Rorug04G0256500

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000194
Physical Location & Seq
Forward (+)
42906100 .. 42907663
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0256500.1

Sequence Viewer

Length: 879 bp
ATGGCTTCTGTATTGTCTGCTCAAGAAGCCTCACAGCTACTCAAATGTCAGACATGGATCTTGAAAGTGTTTATCCACTGTGAAGGTTGCAAGAGGAAAGTGAAGAAAGTTCTACTGGGAATTGATGGTGTTTACACAACATCTATTGAGGCAGAGCAAAACAGAGTCACAGTCACTGGAGACATTGACGTTCAGACCCTCATCAAGAAGCTGATTGTAAAAACTGGTAAACATGCCGAGCTATGGCCAGAAAATCTCACCAAGAAAGAAAAGACCAAGCCTGTGAAAGCCAAGAACAACGACAAACAGAAATCCTCAGAAATTTGCCAAAGTCAGCAGAGTACTACATGTGACAAAGTTGGAGTGGCCGGGCTAAACAGAGCAAGTGAGGGTGACACAGCTAAGTTTAACAGAGAGGAACAATGTCAAGTATCAAAGAAGGGTCACATTCCGCCGGAGAAATCTCCTGATGGCCATGATTCTCAGGCCATGGATTTTCAGCTCGGTGAAAGTGAAAAAGGGAGTGTTAGTGGCAAAAAGAACAGGAAAGGCCAAAGAGATGATTTGGGTTCAACAAGCTCCTGTGGACCTGCAAGCATAGGATCTCAACATTATGGTGATCACACTGCTGGTCCAGTTGATCTCAGCCATATCATCGCAAACATGTATCCGATGGATCTGGTGTTCAATGGAAGTGACATTACAGCAGCTGTTACAGCACTGCCTAACAAGAGCTTGGCTCCTTACTATTGTTATGGTCCATCATCACAGTACAAGGATGCAGCTAGTGCTGAACAGATTGATCAAGTGAAAGCAACATTTCTTAACAGTGTGCAGATTTTCAGTGATGAAAATGCTAATGGCTGTTTTATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

292

Amino Acids

31.72

Weight (kDa)

