Rmu_sc0002754.1_g000022

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002754.1
OG0000194
Physical Location & Seq
Forward (+)
92406 .. 94139
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002754.1_g000022.1.cds

Sequence Viewer

Length: 1065 bp
atgaattgttttcgttttccaaagacattgtgctctcggattaactcggacattgccaacttctggtggggaaagaaaggtgcaaatggaattcactggaaaaactggaaatttcttggtcttccaaaatctcaaggtggaatggggttccgagacctagaggattttaatcaggccttattagcgaagcaagggtggaggattctaatgaatcctgagtctctttgggtcagaatgttgaaagctaagtactttcctgatgtgcctttcttggaagctaggattggatcttctccttcttggttatggtcaagtttattacatgggcagcagctctcaaatctaatgttctttggagggtgggaaatggtttacatgttacaatatggaatactaattggattctcgcttgtaggacatacttctaaagctcaacaagcagccatccgggttattcctttaatcagctcatccattccggatactcttgtctggcctctcaacaaatccgggatttacatggttaaggcaggtactccagattctagattggttgcatctacagctttttatgcctctctagaattcttgtcctcaaagaagaacatagatactcaatcagtcaaatcctctctacgatccagaccaccaccaaaaccaaaatggattcctcctctgcctcaacaactgaagataaatagtgatgcttcttggacttctcagtctgtaggtgtggctactatcgctagagatagtacaggacacgtggtggggggtgttcactcattcagttcagccctaactcctctatatgctgaagctttagcccttttgacagctactaaccttgctcatagccttattgatcagcatattcaagtggttgctctcccttctaatagctggacttggtctagcaggttggctaatcaagcggcagatcacttagcttgtttggctcgcagaagaatgtgtccggatgattggtggaatcatcctccttcctccttatcttacttccttctcttagatgctgtttctactccagattccattttagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

354

Amino Acids

39.41

Weight (kDa)

