Rw1G021220

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000194
Physical Location & Seq
Reverse (-)
45352407 .. 45354950
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021220.1

Sequence Viewer

Length: 2280 bp
ATGCCAACTCTCATCTCTGACAACCAATCTGCTTTTGTTCCTGGACGACAAATACAGGACAACATTTTGGTTGCTCACGAACTCTTTCACCATCTAAAGCTTCTCAAATCAACCAATAGTACTGGTGAATTTGCTCTCAAATTGGACATGAATAAAGCTTATGATCGTGTTGATTGGAACTTCCTACAACAAGTCCTTCTCAAGATGGGATTCCACCCAACTTGGGTCCGTATGGTTATGGCTTGTGTTACATCTATTTCGTACTCTCTTTTGATCAATTGTTCACCGGGCCGGTGGTTCAAACCGACAAGGGGATTAAGGCAGGGCGACCCCCTCTTTCCTTTCCTCTTTTTATTTGTCAATGATGTTTTGTCTTCTATGATCAGGAAAATTTGTGATGCCAACTTACTGGATGCGGTTAAGTTGAACCCTGACGGGCCCTTGGTTAGTCATTTATTCTTTGCTGATGATTCGATCTTTTTCCTCAAGGCCACTCTTTACCATTGTGAGGCATTGTCGGATACTCTACACCATTATTGTAGTGCATCAGGTCAATCCATCAACTTGGAAAAATCATCACTCTTCTTTAGCCCAAACACATCTCCTGAGATTGTAGACCTCCTCGGCGCTATCATGGGAATTCGTACGGTTCTGAATCCGGGCTCGTATCTGGGTCTTCCGACCATTTGGGGAAGGTCCAAAGTCTCTGCTCTTACTTATGTTAAAGATAATTTATCCAAAAAGATTTTGGGTTGGAACAATTCACATCTTACACAGGCTGGGAAAGAAATTTTGATCAAATCTGTGGCTATGGCGGTTCCGGCTTACCCGATGTCGGTGTTCAAATTTCCGAACACACTATGTAACAAGATCAATTCTGTTCTGGCAAACTTTTGGTGGGGTAGTTCCTCCTCCTCGGGAATCCATTGGAAAAACTGGGGTTCTCTGTCACTCCTGAAATCCGAAGGAGGGATGGGATTCCGTGGTTTAGCTGATTTCAACCTTGCTCTCTTGGCCAAACAGGGTTGGCGTATTCTCTCCAACCCGAATGCTTTGTGGGTGTCTGTGCTTAAGAGCCGCTATTTTCCCCGGTGCTCATTTCTTGAAGCTACACGGGGATCCTCCCCTTCCTGGGCCTGGTCTAGCATCCTTGCAGGCCGTGAATTACTTTGTGCAGGTTCCATTTGGCAGATTGGCTCGGGCCATAATATTGATTTCTGGCGGGATAACTGGATACAGAGGTACCCTTCTTTCAACCTTCTATCTCACATGCCTACTGATGCACCATTTGTCTCAGTACATCAGGTTATTGACAGACGAAATGATTCATGGAACCTGGGTTCTTTATCTAACAATCTGCCGGCTGCAGCAATTGTTGCAATCAGGGCAACCCCTTTTGGCTCCCTGAACGATATTGACAAACTTATTTGGTCAGCTACTACTACGGGAGCTTATTCTGTTAAATCTGGTTACAAGCTCAAAATTTCGACTCAAACCCCACTTCGCTCCATACATCCTTTTCACTCCCACTCCATTAATGAGAAGGTTTGGAAATGGCTGTGGGCGGTCAGGGCCACTCCCAGAATTAAGCATTTTCTTTGGCGGGCTATTTCTAGTGCTCTGGCAACAAATGATAACCGACACCGAAGGCATCTATGTAATTCCCCTATGTGCCTTATCTGTGATGCACATCCAGAAACCATTGAACATGTCTTATTTCTTTGCCCGTGGGTGACTAGAGCTTGGTTTTCTCACCCTCTTTCCTACAGAATTGATCTTCAAGCTATTACATCCTTGGATAGATGGATTCTTGCTCTGTTTGGTGATCATCTTGCAGTTCAGGATTTCCTTGTTTGTGGTTTGGCAGCTATTCTTCGAGATTCTACAGGGACCTTAATTGGCGGCTCATCTCAAAGAATGCTAGCCTCCTCCTCTATGGCTGCTGAAGCTTTGGCTATCAACCTTGGCCTCAGTTTAGCCTCCTCTCTCTCTCTTTCTTCTTTTCAGTTGGAATCCGACTCTCTGGTTCTGATTTCAGCCTTTAAAAACCCCCACTCGGCGGTGGATTGGTCGGCTTCTCAGATTGTATCTCGCACTCGTGTTAATGCCTATCAGTTTACTCGTGTTAACTGGCTTTGGACCAGTAGGAATGCAAATGCTGCTGCAGACTGTGTGGCAGCTTTGGCATCTCGCAGGGTGTGTCCGGCGGATTGGGTCACCAACCCTCCTACCTCCCTCATGCGTATTCTGCTCTTTGACGCGGCTGGCCTTCCTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

759

Amino Acids

84.52

Weight (kDa)

9.38

Isoelectric Point (pI)

36.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 4 - 209 3.2e-19 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 508 - 582 2.3e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 620 - 728 4.4e-13 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1166
Acc65I GGTACC 1 cut(s) 1242
AccB1I GGYRCC 1 cut(s) 1242
AccI GTMKAC 1 cut(s) 615
AccII CGCG 1 cut(s) 2261
AclWI GGATC 2 cut(s) 1113, 1126
AcoI YGGCCR 1 cut(s) 1014
AcsI RAATTY 6 cut(s) 128, 390, 639, 789, 845, 1482
AcuI CTGAAG 1 cut(s) 1965
AfaI GTAC 5 cut(s) 121, 263, 646, 1244, 1299
AflII CTTAAG 1 cut(s) 1070
AflIII ACRYGT 1 cut(s) 1708
AgsI TTSAA 8 cut(s) 301, 427, 844, 1000, 1106, 1255, 1706, 1781
AjnI CCWGG 4 cut(s) 40, 1130, 1136, 1335
AloI GAACNNNNNNTCC 2 cut(s) 1324, 1356
Alw21I GWGCWC 2 cut(s) 1097, 1621
Alw26I GTCTC 2 cut(s) 709, 1297
AlwI GGATC 2 cut(s) 1113, 1126
Ama87I CYCGRG 2 cut(s) 916, 1198
ApaI GGGCCC 1 cut(s) 441
ApeKI GCWGC 7 cut(s) 1364, 1367, 1865, 1940, 2159, 2162, 2177
ApoI RAATTY 6 cut(s) 128, 390, 639, 789, 845, 1482
AseI ATTAAT 1 cut(s) 1536
Asp700I GAANNNNTTC 2 cut(s) 84, 1324
Asp718I GGTACC 1 cut(s) 1242
AspLEI GCGC 1 cut(s) 629
AsuC2I CCSGG 3 cut(s) 288, 660, 1090
AsuHPI GGTGA 7 cut(s) 80, 137, 276, 1744, 1745, 1835, 2209
AsuNHI GCTAGC 1 cut(s) 1921
AvaI CYCGRG 2 cut(s) 916, 1198
AvaII GGWCC 4 cut(s) 226, 696, 1890, 2139
BaeGI GKGCMC 1 cut(s) 441
BaeI ACNNNNGTAYC 4 cut(s) 1226, 1226, 1259, 1259
BalI TGGCCA 1 cut(s) 1016
BamHI GGATCC 1 cut(s) 1118
BanI GGYRCC 1 cut(s) 1242
BanII GRGCYC 2 cut(s) 441, 665
BauI CACGAG 2 cut(s) 2097, 2121
BbsI GAAGAC 2 cut(s) 366, 668
