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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 64, 1343 |
| AatII | GACGTC | 2 cut(s) | 389, 1235 |
| Acc36I | ACCTGC | 1 cut(s) | 952 |
| Acc65I | GGTACC | 1 cut(s) | 338 |
| AccB1I | GGYRCC | 3 cut(s) | 338, 855, 891 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 826 |
| AccI | GTMKAC | 3 cut(s) | 535, 1554, 1680 |
| AccII | CGCG | 3 cut(s) | 8, 10, 570 |
| AciI | CCGC | 8 cut(s) | 10, 135, 528, 840, 1147, 1172, 1226, 1739 |
| AclWI | GGATC | 2 cut(s) | 25, 1716 |
| AcoI | YGGCCR | 2 cut(s) | 132, 915 |
| AcuI | CTGAAG | 2 cut(s) | 905, 981 |
| AcvI | CACGTG | 1 cut(s) | 229 |
| AcyI | GRCGYC | 2 cut(s) | 386, 1232 |
| AfaI | GTAC | 3 cut(s) | 340, 372, 1482 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 360, 618, 826, 1089, 1272, 1375, 1865, 1906 |
| AflIII | ACRYGT | 2 cut(s) | 228, 1872 |
| AgeI | ACCGGT | 1 cut(s) | 852 |
| AgsI | TTSAA | 5 cut(s) | 235, 671, 1294, 1608, 1880 |
| AhlI | ACTAGT | 1 cut(s) | 1616 |
| AjnI | CCWGG | 3 cut(s) | 1321, 1832, 1864 |
| AluBI | AGCT | 5 cut(s) | 271, 510, 667, 1005, 1508 |
| AluI | AGCT | 5 cut(s) | 271, 510, 667, 1005, 1508 |
| Alw21I | GWGCWC | 2 cut(s) | 255, 273 |
| Alw26I | GTCTC | 6 cut(s) | 199, 299, 422, 1223, 1463, 1704 |
| AlwI | GGATC | 2 cut(s) | 25, 1716 |
| Ama87I | CYCGRG | 1 cut(s) | 425 |
| AoxI | GGCC | 7 cut(s) | 132, 279, 433, 816, 915, 1365, 1422 |
| ApeKI | GCWGC | 4 cut(s) | 992, 1358, 1768, 1771 |
| AsiGI | ACCGGT | 1 cut(s) | 852 |
| Asp718I | GGTACC | 1 cut(s) | 338 |
| AspLEI | GCGC | 7 cut(s) | 8, 10, 497, 570, 1022, 1321, 1660 |
| AspS9I | GGNCC | 5 cut(s) | 194, 279, 817, 1169, 1366 |
| AsuC2I | CCSGG | 1 cut(s) | 1017 |
| AsuHPI | GGTGA | 1 cut(s) | 1777 |
| AvaI | CYCGRG | 1 cut(s) | 425 |
| AvaII | GGWCC | 2 cut(s) | 194, 1169 |
| BaeGI | GKGCMC | 2 cut(s) | 636, 1536 |
| BanI | GGYRCC | 3 cut(s) | 338, 855, 891 |
| BanII | GRGCYC | 1 cut(s) | 273 |
| BbrPI | CACGTG | 1 cut(s) | 229 |
| BbsI | GAAGAC | 2 cut(s) | 801, 1040 |
| Bbv12I | GWGCWC | 2 cut(s) | 255, 273 |
| BbvCI | CCTCAGC | 1 cut(s) | 1245 |
| BbvI | GCAGC | 4 cut(s) | 979, 1370, 1755, 1758 |
| BccI | CCATC | 5 cut(s) | 802, 1092, 1174, 1494, 1927 |
| BceAI | ACGGC | 1 cut(s) | 473 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1753, 1787 |
| BciT130I | CCWGG | 3 cut(s) | 1323, 1834, 1866 |
| BclI | TGATCA | 1 cut(s) | 213 |
| BcnI | CCSGG | 1 cut(s) | 1017 |
| BcoDI | GTCTC | 6 cut(s) | 199, 299, 422, 1223, 1463, 1704 |
| BcuI | ACTAGT | 1 cut(s) | 1616 |
| BfaI | CTAG | 3 cut(s) | 554, 987, 1617 |
| BfmI | CTRYAG | 1 cut(s) | 105 |
| BfoI | RGCGCY | 1 cut(s) | 498 |
