pycom10g22280

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000194
Physical Location & Seq
Reverse (-)
24685242 .. 24686963
1722 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g22280.1

Sequence Viewer

Length: 1722 bp
ATGAGTATCTTCAAATTCCCCGCAGTTGTGTGTAATGAATTAGATGCGTTAGTGGCTGGTTTTTGGTGGGGATGTAAGGAGGGAGTCCATAAAATTCATTGGGTCTCAAAGGAGGTTCTGGGTATTCCTAAAGATCAGGGAGGTTTAGGCTTCAGAAATTTTCAGGAATTTAATGATGCTCTGCTTGCAAAGCAGTGCTGGAGATTAATTACTGAACCAGAATCGTTATGGGCTCAGGTCATTAAGGCTAGGTATTTTCCTCATTGCTCGATATGGGACGCGAAGAAGGGTGGTCGGGCGTCTTGGGCGTGGAGTAGCCTTCTTTGTGGCAGGGACTTGATTAGGGAGGGTTCTCATTGGCAGATTATGGGGGGTCAGGAGGTTCGGGTATGGCAGGACAGATGGCTGCCATCCTTGGCCCTTGGTCATCCGGTGCCGGTTGGTCCGGTCGCTGTTACTCCTGGCTTACGAGTTAGTGCACTTATTTGTCCGGAGGAGGGGCGGTGGAACCTTGATTTTTTGCAGGCCGTCATTTCGGGGGAGGATATGCAGGCTATTGAGGAGACGCCTCTGGGGGATCTAAGTCGTAAAGATCGTCTTGTCTGGGAGTTTAATAAGAATGGGTGTTACTCGGTTAAGTCGGGGTATAGATGGCTGCAAGATAGATCGATAGAGTTGAGGGATACTCGTCGGCCTTCTGTTCGCAGGGTTCCCAGGGATTTATGGAAAGGTATCTGGAAGTTGGAGGTGCCTCCTAAGTTGCGGCATTTCTTGTGGATCACGGTGCATGGGTGTCTTCCTACTCGGGATGCTCTATTCCGCCGGAGATCCTCTCAAACCTCTACTTGTCCTATCTGTGGACACCACGATGAAACCTTGGAGCATATGTTCCTCTCTTGTTCATGGGTGGAACCTATTTGGTTTGGAGGGGTGTTGGGTTACAAAGTGGATCGGCTTTCTCTCCCAACGTGGGTGGAATGGATTATGGCAGTCTTCTCTCCGAACATGTGCACCACGGGCGACACGAAATGGAGGCGATCTTACATTGTATTCACTTGTTGGTGTATTTGGAAGGCCCGCTGTGAGTTCGTGTTTAATGGGGTGCCCTTATGTCCTCCTAAAGTTTTGGCTGCGATCTCTGTGGCGGTGAGCTCCTTCTTTGGTTCAAAGGCAGCGGTTGGGTGTAGAAAAGTGCGCGAAGGGAGGAAGGAAATTTTGAATGTGCGATGGTGTGCTCCGGTCCATCCTTTTGTGAAGATTAACGTGGATGCTAGCTGGTCCAAAGCCTCAAATTTGGGTTTTGCTGGAGTGATTGCGCGGGGACAGGACAGAAGGATGGTGGCTGCTGCGAGGTACGCTATCAGAGCTCCTTCGGCGGCTGCTGCGGAGGCCACCGCGTTGATGCATGGGTGCCAGTTAGGTGCAGCATTGGGAGTCAGGTATGTCATCTTGGAATCTGACTCCCTTGAAGCTATAAAATGTCTCTCTAGTTCGTTGTCTAGGGGCAGTTGGGAGGCCTTTCCCGTGTTGGCTCGGGTTCAGCAATTGGGAAGGGAATTTCTGGACTGTCAATGGTCGTGGGTGCCTAGAGTAGCAAATAGTGTGGCGCATGAGTTAGCGTCGGTTGATTTTCCGGAGATGTGTGATGTTGTTTGGGTCGATAGACCCCCATCTTCGTTGGTCTTTGTACTGAATAATGATGGGCTACCTTGTCCTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

574

Amino Acids

64.69

Weight (kDa)

8.92

Isoelectric Point (pI)

46.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 210 - 307 7.6e-20 zinc-binding in reverse transcriptase
RVT_3 PF13456 421 - 541 8.4e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 5 cut(s) 433, 748, 1102, 1410, 1582
AccII CGCG 4 cut(s) 281, 1197, 1318, 1397
