Rw1G026430

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000194
Physical Location & Seq
Forward (+)
53560148 .. 53562679
2532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G026430.1

Sequence Viewer

Length: 2277 bp
ATGGCAAGCCTTGTTACTGAGAATCAGTCGGCCTTTGTCTCAGATAGACAAATTCAAGACAATATTGTTGTTGCTCATGAGCTTTTTCATCGGTTAAAGCTCCGCCGATCAGGGAACAATGCAGCTTTTGGTTTAAAGCTAGATATGAATAAGGCCTATGATCGTATTGAGTGGGATTTCTTGCAGGCAGTCCTAAAAAAGATGGGTTTTGCAGATCAATGGGTGACTTTGGTTATGAATTGTGTCCGAACTTCCTCCCTTTCTGTGCTTCTTAATGGGAAACCAGGGCCTTTTTTCAAACCAACCCGTGGTCTCAGACAAGGAGATCCCTTGTCACCTTTTCTTTTTCTTTTTGTTAATGACGTTCTTTCAAAAATGGTGATTAAATGTTGCAGATGCTCCCTACTGAAACCTGTGACTATTGGTCCACAAGAATTAGGGATCAGCCATCTTTTCTTTGCAGATGACTCCCTGTTCTTCCTTCAAGCCACTCTACAGAACTGTGAAATGTTGTCTGATCTCCTCCATACTTATTGTGTAGCTTCGGGGCAACTGATAAATGTGGACAAATCTTCTATTTTTTTCAGTCCAAATACACAACCTGAAATTGTTCTTTTGTTGAGTGCTGTTATGCAAATCCCAGTTGTTTTTGATCCAGGAAAATACTTGGGGCTTCCAACCTTCTGGCATCGATCAAAAAAGGCTGCTCTTGGTTTTATCAAGGACTCAATATCCAAGAAAGTTCAGGGATGGAAACAGGCTACTCTTAGTCAAGCTGGGAAAGAAACTCTTATCAAGGCTATTGCCACGGCTATCCCAGCGTATCCTATGGCATGTTTTAAATTTCCAGTCTGTCTTTGCACTCAATTGAATGGTATTTTGGCAAATTTCTGGTGGGGAAATCAAGATTCTAATGGACTACATTGGAAGTCTTGGAATTTCCTAAGTTTGCCTAAACAAGACGGTGGAATGGGATTCAGAAATTTGGTAGAATTTAATGATTCTTTGCTGGCTAAACAAGCTTGGAGACTTACTCACAATCCCACTTCTTTATGGGCTCGTGTCCTGCAGCAACTGTATTTCCCATCGACTTCTTTTCGTTTCGCAAGAAAAGGTGCTTCAGCTTCTTGGATTTGGTCAAGCCTGTTAATTGGTAGAGATGTTATTCATTCTGAATCTCTTTGGAACATTGGTAATGGTACTTCTGTTCAGATAGGCAGTGATACATGGATCCCTTCCAACCCTCCCTCATTGATCCATCTTCCGCTGGACTATGGGAACTTTCAGGTTGGTTCTCTCATCAATTGGAACACAAAAACGTGGAATCTGGAGCAAGTCAAAGACCTCATTACACCTGCACAATCCCTCTTGATTTCTGCTATTCCTATTACTGATAAAAACATTTCTGATACGTTGATATGGCCTCACACCAGAAACGGTGGTTTTACTGTTAAGTCAGGTTATCATCTCCTTCACTCTCGTTCTCAAGTTGGAGTAATGCAACATCCTCATAATTCATATGCTATTCCTAGCAGTCTTTGGTCCTGGATTTGGAAGCTTCAAACTATTCCTCGAGTCAAAAATTTCTTATGGCGTGCTTGTCATGGTATTCTTCCAACAAAGAGCAATTTATTTAGAAGGCATATTGCATCTTCAGCTGAGTGTCAATTATGCCTGCACCCAAATGAAACAACTGAACATGCTCTTCTCACTTGTCCTTGGATTGTAAGTGCTTGGTTTGCTCATCCCCTATCTTACAAAGTTCCTCTACAAGCTATCACTTCTTTTGAAAGTTGGCTCTTCAACATAAGCCAAATGGATACCCCTGCTGATTTTGATACCCTTATGTGTGGAGTGGCAGCTTTGGCTAGAAACTGGAAAGGTTCTTTGATTGCAGGTAAAACGGTTAAAATGCGCGCTCCTTCCCCTTTGGCAGCAGAAGCATTAGCAGTTCGGGAAGCAATTCTTCTGGCAAACTCCTTCCCTCATATGGATATCATTATCTCTTCAGATTCTCAAACTTTGATCAACTCCATCTCCCATAATCTCCCTGCCTCTGATTGGAAAGCTGCAACCATTATTTCTCAAGTCCGGTACCTCTCTTCAACCCGGCAGTTTAACTGGTCCTGGATCAGTAGAAAGGCTAATAAAGCAGCTCATCATGTTGCTTCATTAGTTAACAGTGGGAAGTGTCCTTCCAACTGGGTTCGTCACCCTCCCTCCTCTTTATTTGAAATTTTGTTGTTTGATGGGCTTCCAGGTCCACCTCTAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