Bbv12I GWGCWC 2 cut(s) 1097, 1621
BbvI GCAGC 7 cut(s) 1351, 1379, 1877, 1927, 2146, 2149, 2189
BccI CCATC 5 cut(s) 99, 199, 566, 967, 1797
BceAI ACGGC 1 cut(s) 1143
BciT130I CCWGG 4 cut(s) 42, 1132, 1138, 1337
BciVI GTATCC 2 cut(s) 514, 1227
BclI TGATCA 4 cut(s) 273, 381, 795, 1825
BcnI CCSGG 3 cut(s) 288, 660, 1090
BcoDI GTCTC 2 cut(s) 709, 1297
BfaI CTAG 4 cut(s) 1143, 1614, 1737, 1922
BfmI CTRYAG 4 cut(s) 1365, 1765, 1884, 2163
BfoI RGCGCY 1 cut(s) 630
BfrI CTTAAG 1 cut(s) 1070
BfuAI ACCTGC 1 cut(s) 1166
BfuI GTATCC 2 cut(s) 514, 1227
BmcAI AGTACT 1 cut(s) 121
Bme1390I CCNGG 7 cut(s) 42, 288, 660, 1090, 1132, 1138, 1337
Bme18I GGWCC 4 cut(s) 226, 696, 1890, 2139
BmeT110I CYCGRG 2 cut(s) 916, 1198
BmiI GGNNCC 9 cut(s) 227, 439, 819, 1120, 1180, 1244, 1334, 1402, 1891
BmrFI CCNGG 7 cut(s) 42, 288, 660, 1090, 1132, 1138, 1337
BmrI ACTGGG 1 cut(s) 946
BmsI GCATC 8 cut(s) 388, 403, 554, 1155, 1270, 1660, 1675, 2195
BmtI GCTAGC 1 cut(s) 1925
BmuI ACTGGG 1 cut(s) 946
BpiI GAAGAC 2 cut(s) 366, 668
BpuEI CTTGAG 2 cut(s) 185, 470
BpuMI CCSGG 3 cut(s) 288, 660, 1090
BsaBI GATNNNNATC 1 cut(s) 1689
BsaXI ACNNNNNCTCC 4 cut(s) 1514, 1544, 2209, 2239
Bse118I RCCGGY 2 cut(s) 291, 1360
Bse1I ACTGG 6 cut(s) 127, 414, 941, 1235, 2135, 2142
Bse8I GATNNNNATC 1 cut(s) 1689
BseBI CCWGG 4 cut(s) 42, 1132, 1138, 1337
BseGI GGATG 6 cut(s) 418, 978, 1146, 1513, 1690, 1790
BseJI GATNNNNATC 1 cut(s) 1689
BseMII CTCAG 4 cut(s) 597, 1308, 1984, 2093
BseNI ACTGG 6 cut(s) 127, 414, 941, 1235, 2135, 2142
BseRI GAGGAG 6 cut(s) 611, 901, 904, 1918, 1921, 1972
BseSI GKGCMC 1 cut(s) 441
BseXI GCAGC 7 cut(s) 1351, 1379, 1877, 1927, 2146, 2149, 2189
BseYI CCCAGC 1 cut(s) 779
BsgI GTGCAG 1 cut(s) 1194
Bsh1236I CGCG 1 cut(s) 2261
BshNI GGYRCC 1 cut(s) 1242
BsiHKAI GWGCWC 2 cut(s) 1097, 1621
BsiHKCI CYCGRG 2 cut(s) 916, 1198
BsiSI CCGG 7 cut(s) 287, 292, 659, 821, 1090, 1361, 2204
BsiWI CGTACG 1 cut(s) 644
BslFI GGGAC 1 cut(s) 1903
BsmAI GTCTC 2 cut(s) 709, 1297
BsmFI GGGAC 1 cut(s) 1903
BsmI GAATGC 3 cut(s) 1054, 1923, 2155
BsoBI CYCGRG 2 cut(s) 916, 1198
Bsp120I GGGCCC 1 cut(s) 437
Bsp1286I GDGCHC 4 cut(s) 441, 665, 1097, 1621
