Rorug07G0209000

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000194
Physical Location & Seq
Reverse (-)
18392164 .. 18396078
3915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0209000.1

Sequence Viewer

Length: 1137 bp
ATGTCTGTATTAATGGAGATGAGCTTGTTTCAATCGAAATCTATGTCTGCTTCTGAAATGGCTCAGGTGGGGAGGATGTACTCAAATTCGTTTACGAGTTTCAGGTACTTACCGGTTGGTATCAAAGTATTTCCACAGTTTTGTTCTTGCGCCTGCACAGGGAATCCTCTGAAGGCTTCTTCATTTTCTCATCCTTCTAGTTCAAACAATAGATATCTGTCAACAAACGCCATTGGTAGTGTTGGAGGGGATAAACTTGCAGCTGAAACTTCCTTGTTTCAAATCTTTGGAACAAATGGTGCAAAGTCAAATCTGTTTCCTAGAGACATTAGAGTACTTGATGCCTTTGATGATGAGTATGGGGGAGTTATCGTTGATCCAGAACGACTACCAGAAAATGCAGATGCCTTTGCTTACATCCTCCACTCCTCGCTTTCCAATTGGAAAATGGAGGGAAAGAAGGGAATTTGGCTTAAGCTGCCGGTAGAACGAGCTGAGCTTGTTCCAGTTGCTGTTAAGGAAGGTTTCCAATATCACCATGCAGAACGAGGATATGTGATGCTGACATATTGGATTCCAGAAGGACCATGCATGCTTCCCGCTAATGCTTCACATCAAGTAGGGGTTGGGGGATTTGTCATCAATGACAATAATGAGGTTCTTGTCGTACAAGAGAAACACTGTGCTCCTACATGCGTTGGTTTCTGGAAAATACCAACTGGCTTCATCCTTGAGTCGGAAGAGATTTTCACAGGAGCTGTGAGAGAAGTTAAGGAGGAAACTGGGATTGATACGGAGTTTGTGGAAGTTATAGCATTCAGGCATGCTCACAATGTTGCTTTCGAAAAGTCAGATTTGTTCTTTATCTGCATGCTAAAACCACTATCGACTCAGATTATAGTTGATGATCTTGAAATTGAAGCAGCCAAGTGGATGCCCCTGGCTGAGTTTGTGGAGCAACCACTAGTAAAAGAAGATTCCATGTTCAAGAAGGTCATTTCTATATGCATTGCCCGGCTAGGAAAGCGTTACTGCGGTTTATCTTCTCATCAAGTGGTCTCCAAGTTCGATGGCAGGTTGTCTTCCTTGTACTATAACGTCGTTGATACACAAGATTTCAACTGTTCAAATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

378

Amino Acids

42.11

Weight (kDa)

