Prupe.3G135000_v2.0.a1

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0000194
Physical Location & Seq
Forward (+)
14208845 .. 14212755
3911 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G135000.1

Sequence Viewer

Length: 2811 bp
ATGCGCAATTTTAGGAATTTTGTGGCCAACTGCGGGTTGCTCGATTTAGGGTTTGTTGGTTACCCTTTTACTTGGAGCAATAGGCGAGAGGGAGAGCGTATACAAGAACGCCTTGACCGCGCTTTAGCATCATCCTCCTGGGTGCATCGCTTTGGGGAGGCCAAGGTTTCACACCACTTGGTATCAGGCTCTGATCATGCTGTGATCCTCTTGGAAGTGGAGCCAGTCCAGGTGACACACCGAGGAAGATTTATTTATGATCACAGGTGGGGTAAGGAAGTGGGTTGCAGAACAGCTGTACAGGATAGTTGGAGGTGGAAATGTAGGGGCTCTGCGTGTTATCGACTGGTGGAAAAATTAAAGGCAGCGCGGGGGAATATGCGGCAATGGAGGAGAGGTGCCAAAACAAACACGCAATGTAGAATTCGGGAGATTAATAAGGAACTCCAAATAAGTTATCAAGCCCCGGGTTTTGATCGGTCTAAGGTTTGTGCGCTAGAACGAGATCTGAAAGTGGGTGATAAGAATACGAAGTTTTTTCATGCCAAAACTGTGACGCGGCGGCGACGAAATAAAATTAGAGGCTTGGAGAATGAATTGGGGGAATGGCATGATGATGAGAAGGGGATACAAGACATCGCAGTTAAGTATTTTGACCAGCTCTTCACATCTGCAGCTTCTCTCCAGTTTGAGGAGATTATTAACTGTGTGGATCATCGCGTATCGGAACAGCACAATTTTGAACTCACCCGAGAAGTTACAGCGACTGAAATTAAAGAGGCTATATTTCAAATCCCTCCAACCAGGCCAGATGGCCTCACTGGGGAGGATGTGGTAGCTGCAGTTTTGGCATTCTTCCGAACAGGGTATATTTTGAAGAAAATTAATCATACCCATGTTGTGTTAATCCCAAAGGTGGCGAGTCCTACTCGAATGACACAACTACGACCAATCTCGTTATGTAATGTCATATATAAAGTGATATCGAAGGTCTTAACGAACCGCCTGAAACGGGTGCTTCCCCACTTTATTAGCCCGAATCAGACGGCATTTGTCTCCGGTCAGCAAATAACAGACAATGTCTTGATTGTCCATAAAGTACTCCATTCGTTAAAGACAAAAACACGTGGGTTGGAGTCTGATGTCGTGGTTAAGTTGGATATGGCGAAAGCTTACGATCGTGTGGAATGGCCCTTTCTTCTGGCCATGATGCGAAAGTTGGGTATTATGGTGAATGGTACGCCGACGGGTTTCATCCAGCCCCAACGAGGCTTGCGGCAAGGGGACCCATTGTCTCCATATTTGTTCTTGATTTGCACGGAGGGAATGACAGCGCTGATACAACACTTTGCGGGGAGGGAGCTGTTGCATGGTATTAAGGTGTCCAGCAGCAGCCCACCTCTCTCCCACCTTTTATTTGCAGATGATTCGATATTATTTTGTAAAGCCTCTGAAGGAGAAATGGACATGGTTAAGCACGTCCTTGATCTGTATGCAAAAGGCTCTGGGCAGCAAGTGAACTTTGACAAAAGTTCTCTCTTTTTTAGTACCAATTGTTCGCAAGAAACTCGTGATCGTTTGGCAAGAAGTTTAAATATTCCCCAGGGGCGGGGATTTGGGAAATACTTGGGACTCAAATTTGAATTTGGCCACTCCAAGAAGGAGGTGTTTGAAGAGATCAGGGAGAAGGTGGCGACACGGGTGCATGGATGGGCAGAGAATTTTCTAACCACGGCTGGAAATGAGGTGTTGTTGAAAGCGGTGGCACTTGCGCTACCCACGTATACTATGTTCTGCTTCCTACTTTCGCAGGGCGTGTGTGCGGATATCCTTCGTGCGCTCTCTTCTTTTTGGTGGGGTAGCCAAGGTACTGGCAAGGGGTTACACTGGGCAAAATGGAAGACGCTGACACAGAGGAAGGAATGGGGGCTGGATTTTAAGGACCTGTTTTGCTTTAACCGTGCTATGCTTGCCAAGATTGGTCGGCGCTTATTATCGCAGCCAAATTCGCTGCTTCATCAGTATTTTAAAAGGCCGGCAGGTGTTAATGCAGGGCTAAGATGGTGTATTGGAGACGGAAAGTCAGTCCATGTTACACGAGATCCGTGGTTGCCGATGCCCTACCACTTCAAGGTTAGGACACCATCACCTTTGCTGCCGGAAAAGGTATCGGATCTTATTGATCCTGTTTTGCGCCAATGGGATGTGCAGACGGTATGTCATGTATTTAACGAGGAGGCTGATTCCATCCTGGCCATGGCGCTTAGTCGATTTGGATGTCCGGATCATTTGATGTGGCATTTTACAAAAAATGGACTCTATACTGTAAAGTCGGGGTATCGAGTGGCAGTGGAGTTGGATTCCAATGGCCTCCTTGGACGACGAGGGGGTGGAAGAACTAGTGTTGATAGAGGAGGAAGACGGCTATGGAAATCAATCTGGGGACTGAACGTGGCGAGCAAGATTCGTCACTTCACTAAGGAGCTGGGGACGGAGACGACACCCGTTCATGGAGGTGGCGGAGAACATCGACTCATGGGCACTCAAAAATGGTCCTGCCCTCCGATGGCTTTTGCTAAAATCAATTGTGACGGGGCATGGACGTCACAAATTTTGCGTGGTGGGTGGGGTTGGGTGATGCGTGATGCAGCTGGGGTGTTCAAGGGGCCTGGGGTTGAAGGGGGTGTACGATGCGGCGCTGCAATAGTTGCTGAAGCCGAGGCATTACAGGCAGGGCTGTGTGCGGGGATGGACCAGGGCTGGCAACGGGTGGTGCTGGAGTCTGACTCCAAGCTCATGATTGATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

937

Amino Acids

105.71

Weight (kDa)

9.57

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2030
AatII GACGTC 1 cut(s) 2606
Acc16I TGCGCA 1 cut(s) 5
Acc36I ACCTGC 1 cut(s) 2030
AccB1I GGYRCC 1 cut(s) 398
AccI GTMKAC 2 cut(s) 100, 1784
AccII CGCG 4 cut(s) 120, 370, 559, 720
AccIII TCCGGA 1 cut(s) 2281
AclWI GGATC 6 cut(s) 199, 720, 2096, 2177, 2181, 2292
AcoI YGGCCR 4 cut(s) 24, 1203, 1648, 2253
AcsI RAATTY 7 cut(s) 16, 423, 1637, 1643, 1720, 2005, 2610
AcuI CTGAAG 2 cut(s) 1473, 2733
AcvI CACGTG 1 cut(s) 1127
AcyI GRCGYC 1 cut(s) 2603
AfaI GTAC 6 cut(s) 300, 1101, 1240, 1549, 1870, 2688
AfeI AGCGCT 1 cut(s) 1335
AflIII ACRYGT 1 cut(s) 1124
AgsI TTSAA 9 cut(s) 743, 791, 877, 1643, 1673, 1756, 2131, 2662, 2678
AhdI GACNNNNNGTC 3 cut(s) 1291, 2080, 2217
AhlI ACTAGT 1 cut(s) 2399
AjiI CACGTC 1 cut(s) 1480
AjnI CCWGG 7 cut(s) 137, 228, 803, 1602, 2250, 2668, 2754
AluBI AGCT 9 cut(s) 296, 661, 677, 839, 1172, 1363, 2485, 2651, 2794
AluI AGCT 9 cut(s) 296, 661, 677, 839, 1172, 1363, 2485, 2651, 2794
