Prupe.6G087800_v2.0.a1

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000194
Physical Location & Seq
Reverse (-)
5953215 .. 5956897
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G087800.1

Sequence Viewer

Length: 3408 bp
ATGGCCCCCCCATATCGGGAGGAAAAAGCATCCTTTTGGGAAAACTTGGCAAACTTTGCTATGGTCGACTCCCCCCCGTGGCTCTGTATCGGAGATATGAATGAGATATTAGGAAACGACGAAAAAGAAGGCGGCATACCTTGGCCTTGGGGCCGTACTCGATATCTTCGATCCTTTATGGACACTAATGGATTGCTGGAATTAAGATCGACGAGCCAATTCTACACTTGGGAGAATCAACATGACCCAGTCTCACTCATTCGAATGAAACTGGACCGTGGGCTTATCAATGACCAATGGCTCTTTTTGTGGCCGGACTTGTGTGTGCATGTGGAGCCCAGGGTTGGCTCTGACCACTCCCCTCTGGTGCTTTACTTTGCTCCAAAAATTCAGAGGCGAGCAGGAGGTTTTAAGTTTGAAGCTTACTGGGCTGATGAACACGATTGTGGAACCATCATCCAGAGGGGCTGGAAGAATGACATTGTTGGGGATTCTTTTGCTGCTCTGTCGGCTAATCTTGGTGTTTGTAGAGAGGAGCTCCAGAAATGGAGCAAAGAAAAATTTCCAAACAACCTGTCTCGCATAAACCTCTTGATGAAAAGTCTGAGTAATCTCCAGTCGGGGCCACTTGAGGAAAATTATCGACACCAGGAATCAGCCATTTGGGACGAAATGTCCGTCTTGTGGAGCAGGGAGGAAACTTACTGGAAACAGCGCTCGAGGTTGAACTGGTTGTCGGCGGGTGACGCAAACACGAAGTTTTTCCATACCACAACCCTACAACGCAGACAACGAAACAAAATAGAAACCTTATTGAAATCGGAAGGAAATTGCATAAGTGGAGACCAAGCGATCAGGGAGGAGTTTGGCATTTTCTTTGGTAACCTGTTCAAGTCTGGGGGTCCTAGAAACTGGGGAGGTATTCTAAATTGCGTTCATGCTAGCATCACCGAAGCCCAAAACAAAAGGTTAACCGATCCCTTCTCGATGGAGGAAGTCCGAACTGCAGTCAAACAGCTCGGTTCCTTGAAAGCGCCTGGTCCGGATGGCTTCCCTGGCCTCTTCTATGATCGCTTCTGGGAAATTATTCAAAATTTTGTTAATAACTTGGTTACAGATTTTTTCTTAGGCAATAGCAGATTGGAGCTCCTAAATCACACTCATATTGTCCTCATCCCAAAGATTCCCAAGCCAACGTCGGTAAACCATTTTCGACCTATTAGTTTATGCAACAACTCGTATAAGATTCTGTCTAAACTACTGGCAAATCGTCTCAAGACCCTTCTCCCTATGCTGATAAGTCAGCATCAGAATGCCTTTGTCCCGGGACGGCAGATACAAGACAATATTTTATTGGCCCACGAGGCTTTCCATTATCTCCGTCTTAAGTCTTCCAAGAAATCCTTTGAGCTGGGACTGAAACTGGACATGAATAAGGCATACGACCGGGTCGAATGGGATTTCCTTGAGGCCACTCTTTGTAAGTTCAGCTTCGACAACCGTTGGGTGGAGCTGGTCATGCTATGTGTCAAAACTATCACTTTCTCTCTGGTATTGAATGGTTCCCCTGGCAGTCCCTTTTCCCCATCGAGGGGCCTGAGACAAGGAGATCCCTTATCTCCCTATCTTTTTCTTCTTGTCTCCGAGGTTCTTTCTCTAAATATTATCAATTCTACTGATACTGGGATGCTTAGGGGCATTAAATTAAGCAGGGGTGGTCCGGAATTATCCCACCTATTCTTTGCAGACGACTCTCTGTTTTTTCTACAAGCTACTCCTCCGAACTGCTCGGCTCTTAAGTCCATTATCGAATGCTACTGCTCGGCGTCGGGTCAGGAGGTGAATTTTAACAAATCCAGTCTGTACTTCTCTCCCAACACTGATACTGGGACTAGGAATCAGTTCGGCCTCATCCTTGGCGGCCGCTCTAAGGTGGAGGCCTTGGCCTATGTTCGGGACAGAATAAATTCGAAGATTGCTGGGTGGAAGCTAAAGCTATTATCTCAAGCGGTCAGGGAGGTCCTTATCAAATCGGTGGCTGCAGCTATCCCGGCCCACCCCATGTCTTGCTTCTTGCTGCCAGCCACTATATGCAATTCAATAAACACTGATTTGGCGAGATTCTGCGGAGGGGGCATGGGATTTCTCGATTTACAGGCTTTCAACCGGTCCCTACTTGCCAAGCAATGTTGGCGAATCATGCGCAACCCGAATGCTCTTTGGGCTAGGATTTTAAAGGCCCGCTACTTCCCCGAGTGTTCCTTCCTCGATGCTAAGAAGGGAGGGCGTGCGTCATGGGCTTTGAGCAGCCTCCTAGTCGGTCGAGATATCATCGAGAAGGGAGCTTACTGGCAAATTGGAAACGGCCGTTCAGTGAGTGTGTGGAAGGACCGCTGGTTAATGGGGTATGGCTCCGGAAAAATCTCCCCCCTCCCCTCCAGTAACCGCTTCACCCCCCTGCTGGTGGCCGATCTCATCGATGTGGGGAACAGGTCCTGGAACATCTCACACATTGAGCCCTTTATTCCTCCTAGCGAGGCTATGTTAATCCGATCTACTCCTATCGGCTCCCTGGCTACTCGTGACCCTCTAGTGTGGCCGGCGGTAAAGAATGGTGACTATACTGTTAAATCCGGGTACTACCATGCCATCAACGTCTCGCCTCCTGACCCCTGTGATCGAGCTTCTTCCTCCCATGCAGTGAATTCCGACGTGTGGAAAGTTATCTGGAGGGCTCACATCACTCCCAAAATCAGGAACTTTATGTGGCGTGCGTTAACAAACTCCATTCCTACGTGTGCTAACCTCTTTGGAAGAAAGTTAGCGCGTTCTCCTACGTGCCGCTTGTGTGGTTTGTTTCCTGAGACTGTGGAGCACCTGTTACTGCTTTGTTCTTGGACGCGGGCAGTATGGTTTGGCTGCCCTTTTGGTTACGCTCCGAACCTGGCATCCATTACCACACTGGATTCTTGGCTTTCTAGCTTCCTAAGAAGCTCTTTTGCCGATGGAGATCAAAGGGACTGGGGAATTTCCCTCTTCATGTTCTGCTCTTGGGAAATTTGGAAGGCTCGATGTAAGGCGATTTTCAATGATATCCGGCCCTCTCCTCCCCTGGCTAAAACGGGTGTGGGGGGTATTGGAGCGGTTATTCGCGATCATAACGGCTCCTTCATCGGTGCCGCTAGTCAACCATGCAATTGTTCGTCTGCGGCTGAATGTGAAGCTAGTGCAGCGATCATGGGCCTCTCTTTTGCCTCCTCCCTTCACGTGCAGAATGTGGTTGTGGAGACTGACTACTCTGAGCTAGTTTCTTGTGTAAAGAAGGGTTCAGCATCTGGTAACTGGAGATTCTACCTTTTCCTCGCGGAATTCCGCAGCCTGGAAGCGTCCTTCATCCAATGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

1136

Amino Acids

127.77

Weight (kDa)

8.93

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2204
AccB1I GGYRCC 1 cut(s) 3176
AccBSI CCGCTC 2 cut(s) 1926, 3143
AccI GTMKAC 1 cut(s) 66
AccII CGCG 4 cut(s) 2827, 2902, 3153, 3365
AccIII TCCGGA 3 cut(s) 1042, 1720, 2414
AclWI GGATC 3 cut(s) 165, 971, 1604
AcoI YGGCCR 5 cut(s) 311, 1921, 2365, 2466, 2597
AcsI RAATTY 9 cut(s) 387, 560, 1093, 1843, 1966, 2704, 3027, 3057, 3368
AcvI CACGTG 1 cut(s) 3268
AcyI GRCGYC 1 cut(s) 1826
AfaI GTAC 3 cut(s) 157, 1865, 2639
AfeI AGCGCT 1 cut(s) 716
AflII CTTAAG 2 cut(s) 1385, 1796
AflIII ACRYGT 2 cut(s) 2712, 2795
