Rw1G021820

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000194
Physical Location & Seq
Reverse (-)
46262507 .. 46265108
2602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021820.1

Sequence Viewer

Length: 2316 bp
ATGCCATCCCTAATTTCTGAGAATCAGTCCGCTTTTGTCTCTGGTAGACAGATTCAGGACAACATTTTGGTGGCCCATGAATTGTTTCACCACCTCAAGTTACTCAAGTCAGGTTCAGTCGGGGAATTTGCAATTAAACTGGACATGAACAAGGCCTATGATCGTGTGGACTGGAAGTTTTTGGAGCTGGTTCTTCTGAAGATGGGATTCCACCAAAATTGGGTGAGATTGGTAATGTCTTGTGTTACCTCTGTCTCAATGGCTATTATTGTCAATGGAGCTCCTGGTCGCAAATTCAAACCAACCCGTGGTCTCAGGCAGGGAGATCCTCTCTCCCCTTTCCTCTTCCTTTTTATCAATGATGTTTTATCCACCATGATCCGGAAAATATGTGACGCTAACTTACTGGATGCTGTTAAATTAAGCCCGAATGGTCCATTGGTTAGTCATCTTTTCTTTGCAGATGATTCCATCTTTTTTCTCAAAGCTACACTGTACAATTGCGAAGTACTTTCCGATACTCTGCATCACTATTGTAGTGCCTCTGGTCAATCCATTAATCTCCACAAATCTTCTCTCTTCTTTAGTCCCAACACCCCTCCAGAAATTGTTAACCTCATCAGCATGGTGTTGGGTATGCAACCAGTTTTGAATCTGGGGTTTTATCTTGGTCTGCCGACCATCTGGGGCCGATCAAAAGTTTCGGCTTTGGCTTATGTTAAAGAGCACATCGAAAAGAAAATTTCGGGGTGGAATACTTCCACTTTATCCCAAGCTGGAAAAGAAATCATGATCAAATCTGTGGCAATGGCAATCCCTGCCTACCCAATGTCGGTCTTCAAATTCCCTTCTTCTCTTTGCAACCAAATCAACTCTGCGCTTGCAAATTTCTGGTGGGGAAATGACACCACTACGGGTATACACTGGAAAAATTGGTCATCTCTGGGCTTGCCTAAATCTTGTGGAGGTATGGGTTTTCGTTGTATTGAATCTTTCAATTATGCCCTTCTTGCCAAACAGGGTTGGAGAATCCTTGCCAATCCCAATGCACTCTGGGTCCGTGTACTGAAGAGTCGTTACTTCCCCTCCTGCACCTTCTTTGAAGCAACTCGGGGATCCTCACCATCTTGGGCTTGGGCCAGCATTTTGGATGCCAGGCATATTCTCTCGGATGGTTGTATTTGGCAGGTTGGATCTGGTCTTTCTATTGATTTCTGGAGAGACAATTGGATCCCCCGACTACCGTCTTGCAATTTGAAAGATTTTATGCCTCTCAACACCCCGGAAACCCCGGTCAGTTTGTTTGTCGACTCTCTGAATCACACATGGAATCTGGATGCGGTCTCTCCTTTCCTCTCAGTGGATGCTATCAGAGCTATTAAAGCTACCCCTTTCGGAAATCCGGAAGATGAAGACTCTATTCTTTGGCCGGCAACCTCAACGGGCATTTACTCCGTTAGGACGGGTTATGAAGCAAAAATCAAAGACTCCATGCCCTCACTTGTTGTTCATCCTCACAGGTCGCACTCAATCCCCTCAAGCACTTGGAAATGGCTCTGGAATCTTAAGGTGACTCCAAGAATCAAACACTTCCTATGGCGAGCTCTTTCTGGTGCTTTAGCAACAAACCACAACAGACACCAAAGGCACCTTGCATCTTCCCCACTCTGCACCTTCTGTGAGGCTCATCCTGAAACAATTGAACATGTTCTATTATTATGCCCTTGGGCAAGAAGAACGTGGTTCTGCCATCCCCTCTCTTACAGGATTGACCCCCAAGCCGTGACTTCTCTTGATATTTGGATTTCCGCGATCTTCTCCCCGGATTCGTCATCATCTCTACCTGATCAGATTAACACTGATGCGGCTTGGAATGCGGATTCTTTATCTTGTGGTCTGGGTACCCTGATTCGTGATTCTAAGGGTGCTGTTCTTGGGGGGTTAGCCAAATCATCACATGCGATGTCCTCTCTGGCAGCAGAAGCCATGGCTATTGTCCTTGGTCTCTCTCTTGCCTCATCCCTCTCCCTTTCTTCTTTTCAGTTGGAATCAGACTCCTTGGTTTTGATTTCACATTTGTTAAATCCTCTTTCTTCGATTGATTGGTCTGCAACTCAAATCGTCTCTCGGATTCGAGCTTTGGCTGGCCACTTCGATCGTGTTAACTGGCTTTGGACCAGTAGACATGCTAATTGTGCTGCGGACTCTATTGCGGCTTTGGCATCTCGCAGGGTGTGCACGGATGACTGGGTGTCCAATCCTCCCCCCTCCCTAATGCGAATTCTTCTCTTTGATGCAGCAGGTCCTCCCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