Alw26I GTCTC 4 cut(s) 1060, 1299, 2067, 2489
AlwI GGATC 6 cut(s) 199, 720, 2096, 2177, 2181, 2292
AlwNI CAGNNNCTG 2 cut(s) 191, 767
Ama87I CYCGRG 2 cut(s) 466, 750
Aor13HI TCCGGA 1 cut(s) 2281
Aor51HI AGCGCT 1 cut(s) 1335
ApoI RAATTY 7 cut(s) 16, 423, 1637, 1643, 1720, 2005, 2610
AseI ATTAAT 2 cut(s) 435, 885
AspS9I GGNCC 6 cut(s) 1191, 1285, 1942, 2553, 2666, 2752
AsuC2I CCSGG 2 cut(s) 467, 468
AsuHPI GGTGA 6 cut(s) 244, 530, 739, 1243, 2139, 2647
AvaI CYCGRG 2 cut(s) 466, 750
AvaII GGWCC 4 cut(s) 1285, 1942, 2553, 2752
BaeGI GKGCMC 1 cut(s) 2543
BalI TGGCCA 4 cut(s) 26, 1205, 1650, 2255
BanI GGYRCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 332
BarI GAAGNNNNNNTAC 2 cut(s) 2670, 2702
BauI CACGAG 2 cut(s) 1569, 2097
BbrPI CACGTG 1 cut(s) 1127
BbsI GAAGAC 2 cut(s) 1907, 2425
BccI CCATC 7 cut(s) 806, 1704, 2055, 2152, 2255, 2560, 2743
BceAI ACGGC 3 cut(s) 1062, 1749, 2438
BcgI CGANNNNNNTGC 6 cut(s) 1410, 1444, 1550, 1584, 1684, 1718
BciT130I CCWGG 7 cut(s) 139, 230, 805, 1604, 2252, 2670, 2756
BciVI GTATCC 1 cut(s) 621
BclI TGATCA 2 cut(s) 193, 259
BcnI CCSGG 2 cut(s) 467, 468
BcoDI GTCTC 4 cut(s) 1060, 1299, 2067, 2489
BcuI ACTAGT 1 cut(s) 2399
BfaI CTAG 2 cut(s) 497, 2400
BfmI CTRYAG 2 cut(s) 672, 840
BfoI RGCGCY 4 cut(s) 1337, 1990, 2264, 2700
BfuAI ACCTGC 1 cut(s) 2030
BfuI GTATCC 1 cut(s) 621
BglII AGATCT 1 cut(s) 505
BmcAI AGTACT 1 cut(s) 1101
Bme1390I CCNGG 9 cut(s) 139, 230, 467, 468, 805, 1604, 2252, 2670, 2756
Bme18I GGWCC 4 cut(s) 1285, 1942, 2553, 2752
BmeRI GACNNNNNGTC 3 cut(s) 1291, 2080, 2217
BmeT110I CYCGRG 2 cut(s) 466, 750
BmgBI CACGTC 1 cut(s) 1480
BmgT120I GGNCC 6 cut(s) 1191, 1285, 1942, 2553, 2666, 2752
BmiI GGNNCC 5 cut(s) 222, 400, 1286, 1287, 2667
BmrFI CCNGG 9 cut(s) 139, 230, 467, 468, 805, 1604, 2252, 2670, 2756
BmrI ACTGGG 2 cut(s) 831, 1897
BmsI GCATC 7 cut(s) 137, 154, 1200, 2106, 2628, 2635, 2681
BmuI ACTGGG 2 cut(s) 831, 1897
BpiI GAAGAC 2 cut(s) 1907, 2425
BplI GAGNNNNNCTC 4 cut(s) 913, 945, 2771, 2803
BpmI CTGGAG 2 cut(s) 668, 2798
BpuMI CCSGG 2 cut(s) 467, 468
BsaAI YACGTR 2 cut(s) 1127, 1782