AgeI ACCGGT 1 cut(s) 2166
AhdI GACNNNNNGTC 1 cut(s) 673
AjiI CACGTC 1 cut(s) 2713
AleI CACNNNNGTG 1 cut(s) 444
AloI GAACNNNNNNTCC 2 cut(s) 2933, 2965
Alw21I GWGCWC 3 cut(s) 540, 1149, 2877
Alw26I GTCTC 9 cut(s) 256, 582, 837, 1277, 1594, 1645, 2662, 2858, 3281
AlwI GGATC 3 cut(s) 165, 971, 1604
AlwNI CAGNNNCTG 2 cut(s) 2496, 3335
Ama87I CYCGRG 3 cut(s) 718, 1324, 2252
Aor13HI TCCGGA 3 cut(s) 1042, 1720, 2414
Aor51HI AGCGCT 1 cut(s) 716
ApeKI GCWGC 8 cut(s) 500, 2039, 2042, 2077, 2307, 2919, 3230, 3375
ApoI RAATTY 9 cut(s) 387, 560, 1093, 1843, 1966, 2704, 3027, 3057, 3368
ArsI GACNNNNNNTTYG 4 cut(s) 560, 592, 663, 695
AsiGI ACCGGT 1 cut(s) 2166
Asp700I GAANNNNTTC 5 cut(s) 561, 761, 1086, 1901, 1966
AspLEI GCGC 4 cut(s) 717, 1036, 2205, 2827
AsuC2I CCSGG 5 cut(s) 1325, 1326, 1448, 2051, 2635
AsuHPI GGTGA 5 cut(s) 755, 940, 1852, 2443, 2627
AsuII TTCGAA 2 cut(s) 262, 1970
AsuNHI GCTAGC 1 cut(s) 941
AvaI CYCGRG 3 cut(s) 718, 1324, 2252
AvaII GGWCC 8 cut(s) 274, 902, 1040, 1718, 2020, 2169, 2389, 2493
BanI GGYRCC 1 cut(s) 3176
BanII GRGCYC 5 cut(s) 339, 540, 1149, 2520, 2737
BarI GAAGNNNNNNTAC 4 cut(s) 120, 152, 2330, 2362
BauI CACGAG 2 cut(s) 1361, 2580
BbrPI CACGTG 1 cut(s) 3268
BbsI GAAGAC 1 cut(s) 1383
Bbv12I GWGCWC 3 cut(s) 540, 1149, 2877
BbvI GCAGC 8 cut(s) 487, 2026, 2054, 2064, 2319, 2906, 3242, 3387
BccI CCATC 6 cut(s) 461, 982, 1040, 1594, 2657, 2999
BceAI ACGGC 5 cut(s) 138, 1346, 2352, 2380, 3178
BcnI CCSGG 5 cut(s) 1325, 1326, 1448, 2051, 2635
BcoDI GTCTC 9 cut(s) 256, 582, 837, 1277, 1594, 1645, 2662, 2858, 3281
BfmI CTRYAG 2 cut(s) 1005, 2040
BfoI RGCGCY 2 cut(s) 718, 1037
BfrI CTTAAG 2 cut(s) 1385, 1796
BglI GCCNNNNNGGC 1 cut(s) 1364
Bme18I GGWCC 8 cut(s) 274, 902, 1040, 1718, 2020, 2169, 2389, 2493
BmeRI GACNNNNNGTC 1 cut(s) 673
BmeT110I CYCGRG 3 cut(s) 718, 1324, 2252
BmgBI CACGTC 1 cut(s) 2713
BmrI ACTGGG 6 cut(s) 242, 436, 922, 1692, 1896, 3031
BmsI GCATC 7 cut(s) 38, 954, 1315, 1677, 2260, 2957, 3341
BmtI GCTAGC 1 cut(s) 945
BmuI ACTGGG 6 cut(s) 242, 436, 922, 1692, 1896, 3031
BpiI GAAGAC 1 cut(s) 1383
BplI GAGNNNNNCTC 2 cut(s) 522, 554
BpmI CTGGAG 5 cut(s) 524, 599, 2422, 2749, 3364
