Rw6G010050

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000194
Physical Location & Seq
Forward (+)
17294299 .. 17296809
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G010050.1

Sequence Viewer

Length: 2262 bp
ATGCCTGTGCTTATTTCTGAAAACCAGTCGGCGTTTGTCTCTGGTCGCCAAATTCAGGACAATATCTTGGTGGCACATGAGATGTTCCACCATCTCAAACTCTTGAAATCTGGTATTCAGGGGGAATTTGCTCTCAAACTTGACATGAACAAGGCTTATGACCGTGTGGATTGGAATTTTCTGGAGCAGGTGCTACTCAAACTTGGGTTTCACCACACCTGGGTTCGCCTTGTTATGAATTGTGTAACTTCGACTTCCATGACTCTCTTGATCAATGGATCTCCGGGTAGGAAATTTAAACCCACAAGGGGTTTACGACAGGGTGACCCGCTCTCCCCCTTTCTCTTCCTATTTATCAATGATGTGCTATCTACTATGATCAGAAAGATATGTCATGCAGGTCTTTTAGATGCTATCAAGCTAAACACTGATGGTCCTTTGGTAAGCCACCTCTTTTTTGCTGATGACTCAATCTTCTTTCTCAAGGCCACTTTGTCAAATTGCGAGGTGTTGTCTGATACAATTCATCACTACTGCTGTGCCTCGGGTCAGTCTATCAATAAGCAAAAGTCCACTCTCTTTTTCAGCCCCAACACCCAACCACAGATTGCTCATCTCATTAGCTTGGTTCTAGATATGAAATCGGTCCCAAACCCGGGCCGATACTTAGGTCTCCCTACAGTTTGGGGCAGATCAAAGATCTCAGCTTTGGCTTATGTTAAAGAAAACATACTTCAGAAGATTTCGGGTTGGTCCAAGTCAACTCTATCTCAAGCAGGCAAGGAGATATTGATCAAATCCGTTGCAATGGCAGTCCCGATGTACCCAATGACTGTTTTCAAGTTCCCGGCAGGGCTCTCAAATCAAATAAACTCGGTGTTGGCTGATTTCTGGTGGGGGAATGCTTCATCCCCGGGTATGCACTGGAAAAAATGGGCTTCTCTTGGTATTCCAAAATCAAATGGAGGTTTGGGTTTCCGGAATTTGGCTGATTTTAACATTGCTTTGCTTGCAAAGCAGGGTTGGCGCATCCTTTCCAATCCGAATGCATTGTGGGTAAGAGTTCTAAAAAGTAGATATTTTCCTCGTTCCTCTTTCCTAGAGGCCACCCGTGGTTCTTCCCCTTCATGGGCTTGGTCTAGTCTCCTTGAAGGTAGGCACCTCATCATGGGTGGAGGAATGTGGCAGGTTGGTTCTGGATCCCAAATTGATTTCTGGAAGGATCACTGGATCCCTAGATGCCCCTCTGGTAACCTCCTCCAATACAGGCCCCAAAATGGCCCTTTTCTGAACGCGATGAATGTCTCCCATATCATCGATAGATCATCCAACTCTTGGAACCTGGATCTGGTATCATCTTTCATTCCTTTGCAGATCTTGAATGCCATTCAAGCTACCACGTTTGGCTCCCAGAACGACCATGACAAGCTAATTTGGGCTCCCACTTCTTCAGGGGTTTATTCGGTCAAGTCAGGTTATCCACTTCAAGTTTCTGGTTCTTCACATAGCCTTGCCTCTCACCCTCATCAATCCCATTCTATTGACTCCAAAGTTTGGATTTGGCTATGGAAAGTCCGAGCTACTCCGAGAGTGAAACATTTCCTTTGGAGAGCTCTGTCAAATGTCTTGGCTACAAATCACAATCGTCACACTAGACACTTGACTCAATCTTCCTTGTGTCCGCTGTGTCAAGCTCACCCTGAAACGGTTGAACACATTCTCTTGTTATGCCCATGGGCCATGCGTACCTGGTTTGCCCACCCACTCTCCTATAGAATTGACCCTCAGACCATCACTTCTTTGGACAGGTGGCTTACAGAGTTGTTCCTTTCAAATGGTGGCCTTTATGCGGCTTGGGATTCTTCCACTCTTTCTTGTGGGCTCTCAGCTCTATTACGTGACTCTACTGGAACTTTAGTAAAAGGCTTGTCTCAGTCGAATTTTGCTTCTTCCTCCTTGGCTGCTGAAGCTATGGCAATTGATCTTGGCCTCAGCCTTGCCTCCTCTATACCTCTATCTTCTTTTCAGTTGGAATCGGACTCTTTGGTTTTGATATCAGCTTTTTTAAACCCTTTGACTGCGGTTGACTGGTCGGCTTCTCAGATTGTTTCCACCATCCGAACGAAGACTTCTAGCTTCAACTGTGTAAACTGGCGTTGGACCAGTAGGAGGACAAACGCTGCTGCGGATCTTGTGGCAGCGTGGGCCTCGCGCAGGGTGTGCCATATGGATTGGGACTCTAATCCCCCCCCCCCTCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