771

Amino Acids

85.5

Weight (kDa)

8.72

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 4 - 221 5.7e-20 Reverse transcriptase (RNA-dependent DNA polymerase)
zf-RVT PF13966 508 - 581 3.9e-17 zinc-binding in reverse transcriptase
RVT_3 PF13456 619 - 740 7.7e-19 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1177, 2291
Acc65I GGTACC 1 cut(s) 1901
AccB1I GGYRCC 2 cut(s) 1647, 1901
AccB7I CCANNNNNTGG 1 cut(s) 308
AccI GTMKAC 4 cut(s) 46, 919, 1308, 2182
AccII CGCG 1 cut(s) 1811
AccIII TCCGGA 2 cut(s) 381, 1402
AciI CCGC 7 cut(s) 30, 1340, 1809, 1865, 1877, 2201, 2213
AclWI GGATC 7 cut(s) 320, 373, 1110, 1123, 1201, 1225, 1238
AcoI YGGCCR 2 cut(s) 1427, 2146
AcsI RAATTY 6 cut(s) 125, 293, 741, 842, 886, 2280
AcuI CTGAAG 2 cut(s) 218, 1088
AfaI GTAC 4 cut(s) 497, 510, 1065, 1903
AfiI CCNNNNNNNGG 5 cut(s) 220, 308, 381, 832, 1360
AflII CTTAAG 1 cut(s) 1565
AflIII ACRYGT 1 cut(s) 1705
AgsI TTSAA 8 cut(s) 298, 652, 841, 989, 997, 1103, 1258, 1703
AhdI GACNNNNNGTC 1 cut(s) 2251
AjnI CCWGG 2 cut(s) 283, 1154
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AluBI AGCT 8 cut(s) 187, 281, 488, 776, 1376, 1385, 1604, 2138
AluI AGCT 8 cut(s) 187, 281, 488, 776, 1376, 1385, 1604, 2138
Alw21I GWGCWC 4 cut(s) 283, 729, 1606, 2240
Alw26I GTCTC 7 cut(s) 43, 259, 317, 1215, 1348, 2009, 2128
Alw44I GTGCAC 1 cut(s) 2236
AlwI GGATC 7 cut(s) 320, 373, 1110, 1123, 1201, 1225, 1238
Ama87I CYCGRG 1 cut(s) 1110
Aor13HI TCCGGA 2 cut(s) 381, 1402
AoxI GGCC 6 cut(s) 72, 153, 688, 1137, 1427, 2146
ApaLI GTGCAC 1 cut(s) 2236
ApeKI GCWGC 3 cut(s) 1976, 2198, 2297
ApoI RAATTY 6 cut(s) 125, 293, 741, 842, 886, 2280
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 2 cut(s) 84, 1707
Asp718I GGTACC 1 cut(s) 1901
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 7 cut(s) 73, 434, 688, 1057, 1137, 2175, 2303
AsuC2I CCSGG 3 cut(s) 1283, 1292, 1823
AsuHPI GGTGA 4 cut(s) 80, 235, 1113, 1582
AvaI CYCGRG 1 cut(s) 1110
AvaII GGWCC 4 cut(s) 434, 1057, 2175, 2303