AccIII TCCGGA 2 cut(s) 490, 1633
AclWI GGATC 4 cut(s) 585, 785, 822, 957
AcsI RAATTY 7 cut(s) 14, 93, 157, 167, 1212, 1291, 1556
AcuI CTGAAG 1 cut(s) 136
AcyI GRCGYC 2 cut(s) 299, 566
AfaI GTAC 2 cut(s) 1355, 1689
AfiI CCNNNNNNNGG 3 cut(s) 497, 857, 970
AflIII ACRYGT 1 cut(s) 1005
AgsI TTSAA 4 cut(s) 13, 1167, 1219, 1469
AjnI CCWGG 2 cut(s) 460, 713
AloI GAACNNNNNNTCC 2 cut(s) 872, 904
AluBI AGCT 4 cut(s) 1152, 1275, 1367, 1472
AluI AGCT 4 cut(s) 1152, 1275, 1367, 1472
Alw21I GWGCWC 5 cut(s) 481, 1013, 1154, 1237, 1369
Alw26I GTCTC 3 cut(s) 109, 557, 1487
Alw44I GTGCAC 2 cut(s) 477, 1009
AlwI GGATC 4 cut(s) 585, 785, 822, 957
Ama87I CYCGRG 2 cut(s) 804, 1533
Aor13HI TCCGGA 2 cut(s) 490, 1633
AoxI GGCC 6 cut(s) 417, 525, 692, 1074, 1389, 1515
ApaLI GTGCAC 2 cut(s) 477, 1009
ApeKI GCWGC 9 cut(s) 406, 655, 1130, 1172, 1343, 1346, 1379, 1382, 1424
ApoI RAATTY 7 cut(s) 14, 93, 157, 167, 1212, 1291, 1556
AseI ATTAAT 1 cut(s) 206
AspLEI GCGC 3 cut(s) 1197, 1318, 1609
AspS9I GGNCC 5 cut(s) 418, 443, 1075, 1240, 1278
AsuHPI GGTGA 1 cut(s) 1159
AsuNHI GCTAGC 1 cut(s) 1271
AvaI CYCGRG 2 cut(s) 804, 1533
AvaII GGWCC 3 cut(s) 443, 1240, 1278
BaeGI GKGCMC 3 cut(s) 481, 1013, 1107
BanI GGYRCC 5 cut(s) 433, 748, 1102, 1410, 1582
BanII GRGCYC 3 cut(s) 235, 1154, 1369
BbsI GAAGAC 2 cut(s) 788, 985
Bbv12I GWGCWC 5 cut(s) 481, 1013, 1154, 1237, 1369
BbvI GCAGC 9 cut(s) 393, 642, 1117, 1184, 1330, 1333, 1366, 1369, 1436
BccI CCATC 8 cut(s) 396, 418, 645, 1221, 1251, 1330, 1678, 1694
BceAI ACGGC 1 cut(s) 512
BciT130I CCWGG 2 cut(s) 462, 715
BciVI GTATCC 1 cut(s) 676
BcoDI GTCTC 3 cut(s) 109, 557, 1487
BfaI CTAG 5 cut(s) 249, 1272, 1488, 1500, 1587
BfuI GTATCC 1 cut(s) 676
Bme1390I CCNGG 2 cut(s) 462, 715
Bme18I GGWCC 3 cut(s) 443, 1240, 1278
BmeT110I CYCGRG 2 cut(s) 804, 1533
BmgT120I GGNCC 5 cut(s) 418, 443, 1075, 1240, 1278
BmiI GGNNCC 8 cut(s) 435, 509, 711, 750, 912, 1104, 1412, 1584
BmrFI CCNGG 2 cut(s) 462, 715
BmsI GCATC 5 cut(s) 34, 166, 799, 1258, 1392
BmtI GCTAGC 1 cut(s) 1275
BpiI GAAGAC 2 cut(s) 788, 985
BplI GAGNNNNNCTC 4 cut(s) 670, 702, 817, 849
BpmI CTGGAG 2 cut(s) 220, 1326
Bpu10I CCTNAGC 1 cut(s) 234
Bsa29I ATCGAT 1 cut(s) 668
BsaHI GRCGYC 2 cut(s) 299, 566
BsaI GGTCTC 1 cut(s) 109
BsaJI CCNNGG 6 cut(s) 414, 421, 713, 714, 876, 1014
BsaWI WCCGGW 5 cut(s) 430, 445, 490, 1237, 1633
BsaXI ACNNNNNCTCC 4 cut(s) 488, 518, 872, 902
