ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 502 |
| Acc36I | ACCTGC | 2 cut(s) | 319, 502 |
| Acc65I | GGTACC | 1 cut(s) | 556 |
| AccB1I | GGYRCC | 1 cut(s) | 556 |
| AcsI | RAATTY | 1 cut(s) | 184 |
| AcuI | CTGAAG | 1 cut(s) | 21 |
| AfaI | GTAC | 3 cut(s) | 158, 309, 558 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 139, 622 |
| AgsI | TTSAA | 2 cut(s) | 110, 239 |
| AjnI | CCWGG | 1 cut(s) | 666 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 455, 487 |
| AluBI | AGCT | 9 cut(s) | 35, 98, 235, 359, 381, 398, 631, 687, 728 |
| AluI | AGCT | 9 cut(s) | 35, 98, 235, 359, 381, 398, 631, 687, 728 |
| Alw21I | GWGCWC | 1 cut(s) | 689 |
| Alw26I | GTCTC | 2 cut(s) | 170, 473 |
| Ama87I | CYCGRG | 3 cut(s) | 56, 148, 729 |
| AoxI | GGCC | 4 cut(s) | 121, 446, 708, 752 |
| ApoI | RAATTY | 1 cut(s) | 184 |
| Asp718I | GGTACC | 1 cut(s) | 556 |
| AspS9I | GGNCC | 2 cut(s) | 145, 671 |
| AsuC2I | CCSGG | 2 cut(s) | 149, 150 |
| AsuHPI | GGTGA | 2 cut(s) | 526, 734 |
| AsuNHI | GCTAGC | 1 cut(s) | 377 |
| AvaI | CYCGRG | 3 cut(s) | 56, 148, 729 |
| AvaII | GGWCC | 2 cut(s) | 145, 671 |
| BanI | GGYRCC | 1 cut(s) | 556 |
| BanII | GRGCYC | 1 cut(s) | 689 |
| Bbv12I | GWGCWC | 1 cut(s) | 689 |
| BccI | CCATC | 1 cut(s) | 457 |
| BciT130I | CCWGG | 1 cut(s) | 668 |
| BclI | TGATCA | 1 cut(s) | 579 |
| BcnI | CCSGG | 2 cut(s) | 149, 150 |
| BcoDI | GTCTC | 2 cut(s) | 170, 473 |
| BfaI | CTAG | 4 cut(s) | 95, 378, 399, 444 |
| BfuAI | ACCTGC | 2 cut(s) | 319, 502 |
| BlpI | GCTNAGC | 1 cut(s) | 627 |
| Bme1390I | CCNGG | 3 cut(s) | 149, 150, 668 |
| Bme18I | GGWCC | 2 cut(s) | 145, 671 |
| BmeT110I | CYCGRG | 3 cut(s) | 56, 148, 729 |
| BmgT120I | GGNCC | 2 cut(s) | 145, 671 |
| BmiI | GGNNCC | 3 cut(s) | 147, 558, 672 |
| BmrFI | CCNGG | 3 cut(s) | 149, 150, 668 |
| BmtI | GCTAGC | 1 cut(s) | 381 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 627 |
| BpuMI | CCSGG | 2 cut(s) | 149, 150 |
| BsaI | GGTCTC | 1 cut(s) | 170 |
| BsaJI | CCNNGG | 5 cut(s) | 55, 148, 160, 300, 667 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 73, 103 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 139, 622 |
| Bse3DI | GCAATG | 1 cut(s) | 526 |
| BseBI | CCWGG | 1 cut(s) | 668 |
| BseDI | CCNNGG | 5 cut(s) | 55, 148, 160, 300, 667 |
| BseGI | GGATG | 1 cut(s) | 65 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 139, 622 |
| BseMI | GCAATG | 1 cut(s) | 526 |