BaeGI GKGCMC 1 cut(s) 2240
BalI TGGCCA 1 cut(s) 2148
BamHI GGATCC 2 cut(s) 1115, 1230
BanI GGYRCC 2 cut(s) 1647, 1901
BanII GRGCYC 2 cut(s) 283, 1606
BarI GAAGNNNNNNTAC 2 cut(s) 1061, 1093
BbsI GAAGAC 2 cut(s) 829, 1419
Bbv12I GWGCWC 4 cut(s) 283, 729, 1606, 2240
BbvI GCAGC 3 cut(s) 1988, 2185, 2309
BccI CCATC 7 cut(s) 13, 196, 479, 689, 1132, 1166, 1758
BceAI ACGGC 1 cut(s) 1766
BciT130I CCWGG 2 cut(s) 285, 1156
BclI TGATCA 2 cut(s) 792, 1846
BcnI CCSGG 3 cut(s) 1283, 1292, 1823
BcoDI GTCTC 7 cut(s) 43, 259, 317, 1215, 1348, 2009, 2128
BfrI CTTAAG 1 cut(s) 1565
BfuAI ACCTGC 2 cut(s) 1177, 2291
BisI GCNGC 5 cut(s) 1866, 1977, 2199, 2214, 2298
BlsI GCNGC 5 cut(s) 1867, 1978, 2200, 2215, 2299
BmcAI AGTACT 1 cut(s) 510
Bme1390I CCNGG 5 cut(s) 285, 1156, 1283, 1292, 1823
Bme18I GGWCC 4 cut(s) 434, 1057, 2175, 2303
BmeRI GACNNNNNGTC 1 cut(s) 2251
BmeT110I CYCGRG 1 cut(s) 1110
BmgT120I GGNCC 7 cut(s) 73, 434, 688, 1057, 1137, 2175, 2303
BmiI GGNNCC 6 cut(s) 689, 1058, 1117, 1232, 1649, 1903
BmrFI CCNGG 5 cut(s) 285, 1156, 1283, 1292, 1823
BmrI ACTGGG 1 cut(s) 2257
BmsI GCATC 9 cut(s) 400, 535, 1141, 1327, 1354, 1664, 1852, 2231, 2284
BmuI ACTGGG 1 cut(s) 2257
BoxI GACNNNNGTC 1 cut(s) 1243
BpiI GAAGAC 2 cut(s) 829, 1419
BplI GAGNNNNNCTC 2 cut(s) 315, 347
BpmI CTGGAG 2 cut(s) 585, 1237
BpuEI CTTGAG 3 cut(s) 80, 89, 1522
BpuMI CCSGG 3 cut(s) 1283, 1292, 1823
BsaI GGTCTC 3 cut(s) 317, 1348, 2009
BsaJI CCNNGG 8 cut(s) 307, 1281, 1290, 1724, 1821, 1986, 1999, 2058
BsaWI WCCGGW 2 cut(s) 381, 1402
BsaXI ACNNNNNCTCC 2 cut(s) 1070, 1100
Bsc4I CCNNNNNNNGG 5 cut(s) 220, 308, 381, 832, 1360
Bse118I RCCGGY 1 cut(s) 1429
Bse1I ACTGG 8 cut(s) 144, 176, 411, 644, 929, 2171, 2178, 2252
Bse3DI GCAATG 1 cut(s) 813
BseAI TCCGGA 2 cut(s) 381, 1402
BseBI CCWGG 2 cut(s) 285, 1156
BseDI CCNNGG 8 cut(s) 307, 1281, 1290, 1724, 1821, 1986, 1999, 2058
BseLI CCNNNNNNNGG 5 cut(s) 220, 308, 381, 832, 1360
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 3 cut(s) 9, 328, 1371
BseNI ACTGG 8 cut(s) 144, 176, 411, 644, 929, 2171, 2178, 2252
BseSI GKGCMC 1 cut(s) 2240
BseXI GCAGC 3 cut(s) 1988, 2185, 2309
BsgI GTGCAG 2 cut(s) 1075, 1654
Bsh1236I CGCG 1 cut(s) 1811
Bsh1285I CGRYCG 1 cut(s) 2158
BshFI GGCC 6 cut(s) 74, 155, 690, 1139, 1429, 2148
BshNI GGYRCC 2 cut(s) 1647, 1901