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 857, 970
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 1 cut(s) 1414
Bse3DI GCAATG 1 cut(s) 262
BseAI TCCGGA 2 cut(s) 490, 1633
BseBI CCWGG 2 cut(s) 462, 715
BseCI ATCGAT 1 cut(s) 668
BseDI CCNNGG 6 cut(s) 414, 421, 713, 714, 876, 1014
BseGI GGATG 7 cut(s) 77, 410, 427, 814, 1243, 1273, 1341
BseLI CCNNNNNNNGG 3 cut(s) 497, 857, 970
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 1 cut(s) 1414
BseRI GAGGAG 2 cut(s) 509, 575
BseSI GKGCMC 3 cut(s) 481, 1013, 1107
BseXI GCAGC 9 cut(s) 393, 642, 1117, 1184, 1330, 1333, 1366, 1369, 1436
BsgI GTGCAG 1 cut(s) 1443
Bsh1236I CGCG 4 cut(s) 281, 1197, 1318, 1397
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 6 cut(s) 419, 527, 694, 1076, 1391, 1517
BshNI GGYRCC 5 cut(s) 433, 748, 1102, 1410, 1582
BshVI ATCGAT 1 cut(s) 668
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 5 cut(s) 481, 1013, 1154, 1237, 1369
BsiHKCI CYCGRG 2 cut(s) 804, 1533
BsiSI CCGG 7 cut(s) 431, 437, 446, 491, 823, 1238, 1634
BslFI GGGAC 3 cut(s) 290, 347, 1335
BslI CCNNNNNNNGG 3 cut(s) 497, 857, 970
BsmAI GTCTC 3 cut(s) 109, 557, 1487
BsmBI CGTCTC 1 cut(s) 557
BsmFI GGGAC 3 cut(s) 290, 347, 1335
BsnI GGCC 6 cut(s) 419, 527, 694, 1076, 1391, 1517
Bso31I GGTCTC 1 cut(s) 109
BsoBI CYCGRG 2 cut(s) 804, 1533
Bsp1286I GDGCHC 7 cut(s) 235, 481, 1013, 1107, 1154, 1237, 1369
Bsp13I TCCGGA 2 cut(s) 490, 1633
Bsp143I GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
BspANI GGCC 6 cut(s) 419, 527, 694, 1076, 1391, 1517
BspCNI CTCAG 1 cut(s) 247
BspDI ATCGAT 1 cut(s) 668
BspEI TCCGGA 2 cut(s) 490, 1633
BspFNI CGCG 4 cut(s) 281, 1197, 1318, 1397
BspLI GGNNCC 8 cut(s) 435, 509, 711, 750, 912, 1104, 1412, 1584
BspOI GCTAGC 1 cut(s) 1275
BspPI GGATC 4 cut(s) 585, 785, 822, 957
BspT107I GGYRCC 5 cut(s) 433, 748, 1102, 1410, 1582
BspTNI GGTCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 262
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 1 cut(s) 1414
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 6 cut(s) 414, 421, 713, 714, 876, 1014
BssMI GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
BssNI GRCGYC 2 cut(s) 299, 566
BssT1I CCWWGG 3 cut(s) 414, 421, 876
Bst2UI CCWGG 2 cut(s) 462, 715
Bst4CI ACNGT 2 cut(s) 784, 1568
BstACI GRCGYC 2 cut(s) 299, 566
BstC8I GCNNGC 5 cut(s) 186, 525, 552, 1078, 1273