| BseMII | CTCAG | 2 cut(s) | 278, 618 |
| BseRI | GAGGAG | 1 cut(s) | 81 |
| BshFI | GGCC | 4 cut(s) | 123, 448, 710, 754 |
| BshNI | GGYRCC | 1 cut(s) | 556 |
| BsiHKAI | GWGCWC | 1 cut(s) | 689 |
| BsiHKCI | CYCGRG | 3 cut(s) | 56, 148, 729 |
| BsiSI | CCGG | 1 cut(s) | 149 |
| BslFI | GGGAC | 1 cut(s) | 684 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 139, 622 |
| BsmAI | GTCTC | 2 cut(s) | 170, 473 |
| BsmFI | GGGAC | 1 cut(s) | 684 |
| BsmI | GAATGC | 1 cut(s) | 503 |
| BsnI | GGCC | 4 cut(s) | 123, 448, 710, 754 |
| Bso31I | GGTCTC | 1 cut(s) | 170 |
| BsoBI | CYCGRG | 3 cut(s) | 56, 148, 729 |
| Bsp1286I | GDGCHC | 1 cut(s) | 689 |
| Bsp143I | GATC | 1 cut(s) | 579 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 627 |
| Bsp19I | CCATGG | 1 cut(s) | 300 |
| BspANI | GGCC | 4 cut(s) | 123, 448, 710, 754 |
| BspCNI | CTCAG | 2 cut(s) | 277, 619 |
| BspLI | GGNNCC | 3 cut(s) | 147, 558, 672 |
| BspMI | ACCTGC | 2 cut(s) | 319, 502 |
| BspOI | GCTAGC | 1 cut(s) | 381 |
| BspT107I | GGYRCC | 1 cut(s) | 556 |
| BspTNI | GGTCTC | 1 cut(s) | 170 |
| BsrDI | GCAATG | 1 cut(s) | 526 |
| BssECI | CCNNGG | 5 cut(s) | 55, 148, 160, 300, 667 |
| BssMI | GATC | 1 cut(s) | 579 |
| BssT1I | CCWWGG | 2 cut(s) | 160, 300 |
| Bst2UI | CCWGG | 1 cut(s) | 668 |
| Bst4CI | ACNGT | 2 cut(s) | 29, 781 |
| Bst6I | CTCTTC | 2 cut(s) | 268, 475 |
| BstC8I | GCNNGC | 1 cut(s) | 379 |
| BstDEI | CTNAG | 2 cut(s) | 264, 627 |
| BstDSI | CCRYGG | 1 cut(s) | 300 |
| BstF5I | GGATG | 1 cut(s) | 65 |
| BstKTI | GATC | 1 cut(s) | 582 |
| BstMAI | GTCTC | 2 cut(s) | 170, 473 |
| BstMBI | GATC | 1 cut(s) | 579 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 707 |
| BstNI | CCWGG | 1 cut(s) | 668 |
| BstSCI | CCNGG | 3 cut(s) | 147, 148, 666 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 177 |
| BsuRI | GGCC | 4 cut(s) | 123, 448, 710, 754 |
| BtgI | CCRYGG | 1 cut(s) | 300 |
| BtsCI | GGATG | 1 cut(s) | 65 |
| BtsIMutI | CAGTG | 1 cut(s) | 777 |
| BveI | ACCTGC | 2 cut(s) | 319, 502 |
| Cac8I | GCNNGC | 1 cut(s) | 379 |
| Cfr13I | GGNCC | 2 cut(s) | 145, 671 |
| Cfr9I | CCCGGG | 1 cut(s) | 148 |
| Csp6I | GTAC | 3 cut(s) | 157, 308, 557 |
| CviAII | CATG | 3 cut(s) | 301, 337, 351 |
| CviQI | GTAC | 3 cut(s) | 157, 308, 557 |
| DdeI | CTNAG | 2 cut(s) | 264, 627 |
| DpnI | GATC | 1 cut(s) | 581 |
| DpnII | GATC | 1 cut(s) | 579 |
| Eam1104I | CTCTTC | 2 cut(s) | 268, 475 |