Bpu10I CCTNAGC 1 cut(s) 1691
Bpu14I TTCGAA 2 cut(s) 262, 1970
BpuEI CTTGAG 4 cut(s) 650, 1259, 1487, 1989
BpuMI CCSGG 5 cut(s) 1325, 1326, 1448, 2051, 2635
Bsa29I ATCGAT 1 cut(s) 2478
BsaAI YACGTR 3 cut(s) 2796, 2838, 3268
BsaBI GATNNNNATC 1 cut(s) 3009
BsaHI GRCGYC 1 cut(s) 1826
BsaI GGTCTC 1 cut(s) 837
BsaWI WCCGGW 4 cut(s) 1042, 1720, 2166, 2414
BsaXI ACNNNNNCTCC 2 cut(s) 2864, 2894
Bse118I RCCGGY 2 cut(s) 2166, 2599
Bse3DI GCAATG 1 cut(s) 2192
Bse8I GATNNNNATC 1 cut(s) 3009
BseAI TCCGGA 3 cut(s) 1042, 1720, 2414
BseCI ATCGAT 1 cut(s) 2478
BseGI GGATG 8 cut(s) 29, 456, 1051, 1173, 1692, 1911, 2948, 3393
BseJI GATNNNNATC 1 cut(s) 3009
BseMI GCAATG 1 cut(s) 2192
BseMII CTCAG 4 cut(s) 596, 1589, 2853, 3291
BseRI GAGGAG 5 cut(s) 548, 875, 1767, 3096, 3247
BseX3I CGGCCG 2 cut(s) 1921, 2365
BseXI GCAGC 8 cut(s) 487, 2026, 2054, 2064, 2319, 2906, 3242, 3387
BseYI CCCAGC 2 cut(s) 1411, 1979
BsgI GTGCAG 2 cut(s) 3249, 3290
Bsh1236I CGCG 4 cut(s) 2827, 2902, 3153, 3365
Bsh1285I CGRYCG 4 cut(s) 1447, 1924, 2323, 2368
BshNI GGYRCC 1 cut(s) 3176
BshTI ACCGGT 1 cut(s) 2166
BshVI ATCGAT 1 cut(s) 2478
BsiEI CGRYCG 4 cut(s) 1447, 1924, 2323, 2368
BsiHKAI GWGCWC 3 cut(s) 540, 1149, 2877
BsiHKCI CYCGRG 3 cut(s) 718, 1324, 2252
BslFI GGGAC 9 cut(s) 680, 1307, 1341, 1428, 1560, 1903, 1970, 2155, 3032
BsmAI GTCTC 9 cut(s) 256, 582, 837, 1277, 1594, 1645, 2662, 2858, 3281
BsmBI CGTCTC 2 cut(s) 1277, 2662
BsmFI GGGAC 9 cut(s) 680, 1307, 1341, 1428, 1560, 1903, 1970, 2155, 3032
BsmI GAATGC 3 cut(s) 1318, 1817, 2218
Bso31I GGTCTC 1 cut(s) 837
BsoBI CYCGRG 3 cut(s) 718, 1324, 2252
Bsp119I TTCGAA 2 cut(s) 262, 1970
Bsp1286I GDGCHC 6 cut(s) 339, 540, 1149, 2520, 2737, 2877
Bsp13I TCCGGA 3 cut(s) 1042, 1720, 2414
Bsp68I TCGCGA 1 cut(s) 3153
BspCNI CTCAG 4 cut(s) 597, 1590, 2854, 3292
BspDI ATCGAT 1 cut(s) 2478
BspEI TCCGGA 3 cut(s) 1042, 1720, 2414
BspFNI CGCG 4 cut(s) 2827, 2902, 3153, 3365
BspMAI CTGCAG 2 cut(s) 1009, 2044
BspOI GCTAGC 1 cut(s) 945
BspPI GGATC 3 cut(s) 165, 971, 1604
BspT104I TTCGAA 2 cut(s) 262, 1970
BspT107I GGYRCC 1 cut(s) 3176
BspTI CTTAAG 2 cut(s) 1385, 1796
BspTNI GGTCTC 1 cut(s) 837
BsrBI CCGCTC 2 cut(s) 1926, 3143