7.99

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 22 - 76 7.6e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 598
AciI CCGC 1 cut(s) 452
AclWI GGATC 3 cut(s) 65, 610, 684
AcoI YGGCCR 3 cut(s) 245, 366, 472
AcsI RAATTY 1 cut(s) 321
AfaI GTAC 2 cut(s) 343, 773
AfiI CCNNNNNNNGG 1 cut(s) 243
AflIII ACRYGT 2 cut(s) 347, 663
AgsI TTSAA 3 cut(s) 64, 573, 688
AloI GAACNNNNNNTCC 2 cut(s) 668, 700
AluBI AGCT 9 cut(s) 37, 211, 241, 401, 502, 579, 710, 735, 785
AluI AGCT 9 cut(s) 37, 211, 241, 401, 502, 579, 710, 735, 785
Alw26I GTCTC 1 cut(s) 174
AlwI GGATC 3 cut(s) 65, 610, 684
AlwNI CAGNNNCTG 2 cut(s) 176, 710
AoxI GGCC 5 cut(s) 245, 366, 472, 486, 550
ApeKI GCWGC 2 cut(s) 707, 782
ApoI RAATTY 1 cut(s) 321
AspS9I GGNCC 3 cut(s) 587, 632, 758
AsuC2I CCSGG 1 cut(s) 370
AsuHPI GGTGA 4 cut(s) 250, 404, 518, 629
AvaII GGWCC 3 cut(s) 587, 632, 758
BalI TGGCCA 2 cut(s) 247, 474
BarI GAAGNNNNNNTAC 2 cut(s) 685, 717
BbvI GCAGC 2 cut(s) 719, 794
BccI CCATC 4 cut(s) 119, 464, 667, 769
BciVI GTATCC 1 cut(s) 678
BclI TGATCA 2 cut(s) 619, 802
BcnI CCSGG 1 cut(s) 370
BcoDI GTCTC 1 cut(s) 174
BfaI CTAG 1 cut(s) 786
BfuAI ACCTGC 1 cut(s) 598
BfuI GTATCC 1 cut(s) 678
BisI GCNGC 2 cut(s) 708, 783
BlsI GCNGC 2 cut(s) 709, 784
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 370
Bme18I GGWCC 3 cut(s) 587, 632, 758
BmgT120I GGNCC 3 cut(s) 587, 632, 758
BmiI GGNNCC 1 cut(s) 741
BmrFI CCNGG 1 cut(s) 370
BmrI ACTGGG 1 cut(s) 125
BmsI GCATC 1 cut(s) 769
BmuI ACTGGG 1 cut(s) 125
BplI GAGNNNNNCTC 2 cut(s) 724, 756
BpmI CTGGAG 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 1 cut(s) 370
BsaJI CCNNGG 1 cut(s) 489
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 1 cut(s) 243
Bse1I ACTGG 4 cut(s) 120, 181, 229, 635
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 1 cut(s) 784
BseLI CCNNNNNNNGG 1 cut(s) 243
BseMII CTCAG 3 cut(s) 330, 497, 658
BseNI ACTGG 4 cut(s) 120, 181, 229, 635
BseXI GCAGC 2 cut(s) 719, 794
BsgI GTGCAG 1 cut(s) 854
BshFI GGCC 5 cut(s) 247, 368, 474, 488, 552
BsiSI CCGG 2 cut(s) 369, 455
BslI CCNNNNNNNGG 1 cut(s) 243
BsmAI GTCTC 1 cut(s) 174
BsnI GGCC 5 cut(s) 247, 368, 474, 488, 552
Bsp143I GATC 6 cut(s) 57, 602, 619, 640, 676, 802
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 1 cut(s) 452
BspANI GGCC 5 cut(s) 247, 368, 474, 488, 552
BspCNI CTCAG 3 cut(s) 329, 496, 657
BspLI GGNNCC 1 cut(s) 741
BspMI ACCTGC 1 cut(s) 598
BspPI GGATC 3 cut(s) 65, 610, 684
BsrI ACTGG 4 cut(s) 120, 181, 229, 635
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 6 cut(s) 57, 602, 619, 640, 676, 802
BssT1I CCWWGG 1 cut(s) 489
Bst4CI ACNGT 4 cut(s) 80, 172, 771, 830
BstAPI GCANNNNNTGC 1 cut(s) 788
BstC8I GCNNGC 1 cut(s) 595
BstDEI CTNAG 4 cut(s) 316, 402, 483, 644
BstDSI CCRYGG 1 cut(s) 489
BstF5I GGATG 1 cut(s) 784
BstKTI GATC 6 cut(s) 60, 605, 622, 643, 679, 805
BstMAI GTCTC 1 cut(s) 174
BstMBI GATC 6 cut(s) 57, 602, 619, 640, 676, 802
BstMWI GCNNNNNNNGC 3 cut(s) 26, 716, 788
BstNSI RCATGY 3 cut(s) 236, 351, 667
BstSCI CCNGG 1 cut(s) 368
BstV1I GCAGC 2 cut(s) 719, 794
BstX2I RGATCY 3 cut(s) 57, 602, 676
BstYI RGATCY 3 cut(s) 57, 602, 676
BsuI GTATCC 1 cut(s) 678
BsuRI GGCC 5 cut(s) 247, 368, 474, 488, 552
BtgI CCRYGG 1 cut(s) 489
BtgZI GCGATG 1 cut(s) 640
BtsCI GGATG 1 cut(s) 784
BtsI GCAGTG 2 cut(s) 624, 719
BtsIMutI CAGTG 6 cut(s) 76, 174, 624, 719, 835, 850
BveI ACCTGC 1 cut(s) 598
Cac8I GCNNGC 1 cut(s) 595
CaiI CAGNNNCTG 2 cut(s) 176, 710
Cfr13I GGNCC 3 cut(s) 587, 632, 758
Csp6I GTAC 2 cut(s) 342, 772
CviAII CATG 6 cut(s) 54, 233, 348, 476, 490, 664
CviQI GTAC 2 cut(s) 342, 772
DdeI CTNAG 4 cut(s) 316, 402, 483, 644
DpnI GATC 6 cut(s) 59, 604, 621, 642, 678, 804
DpnII GATC 6 cut(s) 57, 602, 619, 640, 676, 802
EaeI YGGCCR 3 cut(s) 245, 366, 472
EciI GGCGGA 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 489
Eco47I GGWCC 3 cut(s) 587, 632, 758