Bsp143I GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
BspCNI CTCAG 4 cut(s) 598, 1307, 1983, 2092
BspFNI CGCG 1 cut(s) 2261
BspLI GGNNCC 9 cut(s) 227, 439, 819, 1120, 1180, 1244, 1334, 1402, 1891
BspMAI CTGCAG 2 cut(s) 1369, 2167
BspMI ACCTGC 1 cut(s) 1166
BspOI GCTAGC 1 cut(s) 1925
BspPI GGATC 2 cut(s) 1113, 1126
BspT107I GGYRCC 1 cut(s) 1242
BspTI CTTAAG 1 cut(s) 1070
BsrFI RCCGGY 2 cut(s) 291, 1360
BsrI ACTGG 6 cut(s) 127, 414, 941, 1235, 2135, 2142
BssAI RCCGGY 2 cut(s) 291, 1360
BssMI GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
BssSI CACGAG 2 cut(s) 2097, 2121
BssT1I CCWWGG 3 cut(s) 441, 1794, 1963
Bst2BI CACGAG 2 cut(s) 2097, 2121
Bst2UI CCWGG 4 cut(s) 42, 1132, 1138, 1337
Bst4CI ACNGT 2 cut(s) 649, 2171
Bst6I CTCTTC 1 cut(s) 587
BstAFI CTTAAG 1 cut(s) 1070
BstAPI GCANNNNNTGC 2 cut(s) 1376, 2159
BstC8I GCNNGC 5 cut(s) 1156, 1362, 1605, 1923, 2266
BstDEI CTNAG 4 cut(s) 606, 1294, 1970, 2079
BstDSI CCRYGG 2 cut(s) 982, 1727
BstEII GGTNACC 1 cut(s) 2215
BstF5I GGATG 6 cut(s) 418, 978, 1146, 1513, 1690, 1790
BstFNI CGCG 1 cut(s) 2261
BstH2I RGCGCY 1 cut(s) 630
BstHHI GCGC 1 cut(s) 629
BstKTI GATC 9 cut(s) 166, 276, 384, 477, 798, 873, 1121, 1777, 1828
BstMAI GTCTC 2 cut(s) 709, 1297
BstMBI GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
BstMWI GCNNNNNNNGC 9 cut(s) 821, 1013, 1376, 1385, 1571, 1946, 2159, 2183, 2248
BstNI CCWGG 4 cut(s) 42, 1132, 1138, 1337
BstNSI RCATGY 2 cut(s) 1273, 1712
BstPI GGTNACC 1 cut(s) 2215
BstSCI CCNGG 7 cut(s) 40, 286, 658, 1088, 1130, 1136, 1335
BstSFI CTRYAG 4 cut(s) 1365, 1765, 1884, 2163
BstSLI GKGCMC 1 cut(s) 441
BstUI CGCG 1 cut(s) 2261
BstV1I GCAGC 7 cut(s) 1351, 1379, 1877, 1927, 2146, 2149, 2189
BstV2I GAAGAC 2 cut(s) 366, 668
BstX2I RGATCY 1 cut(s) 1118
BstXI CCANNNNNNTGG 2 cut(s) 409, 565
BstYI RGATCY 1 cut(s) 1118
BsuI GTATCC 2 cut(s) 514, 1227
BtgI CCRYGG 2 cut(s) 982, 1727
BtsCI GGATG 6 cut(s) 418, 978, 1146, 1513, 1690, 1790
BveI ACCTGC 1 cut(s) 1166
Cac8I GCNNGC 5 cut(s) 1156, 1362, 1605, 1923, 2266
CfoI GCGC 1 cut(s) 629
Cfr10I RCCGGY 2 cut(s) 291, 1360
CseI GACGC 1 cut(s) 2267