753

Amino Acids

83.85

Weight (kDa)

9.55

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 4 - 225 4.3e-21 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 512 - 584 4.3e-16 zinc-binding in reverse transcriptase
RVT_3 PF13456 618 - 736 1.6e-10 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 178
Acc36I ACCTGC 3 cut(s) 178, 389, 1177
AccB1I GGYRCC 1 cut(s) 1158
AccB7I CCANNNNNTGG 2 cut(s) 1335, 1554
AccBSI CCGCTC 1 cut(s) 331
AccII CGCG 2 cut(s) 1295, 2212
AccIII TCCGGA 1 cut(s) 978
AciI CCGC 5 cut(s) 329, 1682, 1850, 2081, 2186
AclWI GGATC 8 cut(s) 286, 1194, 1207, 1225, 1230, 1238, 1353, 2196
AcsI RAATTY 6 cut(s) 51, 125, 175, 293, 982, 1939
AcuI CTGAAG 3 cut(s) 719, 1434, 1986
AfaI GTAC 2 cut(s) 824, 1747
AgsI TTSAA 9 cut(s) 106, 841, 1151, 1381, 1391, 1487, 1712, 1833, 2140
AjnI CCWGG 3 cut(s) 218, 1341, 1748
Alw21I GWGCWC 1 cut(s) 1615
Alw26I GTCTC 5 cut(s) 43, 677, 1148, 1309, 1935
AlwI GGATC 8 cut(s) 286, 1194, 1207, 1225, 1230, 1238, 1353, 2196
Ama87I CYCGRG 3 cut(s) 544, 655, 913
Aor13HI TCCGGA 1 cut(s) 978
AoxI GGCC 9 cut(s) 486, 658, 1104, 1268, 1279, 1737, 1840, 1987, 2205
ApeKI GCWGC 4 cut(s) 1961, 2180, 2183, 2198
ApoI RAATTY 6 cut(s) 51, 125, 175, 293, 982, 1939
Asp700I GAANNNNTTC 2 cut(s) 1598, 1716
AspLEI GCGC 2 cut(s) 1029, 2214
AspS9I GGNCC 9 cut(s) 434, 646, 658, 753, 1269, 1280, 1737, 2160, 2205
AsuC2I CCSGG 6 cut(s) 285, 656, 657, 848, 914, 915
AsuHPI GGTGA 4 cut(s) 203, 335, 1511, 1688
AvaI CYCGRG 3 cut(s) 544, 655, 913
AvaII GGWCC 4 cut(s) 434, 646, 753, 2160
BaeI ACNNNNGTAYC 2 cut(s) 655, 688
BamHI GGATCC 2 cut(s) 1199, 1230
BanI GGYRCC 1 cut(s) 1158
BanII GRGCYC 4 cut(s) 858, 1441, 1615, 1884
BbsI GAAGAC 1 cut(s) 2132
Bbv12I GWGCWC 1 cut(s) 1615
BbvCI CCTCAGC 1 cut(s) 1991
BbvI GCAGC 4 cut(s) 1948, 2167, 2170, 2210
BccI CCATC 4 cut(s) 99, 425, 1799, 2123
BciT130I CCWGG 3 cut(s) 220, 1343, 1750
BclI TGATCA 3 cut(s) 270, 378, 792