BsiEI CGRYCG 1 cut(s) 2158
BsiHKAI GWGCWC 4 cut(s) 283, 729, 1606, 2240
BsiHKCI CYCGRG 1 cut(s) 1110
BsiSI CCGG 6 cut(s) 382, 1283, 1292, 1403, 1430, 1823
BslFI GGGAC 1 cut(s) 573
BslI CCNNNNNNNGG 5 cut(s) 220, 308, 381, 832, 1360
BsmAI GTCTC 7 cut(s) 43, 259, 317, 1215, 1348, 2009, 2128
BsmBI CGTCTC 1 cut(s) 2128
BsmFI GGGAC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 1879
BsnI GGCC 6 cut(s) 74, 155, 690, 1139, 1429, 2148
Bso31I GGTCTC 3 cut(s) 317, 1348, 2009
BsoBI CYCGRG 1 cut(s) 1110
Bsp1286I GDGCHC 4 cut(s) 283, 729, 1606, 2240
Bsp13I TCCGGA 2 cut(s) 381, 1402
Bsp1407I TGTACA 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 1986
BspACI CCGC 7 cut(s) 30, 1340, 1809, 1865, 1877, 2201, 2213
BspANI GGCC 6 cut(s) 74, 155, 690, 1139, 1429, 2148
BspCNI CTCAG 3 cut(s) 10, 327, 1370
BspEI TCCGGA 2 cut(s) 381, 1402
BspFNI CGCG 1 cut(s) 1811
BspHI TCATGA 1 cut(s) 789
BspLI GGNNCC 6 cut(s) 689, 1058, 1117, 1232, 1649, 1903
BspMI ACCTGC 2 cut(s) 1177, 2291
BspPI GGATC 7 cut(s) 320, 373, 1110, 1123, 1201, 1225, 1238
BspT107I GGYRCC 2 cut(s) 1647, 1901
BspTI CTTAAG 1 cut(s) 1565
BspTNI GGTCTC 3 cut(s) 317, 1348, 2009
BsrDI GCAATG 1 cut(s) 813
BsrFI RCCGGY 1 cut(s) 1429
BsrGI TGTACA 1 cut(s) 495
BsrI ACTGG 8 cut(s) 144, 176, 411, 644, 929, 2171, 2178, 2252
BssAI RCCGGY 1 cut(s) 1429
BssECI CCNNGG 8 cut(s) 307, 1281, 1290, 1724, 1821, 1986, 1999, 2058
BssNAI GTATAC 1 cut(s) 920
BssT1I CCWWGG 4 cut(s) 1724, 1986, 1999, 2058
Bst1107I GTATAC 1 cut(s) 920
Bst2UI CCWGG 2 cut(s) 285, 1156
Bst4CI ACNGT 2 cut(s) 495, 1245
Bst6I CTCTTC 3 cut(s) 350, 584, 1064
BstAFI CTTAAG 1 cut(s) 1565
BstAPI GCANNNNNTGC 2 cut(s) 818, 2235
BstAUI TGTACA 1 cut(s) 495
BstC8I GCNNGC 6 cut(s) 882, 950, 1141, 1431, 1602, 2146
BstDEI CTNAG 4 cut(s) 18, 314, 1357, 1920
BstDSI CCRYGG 2 cut(s) 307, 1986
BstFNI CGCG 1 cut(s) 1811
BstHHI GCGC 1 cut(s) 880
BstMAI GTCTC 7 cut(s) 43, 259, 317, 1215, 1348, 2009, 2128
BstMCI CGRYCG 1 cut(s) 2158
BstMWI GCNNNNNNNGC 9 cut(s) 818, 1010, 1373, 1382, 1874, 1982, 2195, 2219, 2235
BstNI CCWGG 2 cut(s) 285, 1156
BstNSI RCATGY 3 cut(s) 1709, 1961, 2189
BstPAI GACNNNNGTC 1 cut(s) 1243
BstSCI CCNGG 5 cut(s) 283, 1154, 1281, 1290, 1821
BstSLI GKGCMC 1 cut(s) 2240
BstUI CGCG 1 cut(s) 1811
BstV1I GCAGC 3 cut(s) 1988, 2185, 2309
BstV2I GAAGAC 2 cut(s) 829, 1419