9.65

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 721
Acc36I ACCTGC 2 cut(s) 521, 909
AccB7I CCANNNNNTGG 1 cut(s) 63
AccIII TCCGGA 2 cut(s) 478, 976
AciI CCGC 1 cut(s) 935
AclWI GGATC 2 cut(s) 295, 633
AcsI RAATTY 3 cut(s) 90, 110, 584
AcuI CTGAAG 2 cut(s) 710, 837
AcvI CACGTG 1 cut(s) 766
AdeI CACNNNGTG 1 cut(s) 769
AfaI GTAC 3 cut(s) 251, 535, 757
AfiI CCNNNNNNNGG 1 cut(s) 63
AflIII ACRYGT 2 cut(s) 375, 763
AgsI TTSAA 2 cut(s) 241, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 267, 299
Alw21I GWGCWC 1 cut(s) 35
Alw26I GTCTC 2 cut(s) 147, 225
AlwI GGATC 2 cut(s) 295, 633
Aor13HI TCCGGA 2 cut(s) 478, 976
AoxI GGCC 2 cut(s) 174, 494
ApeKI GCWGC 3 cut(s) 328, 331, 440
ApoI RAATTY 3 cut(s) 90, 110, 584
AsuC2I CCSGG 2 cut(s) 449, 511
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BbrPI CACGTG 1 cut(s) 766
BbsI GAAGAC 1 cut(s) 113
Bbv12I GWGCWC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 340, 343, 452
BccI CCATC 1 cut(s) 452
BciVI GTATCC 1 cut(s) 475
BclI TGATCA 1 cut(s) 865
BcnI CCSGG 2 cut(s) 449, 511
BcoDI GTCTC 2 cut(s) 147, 225
BfaI CTAG 6 cut(s) 158, 279, 546, 581, 747, 915
BfmI CTRYAG 2 cut(s) 561, 726
BfuAI ACCTGC 2 cut(s) 521, 909
BfuI GTATCC 1 cut(s) 475
BisI GCNGC 4 cut(s) 329, 332, 441, 936
BlsI GCNGC 4 cut(s) 330, 333, 442, 937
BmcAI AGTACT 1 cut(s) 251
Bme1390I CCNGG 2 cut(s) 449, 511
BmiI GGNNCC 1 cut(s) 149
BmrFI CCNGG 2 cut(s) 449, 511
BmsI GCATC 3 cut(s) 566, 694, 1021
BpiI GAAGAC 1 cut(s) 113
BpmI CTGGAG 2 cut(s) 522, 1029
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 2 cut(s) 449, 511
BsaAI YACGTR 1 cut(s) 766
BsaBI GATNNNNATC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 147
BsaWI WCCGGW 2 cut(s) 478, 976
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 2 cut(s) 101, 110
Bse3DI GCAATG 1 cut(s) 51
Bse8I GATNNNNATC 1 cut(s) 168
BseAI TCCGGA 2 cut(s) 478, 976
BseGI GGATG 4 cut(s) 444, 470, 985, 994
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 2 cut(s) 207, 734
BseNI ACTGG 2 cut(s) 101, 110
BseRI GAGGAG 2 cut(s) 663, 795
BseXI GCAGC 3 cut(s) 340, 343, 452
BshFI GGCC 2 cut(s) 176, 496
BsiHKAI GWGCWC 1 cut(s) 35
BsiSI CCGG 4 cut(s) 448, 479, 510, 977
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 2 cut(s) 147, 225
BsnI GGCC 2 cut(s) 176, 496
Bso31I GGTCTC 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 35
Bsp13I TCCGGA 2 cut(s) 478, 976
Bsp143I GATC 4 cut(s) 287, 638, 865, 940
BspACI CCGC 1 cut(s) 935
BspANI GGCC 2 cut(s) 176, 496
BspCNI CTCAG 2 cut(s) 208, 733
BspEI TCCGGA 2 cut(s) 478, 976
BspLI GGNNCC 1 cut(s) 149
BspMI ACCTGC 2 cut(s) 521, 909
BspPI GGATC 2 cut(s) 295, 633
BspTNI GGTCTC 1 cut(s) 147
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 2 cut(s) 101, 110
BssMI GATC 4 cut(s) 287, 638, 865, 940
BstBAI YACGTR 1 cut(s) 766
BstC8I GCNNGC 1 cut(s) 961
BstDEI CTNAG 5 cut(s) 216, 246, 720, 946, 1027
BstF5I GGATG 4 cut(s) 444, 470, 985, 994
BstKTI GATC 4 cut(s) 290, 641, 868, 943
BstMAI GTCTC 2 cut(s) 147, 225
BstMBI GATC 4 cut(s) 287, 638, 865, 940
BstMWI GCNNNNNNNGC 7 cut(s) 182, 437, 563, 572, 743, 932, 956
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 2 cut(s) 447, 509
BstSFI CTRYAG 2 cut(s) 561, 726
BstV1I GCAGC 3 cut(s) 340, 343, 452
BstV2I GAAGAC 1 cut(s) 113
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BsuI GTATCC 1 cut(s) 475
BsuRI GGCC 2 cut(s) 176, 496
BtsCI GGATG 4 cut(s) 444, 470, 985, 994
BtsIMutI CAGTG 1 cut(s) 94
BveI ACCTGC 2 cut(s) 521, 909
Cac8I GCNNGC 1 cut(s) 961
Csp6I GTAC 3 cut(s) 250, 534, 756
CviAII CATG 3 cut(s) 323, 376, 520
CviQI GTAC 3 cut(s) 250, 534, 756
DdeI CTNAG 5 cut(s) 216, 246, 720, 946, 1027
DpnI GATC 4 cut(s) 289, 640, 867, 942
DpnII GATC 4 cut(s) 287, 638, 865, 940
DraIII CACNNNGTG 1 cut(s) 769
DrdI GACNNNNNNGTC 1 cut(s) 721
DseDI GACNNNNNNGTC 1 cut(s) 721