BcnI CCSGG 6 cut(s) 285, 656, 657, 848, 914, 915
BcoDI GTCTC 5 cut(s) 43, 677, 1148, 1309, 1935
BfaI CTAG 6 cut(s) 632, 1100, 1140, 1236, 1653, 2133
BfmI CTRYAG 2 cut(s) 678, 1771
BfuAI ACCTGC 3 cut(s) 178, 389, 1177
BglII AGATCT 2 cut(s) 699, 1374
BisI GCNGC 5 cut(s) 1851, 1962, 2181, 2184, 2199
BlsI GCNGC 5 cut(s) 1852, 1963, 2182, 2185, 2200
Bme1390I CCNGG 9 cut(s) 220, 285, 656, 657, 848, 914, 915, 1343, 1750
Bme18I GGWCC 4 cut(s) 434, 646, 753, 2160
BmeT110I CYCGRG 3 cut(s) 544, 655, 913
BmgT120I GGNCC 9 cut(s) 434, 646, 658, 753, 1269, 1280, 1737, 2160, 2205
BmiI GGNNCC 8 cut(s) 648, 1160, 1201, 1232, 1271, 1340, 1408, 1440
BmrFI CCNGG 9 cut(s) 220, 285, 656, 657, 848, 914, 915, 1343, 1750
BmsI GCATC 3 cut(s) 400, 1038, 1229
BpiI GAAGAC 1 cut(s) 2132
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 1991
BpuEI CTTGAG 2 cut(s) 467, 756
BpuMI CCSGG 6 cut(s) 285, 656, 657, 848, 914, 915
Bsa29I ATCGAT 1 cut(s) 1317
BsaAI YACGTR 1 cut(s) 1898
BsaBI GATNNNNATC 1 cut(s) 791
BsaI GGTCTC 1 cut(s) 677
BsaJI CCNNGG 8 cut(s) 219, 543, 655, 912, 913, 1111, 1733, 1956
BsaWI WCCGGW 1 cut(s) 978
BsaXI ACNNNNNCTCC 4 cut(s) 317, 347, 1751, 1781
Bse1I ACTGG 7 cut(s) 25, 929, 1232, 1912, 2093, 2156, 2163
Bse3DI GCAATG 2 cut(s) 813, 999
Bse8I GATNNNNATC 1 cut(s) 791
BseAI TCCGGA 1 cut(s) 978
BseBI CCWGG 3 cut(s) 220, 1343, 1750
BseCI ATCGAT 1 cut(s) 1317
BseDI CCNNGG 8 cut(s) 219, 543, 655, 912, 913, 1111, 1733, 1956
BseGI GGATG 4 cut(s) 908, 1029, 1325, 2115
BseJI GATNNNNATC 1 cut(s) 791
BseMI GCAATG 2 cut(s) 813, 999
BseMII CTCAG 6 cut(s) 717, 1799, 1899, 1946, 2005, 2114
BseNI ACTGG 7 cut(s) 25, 929, 1232, 1912, 2093, 2156, 2163
BseRI GAGGAG 2 cut(s) 1247, 1993
BseXI GCAGC 4 cut(s) 1948, 2167, 2170, 2210
Bsh1236I CGCG 2 cut(s) 1295, 2212
BshFI GGCC 9 cut(s) 488, 660, 1106, 1270, 1281, 1739, 1842, 1989, 2207
BshNI GGYRCC 1 cut(s) 1158
BshVI ATCGAT 1 cut(s) 1317
BsiHKAI GWGCWC 1 cut(s) 1615
BsiHKCI CYCGRG 3 cut(s) 544, 655, 913
BsiSI CCGG 5 cut(s) 284, 656, 848, 914, 979
BslFI GGGAC 3 cut(s) 632, 800, 2249
BsmAI GTCTC 5 cut(s) 43, 677, 1148, 1309, 1935
BsmFI GGGAC 3 cut(s) 632, 800, 2249
BsmI GAATGC 3 cut(s) 907, 1051, 1387
BsnI GGCC 9 cut(s) 488, 660, 1106, 1270, 1281, 1739, 1842, 1989, 2207
Bso31I GGTCTC 1 cut(s) 677