BsrDI GCAATG 1 cut(s) 2192
BsrFI RCCGGY 2 cut(s) 2166, 2599
BssAI RCCGGY 2 cut(s) 2166, 2599
BssNI GRCGYC 1 cut(s) 1826
BssSI CACGAG 2 cut(s) 1361, 2580
BssT1I CCWWGG 4 cut(s) 140, 146, 1915, 1941
Bst2BI CACGAG 2 cut(s) 1361, 2580
Bst4CI ACNGT 4 cut(s) 278, 1502, 2626, 2869
Bst6I CTCTTC 2 cut(s) 1067, 3041
BstACI GRCGYC 1 cut(s) 1826
BstAFI CTTAAG 2 cut(s) 1385, 1796
BstAPI GCANNNNNTGC 1 cut(s) 56
BstBAI YACGTR 3 cut(s) 2796, 2838, 3268
BstBI TTCGAA 2 cut(s) 262, 1970
BstC8I GCNNGC 8 cut(s) 399, 943, 2082, 2242, 2289, 2601, 2772, 2904
BstDEI CTNAG 9 cut(s) 605, 1126, 1598, 1691, 1929, 2274, 2862, 2987, 3300
BstDSI CCRYGG 2 cut(s) 77, 277
BstEII GGTNACC 1 cut(s) 881
BstF5I GGATG 8 cut(s) 29, 456, 1051, 1173, 1692, 1911, 2948, 3393
BstFNI CGCG 4 cut(s) 2827, 2902, 3153, 3365
BstH2I RGCGCY 2 cut(s) 718, 1037
BstHHI GCGC 4 cut(s) 717, 1036, 2205, 2827
BstMAI GTCTC 9 cut(s) 256, 582, 837, 1277, 1594, 1645, 2662, 2858, 3281
BstMCI CGRYCG 4 cut(s) 1447, 1924, 2323, 2368
BstNSI RCATGY 1 cut(s) 332
BstPI GGTNACC 1 cut(s) 881
BstSFI CTRYAG 2 cut(s) 1005, 2040
BstUI CGCG 4 cut(s) 2827, 2902, 3153, 3365
BstV1I GCAGC 8 cut(s) 487, 2026, 2054, 2064, 2319, 2906, 3242, 3387
BstV2I GAAGAC 1 cut(s) 1383
BstX2I RGATCY 1 cut(s) 1609
BstYI RGATCY 1 cut(s) 1609
BstZI CGGCCG 2 cut(s) 1921, 2365
Bsu15I ATCGAT 1 cut(s) 2478
BsuTUI ATCGAT 1 cut(s) 2478
BtgI CCRYGG 2 cut(s) 77, 277
BtrI CACGTC 1 cut(s) 2713
BtsCI GGATG 8 cut(s) 29, 456, 1051, 1173, 1692, 1911, 2948, 3393
BtsI GCAGTG 1 cut(s) 2706
BtsIMutI CAGTG 5 cut(s) 1878, 2106, 2379, 2706, 2960
BtuMI TCGCGA 1 cut(s) 3153
Cac8I GCNNGC 8 cut(s) 399, 943, 2082, 2242, 2289, 2601, 2772, 2904
CaiI CAGNNNCTG 2 cut(s) 2496, 3335
CciNI GCGGCCGC 1 cut(s) 1921
CfoI GCGC 4 cut(s) 717, 1036, 2205, 2827
Cfr10I RCCGGY 2 cut(s) 2166, 2599
Cfr9I CCCGGG 1 cut(s) 1324
ClaI ATCGAT 1 cut(s) 2478
CseI GACGC 5 cut(s) 755, 1815, 2280, 2908, 3375
Csp6I GTAC 3 cut(s) 156, 1864, 2638
CspAI ACCGGT 1 cut(s) 2166
CviQI GTAC 3 cut(s) 156, 1864, 2638
DdeI CTNAG 9 cut(s) 605, 1126, 1598, 1691, 1929, 2274, 2862, 2987, 3300
DraI TTTAAA 1 cut(s) 2235
DriI GACNNNNNGTC 1 cut(s) 673
EaeI YGGCCR 5 cut(s) 311, 1921, 2365, 2466, 2597