758

Amino Acids

84.85

Weight (kDa)

9.41

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 4 - 225 1.3e-18 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 482 - 579 1.5e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 616 - 724 7.8e-12 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1363
Acc36I ACCTGC 2 cut(s) 1363, 1886
Acc65I GGTACC 1 cut(s) 2094
AccB1I GGYRCC 1 cut(s) 2094
AccB7I CCANNNNNTGG 1 cut(s) 308
AccII CGCG 1 cut(s) 1917
AciI CCGC 2 cut(s) 103, 1265
AclWI GGATC 7 cut(s) 320, 449, 647, 1225, 1238, 1249, 2138
AcsI RAATTY 8 cut(s) 51, 842, 886, 937, 982, 992, 1582, 2235
AcuI CTGAAG 3 cut(s) 1104, 1638, 1992
AfaI GTAC 2 cut(s) 1201, 2096
AfiI CCNNNNNNNGG 4 cut(s) 308, 1551, 1990, 2061
AhdI GACNNNNNGTC 1 cut(s) 423
AjnI CCWGG 5 cut(s) 283, 655, 1544, 2126, 2257
AjuI GAANNNNNNNTTGG 2 cut(s) 863, 895
Alw26I GTCTC 3 cut(s) 43, 317, 1021
AlwI GGATC 7 cut(s) 320, 449, 647, 1225, 1238, 1249, 2138
AlwNI CAGNNNCTG 1 cut(s) 1075
Ama87I CYCGRG 1 cut(s) 1572
AoxI GGCC 4 cut(s) 30, 153, 287, 1421
ApeKI GCWGC 7 cut(s) 122, 704, 1069, 1859, 1934, 2069, 2153
ApoI RAATTY 8 cut(s) 51, 842, 886, 937, 982, 992, 1582, 2235
Asp700I GAANNNNTTC 2 cut(s) 609, 1962
Asp718I GGTACC 1 cut(s) 2094
AspLEI GCGC 2 cut(s) 1917, 1919
AspS9I GGNCC 5 cut(s) 287, 425, 1542, 2124, 2261
AsuC2I CCSGG 1 cut(s) 2110
AsuHPI GGTGA 4 cut(s) 235, 327, 391, 2204
AvaI CYCGRG 1 cut(s) 1572
AvaII GGWCC 4 cut(s) 425, 1542, 2124, 2261
BaeI ACNNNNGTAYC 2 cut(s) 1191, 1224
BamHI GGATCC 1 cut(s) 1230
BanI GGYRCC 1 cut(s) 2094
BanII GRGCYC 1 cut(s) 1060
BarI GAAGNNNNNNTAC 2 cut(s) 1186, 1218
BauI CACGAG 1 cut(s) 1059
BbvI GCAGC 7 cut(s) 134, 691, 1081, 1871, 1946, 2056, 2165
BccI CCATC 7 cut(s) 196, 456, 744, 1093, 1266, 2042, 2243
BceAI ACGGC 1 cut(s) 825
BciT130I CCWGG 5 cut(s) 285, 657, 1546, 2128, 2259
BciVI GTATCC 2 cut(s) 834, 1813
BclI TGATCA 1 cut(s) 2025
BcnI CCSGG 1 cut(s) 2110
BcoDI GTCTC 3 cut(s) 43, 317, 1021
BfaI CTAG 4 cut(s) 140, 1530, 1869, 2270
BfmI CTRYAG 2 cut(s) 494, 1067
BfuAI ACCTGC 2 cut(s) 1363, 1886
BfuI GTATCC 2 cut(s) 834, 1813
BisI GCNGC 7 cut(s) 123, 705, 1070, 1860, 1935, 2070, 2154
BlsI GCNGC 7 cut(s) 124, 706, 1071, 1861, 1936, 2071, 2155
Bme1390I CCNGG 6 cut(s) 285, 657, 1546, 2110, 2128, 2259