BsoBI CYCGRG 3 cut(s) 544, 655, 913
Bsp1286I GDGCHC 4 cut(s) 858, 1441, 1615, 1884
Bsp13I TCCGGA 1 cut(s) 978
Bsp19I CCATGG 1 cut(s) 1733
BspACI CCGC 5 cut(s) 329, 1682, 1850, 2081, 2186
BspANI GGCC 9 cut(s) 488, 660, 1106, 1270, 1281, 1739, 1842, 1989, 2207
BspCNI CTCAG 6 cut(s) 716, 1798, 1898, 1945, 2004, 2113
BspDI ATCGAT 1 cut(s) 1317
BspEI TCCGGA 1 cut(s) 978
BspFNI CGCG 2 cut(s) 1295, 2212
BspLI GGNNCC 8 cut(s) 648, 1160, 1201, 1232, 1271, 1340, 1408, 1440
BspMI ACCTGC 3 cut(s) 178, 389, 1177
BspPI GGATC 8 cut(s) 286, 1194, 1207, 1225, 1230, 1238, 1353, 2196
BspT107I GGYRCC 1 cut(s) 1158
BspTNI GGTCTC 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 331
BsrDI GCAATG 2 cut(s) 813, 999
BsrI ACTGG 7 cut(s) 25, 929, 1232, 1912, 2093, 2156, 2163
BssECI CCNNGG 8 cut(s) 219, 543, 655, 912, 913, 1111, 1733, 1956
BssT1I CCWWGG 2 cut(s) 1733, 1956
Bst2UI CCWGG 3 cut(s) 220, 1343, 1750
Bst4CI ACNGT 5 cut(s) 164, 682, 835, 1708, 2144
Bst6I CTCTTC 1 cut(s) 350
BstAPI GCANNNNNTGC 1 cut(s) 2220
BstBAI YACGTR 1 cut(s) 1898
BstC8I GCNNGC 2 cut(s) 778, 1011
BstDEI CTNAG 7 cut(s) 667, 703, 1785, 1885, 1932, 1991, 2100
BstDSI CCRYGG 2 cut(s) 1111, 1733
BstEII GGTNACC 2 cut(s) 323, 1250
BstENI CCTNNNNNAGG 1 cut(s) 2212
BstF5I GGATG 4 cut(s) 908, 1029, 1325, 2115
BstFNI CGCG 2 cut(s) 1295, 2212
BstHHI GCGC 2 cut(s) 1029, 2214
BstMAI GTCTC 5 cut(s) 43, 677, 1148, 1309, 1935
BstMWI GCNNNNNNNGC 7 cut(s) 1010, 1015, 1024, 1391, 1967, 2204, 2220
BstNI CCWGG 3 cut(s) 220, 1343, 1750
BstPI GGTNACC 2 cut(s) 323, 1250
BstSCI CCNGG 9 cut(s) 218, 283, 654, 655, 846, 912, 913, 1341, 1748
BstSFI CTRYAG 2 cut(s) 678, 1771
BstUI CGCG 2 cut(s) 1295, 2212
BstV1I GCAGC 4 cut(s) 1948, 2167, 2170, 2210
BstV2I GAAGAC 1 cut(s) 2132
BstX2I RGATCY 7 cut(s) 278, 699, 1199, 1230, 1345, 1374, 2188
BstYI RGATCY 7 cut(s) 278, 699, 1199, 1230, 1345, 1374, 2188
Bsu15I ATCGAT 1 cut(s) 1317
BsuRI GGCC 9 cut(s) 488, 660, 1106, 1270, 1281, 1739, 1842, 1989, 2207
BsuTUI ATCGAT 1 cut(s) 1317
BtgI CCRYGG 2 cut(s) 1111, 1733
BtgZI GCGATG 1 cut(s) 1310
BtsCI GGATG 4 cut(s) 908, 1029, 1325, 2115
BtsIMutI CAGTG 3 cut(s) 426, 922, 1225
BveI ACCTGC 3 cut(s) 178, 389, 1177
Cac8I GCNNGC 2 cut(s) 778, 1011
CfoI GCGC 2 cut(s) 1029, 2214