EagI CGGCCG 2 cut(s) 1921, 2365
Eam1104I CTCTTC 2 cut(s) 1067, 3041
Eam1105I GACNNNNNGTC 1 cut(s) 673
EarI CTCTTC 2 cut(s) 1067, 3041
Ecl136II GAGCTC 2 cut(s) 538, 1147
EclXI CGGCCG 2 cut(s) 1921, 2365
Eco130I CCWWGG 4 cut(s) 140, 146, 1915, 1941
Eco147I AGGCCT 1 cut(s) 1940
Eco24I GRGCYC 5 cut(s) 339, 540, 1149, 2520, 2737
Eco31I GGTCTC 1 cut(s) 837
Eco32I GATATC 3 cut(s) 164, 2329, 3094
Eco47I GGWCC 8 cut(s) 274, 902, 1040, 1718, 2020, 2169, 2389, 2493
Eco47III AGCGCT 1 cut(s) 716
Eco52I CGGCCG 2 cut(s) 1921, 2365
Eco53kI GAGCTC 2 cut(s) 538, 1147
Eco72I CACGTG 1 cut(s) 3268
Eco88I CYCGRG 3 cut(s) 718, 1324, 2252
Eco91I GGTNACC 1 cut(s) 881
EcoICRI GAGCTC 2 cut(s) 538, 1147
EcoO109I RGGNCCY 4 cut(s) 902, 1594, 2020, 2493
EcoO65I GGTNACC 1 cut(s) 881
EcoRI GAATTC 2 cut(s) 2704, 3368
EcoRV GATATC 3 cut(s) 164, 2329, 3094
EcoT14I CCWWGG 4 cut(s) 140, 146, 1915, 1941
EcoT38I GRGCYC 5 cut(s) 339, 540, 1149, 2520, 2737
ErhI CCWWGG 4 cut(s) 140, 146, 1915, 1941
Esp3I CGTCTC 2 cut(s) 1277, 2662
FalI AAGNNNNNCTT 6 cut(s) 1388, 1420, 1475, 1507, 2980, 3012
FaqI GGGAC 9 cut(s) 680, 1307, 1341, 1428, 1560, 1903, 1970, 2155, 3032
FauI CCCGC 3 cut(s) 733, 2249, 2895
FblI GTMKAC 1 cut(s) 66
FokI GGATG 8 cut(s) 16, 443, 1058, 1160, 1699, 1898, 2935, 3380
FriOI GRGCYC 5 cut(s) 339, 540, 1149, 2520, 2737
FspI TGCGCA 1 cut(s) 2204
GlaI GCGC 4 cut(s) 716, 1035, 2204, 2826
GsaI CCCAGC 2 cut(s) 1415, 1983
GsuI CTGGAG 5 cut(s) 524, 599, 2422, 2749, 3364
HaeII RGCGCY 2 cut(s) 718, 1037
HgaI GACGC 5 cut(s) 755, 1815, 2280, 2908, 3375
HhaI GCGC 4 cut(s) 717, 1036, 2205, 2827
Hin1I GRCGYC 1 cut(s) 1826
Hin6I GCGC 4 cut(s) 715, 1034, 2203, 2825
HinP1I GCGC 4 cut(s) 715, 1034, 2203, 2825
HincII GTYRAC 4 cut(s) 67, 972, 2778, 3188
HindII GTYRAC 4 cut(s) 67, 972, 2778, 3188
HindIII AAGCTT 1 cut(s) 420
HpaI GTTAAC 2 cut(s) 972, 2778
HphI GGTGA 5 cut(s) 755, 940, 1852, 2443, 2627
Hpy166II GTNNAC 5 cut(s) 67, 972, 1204, 2778, 3188
Hpy8I GTNNAC 5 cut(s) 67, 972, 1204, 2778, 3188
Hpy99I CGWCG 5 cut(s) 122, 214, 1201, 1831, 2714
HpyCH4III ACNGT 4 cut(s) 278, 1502, 2626, 2869
HpyCH4IV ACGT 6 cut(s) 1196, 2655, 2712, 2795, 2837, 3267