BsaHI GRCGYC 1 cut(s) 2603
BsaWI WCCGGW 2 cut(s) 1058, 2281
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse118I RCCGGY 1 cut(s) 2035
Bse1I ACTGG 6 cut(s) 224, 351, 685, 826, 1876, 1892
Bse3DI GCAATG 2 cut(s) 392, 422
BseAI TCCGGA 1 cut(s) 2281
BseBI CCWGG 7 cut(s) 139, 230, 805, 1604, 2252, 2670, 2756
BseGI GGATG 8 cut(s) 131, 835, 1254, 1715, 2209, 2247, 2282, 2754
BseMI GCAATG 2 cut(s) 392, 422
BseNI ACTGG 6 cut(s) 224, 351, 685, 826, 1876, 1892
BseRI GAGGAG 4 cut(s) 406, 707, 2249, 2427
BseSI GKGCMC 1 cut(s) 2543
BseYI CCCAGC 2 cut(s) 2485, 2651
BsgI GTGCAG 1 cut(s) 2228
Bsh1236I CGCG 4 cut(s) 120, 370, 559, 720
Bsh1285I CGRYCG 1 cut(s) 1180
BshNI GGYRCC 1 cut(s) 398
BsiEI CGRYCG 1 cut(s) 1180
BsiHKCI CYCGRG 2 cut(s) 466, 750
BsiSI CCGG 5 cut(s) 467, 1059, 2036, 2159, 2282
BslFI GGGAC 4 cut(s) 1298, 1644, 2457, 2503
BsmAI GTCTC 4 cut(s) 1060, 1299, 2067, 2489
BsmBI CGTCTC 2 cut(s) 2067, 2489
BsmFI GGGAC 4 cut(s) 1298, 1644, 2457, 2503
BsmI GAATGC 1 cut(s) 851
BsoBI CYCGRG 2 cut(s) 466, 750
Bsp1286I GDGCHC 2 cut(s) 332, 2543
Bsp13I TCCGGA 1 cut(s) 2281
Bsp1407I TGTACA 1 cut(s) 298
Bsp19I CCATGG 1 cut(s) 2256
BspEI TCCGGA 1 cut(s) 2281
BspFNI CGCG 4 cut(s) 120, 370, 559, 720
BspHI TCATGA 1 cut(s) 2796
BspLI GGNNCC 5 cut(s) 222, 400, 1286, 1287, 2667
BspMAI CTGCAG 2 cut(s) 676, 844
BspMI ACCTGC 1 cut(s) 2030
BspPI GGATC 6 cut(s) 199, 720, 2096, 2177, 2181, 2292
BspQI GCTCTTC 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 398
BsrDI GCAATG 2 cut(s) 392, 422
BsrFI RCCGGY 1 cut(s) 2035
BsrGI TGTACA 1 cut(s) 298
BsrI ACTGG 6 cut(s) 224, 351, 685, 826, 1876, 1892
BssAI RCCGGY 1 cut(s) 2035
BssNAI GTATAC 2 cut(s) 101, 1785
BssNI GRCGYC 1 cut(s) 2603
BssSI CACGAG 2 cut(s) 1569, 2097
BssT1I CCWWGG 4 cut(s) 162, 1864, 2256, 2374
Bst1107I GTATAC 2 cut(s) 101, 1785
Bst2BI CACGAG 2 cut(s) 1569, 2097
Bst2UI CCWGG 7 cut(s) 139, 230, 805, 1604, 2252, 2670, 2756
Bst4CI ACNGT 5 cut(s) 553, 707, 1961, 2215, 2326
Bst6I CTCTTC 3 cut(s) 668, 1668, 1849
BstACI GRCGYC 1 cut(s) 2603
BstAPI GCANNNNNTGC 1 cut(s) 2708
BstAUI TGTACA 1 cut(s) 298