BstDEI CTNAG 3 cut(s) 234, 581, 756
BstDSI CCRYGG 1 cut(s) 1014
BstF5I GGATG 7 cut(s) 77, 410, 427, 814, 1243, 1273, 1341
BstFNI CGCG 4 cut(s) 281, 1197, 1318, 1397
BstHHI GCGC 3 cut(s) 1197, 1318, 1609
BstKTI GATC 9 cut(s) 136, 580, 595, 668, 780, 830, 952, 1040, 1137
BstMAI GTCTC 3 cut(s) 109, 557, 1487
BstMBI GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
BstMCI CGRYCG 1 cut(s) 450
BstNI CCWGG 2 cut(s) 462, 715
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 2 cut(s) 460, 713
BstSLI GKGCMC 3 cut(s) 481, 1013, 1107
BstUI CGCG 4 cut(s) 281, 1197, 1318, 1397
BstV1I GCAGC 9 cut(s) 393, 642, 1117, 1184, 1330, 1333, 1366, 1369, 1436
BstV2I GAAGAC 2 cut(s) 788, 985
BstX2I RGATCY 2 cut(s) 577, 827
BstYI RGATCY 2 cut(s) 577, 827
Bsu15I ATCGAT 1 cut(s) 668
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 6 cut(s) 419, 527, 694, 1076, 1391, 1517
BsuTUI ATCGAT 1 cut(s) 668
BtgI CCRYGG 1 cut(s) 1014
BtgZI GCGATG 1 cut(s) 1240
BtsCI GGATG 7 cut(s) 77, 410, 427, 814, 1243, 1273, 1341
BtsI GCAGTG 1 cut(s) 200
BtsIMutI CAGTG 1 cut(s) 200
Cac8I GCNNGC 5 cut(s) 186, 525, 552, 1078, 1273
CfoI GCGC 3 cut(s) 1197, 1318, 1609
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 5 cut(s) 418, 443, 1075, 1240, 1278
ClaI ATCGAT 1 cut(s) 668
CseI GACGC 4 cut(s) 287, 288, 574, 1608
Csp6I GTAC 2 cut(s) 1354, 1688
CspCI CAANNNNNGTGG 6 cut(s) 954, 989, 1559, 1584, 1594, 1619
CviAII CATG 5 cut(s) 788, 903, 1006, 1406, 1610
CviQI GTAC 2 cut(s) 1354, 1688
DdeI CTNAG 3 cut(s) 234, 581, 756
DpnI GATC 9 cut(s) 135, 579, 594, 667, 779, 829, 951, 1039, 1136
DpnII GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
EciI GGCGGA 1 cut(s) 809
Ecl136II GAGCTC 2 cut(s) 1152, 1367
Eco130I CCWWGG 3 cut(s) 414, 421, 876
Eco147I AGGCCT 1 cut(s) 1517
Eco24I GRGCYC 3 cut(s) 235, 1154, 1369
Eco31I GGTCTC 1 cut(s) 109
Eco47I GGWCC 3 cut(s) 443, 1240, 1278
Eco53kI GAGCTC 2 cut(s) 1152, 1367
Eco57I CTGAAG 1 cut(s) 136
Eco88I CYCGRG 2 cut(s) 804, 1533
EcoICRI GAGCTC 2 cut(s) 1152, 1367
EcoRII CCWGG 2 cut(s) 460, 713
EcoT14I CCWWGG 3 cut(s) 414, 421, 876
EcoT22I ATGCAT 1 cut(s) 1407
EcoT38I GRGCYC 3 cut(s) 235, 1154, 1369
ErhI CCWWGG 3 cut(s) 414, 421, 876
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 5 cut(s) 791, 906, 1009, 1409, 1613
FalI AAGNNNNNCTT 2 cut(s) 582, 614
FaqI GGGAC 3 cut(s) 290, 347, 1335
FatI CATG 5 cut(s) 787, 902, 1005, 1405, 1609