| BfuAI | ACCTGC | 1 cut(s) | 952 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 923 |
| BglII | AGATCT | 1 cut(s) | 82 |
| BisI | GCNGC | 7 cut(s) | 11, 135, 841, 993, 1359, 1769, 1772 |
| BlsI | GCNGC | 7 cut(s) | 12, 136, 842, 994, 1360, 1770, 1773 |
| Bme1390I | CCNGG | 4 cut(s) | 1017, 1323, 1834, 1866 |
| Bme18I | GGWCC | 2 cut(s) | 194, 1169 |
| BmeT110I | CYCGRG | 1 cut(s) | 425 |
| BmgT120I | GGNCC | 5 cut(s) | 194, 279, 817, 1169, 1366 |
| BmiI | GGNNCC | 6 cut(s) | 280, 340, 610, 851, 857, 893 |
| BmrFI | CCNGG | 4 cut(s) | 1017, 1323, 1834, 1866 |
| BmrI | ACTGGG | 3 cut(s) | 463, 1281, 1508 |
| BmsI | GCATC | 5 cut(s) | 467, 475, 940, 1809, 1856 |
| BmuI | ACTGGG | 3 cut(s) | 463, 1281, 1508 |
| BpiI | GAAGAC | 2 cut(s) | 801, 1040 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 38 |
| BpmI | CTGGAG | 2 cut(s) | 991, 1509 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1245 |
| BpuEI | CTTGAG | 4 cut(s) | 1049, 1447, 1760, 1844 |
| BpuMI | CCSGG | 1 cut(s) | 1017 |
| Bsa29I | ATCGAT | 1 cut(s) | 1350 |
| BsaAI | YACGTR | 1 cut(s) | 229 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1918 |
| BsaHI | GRCGYC | 2 cut(s) | 386, 1232 |
| BsaI | GGTCTC | 1 cut(s) | 1463 |
| BsaJI | CCNNGG | 5 cut(s) | 1221, 1322, 1374, 1833, 1864 |
| BsaWI | WCCGGW | 1 cut(s) | 852 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 266, 296 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 360, 618, 826, 1089, 1272, 1375, 1865, 1906 |
| Bse118I | RCCGGY | 2 cut(s) | 110, 852 |
| Bse1I | ACTGG | 9 cut(s) | 458, 741, 1066, 1276, 1368, 1492, 1514, 1541, 1733 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1918 |
| BseBI | CCWGG | 3 cut(s) | 1323, 1834, 1866 |
| BseCI | ATCGAT | 1 cut(s) | 1350 |
| BseDI | CCNNGG | 5 cut(s) | 1221, 1322, 1374, 1833, 1864 |
| BseGI | GGATG | 4 cut(s) | 411, 490, 955, 1505 |
| BseJI | GATNNNNATC | 1 cut(s) | 1918 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 360, 618, 826, 1089, 1272, 1375, 1865, 1906 |
| BseMII | CTCAG | 4 cut(s) | 1230, 1236, 1407, 1635 |
| BseNI | ACTGG | 9 cut(s) | 458, 741, 1066, 1276, 1368, 1492, 1514, 1541, 1733 |
| BsePI | GCGCGC | 1 cut(s) | 6 |
| BseRI | GAGGAG | 5 cut(s) | 39, 155, 583, 1481, 1825 |
| BseSI | GKGCMC | 2 cut(s) | 636, 1536 |
| BseXI | GCAGC | 4 cut(s) | 979, 1370, 1755, 1758 |
| BsgI | GTGCAG | 1 cut(s) | 893 |
| Bsh1236I | CGCG | 3 cut(s) | 8, 10, 570 |
| BshFI | GGCC | 7 cut(s) | 134, 281, 435, 818, 917, 1367, 1424 |
| BshNI | GGYRCC | 3 cut(s) | 338, 855, 891 |
| BshTI | ACCGGT | 1 cut(s) | 852 |
| BshVI | ATCGAT | 1 cut(s) | 1350 |