BstBAI YACGTR 2 cut(s) 1127, 1782
BstC8I GCNNGC 5 cut(s) 1274, 1971, 2037, 2458, 2762
BstDEI CTNAG 4 cut(s) 483, 2057, 2264, 2478
BstDSI CCRYGG 3 cut(s) 1731, 2105, 2256
BstEII GGTNACC 1 cut(s) 59
BstF5I GGATG 8 cut(s) 131, 835, 1254, 1715, 2209, 2247, 2282, 2754
BstFNI CGCG 4 cut(s) 120, 370, 559, 720
BstH2I RGCGCY 4 cut(s) 1337, 1990, 2264, 2700
BstMAI GTCTC 4 cut(s) 1060, 1299, 2067, 2489
BstMCI CGRYCG 1 cut(s) 1180
BstMWI GCNNNNNNNGC 6 cut(s) 117, 848, 1970, 2008, 2708, 2729
BstNI CCWGG 7 cut(s) 139, 230, 805, 1604, 2252, 2670, 2756
BstPI GGTNACC 1 cut(s) 59
BstSCI CCNGG 9 cut(s) 137, 228, 465, 466, 803, 1602, 2250, 2668, 2754
BstSFI CTRYAG 2 cut(s) 672, 840
BstSLI GKGCMC 1 cut(s) 2543
BstUI CGCG 4 cut(s) 120, 370, 559, 720
BstV2I GAAGAC 2 cut(s) 1907, 2425
BstX2I RGATCY 3 cut(s) 505, 2101, 2173
BstXI CCANNNNNNTGG 1 cut(s) 1871
BstYI RGATCY 3 cut(s) 505, 2101, 2173
BstZ17I GTATAC 2 cut(s) 101, 1785
BsuI GTATCC 1 cut(s) 621
BtgI CCRYGG 3 cut(s) 1731, 2105, 2256
BtgZI GCGATG 3 cut(s) 131, 622, 701
BtrI CACGTC 1 cut(s) 1480
BtsCI GGATG 8 cut(s) 131, 835, 1254, 1715, 2209, 2247, 2282, 2754
BtsI GCAGTG 1 cut(s) 2355
BtsIMutI CAGTG 3 cut(s) 819, 1885, 2355
BveI ACCTGC 1 cut(s) 2030
Cac8I GCNNGC 5 cut(s) 1274, 1971, 2037, 2458, 2762
CaiI CAGNNNCTG 2 cut(s) 191, 767
CciI TCATGA 1 cut(s) 2796
Cfr10I RCCGGY 1 cut(s) 2035
Cfr13I GGNCC 6 cut(s) 1191, 1285, 1942, 2553, 2666, 2752
Cfr9I CCCGGG 1 cut(s) 466
CseI GACGC 2 cut(s) 565, 1912
Csp6I GTAC 6 cut(s) 299, 1100, 1239, 1548, 1869, 2687
CviQI GTAC 6 cut(s) 299, 1100, 1239, 1548, 1869, 2687
DdeI CTNAG 4 cut(s) 483, 2057, 2264, 2478
DraI TTTAAA 2 cut(s) 1593, 2029
DriI GACNNNNNGTC 3 cut(s) 1291, 2080, 2217
EaeI YGGCCR 4 cut(s) 24, 1203, 1648, 2253
Eam1104I CTCTTC 3 cut(s) 668, 1668, 1849
Eam1105I GACNNNNNGTC 3 cut(s) 1291, 2080, 2217
EarI CTCTTC 3 cut(s) 668, 1668, 1849
EciI GGCGGA 1 cut(s) 2535
Eco130I CCWWGG 4 cut(s) 162, 1864, 2256, 2374
Eco24I GRGCYC 1 cut(s) 332
Eco32I GATATC 2 cut(s) 984, 1828
Eco47I GGWCC 4 cut(s) 1285, 1942, 2553, 2752
Eco47III AGCGCT 1 cut(s) 1335
Eco57I CTGAAG 2 cut(s) 1473, 2733