FauI CCCGC 3 cut(s) 28, 1085, 1311
FauNDI CATATG 1 cut(s) 885
FokI GGATG 7 cut(s) 84, 397, 414, 821, 1230, 1280, 1348
FriOI GRGCYC 3 cut(s) 235, 1154, 1369
FspBI CTAG 5 cut(s) 249, 1272, 1488, 1500, 1587
GlaI GCGC 3 cut(s) 1196, 1317, 1608
GsuI CTGGAG 2 cut(s) 220, 1326
HaeIII GGCC 6 cut(s) 419, 527, 694, 1076, 1391, 1517
HapII CCGG 7 cut(s) 431, 437, 446, 491, 823, 1238, 1634
HgaI GACGC 4 cut(s) 287, 288, 574, 1608
HhaI GCGC 3 cut(s) 1197, 1318, 1609
Hin1I GRCGYC 2 cut(s) 299, 566
Hin1II CATG 5 cut(s) 791, 906, 1009, 1409, 1613
Hin6I GCGC 3 cut(s) 1195, 1316, 1607
HinP1I GCGC 3 cut(s) 1195, 1316, 1607
HinfI GANTC 5 cut(s) 84, 221, 1434, 1454, 1460
HpaII CCGG 7 cut(s) 431, 437, 446, 491, 823, 1238, 1634
HphI GGTGA 1 cut(s) 1159
Hpy166II GTNNAC 3 cut(s) 479, 860, 1011
Hpy188I TCNGA 4 cut(s) 155, 1002, 1364, 1459
Hpy188III TCNNGA 7 cut(s) 164, 377, 491, 736, 806, 1562, 1634
Hpy8I GTNNAC 3 cut(s) 479, 860, 1011
Hpy99I CGWCG 2 cut(s) 693, 1624
HpyCH4III ACNGT 2 cut(s) 784, 1568
HpyCH4IV ACGT 2 cut(s) 968, 1263
HpyCH4V TGCA 9 cut(s) 188, 479, 523, 550, 658, 787, 1011, 1405, 1424
HpyF3I CTNAG 3 cut(s) 234, 581, 756
HpySE526I ACGT 2 cut(s) 968, 1263
Hsp92I GRCGYC 2 cut(s) 299, 566
Hsp92II CATG 5 cut(s) 791, 906, 1009, 1409, 1613
HspAI GCGC 3 cut(s) 1195, 1316, 1607
Kpn2I TCCGGA 2 cut(s) 490, 1633
Kzo9I GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
LmnI GCTCC 4 cut(s) 880, 1157, 1240, 1372
Lsp1109I GCAGC 9 cut(s) 393, 642, 1117, 1184, 1330, 1333, 1366, 1369, 1436
LweI GCATC 5 cut(s) 34, 166, 799, 1258, 1392
MaeI CTAG 5 cut(s) 249, 1272, 1488, 1500, 1587
MaeII ACGT 2 cut(s) 968, 1263
MaeIII GTNAC 3 cut(s) 454, 626, 938
MalI GATC 9 cut(s) 135, 579, 594, 667, 779, 829, 951, 1039, 1136
MboI GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
MboII GAAGA 5 cut(s) 295, 788, 985, 1267, 1665
MfeI CAATTG 1 cut(s) 1544
MflI RGATCY 2 cut(s) 577, 827
MhlI GDGCHC 7 cut(s) 235, 481, 1013, 1107, 1154, 1237, 1369
MlyI GAGTC 3 cut(s) 93, 1443, 1454
MmeI TCCRAC 1 cut(s) 723
Mph1103I ATGCAT 1 cut(s) 1407
MroI TCCGGA 2 cut(s) 490, 1633
MseI TTAA 8 cut(s) 171, 206, 243, 612, 636, 1095, 1260, 1720
MslI CAYNNNNRTG 1 cut(s) 867
MspA1I CMGCKG 2 cut(s) 1080, 1175
MspI CCGG 7 cut(s) 431, 437, 446, 491, 823, 1238, 1634
MspR9I CCNGG 2 cut(s) 462, 715
MunI CAATTG 1 cut(s) 1544
MvaI CCWGG 2 cut(s) 462, 715