| BsiHKAI | GWGCWC | 2 cut(s) | 255, 273 |
| BsiHKCI | CYCGRG | 1 cut(s) | 425 |
| BsiSI | CCGG | 3 cut(s) | 111, 853, 1016 |
| BslFI | GGGAC | 6 cut(s) | 170, 235, 323, 391, 1146, 1598 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 360, 618, 826, 1089, 1272, 1375, 1865, 1906 |
| BsmAI | GTCTC | 6 cut(s) | 199, 299, 422, 1223, 1463, 1704 |
| BsmBI | CGTCTC | 2 cut(s) | 299, 1223 |
| BsmFI | GGGAC | 6 cut(s) | 170, 235, 323, 391, 1146, 1598 |
| BsnI | GGCC | 7 cut(s) | 134, 281, 435, 818, 917, 1367, 1424 |
| Bso31I | GGTCTC | 1 cut(s) | 1463 |
| BsoBI | CYCGRG | 1 cut(s) | 425 |
| Bsp1286I | GDGCHC | 4 cut(s) | 255, 273, 636, 1536 |
| Bsp143I | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| Bsp19I | CCATGG | 1 cut(s) | 1221 |
| BspACI | CCGC | 8 cut(s) | 10, 135, 528, 840, 1147, 1172, 1226, 1739 |
| BspANI | GGCC | 7 cut(s) | 134, 281, 435, 818, 917, 1367, 1424 |
| BspCNI | CTCAG | 4 cut(s) | 1231, 1237, 1408, 1636 |
| BspDI | ATCGAT | 1 cut(s) | 1350 |
| BspFNI | CGCG | 3 cut(s) | 8, 10, 570 |
| BspHI | TCATGA | 1 cut(s) | 1894 |
| BspLI | GGNNCC | 6 cut(s) | 280, 340, 610, 851, 857, 893 |
| BspMI | ACCTGC | 1 cut(s) | 952 |
| BspPI | GGATC | 2 cut(s) | 25, 1716 |
| BspQI | GCTCTTC | 1 cut(s) | 747 |
| BspT107I | GGYRCC | 3 cut(s) | 338, 855, 891 |
| BspTNI | GGTCTC | 1 cut(s) | 1463 |
| BsrFI | RCCGGY | 2 cut(s) | 110, 852 |
| BsrI | ACTGG | 9 cut(s) | 458, 741, 1066, 1276, 1368, 1492, 1514, 1541, 1733 |
| BssAI | RCCGGY | 2 cut(s) | 110, 852 |
| BssECI | CCNNGG | 5 cut(s) | 1221, 1322, 1374, 1833, 1864 |
| BssHII | GCGCGC | 1 cut(s) | 6 |
| BssMI | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| BssNI | GRCGYC | 2 cut(s) | 386, 1232 |
| BssT1I | CCWWGG | 2 cut(s) | 1221, 1374 |
| Bst2UI | CCWGG | 3 cut(s) | 1323, 1834, 1866 |
| Bst4CI | ACNGT | 6 cut(s) | 106, 800, 871, 946, 1136, 1573 |
| Bst6I | CTCTTC | 4 cut(s) | 29, 426, 747, 881 |
| BstACI | GRCGYC | 2 cut(s) | 386, 1232 |
| BstBAI | YACGTR | 1 cut(s) | 229 |
| BstC8I | GCNNGC | 4 cut(s) | 8, 928, 1003, 1263 |
| BstDEI | CTNAG | 6 cut(s) | 704, 1239, 1245, 1416, 1433, 1644 |
| BstDSI | CCRYGG | 1 cut(s) | 1221 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| BstF5I | GGATG | 4 cut(s) | 411, 490, 955, 1505 |
| BstFNI | CGCG | 3 cut(s) | 8, 10, 570 |
| BstH2I | RGCGCY | 1 cut(s) | 498 |
| BstHHI | GCGC | 7 cut(s) | 8, 10, 497, 570, 1022, 1321, 1660 |
| BstKTI | GATC | 9 cut(s) | 20, 80, 85, 216, 331, 780, 1046, 1713, 1724 |
| BstMAI | GTCTC | 6 cut(s) | 199, 299, 422, 1223, 1463, 1704 |
| BstMBI | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 107, 131, 494, 923, 998, 1017, 1079, 1364, 1421 |