Eco72I CACGTG 1 cut(s) 1127
Eco88I CYCGRG 2 cut(s) 466, 750
Eco91I GGTNACC 1 cut(s) 59
EcoO109I RGGNCCY 3 cut(s) 1285, 1942, 2666
EcoO65I GGTNACC 1 cut(s) 59
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 7 cut(s) 137, 228, 803, 1602, 2250, 2668, 2754
EcoRV GATATC 2 cut(s) 984, 1828
EcoT14I CCWWGG 4 cut(s) 162, 1864, 2256, 2374
EcoT38I GRGCYC 1 cut(s) 332
ErhI CCWWGG 4 cut(s) 162, 1864, 2256, 2374
Esp3I CGTCTC 2 cut(s) 2067, 2489
FalI AAGNNNNNCTT 2 cut(s) 96, 128
FaqI GGGAC 4 cut(s) 1298, 1644, 2457, 2503
FauI CCCGC 5 cut(s) 26, 363, 1345, 1602, 2737
FbaI TGATCA 2 cut(s) 193, 259
FblI GTMKAC 2 cut(s) 100, 1784
FokI GGATG 8 cut(s) 118, 842, 1241, 1722, 2216, 2234, 2289, 2761
FriOI GRGCYC 1 cut(s) 332
FspBI CTAG 2 cut(s) 497, 2400
FspI TGCGCA 1 cut(s) 5
GsaI CCCAGC 2 cut(s) 2489, 2655
GsuI CTGGAG 2 cut(s) 668, 2798
HaeII RGCGCY 4 cut(s) 1337, 1990, 2264, 2700
HapII CCGG 5 cut(s) 467, 1059, 2036, 2159, 2282
HgaI GACGC 2 cut(s) 565, 1912
Hin1I GRCGYC 1 cut(s) 2603
HindIII AAGCTT 1 cut(s) 1170
HpaII CCGG 5 cut(s) 467, 1059, 2036, 2159, 2282
HphI GGTGA 6 cut(s) 244, 530, 739, 1243, 2139, 2647
Hpy166II GTNNAC 4 cut(s) 101, 1519, 1785, 2687
Hpy188III TCNNGA 6 cut(s) 428, 1084, 1309, 1571, 2282, 2797
Hpy8I GTNNAC 4 cut(s) 101, 1519, 1785, 2687
Hpy99I CGWCG 3 cut(s) 570, 1249, 2385
HpyAV CCTTC 8 cut(s) 616, 982, 1448, 1654, 1681, 1841, 1912, 2672
HpyCH4III ACNGT 5 cut(s) 553, 707, 1961, 2215, 2326
HpyCH4IV ACGT 5 cut(s) 1126, 1479, 1781, 2451, 2603
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 848, 1970, 2008, 2708, 2729
HpyF3I CTNAG 4 cut(s) 483, 2057, 2264, 2478
HpySE526I ACGT 5 cut(s) 1126, 1479, 1781, 2451, 2603
Hsp92I GRCGYC 1 cut(s) 2603
KflI GGGWCCC 1 cut(s) 1285
Kpn2I TCCGGA 1 cut(s) 2281
KroI GCCGGC 1 cut(s) 2035
KroNI GCCGGC 1 cut(s) 2037
Ksp22I TGATCA 2 cut(s) 193, 259
LguI GCTCTTC 1 cut(s) 668
LmnI GCTCC 4 cut(s) 75, 220, 1360, 2482
LweI GCATC 7 cut(s) 137, 154, 1200, 2106, 2628, 2635, 2681
MaeI CTAG 2 cut(s) 497, 2400
MaeII ACGT 5 cut(s) 1126, 1479, 1781, 2451, 2603
MaeIII GTNAC 9 cut(s) 59, 232, 553, 757, 1881, 2092, 2468, 2588, 2604