Bme18I GGWCC 4 cut(s) 425, 1542, 2124, 2261
BmeRI GACNNNNNGTC 1 cut(s) 423
BmeT110I CYCGRG 1 cut(s) 1572
BmgT120I GGNCC 5 cut(s) 287, 425, 1542, 2124, 2261
BmiI GGNNCC 2 cut(s) 1232, 2096
BmrFI CCNGG 6 cut(s) 285, 657, 1546, 2110, 2128, 2259
BmrI ACTGGG 2 cut(s) 635, 2212
BmsI GCATC 3 cut(s) 386, 697, 1658
BmuI ACTGGG 2 cut(s) 635, 2212
BplI GAGNNNNNCTC 2 cut(s) 1018, 1050
BpmI CTGGAG 1 cut(s) 1349
BpuEI CTTGAG 2 cut(s) 1470, 2070
BpuMI CCSGG 1 cut(s) 2110
Bsa29I ATCGAT 1 cut(s) 691
BsaI GGTCTC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 284, 307, 807, 1718
BsaWI WCCGGW 1 cut(s) 2091
BsaXI ACNNNNNCTCC 4 cut(s) 2021, 2051, 2204, 2234
Bsc4I CCNNNNNNNGG 4 cut(s) 308, 1551, 1990, 2061
Bse1I ACTGG 5 cut(s) 641, 848, 1880, 2126, 2207
BseBI CCWGG 5 cut(s) 285, 657, 1546, 2128, 2259
BseCI ATCGAT 1 cut(s) 691
BseDI CCNNGG 4 cut(s) 284, 307, 807, 1718
BseGI GGATG 3 cut(s) 755, 1504, 1744
BseLI CCNNNNNNNGG 4 cut(s) 308, 1551, 1990, 2061
BseMII CTCAG 4 cut(s) 9, 54, 328, 1650
BseNI ACTGG 5 cut(s) 641, 848, 1880, 2126, 2207
BsePI GCGCGC 1 cut(s) 1915
BseRI GAGGAG 2 cut(s) 512, 2212
BseXI GCAGC 7 cut(s) 134, 691, 1081, 1871, 1946, 2056, 2165
BseYI CCCAGC 2 cut(s) 776, 817
BsgI GTGCAG 2 cut(s) 1341, 1661
Bsh1236I CGCG 1 cut(s) 1917
BshFI GGCC 4 cut(s) 32, 155, 289, 1423
BshNI GGYRCC 1 cut(s) 2094
BshVI ATCGAT 1 cut(s) 691
BsiHKCI CYCGRG 1 cut(s) 1572
BsiSI CCGG 2 cut(s) 2092, 2110
BslI CCNNNNNNNGG 4 cut(s) 308, 1551, 1990, 2061
BsmAI GTCTC 3 cut(s) 43, 317, 1021
BsnI GGCC 4 cut(s) 32, 155, 289, 1423
Bso31I GGTCTC 1 cut(s) 317
BsoBI CYCGRG 1 cut(s) 1572
Bsp1286I GDGCHC 1 cut(s) 1060
BspACI CCGC 2 cut(s) 103, 1265
BspANI GGCC 4 cut(s) 32, 155, 289, 1423
BspCNI CTCAG 4 cut(s) 10, 53, 327, 1651
BspDI ATCGAT 1 cut(s) 691
BspFNI CGCG 1 cut(s) 1917
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 2 cut(s) 1232, 2096
BspMAI CTGCAG 1 cut(s) 1071
BspMI ACCTGC 2 cut(s) 1363, 1886
BspPI GGATC 7 cut(s) 320, 449, 647, 1225, 1238, 1249, 2138
BspQI GCTCTTC 2 cut(s) 1710, 1805
BspT107I GGYRCC 1 cut(s) 2094
BspTNI GGTCTC 1 cut(s) 317
BsrI ACTGG 5 cut(s) 641, 848, 1880, 2126, 2207
BssECI CCNNGG 4 cut(s) 284, 307, 807, 1718
BssHII GCGCGC 1 cut(s) 1915
BssSI CACGAG 1 cut(s) 1059
BssT1I CCWWGG 1 cut(s) 1718
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 5 cut(s) 285, 657, 1546, 2128, 2259
Bst4CI ACNGT 7 cut(s) 503, 965, 1077, 1439, 1450, 1906, 2183