Csp6I GTAC 5 cut(s) 120, 262, 645, 1243, 1298
CspCI CAANNNNNGTGG 2 cut(s) 203, 238
CviAII CATG 6 cut(s) 148, 634, 1270, 1329, 1709, 2239
CviQI GTAC 5 cut(s) 120, 262, 645, 1243, 1298
DdeI CTNAG 4 cut(s) 606, 1294, 1970, 2079
DpnI GATC 9 cut(s) 165, 275, 383, 476, 797, 872, 1120, 1776, 1827
DpnII GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
DraI TTTAAA 1 cut(s) 2044
EaeI YGGCCR 1 cut(s) 1014
Eam1104I CTCTTC 1 cut(s) 587
EarI CTCTTC 1 cut(s) 587
EciI GGCGGA 1 cut(s) 2222
Eco130I CCWWGG 3 cut(s) 441, 1794, 1963
Eco24I GRGCYC 2 cut(s) 441, 665
Eco47I GGWCC 4 cut(s) 226, 696, 1890, 2139
Eco57I CTGAAG 1 cut(s) 1965
Eco88I CYCGRG 2 cut(s) 916, 1198
Eco91I GGTNACC 1 cut(s) 2215
EcoO109I RGGNCCY 2 cut(s) 438, 1890
EcoO65I GGTNACC 1 cut(s) 2215
EcoRI GAATTC 1 cut(s) 639
EcoRII CCWGG 4 cut(s) 40, 1130, 1136, 1335
EcoT14I CCWWGG 3 cut(s) 441, 1794, 1963
EcoT38I GRGCYC 2 cut(s) 441, 665
ErhI CCWWGG 3 cut(s) 441, 1794, 1963
FaeI CATG 6 cut(s) 151, 637, 1273, 1332, 1712, 2242
FaqI GGGAC 1 cut(s) 1903
FatI CATG 6 cut(s) 147, 633, 1269, 1328, 1708, 2238
FauI CCCGC 2 cut(s) 1215, 1596
FbaI TGATCA 4 cut(s) 273, 381, 795, 1825
FblI GTMKAC 1 cut(s) 615
FokI GGATG 6 cut(s) 425, 985, 1133, 1500, 1677, 1777
FriOI GRGCYC 2 cut(s) 441, 665
FspBI CTAG 4 cut(s) 1143, 1614, 1737, 1922
GlaI GCGC 1 cut(s) 628
GsaI CCCAGC 1 cut(s) 783
HaeII RGCGCY 1 cut(s) 630
HapII CCGG 7 cut(s) 287, 292, 659, 821, 1090, 1361, 2204
HgaI GACGC 1 cut(s) 2267
HhaI GCGC 1 cut(s) 629
Hin1II CATG 6 cut(s) 151, 637, 1273, 1332, 1712, 2242
Hin6I GCGC 1 cut(s) 627
HinP1I GCGC 1 cut(s) 627
HincII GTYRAC 1 cut(s) 2128
HindII GTYRAC 1 cut(s) 2128
HindIII AAGCTT 3 cut(s) 98, 156, 1947
HpaI GTTAAC 1 cut(s) 2128
HpaII CCGG 7 cut(s) 287, 292, 659, 821, 1090, 1361, 2204
HphI GGTGA 7 cut(s) 80, 137, 276, 1744, 1745, 1835, 2209
Hpy166II GTNNAC 4 cut(s) 284, 616, 2118, 2128
Hpy188I TCNGA 9 cut(s) 19, 520, 654, 681, 852, 964, 2017, 2031, 2082
Hpy8I GTNNAC 4 cut(s) 284, 616, 2118, 2128
HpyAV CCTTC 9 cut(s) 206, 687, 959, 1137, 1257, 1268, 1537, 1641, 2279
HpyCH4III ACNGT 2 cut(s) 649, 2171
HpyF10VI GCNNNNNNNGC 9 cut(s) 821, 1013, 1376, 1385, 1571, 1946, 2159, 2183, 2248