| BstNI | CCWGG | 3 cut(s) | 1323, 1834, 1866 |
| BstNSI | RCATGY | 1 cut(s) | 1876 |
| BstSCI | CCNGG | 4 cut(s) | 1015, 1321, 1832, 1864 |
| BstSFI | CTRYAG | 1 cut(s) | 105 |
| BstSLI | GKGCMC | 2 cut(s) | 636, 1536 |
| BstUI | CGCG | 3 cut(s) | 8, 10, 570 |
| BstV1I | GCAGC | 4 cut(s) | 979, 1370, 1755, 1758 |
| BstV2I | GAAGAC | 2 cut(s) | 801, 1040 |
| BstX2I | RGATCY | 3 cut(s) | 17, 82, 1721 |
| BstYI | RGATCY | 3 cut(s) | 17, 82, 1721 |
| Bsu15I | ATCGAT | 1 cut(s) | 1350 |
| BsuRI | GGCC | 7 cut(s) | 134, 281, 435, 818, 917, 1367, 1424 |
| BsuTUI | ATCGAT | 1 cut(s) | 1350 |
| BtgI | CCRYGG | 1 cut(s) | 1221 |
| BtsCI | GGATG | 4 cut(s) | 411, 490, 955, 1505 |
| BtsIMutI | CAGTG | 8 cut(s) | 102, 876, 881, 1236, 1361, 1449, 1521, 1534 |
| BveI | ACCTGC | 1 cut(s) | 952 |
| Cac8I | GCNNGC | 4 cut(s) | 8, 928, 1003, 1263 |
| CciI | TCATGA | 1 cut(s) | 1894 |
| CfoI | GCGC | 7 cut(s) | 8, 10, 497, 570, 1022, 1321, 1660 |
| Cfr10I | RCCGGY | 2 cut(s) | 110, 852 |
| Cfr13I | GGNCC | 5 cut(s) | 194, 279, 817, 1169, 1366 |
| ClaI | ATCGAT | 1 cut(s) | 1350 |
| CpoI | CGGWCCG | 1 cut(s) | 1169 |
| CseI | GACGC | 2 cut(s) | 281, 559 |
| Csp6I | GTAC | 3 cut(s) | 339, 371, 1481 |
| CspAI | ACCGGT | 1 cut(s) | 852 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 17, 52, 1891, 1926 |
| CspI | CGGWCCG | 1 cut(s) | 1169 |
| CviQI | GTAC | 3 cut(s) | 339, 371, 1481 |
| DdeI | CTNAG | 6 cut(s) | 704, 1239, 1245, 1416, 1433, 1644 |
| DpnI | GATC | 9 cut(s) | 19, 79, 84, 215, 330, 779, 1045, 1712, 1723 |
| DpnII | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 64, 1343 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 64, 1343 |
| EaeI | YGGCCR | 2 cut(s) | 132, 915 |
| Eam1104I | CTCTTC | 4 cut(s) | 29, 426, 747, 881 |
| EarI | CTCTTC | 4 cut(s) | 29, 426, 747, 881 |
| EciI | GGCGGA | 2 cut(s) | 1241, 1754 |
| Ecl136II | GAGCTC | 1 cut(s) | 271 |
| Eco130I | CCWWGG | 2 cut(s) | 1221, 1374 |
| Eco147I | AGGCCT | 1 cut(s) | 1424 |
| Eco24I | GRGCYC | 1 cut(s) | 273 |
| Eco31I | GGTCTC | 1 cut(s) | 1463 |
| Eco47I | GGWCC | 2 cut(s) | 194, 1169 |
| Eco53kI | GAGCTC | 1 cut(s) | 271 |
| Eco57I | CTGAAG | 2 cut(s) | 905, 981 |
| Eco72I | CACGTG | 1 cut(s) | 229 |
| Eco88I | CYCGRG | 1 cut(s) | 425 |
| EcoICRI | GAGCTC | 1 cut(s) | 271 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| EcoO109I | RGGNCCY | 1 cut(s) | 279 |
| EcoRII | CCWGG | 3 cut(s) | 1321, 1832, 1864 |
| EcoT14I | CCWWGG | 2 cut(s) | 1221, 1374 |
| EcoT38I | GRGCYC | 1 cut(s) | 273 |
| ErhI | CCWWGG | 2 cut(s) | 1221, 1374 |
| Esp3I | CGTCTC | 2 cut(s) | 299, 1223 |
| FaqI | GGGAC | 6 cut(s) | 170, 235, 323, 391, 1146, 1598 |