Cfr13I GGNCC 9 cut(s) 434, 646, 658, 753, 1269, 1280, 1737, 2160, 2205
Cfr9I CCCGGG 2 cut(s) 655, 913
ClaI ATCGAT 1 cut(s) 1317
CsiI ACCWGGT 1 cut(s) 1748
Csp6I GTAC 2 cut(s) 823, 1746
CviAII CATG 9 cut(s) 77, 145, 259, 395, 1128, 1168, 1421, 1734, 1741
CviQI GTAC 2 cut(s) 823, 1746
DdeI CTNAG 7 cut(s) 667, 703, 1785, 1885, 1932, 1991, 2100
DraI TTTAAA 2 cut(s) 298, 2067
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 1613
Eco130I CCWWGG 2 cut(s) 1733, 1956
Eco24I GRGCYC 4 cut(s) 858, 1441, 1615, 1884
Eco31I GGTCTC 1 cut(s) 677
Eco32I GATATC 1 cut(s) 2055
Eco47I GGWCC 4 cut(s) 434, 646, 753, 2160
Eco53kI GAGCTC 1 cut(s) 1613
Eco57I CTGAAG 3 cut(s) 719, 1434, 1986
Eco88I CYCGRG 3 cut(s) 544, 655, 913
Eco91I GGTNACC 2 cut(s) 323, 1250
EcoICRI GAGCTC 1 cut(s) 1613
EcoNI CCTNNNNNAGG 1 cut(s) 2212
EcoO109I RGGNCCY 1 cut(s) 1269
EcoO65I GGTNACC 2 cut(s) 323, 1250
EcoRII CCWGG 3 cut(s) 218, 1341, 1748
EcoRV GATATC 1 cut(s) 2055
EcoT14I CCWWGG 2 cut(s) 1733, 1956
EcoT22I ATGCAT 1 cut(s) 1051
EcoT38I GRGCYC 4 cut(s) 858, 1441, 1615, 1884
ErhI CCWWGG 2 cut(s) 1733, 1956
FaeI CATG 9 cut(s) 80, 148, 262, 398, 1131, 1171, 1424, 1737, 1744
FaqI GGGAC 3 cut(s) 632, 800, 2249
FatI CATG 9 cut(s) 76, 144, 258, 394, 1127, 1167, 1420, 1733, 1740
FauI CCCGC 1 cut(s) 336
FauNDI CATATG 1 cut(s) 2226
FbaI TGATCA 3 cut(s) 270, 378, 792
Fnu4HI GCNGC 5 cut(s) 1851, 1962, 2181, 2184, 2199
FokI GGATG 4 cut(s) 895, 1016, 1312, 2102
FriOI GRGCYC 4 cut(s) 858, 1441, 1615, 1884
Fsp4HI GCNGC 5 cut(s) 1851, 1962, 2181, 2184, 2199
FspBI CTAG 6 cut(s) 632, 1100, 1140, 1236, 1653, 2133
GlaI GCGC 2 cut(s) 1028, 2213
GluI GCNGC 5 cut(s) 1851, 1962, 2181, 2184, 2199
GsuI CTGGAG 1 cut(s) 203
HaeIII GGCC 9 cut(s) 488, 660, 1106, 1270, 1281, 1739, 1842, 1989, 2207
HapII CCGG 5 cut(s) 284, 656, 848, 914, 979
HhaI GCGC 2 cut(s) 1029, 2214
Hin1II CATG 9 cut(s) 80, 148, 262, 398, 1131, 1171, 1424, 1737, 1744
Hin6I GCGC 2 cut(s) 1027, 2212
HinP1I GCGC 2 cut(s) 1027, 2212
HincII GTYRAC 2 cut(s) 762, 2086
HindII GTYRAC 2 cut(s) 762, 2086
HinfI GANTC 9 cut(s) 262, 467, 1544, 1663, 1859, 1901, 2033, 2039, 2237
HpaII CCGG 5 cut(s) 284, 656, 848, 914, 979
HphI GGTGA 4 cut(s) 203, 335, 1511, 1688
Hpy166II GTNNAC 5 cut(s) 314, 573, 762, 2086, 2149