| EarI | CTCTTC | 2 cut(s) | 268, 475 |
| Ecl136II | GAGCTC | 1 cut(s) | 687 |
| Eco130I | CCWWGG | 2 cut(s) | 160, 300 |
| Eco147I | AGGCCT | 1 cut(s) | 123 |
| Eco24I | GRGCYC | 1 cut(s) | 689 |
| Eco31I | GGTCTC | 1 cut(s) | 170 |
| Eco32I | GATATC | 1 cut(s) | 138 |
| Eco47I | GGWCC | 2 cut(s) | 145, 671 |
| Eco53kI | GAGCTC | 1 cut(s) | 687 |
| Eco57I | CTGAAG | 1 cut(s) | 21 |
| Eco88I | CYCGRG | 3 cut(s) | 56, 148, 729 |
| EcoICRI | GAGCTC | 1 cut(s) | 687 |
| EcoO109I | RGGNCCY | 2 cut(s) | 145, 671 |
| EcoRII | CCWGG | 1 cut(s) | 666 |
| EcoRV | GATATC | 1 cut(s) | 138 |
| EcoT14I | CCWWGG | 2 cut(s) | 160, 300 |
| EcoT38I | GRGCYC | 1 cut(s) | 689 |
| ErhI | CCWWGG | 2 cut(s) | 160, 300 |
| FaeI | CATG | 3 cut(s) | 304, 340, 354 |
| FaiI | YATR | 6 cut(s) | 208, 302, 338, 352, 619, 621 |
| FaqI | GGGAC | 1 cut(s) | 684 |
| FatI | CATG | 3 cut(s) | 300, 336, 350 |
| FbaI | TGATCA | 1 cut(s) | 579 |
| FokI | GGATG | 1 cut(s) | 52 |
| FriOI | GRGCYC | 1 cut(s) | 689 |
| FspBI | CTAG | 4 cut(s) | 95, 378, 399, 444 |
| HaeIII | GGCC | 4 cut(s) | 123, 448, 710, 754 |
| HapII | CCGG | 1 cut(s) | 149 |
| Hin1II | CATG | 3 cut(s) | 304, 340, 354 |
| HincII | GTYRAC | 1 cut(s) | 535 |
| HindII | GTYRAC | 1 cut(s) | 535 |
| HindIII | AAGCTT | 2 cut(s) | 33, 233 |
| HinfI | GANTC | 1 cut(s) | 388 |
| HpaII | CCGG | 1 cut(s) | 149 |
| HphI | GGTGA | 2 cut(s) | 526, 734 |
| Hpy166II | GTNNAC | 2 cut(s) | 52, 535 |
| Hpy188I | TCNGA | 4 cut(s) | 142, 370, 426, 691 |
| Hpy188III | TCNNGA | 6 cut(s) | 58, 133, 283, 385, 541, 731 |
| Hpy8I | GTNNAC | 2 cut(s) | 52, 535 |
| HpyAV | CCTTC | 5 cut(s) | 113, 190, 233, 241, 421 |
| HpyCH4III | ACNGT | 2 cut(s) | 29, 781 |
| HpyCH4IV | ACGT | 2 cut(s) | 155, 693 |
| HpyCH4V | TGCA | 2 cut(s) | 206, 354 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 707 |
| HpyF3I | CTNAG | 2 cut(s) | 264, 627 |
| HpySE526I | ACGT | 2 cut(s) | 155, 693 |
| Hsp92II | CATG | 3 cut(s) | 304, 340, 354 |
| KpnI | GGTACC | 1 cut(s) | 560 |
| Ksp22I | TGATCA | 1 cut(s) | 579 |
| Kzo9I | GATC | 1 cut(s) | 579 |
| LmnI | GCTCC | 1 cut(s) | 654 |
| MaeI | CTAG | 4 cut(s) | 95, 378, 399, 444 |
| MaeII | ACGT | 2 cut(s) | 155, 693 |
| MalI | GATC | 1 cut(s) | 581 |
| MboI | GATC | 1 cut(s) | 579 |
| MboII | GAAGA | 4 cut(s) | 67, 285, 492, 746 |
| MfeI | CAATTG | 1 cut(s) | 717 |
| MhlI | GDGCHC | 1 cut(s) | 689 |
| MluCI | AATT | 7 cut(s) | 44, 105, 111, 184, 222, 633, 717 |