HpyF3I CTNAG 9 cut(s) 605, 1126, 1598, 1691, 1929, 2274, 2862, 2987, 3300
HpySE526I ACGT 6 cut(s) 1196, 2655, 2712, 2795, 2837, 3267
Hsp92I GRCGYC 1 cut(s) 1826
HspAI GCGC 4 cut(s) 715, 1034, 2203, 2825
Kpn2I TCCGGA 3 cut(s) 1042, 1720, 2414
KroI GCCGGC 1 cut(s) 2599
KroNI GCCGGC 1 cut(s) 2601
KspAI GTTAAC 2 cut(s) 972, 2778
Lsp1109I GCAGC 8 cut(s) 487, 2026, 2054, 2064, 2319, 2906, 3242, 3387
LweI GCATC 7 cut(s) 38, 954, 1315, 1677, 2260, 2957, 3341
MaeII ACGT 6 cut(s) 1196, 2655, 2712, 2795, 2837, 3267
MbiI CCGCTC 2 cut(s) 1926, 3143
MboII GAAGA 9 cut(s) 158, 484, 1054, 1383, 1625, 1984, 2679, 2826, 3028
MfeI CAATTG 1 cut(s) 3196
MflI RGATCY 1 cut(s) 1609
MhlI GDGCHC 6 cut(s) 339, 540, 1149, 2520, 2737, 2877
MlyI GAGTC 2 cut(s) 62, 1745
MmeI TCCRAC 1 cut(s) 2733
MroI TCCGGA 3 cut(s) 1042, 1720, 2414
MroNI GCCGGC 1 cut(s) 2599
MroXI GAANNNNTTC 5 cut(s) 561, 761, 1086, 1901, 1966
MslI CAYNNNNRTG 4 cut(s) 263, 444, 1311, 2480
MspA1I CMGCKG 1 cut(s) 2394
MspCI CTTAAG 2 cut(s) 1385, 1796
MunI CAATTG 1 cut(s) 3196
Mva1269I GAATGC 3 cut(s) 1318, 1817, 2218
MvnI CGCG 4 cut(s) 2827, 2902, 3153, 3365
NaeI GCCGGC 1 cut(s) 2601
NciI CCSGG 5 cut(s) 1325, 1326, 1448, 2051, 2635
NgoMIV GCCGGC 1 cut(s) 2599
NheI GCTAGC 1 cut(s) 941
NmeAIII GCCGAG 2 cut(s) 1769, 1802
NmuCI GTSAC 4 cut(s) 743, 2582, 2615, 3401
NotI GCGGCCGC 1 cut(s) 1921
NruI TCGCGA 1 cut(s) 3153
NsbI TGCGCA 1 cut(s) 2204
NspI RCATGY 1 cut(s) 332
NspV TTCGAA 2 cut(s) 262, 1970
OliI CACNNNNGTG 1 cut(s) 444
PaeR7I CTCGAG 1 cut(s) 718
PasI CCCWGGG 1 cut(s) 339
PceI AGGCCT 1 cut(s) 1940
PcsI WCGNNNNNNNCGW 4 cut(s) 166, 675, 790, 1449
PctI GAATGC 3 cut(s) 1318, 1817, 2218
PdiI GCCGGC 1 cut(s) 2601
PdmI GAANNNNTTC 5 cut(s) 561, 761, 1086, 1901, 1966
PflFI GACNNNGTC 2 cut(s) 248, 1448
PfoI TCCNGGA 1 cut(s) 2495
PinAI ACCGGT 1 cut(s) 2166
PleI GAGTC 2 cut(s) 62, 1745
PmaCI CACGTG 1 cut(s) 3268
PmlI CACGTG 1 cut(s) 3268
PpsI GAGTC 2 cut(s) 62, 1745
Ppu21I YACGTR 3 cut(s) 2796, 2838, 3268
PpuMI RGGWCCY 3 cut(s) 902, 2020, 2493
Psp124BI GAGCTC 2 cut(s) 540, 1149
Psp5II RGGWCCY 3 cut(s) 902, 2020, 2493
PspCI CACGTG 1 cut(s) 3268
PspEI GGTNACC 1 cut(s) 881