Hpy8I GTNNAC 5 cut(s) 314, 573, 762, 2086, 2149
HpyAV CCTTC 3 cut(s) 1134, 1145, 1213
HpyCH4III ACNGT 5 cut(s) 164, 682, 835, 1708, 2144
HpyCH4IV ACGT 2 cut(s) 1400, 1897
HpyCH4V TGCA 6 cut(s) 398, 806, 922, 1013, 1049, 1372
HpyF10VI GCNNNNNNNGC 7 cut(s) 1010, 1015, 1024, 1391, 1967, 2204, 2220
HpyF3I CTNAG 7 cut(s) 667, 703, 1785, 1885, 1932, 1991, 2100
HpySE526I ACGT 2 cut(s) 1400, 1897
Hsp92II CATG 9 cut(s) 80, 148, 262, 398, 1131, 1171, 1424, 1737, 1744
HspAI GCGC 2 cut(s) 1027, 2212
Kpn2I TCCGGA 1 cut(s) 978
Ksp22I TGATCA 3 cut(s) 270, 378, 792
LmnI GCTCC 3 cut(s) 184, 1412, 1444
Lsp1109I GCAGC 4 cut(s) 1948, 2167, 2170, 2210
LweI GCATC 3 cut(s) 400, 1038, 1229
MabI ACCWGGT 1 cut(s) 1748
MaeI CTAG 6 cut(s) 632, 1100, 1140, 1236, 1653, 2133
MaeII ACGT 2 cut(s) 1400, 1897
MaeIII GTNAC 5 cut(s) 244, 323, 1250, 1646, 1898
MbiI CCGCTC 1 cut(s) 331
MfeI CAATTG 1 cut(s) 1977
MflI RGATCY 7 cut(s) 278, 699, 1199, 1230, 1345, 1374, 2188
MhlI GDGCHC 4 cut(s) 858, 1441, 1615, 1884
MlyI GAGTC 7 cut(s) 256, 461, 1538, 1657, 1895, 2033, 2231
MmeI TCCRAC 3 cut(s) 1353, 2010, 2138
Mph1103I ATGCAT 1 cut(s) 1051
MroI TCCGGA 1 cut(s) 978
MroXI GAANNNNTTC 2 cut(s) 1598, 1716
MseI TTAA 4 cut(s) 297, 720, 996, 2066
MspA1I CMGCKG 1 cut(s) 1684
MspI CCGG 5 cut(s) 284, 656, 848, 914, 979
MspR9I CCNGG 9 cut(s) 220, 285, 656, 657, 848, 914, 915, 1343, 1750
MunI CAATTG 1 cut(s) 1977
Mva1269I GAATGC 3 cut(s) 907, 1051, 1387
MvaI CCWGG 3 cut(s) 220, 1343, 1750
MvnI CGCG 2 cut(s) 1295, 2212
MwoI GCNNNNNNNGC 7 cut(s) 1010, 1015, 1024, 1391, 1967, 2204, 2220
NciI CCSGG 6 cut(s) 285, 656, 657, 848, 914, 915
NcoI CCATGG 1 cut(s) 1733
NdeI CATATG 1 cut(s) 2226
NlaIII CATG 9 cut(s) 80, 148, 262, 398, 1131, 1171, 1424, 1737, 1744
NlaIV GGNNCC 8 cut(s) 648, 1160, 1201, 1232, 1271, 1340, 1408, 1440
NmuCI GTSAC 3 cut(s) 323, 1646, 1898
NsiI ATGCAT 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 178
PctI GAATGC 3 cut(s) 907, 1051, 1387
PdmI GAANNNNTTC 2 cut(s) 1598, 1716
PfeI GAWTC 2 cut(s) 1859, 2033
PflMI CCANNNNNTGG 2 cut(s) 1335, 1554
PkrI GCNGC 5 cut(s) 1852, 1963, 2182, 2185, 2200
PleI GAGTC 7 cut(s) 256, 461, 1538, 1657, 1895, 2033, 2231
PpsI GAGTC 7 cut(s) 256, 461, 1538, 1657, 1895, 2033, 2231
Ppu21I YACGTR 1 cut(s) 1898