BstX2I RGATCY 4 cut(s) 325, 1115, 1193, 1230
BstXI CCANNNNNNTGG 1 cut(s) 1147
BstYI RGATCY 4 cut(s) 325, 1115, 1193, 1230
BstZ17I GTATAC 1 cut(s) 920
BsuRI GGCC 6 cut(s) 74, 155, 690, 1139, 1429, 2148
BtgI CCRYGG 2 cut(s) 307, 1986
BtgZI GCGATG 1 cut(s) 1976
BtsIMutI CAGTG 4 cut(s) 491, 922, 1365, 1857
BveI ACCTGC 2 cut(s) 1177, 2291
Cac8I GCNNGC 6 cut(s) 882, 950, 1141, 1431, 1602, 2146
CciI TCATGA 1 cut(s) 789
CfoI GCGC 1 cut(s) 880
Cfr10I RCCGGY 1 cut(s) 1429
Cfr13I GGNCC 7 cut(s) 73, 434, 688, 1057, 1137, 2175, 2303
CseI GACGC 1 cut(s) 404
Csp6I GTAC 4 cut(s) 496, 509, 1064, 1902
CspCI CAANNNNNGTGG 2 cut(s) 200, 235
CviQI GTAC 4 cut(s) 496, 509, 1064, 1902
DdeI CTNAG 4 cut(s) 18, 314, 1357, 1920
DriI GACNNNNNGTC 1 cut(s) 2251
EaeI YGGCCR 2 cut(s) 1427, 2146
Eam1104I CTCTTC 3 cut(s) 350, 584, 1064
Eam1105I GACNNNNNGTC 1 cut(s) 2251
EarI CTCTTC 3 cut(s) 350, 584, 1064
Ecl136II GAGCTC 2 cut(s) 281, 1604
Eco130I CCWWGG 4 cut(s) 1724, 1986, 1999, 2058
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 2 cut(s) 283, 1606
Eco31I GGTCTC 3 cut(s) 317, 1348, 2009
Eco47I GGWCC 4 cut(s) 434, 1057, 2175, 2303
Eco53kI GAGCTC 2 cut(s) 281, 1604
Eco57I CTGAAG 2 cut(s) 218, 1088
Eco88I CYCGRG 1 cut(s) 1110
EcoICRI GAGCTC 2 cut(s) 281, 1604
EcoO109I RGGNCCY 1 cut(s) 2303
EcoRI GAATTC 1 cut(s) 2280
EcoRII CCWGG 2 cut(s) 283, 1154
EcoT14I CCWWGG 4 cut(s) 1724, 1986, 1999, 2058
EcoT38I GRGCYC 2 cut(s) 283, 1606
ErhI CCWWGG 4 cut(s) 1724, 1986, 1999, 2058
Esp3I CGTCTC 1 cut(s) 2128
FaqI GGGAC 1 cut(s) 573
FbaI TGATCA 2 cut(s) 792, 1846
FblI GTMKAC 4 cut(s) 46, 919, 1308, 2182
Fnu4HI GCNGC 5 cut(s) 1866, 1977, 2199, 2214, 2298
FriOI GRGCYC 2 cut(s) 283, 1606
Fsp4HI GCNGC 5 cut(s) 1866, 1977, 2199, 2214, 2298
GlaI GCGC 1 cut(s) 879
GluI GCNGC 5 cut(s) 1866, 1977, 2199, 2214, 2298
GsuI CTGGAG 2 cut(s) 585, 1237
HaeIII GGCC 6 cut(s) 74, 155, 690, 1139, 1429, 2148
HapII CCGG 6 cut(s) 382, 1283, 1292, 1403, 1430, 1823
HgaI GACGC 1 cut(s) 404
HhaI GCGC 1 cut(s) 880
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HincII GTYRAC 3 cut(s) 613, 1309, 2164
HindII GTYRAC 3 cut(s) 613, 1309, 2164
HpaI GTTAAC 2 cut(s) 613, 2164
HpaII CCGG 6 cut(s) 382, 1283, 1292, 1403, 1430, 1823
HphI GGTGA 4 cut(s) 80, 235, 1113, 1582
Hpy166II GTNNAC 9 cut(s) 47, 169, 613, 920, 1064, 1309, 2164, 2183, 2238