MfeI CAATTG 2 cut(s) 1552, 2584
MflI RGATCY 3 cut(s) 505, 2101, 2173
MhlI GDGCHC 2 cut(s) 332, 2543
MlsI TGGCCA 4 cut(s) 26, 1205, 1650, 2255
MluNI TGGCCA 4 cut(s) 26, 1205, 1650, 2255
MlyI GAGTC 7 cut(s) 931, 1145, 1626, 2310, 2526, 2780, 2789
MmeI TCCRAC 5 cut(s) 290, 824, 1113, 1137, 2337
Mox20I TGGCCA 4 cut(s) 26, 1205, 1650, 2255
MroI TCCGGA 1 cut(s) 2281
MroNI GCCGGC 1 cut(s) 2035
MscI TGGCCA 4 cut(s) 26, 1205, 1650, 2255
MslI CAYNNNNRTG 3 cut(s) 615, 894, 2514
Msp20I TGGCCA 4 cut(s) 26, 1205, 1650, 2255
MspA1I CMGCKG 2 cut(s) 296, 2651
MspI CCGG 5 cut(s) 467, 1059, 2036, 2159, 2282
MspR9I CCNGG 9 cut(s) 139, 230, 467, 468, 805, 1604, 2252, 2670, 2756
MunI CAATTG 2 cut(s) 1552, 2584
Mva1269I GAATGC 1 cut(s) 851
MvaI CCWGG 7 cut(s) 139, 230, 805, 1604, 2252, 2670, 2756
MvnI CGCG 4 cut(s) 120, 370, 559, 720
MwoI GCNNNNNNNGC 6 cut(s) 117, 848, 1970, 2008, 2708, 2729
NaeI GCCGGC 1 cut(s) 2037
NciI CCSGG 2 cut(s) 467, 468
NcoI CCATGG 1 cut(s) 2256
NgoMIV GCCGGC 1 cut(s) 2035
NlaIV GGNNCC 5 cut(s) 222, 400, 1286, 1287, 2667
NmeAIII GCCGAG 1 cut(s) 2743
NmuCI GTSAC 5 cut(s) 232, 553, 2468, 2588, 2604
NsbI TGCGCA 1 cut(s) 5
PagI TCATGA 1 cut(s) 2796
PaqCI CACCTGC 1 cut(s) 2030
PasI CCCWGGG 1 cut(s) 1603
PciSI GCTCTTC 1 cut(s) 668
PctI GAATGC 1 cut(s) 851
PdiI GCCGGC 1 cut(s) 2037
PfeI GAWTC 5 cut(s) 1039, 1427, 2243, 2360, 2464
PflFI GACNNNGTC 1 cut(s) 1079
Ple19I CGATCG 1 cut(s) 1180
PleI GAGTC 7 cut(s) 930, 1144, 1626, 2310, 2526, 2780, 2788
PmaCI CACGTG 1 cut(s) 1127
PmlI CACGTG 1 cut(s) 1127
PpsI GAGTC 7 cut(s) 930, 1144, 1626, 2310, 2526, 2780, 2788
Ppu21I YACGTR 2 cut(s) 1127, 1782
PpuMI RGGWCCY 2 cut(s) 1285, 1942
PshBI ATTAAT 2 cut(s) 435, 885
Psp5II RGGWCCY 2 cut(s) 1285, 1942
Psp6I CCWGG 7 cut(s) 137, 228, 803, 1602, 2250, 2668, 2754
PspCI CACGTG 1 cut(s) 1127
PspEI GGTNACC 1 cut(s) 59
PspFI CCCAGC 2 cut(s) 2485, 2651
PspGI CCWGG 7 cut(s) 137, 228, 803, 1602, 2250, 2668, 2754
PspN4I GGNNCC 5 cut(s) 222, 400, 1286, 1287, 2667
PspPI GGNCC 6 cut(s) 1191, 1285, 1942, 2553, 2666, 2752
PspPPI RGGWCCY 2 cut(s) 1285, 1942