Bst6I CTCTTC 4 cut(s) 1710, 1805, 2011, 2107
BstC8I GCNNGC 6 cut(s) 7, 186, 1011, 1596, 1676, 1917
BstDEI CTNAG 6 cut(s) 18, 40, 314, 767, 944, 1659
BstDSI CCRYGG 2 cut(s) 307, 807
BstF5I GGATG 3 cut(s) 755, 1504, 1744
BstFNI CGCG 1 cut(s) 1917
BstHHI GCGC 2 cut(s) 1917, 1919
BstMAI GTCTC 3 cut(s) 43, 317, 1021
BstMWI GCNNNNNNNGC 7 cut(s) 818, 1019, 1655, 1739, 1865, 1940, 2150
BstNI CCWGG 5 cut(s) 285, 657, 1546, 2128, 2259
BstNSI RCATGY 2 cut(s) 837, 1703
BstSCI CCNGG 6 cut(s) 283, 655, 1544, 2108, 2126, 2257
BstSFI CTRYAG 2 cut(s) 494, 1067
BstUI CGCG 1 cut(s) 1917
BstV1I GCAGC 7 cut(s) 134, 691, 1081, 1871, 1946, 2056, 2165
BstX2I RGATCY 2 cut(s) 325, 1230
BstXI CCANNNNNNTGG 1 cut(s) 684
BstYI RGATCY 2 cut(s) 325, 1230
Bsu15I ATCGAT 1 cut(s) 691
BsuI GTATCC 2 cut(s) 834, 1813
BsuRI GGCC 4 cut(s) 32, 155, 289, 1423
BsuTUI ATCGAT 1 cut(s) 691
BtgI CCRYGG 2 cut(s) 307, 807
BtsCI GGATG 3 cut(s) 755, 1504, 1744
BtsI GCAGTG 1 cut(s) 1225
BtsIMutI CAGTG 2 cut(s) 1225, 2188
BveI ACCTGC 2 cut(s) 1363, 1886
Cac8I GCNNGC 6 cut(s) 7, 186, 1011, 1596, 1676, 1917
CaiI CAGNNNCTG 1 cut(s) 1075
CciI TCATGA 1 cut(s) 76
CfoI GCGC 2 cut(s) 1917, 1919
Cfr13I GGNCC 5 cut(s) 287, 425, 1542, 2124, 2261
ClaI ATCGAT 1 cut(s) 691
Csp6I GTAC 2 cut(s) 1200, 2095
CviAII CATG 6 cut(s) 77, 834, 1227, 1604, 1700, 2162
CviQI GTAC 2 cut(s) 1200, 2095
DdeI CTNAG 6 cut(s) 18, 40, 314, 767, 944, 1659
DraI TTTAAA 2 cut(s) 135, 841
DriI GACNNNNNGTC 1 cut(s) 423
Eam1104I CTCTTC 4 cut(s) 1710, 1805, 2011, 2107
Eam1105I GACNNNNNGTC 1 cut(s) 423
EarI CTCTTC 4 cut(s) 1710, 1805, 2011, 2107
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 1718
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 1 cut(s) 1060
Eco31I GGTCTC 1 cut(s) 317
Eco32I GATATC 1 cut(s) 1996
Eco47I GGWCC 4 cut(s) 425, 1542, 2124, 2261
Eco57I CTGAAG 3 cut(s) 1104, 1638, 1992
Eco88I CYCGRG 1 cut(s) 1572
EcoO109I RGGNCCY 1 cut(s) 287
EcoRII CCWGG 5 cut(s) 283, 655, 1544, 2126, 2257
EcoRV GATATC 1 cut(s) 1996
EcoT14I CCWWGG 1 cut(s) 1718
EcoT38I GRGCYC 1 cut(s) 1060
ErhI CCWWGG 1 cut(s) 1718
FaeI CATG 6 cut(s) 80, 837, 1230, 1607, 1703, 2165
FalI AAGNNNNNCTT 4 cut(s) 774, 806, 1950, 1982
FatI CATG 6 cut(s) 76, 833, 1226, 1603, 1699, 2161
FauNDI CATATG 2 cut(s) 1519, 1989
FbaI TGATCA 1 cut(s) 2025
Fnu4HI GCNGC 7 cut(s) 123, 705, 1070, 1860, 1935, 2070, 2154
FokI GGATG 3 cut(s) 762, 1491, 1731
FriOI GRGCYC 1 cut(s) 1060