HpyF3I CTNAG 4 cut(s) 606, 1294, 1970, 2079
Hsp92II CATG 6 cut(s) 151, 637, 1273, 1332, 1712, 2242
HspAI GCGC 1 cut(s) 627
KpnI GGTACC 1 cut(s) 1246
KroI GCCGGC 1 cut(s) 1360
KroNI GCCGGC 1 cut(s) 1362
Ksp22I TGATCA 4 cut(s) 273, 381, 795, 1825
KspAI GTTAAC 1 cut(s) 2128
Kzo9I GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
LmnI GCTCC 3 cut(s) 1406, 1448, 1511
Lsp1109I GCAGC 7 cut(s) 1351, 1379, 1877, 1927, 2146, 2149, 2189
LweI GCATC 8 cut(s) 388, 403, 554, 1155, 1270, 1660, 1675, 2195
MaeI CTAG 4 cut(s) 1143, 1614, 1737, 1922
MaeIII GTNAC 6 cut(s) 247, 863, 948, 1469, 1732, 2215
MalI GATC 9 cut(s) 165, 275, 383, 476, 797, 872, 1120, 1776, 1827
MboI GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
MboII GAAGA 6 cut(s) 366, 574, 668, 1769, 1865, 1989
MfeI CAATTG 2 cut(s) 277, 1371
MflI RGATCY 1 cut(s) 1118
MhlI GDGCHC 4 cut(s) 441, 665, 1097, 1621
MlsI TGGCCA 1 cut(s) 1016
MluNI TGGCCA 1 cut(s) 1016
MlyI GAGTC 2 cut(s) 1483, 2012
MmeI TCCRAC 6 cut(s) 498, 704, 734, 1065, 1989, 2040
Mox20I TGGCCA 1 cut(s) 1016
MroNI GCCGGC 1 cut(s) 1360
MroXI GAANNNNTTC 2 cut(s) 84, 1324
MscI TGGCCA 1 cut(s) 1016
Msp20I TGGCCA 1 cut(s) 1016
MspCI CTTAAG 1 cut(s) 1070
MspI CCGG 7 cut(s) 287, 292, 659, 821, 1090, 1361, 2204
MspR9I CCNGG 7 cut(s) 42, 288, 660, 1090, 1132, 1138, 1337
MunI CAATTG 2 cut(s) 277, 1371
Mva1269I GAATGC 3 cut(s) 1054, 1923, 2155
MvaI CCWGG 4 cut(s) 42, 1132, 1138, 1337
MvnI CGCG 1 cut(s) 2261
MwoI GCNNNNNNNGC 9 cut(s) 821, 1013, 1376, 1385, 1571, 1946, 2159, 2183, 2248
NaeI GCCGGC 1 cut(s) 1362
NciI CCSGG 3 cut(s) 288, 660, 1090
NdeII GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
NgoMIV GCCGGC 1 cut(s) 1360
NheI GCTAGC 1 cut(s) 1921
NlaIII CATG 6 cut(s) 151, 637, 1273, 1332, 1712, 2242
NlaIV GGNNCC 9 cut(s) 227, 439, 819, 1120, 1180, 1244, 1334, 1402, 1891
NmeAIII GCCGAG 2 cut(s) 603, 2036
NmuCI GTSAC 3 cut(s) 948, 1732, 2215
NspI RCATGY 2 cut(s) 1273, 1712
PciI ACATGT 1 cut(s) 1708
PctI GAATGC 3 cut(s) 1054, 1923, 2155
PdiI GCCGGC 1 cut(s) 1362
PdmI GAANNNNTTC 2 cut(s) 84, 1324
PfeI GAWTC 9 cut(s) 210, 470, 655, 921, 978, 1325, 1807, 1880, 2012