MvnI CGCG 4 cut(s) 281, 1197, 1318, 1397
NdeI CATATG 1 cut(s) 885
NdeII GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
NheI GCTAGC 1 cut(s) 1271
NlaIII CATG 5 cut(s) 791, 906, 1009, 1409, 1613
NlaIV GGNNCC 8 cut(s) 435, 509, 711, 750, 912, 1104, 1412, 1584
NsiI ATGCAT 1 cut(s) 1407
NspI RCATGY 1 cut(s) 1009
PasI CCCWGGG 1 cut(s) 714
PceI AGGCCT 1 cut(s) 1517
PciI ACATGT 1 cut(s) 1005
PcsI WCGNNNNNNNCGW 2 cut(s) 592, 1022
PfeI GAWTC 2 cut(s) 221, 1454
PleI GAGTC 3 cut(s) 92, 1442, 1454
PpsI GAGTC 3 cut(s) 92, 1442, 1454
PscI ACATGT 1 cut(s) 1005
PshBI ATTAAT 1 cut(s) 206
Psp124BI GAGCTC 2 cut(s) 1154, 1369
Psp6I CCWGG 2 cut(s) 460, 713
PspGI CCWGG 2 cut(s) 460, 713
PspN4I GGNNCC 8 cut(s) 435, 509, 711, 750, 912, 1104, 1412, 1584
PspPI GGNCC 5 cut(s) 418, 443, 1075, 1240, 1278
PsuI RGATCY 2 cut(s) 577, 827
RsaI GTAC 2 cut(s) 1355, 1689
RsaNI GTAC 2 cut(s) 1354, 1688
RseI CAYNNNNRTG 1 cut(s) 867
SacI GAGCTC 2 cut(s) 1154, 1369
SaqAI TTAA 8 cut(s) 171, 206, 243, 612, 636, 1095, 1260, 1720
Sau3AI GATC 9 cut(s) 133, 577, 592, 665, 777, 827, 949, 1037, 1134
Sau96I GGNCC 5 cut(s) 418, 443, 1075, 1240, 1278
SchI GAGTC 3 cut(s) 93, 1443, 1454
ScrFI CCNGG 2 cut(s) 462, 715
SduI GDGCHC 7 cut(s) 235, 481, 1013, 1107, 1154, 1237, 1369
SfaNI GCATC 5 cut(s) 34, 166, 799, 1258, 1392
SinI GGWCC 3 cut(s) 443, 1240, 1278
SmiMI CAYNNNNRTG 1 cut(s) 867
SseBI AGGCCT 1 cut(s) 1517
SspMI CTAG 5 cut(s) 249, 1272, 1488, 1500, 1587
SstI GAGCTC 2 cut(s) 1154, 1369
StuI AGGCCT 1 cut(s) 1517
StyD4I CCNGG 2 cut(s) 460, 713
StyI CCWWGG 3 cut(s) 414, 421, 876
TaaI ACNGT 2 cut(s) 784, 1568
TaiI ACGT 2 cut(s) 971, 1266
TaqI TCGA 3 cut(s) 269, 668, 1659
TatI WGTACW 1 cut(s) 1687
TauI GCSGC 2 cut(s) 766, 1379
TfiI GAWTC 2 cut(s) 221, 1454
Tru1I TTAA 8 cut(s) 171, 206, 243, 612, 636, 1095, 1260, 1720
Tru9I TTAA 8 cut(s) 171, 206, 243, 612, 636, 1095, 1260, 1720
TscAI CASTG 1 cut(s) 200
TseI GCWGC 9 cut(s) 406, 655, 1130, 1172, 1343, 1346, 1379, 1382, 1424
TspDTI ATGAA 4 cut(s) 51, 86, 885, 891
TspRI CASTG 1 cut(s) 200
VneI GTGCAC 2 cut(s) 477, 1009
VpaK11BI GGWCC 3 cut(s) 443, 1240, 1278
VspI ATTAAT 1 cut(s) 206
XapI RAATTY 7 cut(s) 14, 93, 157, 167, 1212, 1291, 1556
XceI RCATGY 1 cut(s) 1009
XcmI CCANNNNNNNNNTGG 1 cut(s) 225
XspI CTAG 5 cut(s) 249, 1272, 1488, 1500, 1587
Zsp2I ATGCAT 1 cut(s) 1407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.