Fsp4HI GCNGC 7 cut(s) 123, 705, 1070, 1860, 1935, 2070, 2154
FspBI CTAG 4 cut(s) 140, 1530, 1869, 2270
GlaI GCGC 2 cut(s) 1916, 1918
GluI GCNGC 7 cut(s) 123, 705, 1070, 1860, 1935, 2070, 2154
GsaI CCCAGC 2 cut(s) 780, 821
GsuI CTGGAG 1 cut(s) 1349
HaeIII GGCC 4 cut(s) 32, 155, 289, 1423
HapII CCGG 2 cut(s) 2092, 2110
HhaI GCGC 2 cut(s) 1917, 1919
Hin1II CATG 6 cut(s) 80, 837, 1230, 1607, 1703, 2165
Hin6I GCGC 2 cut(s) 1915, 1917
HinP1I GCGC 2 cut(s) 1915, 1917
HincII GTYRAC 1 cut(s) 2179
HindII GTYRAC 1 cut(s) 2179
HindIII AAGCTT 2 cut(s) 1020, 1556
HpaI GTTAAC 1 cut(s) 2179
HpaII CCGG 2 cut(s) 2092, 2110
HphI GGTGA 4 cut(s) 235, 327, 391, 2204
Hpy166II GTNNAC 4 cut(s) 428, 565, 2179, 2264
Hpy188III TCNNGA 6 cut(s) 56, 77, 905, 1328, 1369, 1955
Hpy8I GTNNAC 4 cut(s) 428, 565, 2179, 2264
HpyAV CCTTC 8 cut(s) 491, 691, 1245, 1481, 1632, 1932, 1990, 2205
HpyCH4III ACNGT 7 cut(s) 503, 965, 1077, 1439, 1450, 1906, 2183
HpyCH4IV ACGT 3 cut(s) 363, 1319, 1412
HpyF10VI GCNNNNNNNGC 7 cut(s) 818, 1019, 1655, 1739, 1865, 1940, 2150
HpyF3I CTNAG 6 cut(s) 18, 40, 314, 767, 944, 1659
HpySE526I ACGT 3 cut(s) 363, 1319, 1412
Hsp92II CATG 6 cut(s) 80, 837, 1230, 1607, 1703, 2165
HspAI GCGC 2 cut(s) 1915, 1917
KpnI GGTACC 1 cut(s) 2098
Ksp22I TGATCA 1 cut(s) 2025
KspAI GTTAAC 1 cut(s) 2179
LguI GCTCTTC 2 cut(s) 1710, 1805
LmnI GCTCC 4 cut(s) 105, 404, 1330, 1924
Lsp1109I GCAGC 7 cut(s) 134, 691, 1081, 1871, 1946, 2056, 2165
LweI GCATC 3 cut(s) 386, 697, 1658
MaeI CTAG 4 cut(s) 140, 1530, 1869, 2270
MaeII ACGT 3 cut(s) 363, 1319, 1412
MaeIII GTNAC 5 cut(s) 13, 223, 333, 415, 2210
MfeI CAATTG 2 cut(s) 866, 1303
MflI RGATCY 2 cut(s) 325, 1230
MhlI GDGCHC 1 cut(s) 1060
MlyI GAGTC 3 cut(s) 461, 719, 1584
MmeI TCCRAC 5 cut(s) 701, 1263, 1471, 1640, 2223
MroXI GAANNNNTTC 2 cut(s) 609, 1962
MslI CAYNNNNRTG 1 cut(s) 1683
MspA1I CMGCKG 2 cut(s) 1267, 1658
MspI CCGG 2 cut(s) 2092, 2110
MspR9I CCNGG 6 cut(s) 285, 657, 1546, 2110, 2128, 2259
MunI CAATTG 2 cut(s) 866, 1303
MvaI CCWGG 5 cut(s) 285, 657, 1546, 2128, 2259
MvnI CGCG 1 cut(s) 1917
MwoI GCNNNNNNNGC 7 cut(s) 818, 1019, 1655, 1739, 1865, 1940, 2150
NciI CCSGG 1 cut(s) 2110
NdeI CATATG 2 cut(s) 1519, 1989
NlaIII CATG 6 cut(s) 80, 837, 1230, 1607, 1703, 2165
NlaIV GGNNCC 2 cut(s) 1232, 2096
NmuCI GTSAC 4 cut(s) 223, 333, 415, 2210
NspI RCATGY 2 cut(s) 837, 1703
PaeR7I CTCGAG 1 cut(s) 1572
PagI TCATGA 1 cut(s) 76
PaqCI CACCTGC 1 cut(s) 1363
PauI GCGCGC 1 cut(s) 1915