5.46

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nudix_hydro PF18290 114 - 192 8.8e-31 Nudix hydrolase domain
NUDIX PF00293 205 - 325 1.2e-19 NUDIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1065
AciI CCGC 2 cut(s) 600, 1035
AclWI GGATC 1 cut(s) 371
AcsI RAATTY 2 cut(s) 85, 465
AcuI CTGAAG 1 cut(s) 191
AfaI GTAC 5 cut(s) 80, 107, 336, 669, 1091
AfiI CCNNNNNNNGG 2 cut(s) 159, 736
AflII CTTAAG 1 cut(s) 473
AgeI ACCGGT 1 cut(s) 112
AgsI TTSAA 8 cut(s) 32, 204, 281, 914, 920, 988, 1120, 1128
AhlI ACTAGT 1 cut(s) 964
AjnI CCWGG 1 cut(s) 939
AluBI AGCT 6 cut(s) 24, 263, 478, 494, 499, 758
AluI AGCT 6 cut(s) 24, 263, 478, 494, 499, 758
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 2 cut(s) 318, 1063
AlwI GGATC 1 cut(s) 371
AlwNI CAGNNNCTG 2 cut(s) 512, 758
ApeKI GCWGC 3 cut(s) 260, 478, 923
ApoI RAATTY 2 cut(s) 85, 465
ArsI GACNNNNNNTTYG 2 cut(s) 29, 61
AseI ATTAAT 1 cut(s) 11
AsiGI ACCGGT 1 cut(s) 112
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 584
AsuC2I CCSGG 1 cut(s) 1015
AsuHPI GGTGA 1 cut(s) 527
AsuII TTCGAA 1 cut(s) 843
AvaII GGWCC 1 cut(s) 584
BbsI GAAGAC 1 cut(s) 1074
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 3 cut(s) 272, 465, 935
BccI CCATC 1 cut(s) 1064
BciT130I CCWGG 1 cut(s) 941
BcnI CCSGG 1 cut(s) 1015
BcoDI GTCTC 2 cut(s) 318, 1063
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 4 cut(s) 198, 321, 965, 1019
BfrI CTTAAG 1 cut(s) 473
BfuAI ACCTGC 1 cut(s) 1065
BisI GCNGC 3 cut(s) 261, 479, 924
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 3 cut(s) 262, 480, 925
BmcAI AGTACT 1 cut(s) 336
Bme1390I CCNGG 2 cut(s) 941, 1015
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmrFI CCNGG 2 cut(s) 941, 1015
BmrI ACTGGG 1 cut(s) 792
BmsI GCATC 4 cut(s) 331, 394, 549, 924
BmuI ACTGGG 1 cut(s) 792
BpiI GAAGAC 1 cut(s) 1074
Bpu10I CCTNAGC 1 cut(s) 63
Bpu1102I GCTNAGC 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 843
BpuEI CTTGAG 1 cut(s) 752
BpuMI CCSGG 1 cut(s) 1015
BsaI GGTCTC 1 cut(s) 1063
BsaJI CCNNGG 1 cut(s) 939
BsaWI WCCGGW 1 cut(s) 112
BsaXI ACNNNNNCTCC 2 cut(s) 357, 387
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 736
Bse118I RCCGGY 2 cut(s) 112, 481
Bse1I ACTGG 3 cut(s) 506, 724, 787
Bse3DI GCAATG 1 cut(s) 1008
BseBI CCWGG 1 cut(s) 941
BseDI CCNNGG 1 cut(s) 939
BseGI GGATG 5 cut(s) 81, 190, 417, 726, 939
BseLI CCNNNNNNNGG 2 cut(s) 159, 736
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 4 cut(s) 77, 486, 905, 936
BseNI ACTGG 3 cut(s) 506, 724, 787
BseRI GAGGAG 1 cut(s) 418
BseXI GCAGC 3 cut(s) 272, 465, 935
BsgI GTGCAG 1 cut(s) 139
BshTI ACCGGT 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 688
BsiSI CCGG 3 cut(s) 113, 482, 1015
BslI CCNNNNNNNGG 2 cut(s) 159, 736
BsmAI GTCTC 2 cut(s) 318, 1063
BsmI GAATGC 1 cut(s) 815
Bso31I GGTCTC 1 cut(s) 1063
Bsp119I TTCGAA 1 cut(s) 843
Bsp1286I GDGCHC 1 cut(s) 688
Bsp143I GATC 2 cut(s) 376, 907
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 2 cut(s) 600, 1035