EcoT14I CCWWGG 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 6 cut(s) 57, 236, 351, 479, 493, 667
FatI CATG 6 cut(s) 53, 232, 347, 475, 489, 663
FbaI TGATCA 2 cut(s) 619, 802
Fnu4HI GCNGC 2 cut(s) 708, 783
FokI GGATG 1 cut(s) 791
Fsp4HI GCNGC 2 cut(s) 708, 783
FspBI CTAG 1 cut(s) 786
GluI GCNGC 2 cut(s) 708, 783
GsuI CTGGAG 1 cut(s) 198
HaeIII GGCC 5 cut(s) 247, 368, 474, 488, 552
HapII CCGG 2 cut(s) 369, 455
Hin1II CATG 6 cut(s) 57, 236, 351, 479, 493, 667
HinfI GANTC 2 cut(s) 165, 479
HpaII CCGG 2 cut(s) 369, 455
HphI GGTGA 4 cut(s) 250, 404, 518, 629
Hpy166II GTNNAC 3 cut(s) 133, 230, 587
Hpy188I TCNGA 4 cut(s) 51, 195, 319, 672
Hpy188III TCNNGA 4 cut(s) 23, 61, 205, 467
Hpy8I GTNNAC 3 cut(s) 133, 230, 587
HpyAV CCTTC 2 cut(s) 77, 433
HpyCH4III ACNGT 4 cut(s) 80, 172, 771, 830
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 4 cut(s) 90, 593, 782, 835
HpyF10VI GCNNNNNNNGC 3 cut(s) 26, 716, 788
HpyF3I CTNAG 4 cut(s) 316, 402, 483, 644
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 6 cut(s) 57, 236, 351, 479, 493, 667
Ksp22I TGATCA 2 cut(s) 619, 802
Kzo9I GATC 6 cut(s) 57, 602, 619, 640, 676, 802
LmnI GCTCC 2 cut(s) 584, 745
Lsp1109I GCAGC 2 cut(s) 719, 794
LweI GCATC 1 cut(s) 769
MaeI CTAG 1 cut(s) 786
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 7 cut(s) 166, 172, 350, 392, 443, 695, 712
MalI GATC 6 cut(s) 59, 604, 621, 642, 678, 804
MboI GATC 6 cut(s) 57, 602, 619, 640, 676, 802
MboII GAAGA 1 cut(s) 115
MflI RGATCY 3 cut(s) 57, 602, 676
MlsI TGGCCA 2 cut(s) 247, 474
MluCI AATT 2 cut(s) 120, 321
MluNI TGGCCA 2 cut(s) 247, 474
MlyI GAGTC 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 7 cut(s) 40, 87, 142, 209, 325, 382, 409
Mox20I TGGCCA 2 cut(s) 247, 474
MscI TGGCCA 2 cut(s) 247, 474
MseI TTAA 2 cut(s) 408, 825
MslI CAYNNNNRTG 1 cut(s) 615
Msp20I TGGCCA 2 cut(s) 247, 474
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 2 cut(s) 369, 455
MspR9I CCNGG 1 cut(s) 370
MwoI GCNNNNNNNGC 3 cut(s) 26, 716, 788
NciI CCSGG 1 cut(s) 370
NcoI CCATGG 1 cut(s) 489
NdeII GATC 6 cut(s) 57, 602, 619, 640, 676, 802
NlaIII CATG 6 cut(s) 57, 236, 351, 479, 493, 667
NlaIV GGNNCC 1 cut(s) 741
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 6 cut(s) 166, 172, 350, 392, 443, 695
NspI RCATGY 3 cut(s) 236, 351, 667
PciI ACATGT 2 cut(s) 347, 663
PfeI GAWTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 709, 784
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PscI ACATGT 2 cut(s) 347, 663
PspN4I GGNNCC 1 cut(s) 741
PspPI GGNCC 3 cut(s) 587, 632, 758
PstNI CAGNNNCTG 2 cut(s) 176, 710
PsuI RGATCY 3 cut(s) 57, 602, 676
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 2 cut(s) 343, 773
RsaNI GTAC 2 cut(s) 342, 772
RseI CAYNNNNRTG 1 cut(s) 615
SaqAI TTAA 2 cut(s) 408, 825
SatI GCNGC 2 cut(s) 708, 783
Sau3AI GATC 6 cut(s) 57, 602, 619, 640, 676, 802
Sau96I GGNCC 3 cut(s) 587, 632, 758
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 370
SfaNI GCATC 1 cut(s) 769
SinI GGWCC 3 cut(s) 587, 632, 758
SmiMI CAYNNNNRTG 1 cut(s) 615
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 2 cut(s) 120, 321
SsiI CCGC 1 cut(s) 452
SspMI CTAG 1 cut(s) 786
StyD4I CCNGG 1 cut(s) 368
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 4 cut(s) 80, 172, 771, 830
TaiI ACGT 1 cut(s) 192
TasI AATT 2 cut(s) 120, 321
TatI WGTACW 2 cut(s) 341, 771
TfiI GAWTC 1 cut(s) 479
Tru1I TTAA 2 cut(s) 408, 825
Tru9I TTAA 2 cut(s) 408, 825
TscAI CASTG 6 cut(s) 83, 181, 631, 726, 835, 850
TseFI GTSAC 6 cut(s) 166, 172, 350, 392, 443, 695
TseI GCWGC 2 cut(s) 707, 782
Tsp45I GTSAC 6 cut(s) 166, 172, 350, 392, 443, 695
TspDTI ATGAA 1 cut(s) 864
TspRI CASTG 6 cut(s) 83, 181, 631, 726, 835, 850
VpaK11BI GGWCC 3 cut(s) 587, 632, 758
XapI RAATTY 1 cut(s) 321
XceI RCATGY 3 cut(s) 236, 351, 667
XspI CTAG 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.