| MmeI | TCCRAC | 2 cut(s) | 224, 632 |
| MnlI | CCTC | 5 cut(s) | 65, 102, 134, 136, 358 |
| MseI | TTAA | 4 cut(s) | 5, 225, 288, 705 |
| MspI | CCGG | 1 cut(s) | 149 |
| MspR9I | CCNGG | 3 cut(s) | 149, 150, 668 |
| MunI | CAATTG | 1 cut(s) | 717 |
| Mva1269I | GAATGC | 1 cut(s) | 503 |
| MvaI | CCWGG | 1 cut(s) | 668 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 707 |
| NciI | CCSGG | 2 cut(s) | 149, 150 |
| NcoI | CCATGG | 1 cut(s) | 300 |
| NdeII | GATC | 1 cut(s) | 579 |
| NheI | GCTAGC | 1 cut(s) | 377 |
| NlaIII | CATG | 3 cut(s) | 304, 340, 354 |
| NlaIV | GGNNCC | 3 cut(s) | 147, 558, 672 |
| PaqCI | CACCTGC | 1 cut(s) | 502 |
| PceI | AGGCCT | 1 cut(s) | 123 |
| PctI | GAATGC | 1 cut(s) | 503 |
| PfeI | GAWTC | 1 cut(s) | 388 |
| PpuMI | RGGWCCY | 2 cut(s) | 145, 671 |
| Psp124BI | GAGCTC | 1 cut(s) | 689 |
| Psp5II | RGGWCCY | 2 cut(s) | 145, 671 |
| Psp6I | CCWGG | 1 cut(s) | 666 |
| PspGI | CCWGG | 1 cut(s) | 666 |
| PspN4I | GGNNCC | 3 cut(s) | 147, 558, 672 |
| PspPI | GGNCC | 2 cut(s) | 145, 671 |
| PspPPI | RGGWCCY | 2 cut(s) | 145, 671 |
| RsaI | GTAC | 3 cut(s) | 158, 309, 558 |
| RsaNI | GTAC | 3 cut(s) | 157, 308, 557 |
| SacI | GAGCTC | 1 cut(s) | 689 |
| SaqAI | TTAA | 4 cut(s) | 5, 225, 288, 705 |
| Sau3AI | GATC | 1 cut(s) | 579 |
| Sau96I | GGNCC | 2 cut(s) | 145, 671 |
| ScrFI | CCNGG | 3 cut(s) | 149, 150, 668 |
| SduI | GDGCHC | 1 cut(s) | 689 |
| SinI | GGWCC | 2 cut(s) | 145, 671 |
| SmaI | CCCGGG | 1 cut(s) | 150 |
| Sse9I | AATT | 7 cut(s) | 44, 105, 111, 184, 222, 633, 717 |
| SseBI | AGGCCT | 1 cut(s) | 123 |
| SspMI | CTAG | 4 cut(s) | 95, 378, 399, 444 |
| SstI | GAGCTC | 1 cut(s) | 689 |
| StuI | AGGCCT | 1 cut(s) | 123 |
| StyD4I | CCNGG | 3 cut(s) | 147, 148, 666 |
| StyI | CCWWGG | 2 cut(s) | 160, 300 |
| TaaI | ACNGT | 2 cut(s) | 29, 781 |
| TaiI | ACGT | 2 cut(s) | 158, 696 |
| TaqI | TCGA | 2 cut(s) | 320, 417 |
| TasI | AATT | 7 cut(s) | 44, 105, 111, 184, 222, 633, 717 |
| TfiI | GAWTC | 1 cut(s) | 388 |
| Tru1I | TTAA | 4 cut(s) | 5, 225, 288, 705 |
| Tru9I | TTAA | 4 cut(s) | 5, 225, 288, 705 |
| TscAI | CASTG | 1 cut(s) | 784 |
| TspMI | CCCGGG | 1 cut(s) | 148 |
| TspRI | CASTG | 1 cut(s) | 784 |
| VpaK11BI | GGWCC | 2 cut(s) | 145, 671 |
| XapI | RAATTY | 1 cut(s) | 184 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 456 |
| XmaI | CCCGGG | 1 cut(s) | 148 |
| XspI | CTAG | 4 cut(s) | 95, 378, 399, 444 |