BspCNI CTCAG 4 cut(s) 76, 487, 904, 937
BspMI ACCTGC 1 cut(s) 1065
BspPI GGATC 1 cut(s) 371
BspT104I TTCGAA 1 cut(s) 843
BspTI CTTAAG 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 1063
BsrDI GCAATG 1 cut(s) 1008
BsrFI RCCGGY 2 cut(s) 112, 481
BsrI ACTGG 3 cut(s) 506, 724, 787
BssAI RCCGGY 2 cut(s) 112, 481
BssECI CCNNGG 1 cut(s) 939
BssMI GATC 2 cut(s) 376, 907
Bst2UI CCWGG 1 cut(s) 941
Bst4CI ACNGT 3 cut(s) 138, 683, 1124
Bst6I CTCTTC 1 cut(s) 735
BstAFI CTTAAG 1 cut(s) 473
BstBI TTCGAA 1 cut(s) 843
BstC8I GCNNGC 4 cut(s) 154, 593, 825, 872
BstDEI CTNAG 4 cut(s) 63, 495, 891, 945
BstF5I GGATG 5 cut(s) 81, 190, 417, 726, 939
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 2 cut(s) 379, 910
BstMAI GTCTC 2 cut(s) 318, 1063
BstMBI GATC 2 cut(s) 376, 907
BstMWI GCNNNNNNNGC 2 cut(s) 478, 1024
BstNI CCWGG 1 cut(s) 941
BstNSI RCATGY 4 cut(s) 595, 696, 827, 874
BstSCI CCNGG 2 cut(s) 939, 1013
BstV1I GCAGC 3 cut(s) 272, 465, 935
BstV2I GAAGAC 1 cut(s) 1074
BtsCI GGATG 5 cut(s) 81, 190, 417, 726, 939
BtsIMutI CAGTG 1 cut(s) 679
BveI ACCTGC 1 cut(s) 1065
Cac8I GCNNGC 4 cut(s) 154, 593, 825, 872
CaiI CAGNNNCTG 2 cut(s) 512, 758
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 2 cut(s) 112, 481
Cfr13I GGNCC 1 cut(s) 584
Csp6I GTAC 5 cut(s) 79, 106, 335, 668, 1090
CspAI ACCGGT 1 cut(s) 112
CviAII CATG 7 cut(s) 539, 588, 592, 693, 824, 871, 982
CviQI GTAC 5 cut(s) 79, 106, 335, 668, 1090
DdeI CTNAG 4 cut(s) 63, 495, 891, 945
DpnI GATC 2 cut(s) 378, 909
DpnII GATC 2 cut(s) 376, 907
Eam1104I CTCTTC 1 cut(s) 735
EarI CTCTTC 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 1063
Eco32I GATATC 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 1 cut(s) 191
EcoRII CCWGG 1 cut(s) 939
EcoRV GATATC 1 cut(s) 215
EcoT22I ATGCAT 2 cut(s) 593, 1010
FaeI CATG 7 cut(s) 542, 591, 595, 696, 827, 874, 985
FatI CATG 7 cut(s) 538, 587, 591, 692, 823, 870, 981
FauI CCCGC 1 cut(s) 607
Fnu4HI GCNGC 3 cut(s) 261, 479, 924
FokI GGATG 5 cut(s) 88, 177, 404, 713, 946
Fsp4HI GCNGC 3 cut(s) 261, 479, 924
FspBI CTAG 4 cut(s) 198, 321, 965, 1019
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 261, 479, 924
HapII CCGG 3 cut(s) 113, 482, 1015
HhaI GCGC 1 cut(s) 152
Hin1II CATG 7 cut(s) 542, 591, 595, 696, 827, 874, 985
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 5 cut(s) 163, 574, 734, 889, 977
HpaII CCGG 3 cut(s) 113, 482, 1015
HphI GGTGA 1 cut(s) 527
Hpy166II GTNNAC 2 cut(s) 93, 222
Hpy188I TCNGA 5 cut(s) 55, 171, 739, 853, 894
Hpy188III TCNNGA 5 cut(s) 380, 578, 706, 911, 988
Hpy8I GTNNAC 2 cut(s) 93, 222
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 6 cut(s) 166, 204, 454, 515, 575, 985
HpyCH4III ACNGT 3 cut(s) 138, 683, 1124
HpyCH4IV ACGT 1 cut(s) 1098
HpyCH4V TGCA 8 cut(s) 156, 260, 302, 401, 542, 591, 870, 1008
HpyF10VI GCNNNNNNNGC 2 cut(s) 478, 1024
HpyF3I CTNAG 4 cut(s) 63, 495, 891, 945
HpySE526I ACGT 1 cut(s) 1098
Hsp92II CATG 7 cut(s) 542, 591, 595, 696, 827, 874, 985