Eco147I AGGCCT 1 cut(s) 176
Eco31I GGTCTC 1 cut(s) 147
Eco57I CTGAAG 2 cut(s) 710, 837
Eco72I CACGTG 1 cut(s) 766
EcoRI GAATTC 2 cut(s) 90, 584
FaeI CATG 3 cut(s) 326, 379, 523
FatI CATG 3 cut(s) 322, 375, 519
FbaI TGATCA 1 cut(s) 865
Fnu4HI GCNGC 4 cut(s) 329, 332, 441, 936
FokI GGATG 4 cut(s) 431, 457, 981, 992
Fsp4HI GCNGC 4 cut(s) 329, 332, 441, 936
FspBI CTAG 6 cut(s) 158, 279, 546, 581, 747, 915
GluI GCNGC 4 cut(s) 329, 332, 441, 936
GsuI CTGGAG 2 cut(s) 522, 1029
HaeIII GGCC 2 cut(s) 176, 496
HapII CCGG 4 cut(s) 448, 479, 510, 977
Hin1II CATG 3 cut(s) 326, 379, 523
HindIII AAGCTT 1 cut(s) 819
HinfI GANTC 8 cut(s) 202, 211, 218, 402, 542, 667, 991, 1049
HpaII CCGG 4 cut(s) 448, 479, 510, 977
Hpy166II GTNNAC 2 cut(s) 373, 781
Hpy188I TCNGA 4 cut(s) 39, 49, 152, 233
Hpy188III TCNNGA 9 cut(s) 215, 257, 479, 539, 546, 581, 642, 977, 1046
Hpy8I GTNNAC 2 cut(s) 373, 781
HpyAV CCTTC 4 cut(s) 306, 903, 1011, 1031
HpyCH4IV ACGT 1 cut(s) 765
HpyCH4V TGCA 2 cut(s) 83, 557
HpyF10VI GCNNNNNNNGC 7 cut(s) 182, 437, 563, 572, 743, 932, 956
HpyF3I CTNAG 5 cut(s) 216, 246, 720, 946, 1027
HpySE526I ACGT 1 cut(s) 765
Hsp92II CATG 3 cut(s) 326, 379, 523
Kpn2I TCCGGA 2 cut(s) 478, 976
Ksp22I TGATCA 1 cut(s) 865
Kzo9I GATC 4 cut(s) 287, 638, 865, 940
Lsp1109I GCAGC 3 cut(s) 340, 343, 452
LweI GCATC 3 cut(s) 566, 694, 1021
MaeI CTAG 6 cut(s) 158, 279, 546, 581, 747, 915
MaeII ACGT 1 cut(s) 765
MaeIII GTNAC 1 cut(s) 378
MalI GATC 4 cut(s) 289, 640, 867, 942
MboI GATC 4 cut(s) 287, 638, 865, 940
MboII GAAGA 5 cut(s) 113, 282, 613, 703, 978
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 5 cut(s) 4, 90, 110, 396, 584
MlyI GAGTC 1 cut(s) 227
MroI TCCGGA 2 cut(s) 478, 976
MseI TTAA 4 cut(s) 42, 168, 461, 525
MspI CCGG 4 cut(s) 448, 479, 510, 977
MspR9I CCNGG 2 cut(s) 449, 511
MwoI GCNNNNNNNGC 7 cut(s) 182, 437, 563, 572, 743, 932, 956
NciI CCSGG 2 cut(s) 449, 511
NdeII GATC 4 cut(s) 287, 638, 865, 940
NlaIII CATG 3 cut(s) 326, 379, 523
NlaIV GGNNCC 1 cut(s) 149
NspI RCATGY 1 cut(s) 379
PceI AGGCCT 1 cut(s) 176
PciI ACATGT 1 cut(s) 375
PfeI GAWTC 7 cut(s) 202, 211, 402, 542, 667, 991, 1049
PflFI GACNNNGTC 1 cut(s) 910
PflMI CCANNNNNTGG 1 cut(s) 63
PfoI TCCNGGA 1 cut(s) 509
PkrI GCNGC 4 cut(s) 330, 333, 442, 937
PleI GAGTC 1 cut(s) 226
PmaCI CACGTG 1 cut(s) 766
PmlI CACGTG 1 cut(s) 766
PpsI GAGTC 1 cut(s) 226
Ppu21I YACGTR 1 cut(s) 766
PscI ACATGT 1 cut(s) 375
PspCI CACGTG 1 cut(s) 766
PspN4I GGNNCC 1 cut(s) 149
PsuI RGATCY 1 cut(s) 287
PsyI GACNNNGTC 1 cut(s) 910
RsaI GTAC 3 cut(s) 251, 535, 757
RsaNI GTAC 3 cut(s) 250, 534, 756
SaqAI TTAA 4 cut(s) 42, 168, 461, 525
SatI GCNGC 4 cut(s) 329, 332, 441, 936
Sau3AI GATC 4 cut(s) 287, 638, 865, 940
ScaI AGTACT 1 cut(s) 251
SchI GAGTC 1 cut(s) 227
ScrFI CCNGG 2 cut(s) 449, 511
SduI GDGCHC 1 cut(s) 35
SfaNI GCATC 3 cut(s) 566, 694, 1021
SfcI CTRYAG 2 cut(s) 561, 726
SmlI CTYRAG 1 cut(s) 132
SmoI CTYRAG 1 cut(s) 132
Sse9I AATT 5 cut(s) 4, 90, 110, 396, 584
SseBI AGGCCT 1 cut(s) 176
SsiI CCGC 1 cut(s) 935
SspMI CTAG 6 cut(s) 158, 279, 546, 581, 747, 915
StuI AGGCCT 1 cut(s) 176
StyD4I CCNGG 2 cut(s) 447, 509
TaiI ACGT 1 cut(s) 768
TasI AATT 5 cut(s) 4, 90, 110, 396, 584
TatI WGTACW 2 cut(s) 249, 755
TauI GCSGC 1 cut(s) 938
TfiI GAWTC 7 cut(s) 202, 211, 402, 542, 667, 991, 1049
Tru1I TTAA 4 cut(s) 42, 168, 461, 525
Tru9I TTAA 4 cut(s) 42, 168, 461, 525
TscAI CASTG 1 cut(s) 101
TseI GCWGC 3 cut(s) 328, 331, 440
TspDTI ATGAA 2 cut(s) 17, 224
TspRI CASTG 1 cut(s) 101
Tth111I GACNNNGTC 1 cut(s) 910
Van91I CCANNNNNTGG 1 cut(s) 63
XapI RAATTY 3 cut(s) 90, 110, 584
XbaI TCTAGA 2 cut(s) 545, 580
XceI RCATGY 1 cut(s) 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 660
XspI CTAG 6 cut(s) 158, 279, 546, 581, 747, 915
ZrmI AGTACT 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.