s) 1972
XceI RCATGY 3 cut(s) 439, 1430, 1552
XcmI CCANNNNNNNNNTGG 3 cut(s) 202, 686, 795
XmnI GAANNNNTTC 2 cut(s) 1833, 2526
XspI CTAG 5 cut(s) 542, 662, 903, 1061, 1973
ZraI GACGTC 1 cut(s) 976
ZrmI AGTACT 1 cut(s) 464
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.