| FauI | CCCGC | 1 cut(s) | 521 |
| FauNDI | CATATG | 1 cut(s) | 1105 |
| FbaI | TGATCA | 1 cut(s) | 213 |
| FblI | GTMKAC | 3 cut(s) | 535, 1554, 1680 |
| Fnu4HI | GCNGC | 7 cut(s) | 11, 135, 841, 993, 1359, 1769, 1772 |
| FokI | GGATG | 4 cut(s) | 398, 497, 962, 1512 |
| FriOI | GRGCYC | 1 cut(s) | 273 |
| Fsp4HI | GCNGC | 7 cut(s) | 11, 135, 841, 993, 1359, 1769, 1772 |
| FspBI | CTAG | 3 cut(s) | 554, 987, 1617 |
| GlaI | GCGC | 7 cut(s) | 7, 9, 496, 569, 1021, 1320, 1659 |
| GluI | GCNGC | 7 cut(s) | 11, 135, 841, 993, 1359, 1769, 1772 |
| GsuI | CTGGAG | 2 cut(s) | 991, 1509 |
| HaeII | RGCGCY | 1 cut(s) | 498 |
| HaeIII | GGCC | 7 cut(s) | 134, 281, 435, 818, 917, 1367, 1424 |
| HapII | CCGG | 3 cut(s) | 111, 853, 1016 |
| HgaI | GACGC | 2 cut(s) | 281, 559 |
| HhaI | GCGC | 7 cut(s) | 8, 10, 497, 570, 1022, 1321, 1660 |
| Hin1I | GRCGYC | 2 cut(s) | 386, 1232 |
| Hin6I | GCGC | 7 cut(s) | 6, 8, 495, 568, 1020, 1319, 1658 |
| HinP1I | GCGC | 7 cut(s) | 6, 8, 495, 568, 1020, 1319, 1658 |
| HincII | GTYRAC | 2 cut(s) | 400, 643 |
| HindII | GTYRAC | 2 cut(s) | 400, 643 |
| HindIII | AAGCTT | 1 cut(s) | 665 |
| HinfI | GANTC | 8 cut(s) | 65, 158, 223, 700, 724, 877, 1540, 1891 |
| HpaII | CCGG | 3 cut(s) | 111, 853, 1016 |
| HphI | GGTGA | 1 cut(s) | 1777 |
| Hpy166II | GTNNAC | 8 cut(s) | 400, 536, 643, 1101, 1236, 1336, 1555, 1681 |
| Hpy188I | TCNGA | 6 cut(s) | 82, 257, 443, 598, 1140, 1645 |
| Hpy188III | TCNNGA | 6 cut(s) | 425, 427, 574, 1028, 1047, 1895 |
| Hpy8I | GTNNAC | 8 cut(s) | 400, 536, 643, 1101, 1236, 1336, 1555, 1681 |
| Hpy99I | CGWCG | 5 cut(s) | 101, 194, 385, 388, 1199 |
| HpyAV | CCTTC | 8 cut(s) | 121, 468, 1049, 1245, 1271, 1294, 1414, 1736 |
| HpyCH4III | ACNGT | 6 cut(s) | 106, 800, 871, 946, 1136, 1573 |
| HpyCH4IV | ACGT | 7 cut(s) | 228, 386, 910, 1232, 1344, 1640, 1677 |
| HpyCH4V | TGCA | 6 cut(s) | 874, 953, 1109, 1519, 1847, 1935 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 107, 131, 494, 923, 998, 1017, 1079, 1364, 1421 |
| HpyF3I | CTNAG | 6 cut(s) | 704, 1239, 1245, 1416, 1433, 1644 |
| HpySE526I | ACGT | 7 cut(s) | 228, 386, 910, 1232, 1344, 1640, 1677 |
| Hsp92I | GRCGYC | 2 cut(s) | 386, 1232 |
| HspAI | GCGC | 7 cut(s) | 6, 8, 495, 568, 1020, 1319, 1658 |
| KpnI | GGTACC | 1 cut(s) | 342 |
| Ksp22I | TGATCA | 1 cut(s) | 213 |
| Kzo9I | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| LguI | GCTCTTC | 1 cut(s) | 747 |
| LmnI | GCTCC | 3 cut(s) | 608, 958, 1010 |
| Lsp1109I | GCAGC | 4 cut(s) | 979, 1370, 1755, 1758 |
| LweI | GCATC | 5 cut(s) | 467, 475, 940, 1809, 1856 |
| MaeI | CTAG | 3 cut(s) | 554, 987, 1617 |