Pfl23II CGTACG 1 cut(s) 644
PfoI TCCNGGA 1 cut(s) 40
PleI GAGTC 2 cut(s) 1483, 2012
PpsI GAGTC 2 cut(s) 1483, 2012
PpuMI RGGWCCY 1 cut(s) 1890
PscI ACATGT 1 cut(s) 1708
PshBI ATTAAT 1 cut(s) 1536
Psp5II RGGWCCY 1 cut(s) 1890
Psp6I CCWGG 4 cut(s) 40, 1130, 1136, 1335
PspEI GGTNACC 1 cut(s) 2215
PspFI CCCAGC 1 cut(s) 779
PspGI CCWGG 4 cut(s) 40, 1130, 1136, 1335
PspLI CGTACG 1 cut(s) 644
PspN4I GGNNCC 9 cut(s) 227, 439, 819, 1120, 1180, 1244, 1334, 1402, 1891
PspOMI GGGCCC 1 cut(s) 437
PspPPI RGGWCCY 1 cut(s) 1890
PstI CTGCAG 2 cut(s) 1369, 2167
PsuI RGATCY 1 cut(s) 1118
RsaI GTAC 5 cut(s) 121, 263, 646, 1244, 1299
RsaNI GTAC 5 cut(s) 120, 262, 645, 1243, 1298
Sau3AI GATC 9 cut(s) 163, 273, 381, 474, 795, 870, 1118, 1774, 1825
ScaI AGTACT 1 cut(s) 121
SchI GAGTC 2 cut(s) 1483, 2012
ScrFI CCNGG 7 cut(s) 42, 288, 660, 1090, 1132, 1138, 1337
SduI GDGCHC 4 cut(s) 441, 665, 1097, 1621
SfaNI GCATC 8 cut(s) 388, 403, 554, 1155, 1270, 1660, 1675, 2195
SfcI CTRYAG 4 cut(s) 1365, 1765, 1884, 2163
SinI GGWCC 4 cut(s) 226, 696, 1890, 2139
SmlI CTYRAG 3 cut(s) 200, 485, 1070
SmoI CTYRAG 3 cut(s) 200, 485, 1070
SspI AATATT 1 cut(s) 1210
SspMI CTAG 4 cut(s) 1143, 1614, 1737, 1922
StyD4I CCNGG 7 cut(s) 40, 286, 658, 1088, 1130, 1136, 1335
StyI CCWWGG 3 cut(s) 441, 1794, 1963
TaaI ACNGT 2 cut(s) 649, 2171
TaqI TCGA 3 cut(s) 473, 1487, 1876
TatI WGTACW 2 cut(s) 119, 1297
TauI GCSGC 3 cut(s) 1080, 1905, 2264
TfiI GAWTC 9 cut(s) 210, 470, 655, 921, 978, 1325, 1807, 1880, 2012
TseFI GTSAC 3 cut(s) 948, 1732, 2215
TseI GCWGC 7 cut(s) 1364, 1367, 1865, 1940, 2159, 2162, 2177
Tsp45I GTSAC 3 cut(s) 948, 1732, 2215
TspDTI ATGAA 2 cut(s) 164, 1317
TspGWI ACGGA 2 cut(s) 218, 971
Vha464I CTTAAG 1 cut(s) 1070
VpaK11BI GGWCC 4 cut(s) 226, 696, 1890, 2139
VspI ATTAAT 1 cut(s) 1536
XapI RAATTY 6 cut(s) 128, 390, 639, 789, 845, 1482
XceI RCATGY 2 cut(s) 1273, 1712
XcmI CCANNNNNNNNNTGG 1 cut(s) 745
XmiI GTMKAC 1 cut(s) 615
XmnI GAANNNNTTC 2 cut(s) 84, 1324
XspI CTAG 4 cut(s) 1143, 1614, 1737, 1922
ZrmI AGTACT 1 cut(s) 121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.