Hpy8I GTNNAC 9 cut(s) 47, 169, 613, 920, 1064, 1309, 2164, 2183, 2238
HpyAV CCTTC 4 cut(s) 858, 1016, 1105, 1684
HpyCH4III ACNGT 2 cut(s) 495, 1245
HpyCH4IV ACGT 1 cut(s) 1739
HpyF10VI GCNNNNNNNGC 9 cut(s) 818, 1010, 1373, 1382, 1874, 1982, 2195, 2219, 2235
HpyF3I CTNAG 4 cut(s) 18, 314, 1357, 1920
HpySE526I ACGT 1 cut(s) 1739
HspAI GCGC 1 cut(s) 878
Kpn2I TCCGGA 2 cut(s) 381, 1402
KpnI GGTACC 1 cut(s) 1905
KroI GCCGGC 1 cut(s) 1429
KroNI GCCGGC 1 cut(s) 1431
Ksp22I TGATCA 2 cut(s) 792, 1846
KspAI GTTAAC 2 cut(s) 613, 2164
LmnI GCTCC 3 cut(s) 184, 278, 286
Lsp1109I GCAGC 3 cut(s) 1988, 2185, 2309
LweI GCATC 9 cut(s) 400, 535, 1141, 1327, 1354, 1664, 1852, 2231, 2284
MaeII ACGT 1 cut(s) 1739
MaeIII GTNAC 6 cut(s) 99, 244, 392, 1076, 1570, 1783
MfeI CAATTG 3 cut(s) 499, 1225, 1698
MflI RGATCY 4 cut(s) 325, 1115, 1193, 1230
MhlI GDGCHC 4 cut(s) 283, 729, 1606, 2240
MlsI TGGCCA 1 cut(s) 2148
MluNI TGGCCA 1 cut(s) 2148
MlyI GAGTC 7 cut(s) 1081, 1304, 1409, 1481, 1567, 2048, 2198
MmeI TCCRAC 3 cut(s) 1004, 1171, 2025
Mox20I TGGCCA 1 cut(s) 2148
MroI TCCGGA 2 cut(s) 381, 1402
MroNI GCCGGC 1 cut(s) 1429
MroXI GAANNNNTTC 2 cut(s) 84, 1707
MscI TGGCCA 1 cut(s) 2148
MslI CAYNNNNRTG 2 cut(s) 68, 623
Msp20I TGGCCA 1 cut(s) 2148
MspCI CTTAAG 1 cut(s) 1565
MspI CCGG 6 cut(s) 382, 1283, 1292, 1403, 1430, 1823
MspR9I CCNGG 5 cut(s) 285, 1156, 1283, 1292, 1823
MunI CAATTG 3 cut(s) 499, 1225, 1698
Mva1269I GAATGC 1 cut(s) 1879
MvaI CCWGG 2 cut(s) 285, 1156
MvnI CGCG 1 cut(s) 1811
MwoI GCNNNNNNNGC 9 cut(s) 818, 1010, 1373, 1382, 1874, 1982, 2195, 2219, 2235
NaeI GCCGGC 1 cut(s) 1431
NciI CCSGG 3 cut(s) 1283, 1292, 1823
NcoI CCATGG 1 cut(s) 1986
NgoMIV GCCGGC 1 cut(s) 1429
NlaIV GGNNCC 6 cut(s) 689, 1058, 1117, 1232, 1649, 1903
NmuCI GTSAC 3 cut(s) 392, 1570, 1783
NspI RCATGY 3 cut(s) 1709, 1961, 2189
PagI TCATGA 1 cut(s) 789
PceI AGGCCT 1 cut(s) 155
PciI ACATGT 1 cut(s) 1705
PctI GAATGC 1 cut(s) 1879
PdiI GCCGGC 1 cut(s) 1431
PdmI GAANNNNTTC 2 cut(s) 84, 1707
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 5 cut(s) 1867, 1978, 2200, 2215, 2299
Ple19I CGATCG 1 cut(s) 2158
PleI GAGTC 7 cut(s) 1080, 1304, 1409, 1481, 1567, 2048, 2198
PpsI GAGTC 7 cut(s) 1080, 1304, 1409, 1481, 1567, 2048, 2198
PpuMI RGGWCCY 1 cut(s) 2303
PscI ACATGT 1 cut(s) 1705