PsrI GAACNNNNNNTAC 4 cut(s) 1540, 1572, 1775, 1807
PstI CTGCAG 2 cut(s) 676, 844
PstNI CAGNNNCTG 2 cut(s) 191, 767
PsuI RGATCY 3 cut(s) 505, 2101, 2173
PsyI GACNNNGTC 1 cut(s) 1079
PvuI CGATCG 1 cut(s) 1180
PvuII CAGCTG 2 cut(s) 296, 2651
RsaI GTAC 6 cut(s) 300, 1101, 1240, 1549, 1870, 2688
RsaNI GTAC 6 cut(s) 299, 1100, 1239, 1548, 1869, 2687
RseI CAYNNNNRTG 3 cut(s) 615, 894, 2514
SapI GCTCTTC 1 cut(s) 668
Sau96I GGNCC 6 cut(s) 1191, 1285, 1942, 2553, 2666, 2752
ScaI AGTACT 1 cut(s) 1101
SchI GAGTC 7 cut(s) 931, 1145, 1626, 2310, 2526, 2780, 2789
ScrFI CCNGG 9 cut(s) 139, 230, 467, 468, 805, 1604, 2252, 2670, 2756
SduI GDGCHC 2 cut(s) 332, 2543
SfaNI GCATC 7 cut(s) 137, 154, 1200, 2106, 2628, 2635, 2681
SfcI CTRYAG 2 cut(s) 672, 840
SinI GGWCC 4 cut(s) 1285, 1942, 2553, 2752
SmaI CCCGGG 1 cut(s) 468
SmiMI CAYNNNNRTG 3 cut(s) 615, 894, 2514
SpeI ACTAGT 1 cut(s) 2399
SspI AATATT 1 cut(s) 1597
SspMI CTAG 2 cut(s) 497, 2400
StyD4I CCNGG 9 cut(s) 137, 228, 465, 466, 803, 1602, 2250, 2668, 2754
StyI CCWWGG 4 cut(s) 162, 1864, 2256, 2374
TaaI ACNGT 5 cut(s) 553, 707, 1961, 2215, 2326
TaiI ACGT 5 cut(s) 1129, 1482, 1784, 2454, 2606
TaqI TCGA 8 cut(s) 42, 343, 931, 986, 1430, 2269, 2341, 2530
TaqII GACCGA 1 cut(s) 468
TatI WGTACW 2 cut(s) 298, 1099
TauI GCSGC 5 cut(s) 385, 562, 565, 1279, 2697
TfiI GAWTC 5 cut(s) 1039, 1427, 2243, 2360, 2464
TscAI CASTG 3 cut(s) 826, 1892, 2355
TseFI GTSAC 5 cut(s) 232, 553, 2468, 2588, 2604
Tsp45I GTSAC 5 cut(s) 232, 553, 2468, 2588, 2604
TspDTI ATGAA 5 cut(s) 530, 609, 1243, 2006, 2498
TspGWI ACGGA 4 cut(s) 1334, 2091, 2094, 2507
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 3 cut(s) 826, 1892, 2355
Tth111I GACNNNGTC 1 cut(s) 1079
VpaK11BI GGWCC 4 cut(s) 1285, 1942, 2553, 2752
VspI ATTAAT 2 cut(s) 435, 885
XapI RAATTY 7 cut(s) 16, 423, 1637, 1643, 1720, 2005, 2610
XcmI CCANNNNNNNNNTGG 1 cut(s) 2254
XmaI CCCGGG 1 cut(s) 466
XmiI GTMKAC 2 cut(s) 100, 1784
XspI CTAG 2 cut(s) 497, 2400
ZraI GACGTC 1 cut(s) 2604
ZrmI AGTACT 1 cut(s) 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.