| MaeII | ACGT | 7 cut(s) | 228, 386, 910, 1232, 1344, 1640, 1677 |
| MaeIII | GTNAC | 4 cut(s) | 139, 549, 982, 1868 |
| MalI | GATC | 9 cut(s) | 19, 79, 84, 215, 330, 779, 1045, 1712, 1723 |
| MboI | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| MboII | GAAGA | 9 cut(s) | 16, 413, 734, 806, 817, 898, 1045, 1053, 1892 |
| MfeI | CAATTG | 2 cut(s) | 175, 821 |
| MflI | RGATCY | 3 cut(s) | 17, 82, 1721 |
| MhlI | GDGCHC | 4 cut(s) | 255, 273, 636, 1536 |
| MluCI | AATT | 5 cut(s) | 175, 586, 821, 971, 1213 |
| MlyI | GAGTC | 4 cut(s) | 74, 152, 217, 871 |
| MmeI | TCCRAC | 5 cut(s) | 462, 621, 1427, 1601, 1749 |
| MseI | TTAA | 3 cut(s) | 662, 785, 966 |
| MslI | CAYNNNNRTG | 1 cut(s) | 785 |
| MspA1I | CMGCKG | 2 cut(s) | 137, 1174 |
| MspI | CCGG | 3 cut(s) | 111, 853, 1016 |
| MspR9I | CCNGG | 4 cut(s) | 1017, 1323, 1834, 1866 |
| MunI | CAATTG | 2 cut(s) | 175, 821 |
| MvaI | CCWGG | 3 cut(s) | 1323, 1834, 1866 |
| MvnI | CGCG | 3 cut(s) | 8, 10, 570 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 107, 131, 494, 923, 998, 1017, 1079, 1364, 1421 |
| NciI | CCSGG | 1 cut(s) | 1017 |
| NcoI | CCATGG | 1 cut(s) | 1221 |
| NdeI | CATATG | 1 cut(s) | 1105 |
| NdeII | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| NlaIV | GGNNCC | 6 cut(s) | 280, 340, 610, 851, 857, 893 |
| NspI | RCATGY | 1 cut(s) | 1876 |
| PaeR7I | CTCGAG | 1 cut(s) | 425 |
| PagI | TCATGA | 1 cut(s) | 1894 |
| PauI | GCGCGC | 1 cut(s) | 6 |
| PceI | AGGCCT | 1 cut(s) | 1424 |
| PciI | ACATGT | 1 cut(s) | 1872 |
| PciSI | GCTCTTC | 1 cut(s) | 747 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 318, 1683 |
| PfeI | GAWTC | 4 cut(s) | 700, 724, 1540, 1891 |
| PflFI | GACNNNGTC | 2 cut(s) | 95, 385 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 826 |
| PinAI | ACCGGT | 1 cut(s) | 852 |
| PkrI | GCNGC | 7 cut(s) | 12, 136, 842, 994, 1360, 1770, 1773 |
| PleI | GAGTC | 4 cut(s) | 73, 152, 217, 871 |
| PmaCI | CACGTG | 1 cut(s) | 229 |
| PmlI | CACGTG | 1 cut(s) | 229 |
| PpsI | GAGTC | 4 cut(s) | 73, 152, 217, 871 |
| Ppu21I | YACGTR | 1 cut(s) | 229 |
| PscI | ACATGT | 1 cut(s) | 1872 |
| Psp124BI | GAGCTC | 1 cut(s) | 273 |
| Psp6I | CCWGG | 3 cut(s) | 1321, 1832, 1864 |
| PspCI | CACGTG | 1 cut(s) | 229 |
| PspGI | CCWGG | 3 cut(s) | 1321, 1832, 1864 |
| PspN4I | GGNNCC | 6 cut(s) | 280, 340, 610, 851, 857, 893 |
| PspPI | GGNCC | 5 cut(s) | 194, 279, 817, 1169, 1366 |
| PsuI | RGATCY | 3 cut(s) | 17, 82, 1721 |
| PsyI | GACNNNGTC | 2 cut(s) | 95, 385 |
| PteI | GCGCGC | 1 cut(s) | 6 |
| RsaI | GTAC | 3 cut(s) | 340, 372, 1482 |
| RsaNI | GTAC | 3 cut(s) | 339, 371, 1481 |