PspFI CCCAGC 2 cut(s) 1411, 1979
PspPPI RGGWCCY 3 cut(s) 902, 2020, 2493
PspXI VCTCGAGB 1 cut(s) 718
PstI CTGCAG 2 cut(s) 1009, 2044
PstNI CAGNNNCTG 2 cut(s) 2496, 3335
PsuI RGATCY 1 cut(s) 1609
PsyI GACNNNGTC 2 cut(s) 248, 1448
RruI TCGCGA 1 cut(s) 3153
RsaI GTAC 3 cut(s) 157, 1865, 2639
RsaNI GTAC 3 cut(s) 156, 1864, 2638
RseI CAYNNNNRTG 4 cut(s) 263, 444, 1311, 2480
SacI GAGCTC 2 cut(s) 540, 1149
SalI GTCGAC 1 cut(s) 65
SchI GAGTC 2 cut(s) 62, 1745
SduI GDGCHC 6 cut(s) 339, 540, 1149, 2520, 2737, 2877
SfaNI GCATC 7 cut(s) 38, 954, 1315, 1677, 2260, 2957, 3341
SfcI CTRYAG 2 cut(s) 1005, 2040
Sfr274I CTCGAG 1 cut(s) 718
SfuI TTCGAA 2 cut(s) 262, 1970
SinI GGWCC 8 cut(s) 274, 902, 1040, 1718, 2020, 2169, 2389, 2493
SlaI CTCGAG 1 cut(s) 718
SmaI CCCGGG 1 cut(s) 1326
SmiMI CAYNNNNRTG 4 cut(s) 263, 444, 1311, 2480
SmlI CTYRAG 7 cut(s) 629, 718, 1274, 1385, 1466, 1796, 2004
SmoI CTYRAG 7 cut(s) 629, 718, 1274, 1385, 1466, 1796, 2004
SseBI AGGCCT 1 cut(s) 1940
SspI AATATT 2 cut(s) 1348, 1663
SstI GAGCTC 2 cut(s) 540, 1149
StuI AGGCCT 1 cut(s) 1940
StyI CCWWGG 4 cut(s) 140, 146, 1915, 1941
TaaI ACNGT 4 cut(s) 278, 1502, 2626, 2869
TaiI ACGT 6 cut(s) 1199, 2658, 2715, 2798, 2840, 3270
TaqII GACCGA 1 cut(s) 2309
TatI WGTACW 1 cut(s) 1863
TauI GCSGC 6 cut(s) 135, 1923, 1926, 2844, 3182, 3212
TscAI CASTG 5 cut(s) 1885, 2113, 2379, 2706, 2967
TseFI GTSAC 4 cut(s) 743, 2582, 2615, 3401
TseI GCWGC 8 cut(s) 500, 2039, 2042, 2077, 2307, 2919, 3230, 3375
Tsp45I GTSAC 4 cut(s) 743, 2582, 2615, 3401
TspDTI ATGAA 9 cut(s) 113, 281, 450, 611, 926, 1445, 3028, 3160, 3382
TspGWI ACGGA 2 cut(s) 667, 1370
TspMI CCCGGG 1 cut(s) 1324
TspRI CASTG 5 cut(s) 1885, 2113, 2379, 2706, 2967
Tth111I GACNNNGTC 2 cut(s) 248, 1448
Vha464I CTTAAG 2 cut(s) 1385, 1796
VpaK11BI GGWCC 8 cut(s) 274, 902, 1040, 1718, 2020, 2169, 2389, 2493
XapI RAATTY 9 cut(s) 387, 560, 1093, 1843, 1966, 2704, 3027, 3057, 3368
XceI RCATGY 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 2959
XhoI CTCGAG 1 cut(s) 718
XmaI CCCGGG 1 cut(s) 1324
XmiI GTMKAC 1 cut(s) 66
XmnI GAANNNNTTC 5 cut(s) 561, 761, 1086, 1901, 1966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.