PshAI GACNNNNGTC 1 cut(s) 1243
PshBI ATTAAT 1 cut(s) 558
Psp124BI GAGCTC 2 cut(s) 283, 1606
Psp5II RGGWCCY 1 cut(s) 2303
Psp6I CCWGG 2 cut(s) 283, 1154
PspGI CCWGG 2 cut(s) 283, 1154
PspN4I GGNNCC 6 cut(s) 689, 1058, 1117, 1232, 1649, 1903
PspPI GGNCC 7 cut(s) 73, 434, 688, 1057, 1137, 2175, 2303
PspPPI RGGWCCY 1 cut(s) 2303
PsuI RGATCY 4 cut(s) 325, 1115, 1193, 1230
PvuI CGATCG 1 cut(s) 2158
RsaI GTAC 4 cut(s) 497, 510, 1065, 1903
RsaNI GTAC 4 cut(s) 496, 509, 1064, 1902
RseI CAYNNNNRTG 2 cut(s) 68, 623
SacI GAGCTC 2 cut(s) 283, 1606
SalI GTCGAC 1 cut(s) 1307
SatI GCNGC 5 cut(s) 1866, 1977, 2199, 2214, 2298
Sau96I GGNCC 7 cut(s) 73, 434, 688, 1057, 1137, 2175, 2303
ScaI AGTACT 1 cut(s) 510
SchI GAGTC 7 cut(s) 1081, 1304, 1409, 1481, 1567, 2048, 2198
ScrFI CCNGG 5 cut(s) 285, 1156, 1283, 1292, 1823
SduI GDGCHC 4 cut(s) 283, 729, 1606, 2240
SfaNI GCATC 9 cut(s) 400, 535, 1141, 1327, 1354, 1664, 1852, 2231, 2284
SinI GGWCC 4 cut(s) 434, 1057, 2175, 2303
SmiMI CAYNNNNRTG 2 cut(s) 68, 623
SmlI CTYRAG 4 cut(s) 95, 104, 1537, 1565
SmoI CTYRAG 4 cut(s) 95, 104, 1537, 1565
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 7 cut(s) 30, 1340, 1809, 1865, 1877, 2201, 2213
SstI GAGCTC 2 cut(s) 283, 1606
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 5 cut(s) 283, 1154, 1281, 1290, 1821
StyI CCWWGG 4 cut(s) 1724, 1986, 1999, 2058
TaaI ACNGT 2 cut(s) 495, 1245
TaiI ACGT 1 cut(s) 1742
TaqI TCGA 5 cut(s) 732, 1308, 2096, 2134, 2154
TaqII GACCGA 1 cut(s) 823
TatI WGTACW 3 cut(s) 495, 508, 1063
TauI GCSGC 2 cut(s) 1868, 2216
TscAI CASTG 4 cut(s) 498, 929, 1365, 1864
TseFI GTSAC 3 cut(s) 392, 1570, 1783
TseI GCWGC 3 cut(s) 1976, 2198, 2297
Tsp45I GTSAC 3 cut(s) 392, 1570, 1783
TspDTI ATGAA 5 cut(s) 93, 161, 1425, 1485, 1499
TspGWI ACGGA 3 cut(s) 1049, 1444, 2255
TspRI CASTG 4 cut(s) 498, 929, 1365, 1864
Van91I CCANNNNNTGG 1 cut(s) 308
Vha464I CTTAAG 1 cut(s) 1565
VneI GTGCAC 1 cut(s) 2236
VpaK11BI GGWCC 4 cut(s) 434, 1057, 2175, 2303
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 6 cut(s) 125, 293, 741, 842, 886, 2280
XceI RCATGY 3 cut(s) 1709, 1961, 2189
XcmI CCANNNNNNNNNTGG 1 cut(s) 1131
XmiI GTMKAC 4 cut(s) 46, 919, 1308, 2182
XmnI GAANNNNTTC 2 cut(s) 84, 1707
ZrmI AGTACT 1 cut(s) 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.