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 2 cut(s) 376, 907
LmnI GCTCC 3 cut(s) 691, 755, 955
Lsp1109I GCAGC 3 cut(s) 272, 465, 935
LweI GCATC 4 cut(s) 331, 394, 549, 924
MaeI CTAG 4 cut(s) 198, 321, 965, 1019
MaeII ACGT 1 cut(s) 1098
MaeIII GTNAC 1 cut(s) 1028
MalI GATC 2 cut(s) 378, 909
MboI GATC 2 cut(s) 376, 907
MboII GAAGA 5 cut(s) 171, 752, 986, 1035, 1074
MfeI CAATTG 1 cut(s) 439
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 5 cut(s) 85, 439, 465, 915, 1129
MlyI GAGTC 2 cut(s) 743, 883
MmeI TCCRAC 2 cut(s) 223, 717
MnlI CCTC 9 cut(s) 66, 177, 239, 431, 439, 445, 542, 649, 769
Mph1103I ATGCAT 2 cut(s) 593, 1010
MseI TTAA 4 cut(s) 11, 474, 516, 771
MspA1I CMGCKG 1 cut(s) 263
MspCI CTTAAG 1 cut(s) 473
MspI CCGG 3 cut(s) 113, 482, 1015
MspR9I CCNGG 2 cut(s) 941, 1015
MunI CAATTG 1 cut(s) 439
Mva1269I GAATGC 1 cut(s) 815
MvaI CCWGG 1 cut(s) 941
MwoI GCNNNNNNNGC 2 cut(s) 478, 1024
NciI CCSGG 1 cut(s) 1015
NdeII GATC 2 cut(s) 376, 907
NlaIII CATG 7 cut(s) 542, 591, 595, 696, 827, 874, 985
NsiI ATGCAT 2 cut(s) 593, 1010
NspI RCATGY 4 cut(s) 595, 696, 827, 874
NspV TTCGAA 1 cut(s) 843
PaeI GCATGC 3 cut(s) 595, 827, 874
PctI GAATGC 1 cut(s) 815
PfeI GAWTC 3 cut(s) 163, 574, 977
PinAI ACCGGT 1 cut(s) 112
PkrI GCNGC 3 cut(s) 262, 480, 925
PleI GAGTC 2 cut(s) 742, 883
PpsI GAGTC 2 cut(s) 742, 883
PshBI ATTAAT 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 939
PspPI GGNCC 1 cut(s) 584
PstNI CAGNNNCTG 2 cut(s) 512, 758
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 5 cut(s) 80, 107, 336, 669, 1091
RsaNI GTAC 5 cut(s) 79, 106, 335, 668, 1090
SaqAI TTAA 4 cut(s) 11, 474, 516, 771
SatI GCNGC 3 cut(s) 261, 479, 924
Sau3AI GATC 2 cut(s) 376, 907
Sau96I GGNCC 1 cut(s) 584
ScaI AGTACT 1 cut(s) 336
SchI GAGTC 2 cut(s) 743, 883
ScrFI CCNGG 2 cut(s) 941, 1015
SduI GDGCHC 1 cut(s) 688
SfaNI GCATC 4 cut(s) 331, 394, 549, 924
SfuI TTCGAA 1 cut(s) 843
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 2 cut(s) 473, 731
SmoI CTYRAG 2 cut(s) 473, 731
SpeI ACTAGT 1 cut(s) 964
SphI GCATGC 3 cut(s) 595, 827, 874
Sse9I AATT 5 cut(s) 85, 439, 465, 915, 1129
SsiI CCGC 2 cut(s) 600, 1035
SspMI CTAG 4 cut(s) 198, 321, 965, 1019
StyD4I CCNGG 2 cut(s) 939, 1013
TaaI ACNGT 3 cut(s) 138, 683, 1124
TaiI ACGT 1 cut(s) 1101
TaqI TCGA 4 cut(s) 35, 843, 887, 1068
TasI AATT 5 cut(s) 85, 439, 465, 915, 1129
TatI WGTACW 3 cut(s) 78, 334, 1089
TfiI GAWTC 3 cut(s) 163, 574, 977
Tru1I TTAA 4 cut(s) 11, 474, 516, 771
Tru9I TTAA 4 cut(s) 11, 474, 516, 771
TscAI CASTG 1 cut(s) 686
TseI GCWGC 3 cut(s) 260, 478, 923
TspDTI ATGAA 2 cut(s) 171, 715
TspGWI ACGGA 1 cut(s) 809
TspRI CASTG 1 cut(s) 686
Vha464I CTTAAG 1 cut(s) 473
VpaK11BI GGWCC 1 cut(s) 584
VspI ATTAAT 1 cut(s) 11
XapI RAATTY 2 cut(s) 85, 465
XceI RCATGY 4 cut(s) 595, 696, 827, 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 445
XspI CTAG 4 cut(s) 198, 321, 965, 1019
ZrmI AGTACT 1 cut(s) 336
Zsp2I ATGCAT 2 cut(s) 593, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.