Psp124BI GAGCTC 1 cut(s) 1615
Psp6I CCWGG 3 cut(s) 218, 1341, 1748
PspEI GGTNACC 2 cut(s) 323, 1250
PspGI CCWGG 3 cut(s) 218, 1341, 1748
PspN4I GGNNCC 8 cut(s) 648, 1160, 1201, 1232, 1271, 1340, 1408, 1440
PspPI GGNCC 9 cut(s) 434, 646, 658, 753, 1269, 1280, 1737, 2160, 2205
PsuI RGATCY 7 cut(s) 278, 699, 1199, 1230, 1345, 1374, 2188
RsaI GTAC 2 cut(s) 824, 1747
RsaNI GTAC 2 cut(s) 823, 1746
SacI GAGCTC 1 cut(s) 1615
SaqAI TTAA 4 cut(s) 297, 720, 996, 2066
SatI GCNGC 5 cut(s) 1851, 1962, 2181, 2184, 2199
Sau96I GGNCC 9 cut(s) 434, 646, 658, 753, 1269, 1280, 1737, 2160, 2205
SchI GAGTC 7 cut(s) 256, 461, 1538, 1657, 1895, 2033, 2231
ScrFI CCNGG 9 cut(s) 220, 285, 656, 657, 848, 914, 915, 1343, 1750
SduI GDGCHC 4 cut(s) 858, 1441, 1615, 1884
SexAI ACCWGGT 1 cut(s) 1748
SfaNI GCATC 3 cut(s) 400, 1038, 1229
SfcI CTRYAG 2 cut(s) 678, 1771
SinI GGWCC 4 cut(s) 434, 646, 753, 2160
SmaI CCCGGG 2 cut(s) 657, 915
SmlI CTYRAG 2 cut(s) 482, 771
SmoI CTYRAG 2 cut(s) 482, 771
SsiI CCGC 5 cut(s) 329, 1682, 1850, 2081, 2186
SspMI CTAG 6 cut(s) 632, 1100, 1140, 1236, 1653, 2133
SstI GAGCTC 1 cut(s) 1615
StyD4I CCNGG 9 cut(s) 218, 283, 654, 655, 846, 912, 913, 1341, 1748
StyI CCWWGG 2 cut(s) 1733, 1956
TaaI ACNGT 5 cut(s) 164, 682, 835, 1708, 2144
TaiI ACGT 2 cut(s) 1403, 1900
TaqI TCGA 3 cut(s) 251, 1317, 1937
TaqII GACCGA 2 cut(s) 634, 1453
TauI GCSGC 1 cut(s) 1853
TfiI GAWTC 2 cut(s) 1859, 2033
Tru1I TTAA 4 cut(s) 297, 720, 996, 2066
Tru9I TTAA 4 cut(s) 297, 720, 996, 2066
TscAI CASTG 3 cut(s) 433, 929, 1232
TseFI GTSAC 3 cut(s) 323, 1646, 1898
TseI GCWGC 4 cut(s) 1961, 2180, 2183, 2198
Tsp45I GTSAC 3 cut(s) 323, 1646, 1898
TspDTI ATGAA 8 cut(s) 161, 251, 515, 653, 897, 1116, 1313, 1351
TspGWI ACGGA 1 cut(s) 790
TspMI CCCGGG 2 cut(s) 655, 913
TspRI CASTG 3 cut(s) 433, 929, 1232
Van91I CCANNNNNTGG 2 cut(s) 1335, 1554
VpaK11BI GGWCC 4 cut(s) 434, 646, 753, 2160
XagI CCTNNNNNAGG 1 cut(s) 2212
XapI RAATTY 6 cut(s) 51, 125, 175, 293, 982, 1939
XbaI TCTAGA 1 cut(s) 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 1798
XmaI CCCGGG 2 cut(s) 655, 913
XmnI GAANNNNTTC 2 cut(s) 1598, 1716
XspI CTAG 6 cut(s) 632, 1100, 1140, 1236, 1653, 2133
Zsp2I ATGCAT 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.