PceI AGGCCT 1 cut(s) 155
PciSI GCTCTTC 2 cut(s) 1710, 1805
PdmI GAANNNNTTC 2 cut(s) 609, 1962
PfeI GAWTC 7 cut(s) 22, 908, 975, 1001, 1175, 1324, 2012
PflMI CCANNNNNTGG 1 cut(s) 308
PfoI TCCNGGA 3 cut(s) 655, 1544, 2126
PkrI GCNGC 7 cut(s) 124, 706, 1071, 1861, 1936, 2071, 2155
PleI GAGTC 3 cut(s) 461, 719, 1583
PpsI GAGTC 3 cut(s) 461, 719, 1583
Psp6I CCWGG 5 cut(s) 283, 655, 1544, 2126, 2257
PspFI CCCAGC 2 cut(s) 776, 817
PspGI CCWGG 5 cut(s) 283, 655, 1544, 2126, 2257
PspN4I GGNNCC 2 cut(s) 1232, 2096
PspPI GGNCC 5 cut(s) 287, 425, 1542, 2124, 2261
PspXI VCTCGAGB 1 cut(s) 1572
PstI CTGCAG 1 cut(s) 1071
PstNI CAGNNNCTG 1 cut(s) 1075
PsuI RGATCY 2 cut(s) 325, 1230
PteI GCGCGC 1 cut(s) 1915
PvuII CAGCTG 1 cut(s) 1658
RsaI GTAC 2 cut(s) 1201, 2096
RsaNI GTAC 2 cut(s) 1200, 2095
RseI CAYNNNNRTG 1 cut(s) 1683
SapI GCTCTTC 2 cut(s) 1710, 1805
SatI GCNGC 7 cut(s) 123, 705, 1070, 1860, 1935, 2070, 2154
Sau96I GGNCC 5 cut(s) 287, 425, 1542, 2124, 2261
SchI GAGTC 3 cut(s) 461, 719, 1584
ScrFI CCNGG 6 cut(s) 285, 657, 1546, 2110, 2128, 2259
SduI GDGCHC 1 cut(s) 1060
SfaNI GCATC 3 cut(s) 386, 697, 1658
SfcI CTRYAG 2 cut(s) 494, 1067
Sfr274I CTCGAG 1 cut(s) 1572
SinI GGWCC 4 cut(s) 425, 1542, 2124, 2261
SlaI CTCGAG 1 cut(s) 1572
SmiMI CAYNNNNRTG 1 cut(s) 1683
SmlI CTYRAG 3 cut(s) 1485, 1572, 2085
SmoI CTYRAG 3 cut(s) 1485, 1572, 2085
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 2 cut(s) 103, 1265
SspI AATATT 1 cut(s) 64
SspMI CTAG 4 cut(s) 140, 1530, 1869, 2270
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 6 cut(s) 283, 655, 1544, 2108, 2126, 2257
StyI CCWWGG 1 cut(s) 1718
TaaI ACNGT 7 cut(s) 503, 965, 1077, 1439, 1450, 1906, 2183
TaiI ACGT 3 cut(s) 366, 1322, 1415
TaqI TCGA 3 cut(s) 691, 1088, 1573
TfiI GAWTC 7 cut(s) 22, 908, 975, 1001, 1175, 1324, 2012
TscAI CASTG 2 cut(s) 1225, 2188
TseFI GTSAC 4 cut(s) 223, 333, 415, 2210
TseI GCWGC 7 cut(s) 122, 704, 1069, 1859, 1934, 2069, 2153
Tsp45I GTSAC 4 cut(s) 223, 333, 415, 2210
TspDTI ATGAA 7 cut(s) 77, 161, 251, 1157, 1506, 1701, 2160
TspRI CASTG 2 cut(s) 1225, 2188
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 4 cut(s) 425, 1542, 2124, 2261
XapI RAATTY 8 cut(s) 51, 842, 886, 937, 982, 992, 1582, 2235
XceI RCATGY 2 cut(s) 837, 1703
XhoI CTCGAG 1 cut(s) 1572
XmnI GAANNNNTTC 2 cut(s) 609, 1962
XspI CTAG 4 cut(s) 140, 1530, 1869, 2270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.