| RseI | CAYNNNNRTG | 1 cut(s) | 785 |
| Rsr2I | CGGWCCG | 1 cut(s) | 1169 |
| RsrII | CGGWCCG | 1 cut(s) | 1169 |
| SacI | GAGCTC | 1 cut(s) | 273 |
| SapI | GCTCTTC | 1 cut(s) | 747 |
| SaqAI | TTAA | 3 cut(s) | 662, 785, 966 |
| SatI | GCNGC | 7 cut(s) | 11, 135, 841, 993, 1359, 1769, 1772 |
| Sau3AI | GATC | 9 cut(s) | 17, 77, 82, 213, 328, 777, 1043, 1710, 1721 |
| Sau96I | GGNCC | 5 cut(s) | 194, 279, 817, 1169, 1366 |
| SchI | GAGTC | 4 cut(s) | 74, 152, 217, 871 |
| ScrFI | CCNGG | 4 cut(s) | 1017, 1323, 1834, 1866 |
| SduI | GDGCHC | 4 cut(s) | 255, 273, 636, 1536 |
| SfaNI | GCATC | 5 cut(s) | 467, 475, 940, 1809, 1856 |
| SfcI | CTRYAG | 1 cut(s) | 105 |
| Sfr274I | CTCGAG | 1 cut(s) | 425 |
| SinI | GGWCC | 2 cut(s) | 194, 1169 |
| SlaI | CTCGAG | 1 cut(s) | 425 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 785 |
| SmlI | CTYRAG | 5 cut(s) | 425, 1028, 1426, 1775, 1823 |
| SmoI | CTYRAG | 5 cut(s) | 425, 1028, 1426, 1775, 1823 |
| SpeI | ACTAGT | 1 cut(s) | 1616 |
| Sse9I | AATT | 5 cut(s) | 175, 586, 821, 971, 1213 |
| SseBI | AGGCCT | 1 cut(s) | 1424 |
| SsiI | CCGC | 8 cut(s) | 10, 135, 528, 840, 1147, 1172, 1226, 1739 |
| SspI | AATATT | 2 cut(s) | 1290, 1627 |
| SspMI | CTAG | 3 cut(s) | 554, 987, 1617 |
| SstI | GAGCTC | 1 cut(s) | 273 |
| StuI | AGGCCT | 1 cut(s) | 1424 |
| StyD4I | CCNGG | 4 cut(s) | 1015, 1321, 1832, 1864 |
| StyI | CCWWGG | 2 cut(s) | 1221, 1374 |
| TaaI | ACNGT | 6 cut(s) | 106, 800, 871, 946, 1136, 1573 |
| TaiI | ACGT | 7 cut(s) | 231, 389, 913, 1235, 1347, 1643, 1680 |
| TaqI | TCGA | 5 cut(s) | 52, 327, 426, 657, 1350 |
| TaqII | GACCGA | 1 cut(s) | 182 |
| TasI | AATT | 5 cut(s) | 175, 586, 821, 971, 1213 |
| TauI | GCSGC | 3 cut(s) | 13, 137, 843 |
| TfiI | GAWTC | 4 cut(s) | 700, 724, 1540, 1891 |
| Tru1I | TTAA | 3 cut(s) | 662, 785, 966 |
| Tru9I | TTAA | 3 cut(s) | 662, 785, 966 |
| TscAI | CASTG | 8 cut(s) | 109, 876, 888, 1243, 1368, 1456, 1521, 1541 |
| TseI | GCWGC | 4 cut(s) | 992, 1358, 1768, 1771 |
| TspDTI | ATGAA | 7 cut(s) | 629, 1157, 1299, 1401, 1767, 1811, 1883 |
| TspGWI | ACGGA | 3 cut(s) | 621, 1182, 1498 |
| TspRI | CASTG | 8 cut(s) | 109, 876, 888, 1243, 1368, 1456, 1521, 1541 |
| Tth111I | GACNNNGTC | 2 cut(s) | 95, 385 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 826 |
| VpaK11BI | GGWCC | 2 cut(s) | 194, 1169 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1373 |
| XceI | RCATGY | 1 cut(s) | 1876 |
| XhoI | CTCGAG | 1 cut(s) | 425 |
| XmiI | GTMKAC | 3 cut(s) | 535, 1554, 1680 |
| XspI | CTAG | 3 cut(s) | 554, 987, 1617 |
| ZraI | GACGTC | 2 cut(s) | 387, 1233 |