pycom15g31740

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000194
Physical Location & Seq
Forward (+)
30362400 .. 30364274
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g31740.1

Sequence Viewer

Length: 1782 bp
ATGGTTGAGGGAGGGGGATGCAAACACTCAGTTTTTTCACTCTTCAACCCTTCAAGGCGTAGGAGAAATAAGATTGTGAAGCTTAGGGCTGAAAATGGAAACTGGGTGGAAAATCCGTCTCAGGTGCGCCATTTAGTGGATAATCATTTCTCTTCAGTTTTTAGCTCGGCAGGGGATCGTAACTGGGGGTCGCTGCTAGATTGCATTAATCCCTCGGTCTCGACAGAAATGAATGAGGCGCTAACTGCACCGGTTACGGATGAGGAGATAAAGGAGGCTGCTGGGAATATGGGAGGTCTTAAGGCTCCCGACCCCGATGGTTTCCAGGGGATCTTCTATCAGACATACTGGGAGATTGTGAAGGAAGATGTTTCGGCTTTGGTCAGAGAGTTGCTTCAGGATGTCGCGGGTTCGAGATTGATAAATCAGACTAATATAGTTATGATTCCCAAAGTCCCTAATCCGGAATTTGTGTCGCAGTTCAGGCCTATCAGCCTGTGTAACTACTCGTACAAAATACTGTCAAAGATTCTTGCTAACCGGTTGAAGGTATTATTGCCTAAAATCATATCTCCATCTCAGAATGCTTTTGTGGTGGGTAGACAAATTCAGGATTGCATTAGCATAGCCCATGAGATGTTTCATTATCTGAAGGGAAGGAAAGTCAGGAACAGATTTGAAATGGGGATTAAACTGGATATGCAGAAGGCGTATGATAGGGTTGAATGGGACTTTTTGGATGCGGCTGGAGAGTCTTTTGCTCCCTCGCGGGGTCTCCGTCAGGGGGATCCTCTTTCTCCTTATTTATTTATTTTGGTGGGGGAAGTTCTTTCCAAACTGATTCAGGGTGCGGTGGACCAAGGTAGGTTGGAGGGTGTTAAGATTGGTGGATCCGGACCCGTGATTTCTCACCTCTTCTTTGCGGATGATACCCTTCTGTTTCTGCGGGCAGATGTGAAAAACTGCAGGAATTTGAAACATATGCTGGACAAATTTTGTGTGGCGTCTGGGCAGAAGGTTAATTTGGAGAAATCCAGTGTTTTCTTTGGGGCAAATGTTCCGAATGTGAACGCGGATCAGATGGGGAATGCCCTTGGTATGACGGTGGTTAGCAATCCGGGGACTTATTTGGGGGTCCCTGCTATTTGGGGCCGGTCGAAAAAGCGTGGTCTTGCTTATGTGAAAGGAAGAGTAATGGAAAAGCTTCAAGGGTGGAAACAAAGTACTCTATCCCGTGCCGGTAAAGAGGTTTTAATTAAGGCTGTTATCCAAGCTATTCCGGCGTACCCAATGTGCATTTTCAAATTCCCCGCCGTCGTTTGTCAGGAATTGGATGCGTTAGTGGCTGGATTTTGGTGGGGTTGCAAGGAGGGAGCCCAAAAGATTCATTGGGTCTCAAAGGAGGTTTTGGGTTTGCCAAAAGACTTGGGTGGTTTAGGCTTCAGAAACTTTATGGAATTTAATGATGCTCTGCTTGCTAAACAATGTTGGCGTTTAATCACGGAACCGGACTCGCTGTGGGCTAGGGTGATGAAAGCCCGGTATTTTCCCCATTGCTCGATATGGGATGCGAAGAAAGGTGGTCGGGCTTCTTGGGCATGGAGCAGCCTCATCTGTGGCAGGGACGTGATCAAGGAGGGTTCCCATTGGCAGATTTTGGGCGGTCAGGAGGTGAGGGTGTGGCAGGATAGGTGGCTTCCATCCTTGCCCCTTGGACATCCGGTGCCGGTTGGCCAGGTTGCGGTTACTCCCAGTTTAAGGGTCAGTGCGCTTATCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-CCHC_4 zf-RVT

Protein Analysis

594

Amino Acids

66.06

Weight (kDa)

9.3

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 153 - 379 3.9e-25 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000194)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34320 AT4G29090 AT5G42965
fragaria_vesca FvH4_1g19471 FvH4_1g29471 FvH4_1g29601 FvH4_2g04611 FvH4_2g05763 FvH4_2g11451 FvH4_2g11841 FvH4_3g10391 FvH4_3g21491 FvH4_3g21492 FvH4_3g32111 FvH4_3g35051 FvH4_4g06793 FvH4_5g08844 FvH4_5g15942 FvH4_5g18751 FvH4_5g27367 FvH4_6g11612 FvH4_6g20242 FvH4_6g27671 FvH4_6g31921 FvH4_7g32463
malus_domestica MD06G1107000.v1.1 MD07G1135800.v1.1 MD08G1205700.v1.1
prunus_persica Prupe.1G202000_v2.0.a1 Prupe.2G049100_v2.0.a1 Prupe.2G060500_v2.0.a1 Prupe.3G120300_v2.0.a1 Prupe.3G135000_v2.0.a1 Prupe.6G087800_v2.0.a1 Prupe.6G158500_v2.0.a1 Prupe.6G353900_v2.0.a1 Prupe.7G008600_v2.0.a1 Prupe.8G090500_v2.0.a1
pyrus_communis pycom01g21690 pycom02g19910 pycom04g18080 pycom07g08600 pycom07g18710 pycom07g26560 pycom08g03130 pycom08g13950 pycom09g06380 pycom10g13390 pycom10g22280 pycom11g04440 pycom11g10940 pycom12g04710 pycom13g08790 pycom15g31740 pycom17g14740 pycom17g24980
rosa_chinensis RchiOBHm_Chr1g0327801 RchiOBHm_Chr1g0347181 RchiOBHm_Chr1g0348821 RchiOBHm_Chr2g0157831 RchiOBHm_Chr5g0040851 RchiOBHm_Chr6g0249781 RchiOBHm_Chr7g0208461 RchiOBHm_Chr7g0210911 RchiOBHm_Chr7g0219141
rosa_laevigata RLG00000034326
rosa_multiflora Rmu_co8140276.1_g000001 Rmu_co8282095.1_g000001 Rmu_co8303311.1_g000001 Rmu_co8314215.1_g000001 Rmu_co8345435.1_g000001 Rmu_co8461745.1_g000001 Rmu_sc0000315.1_g000040 Rmu_sc0000487.1_g000014 Rmu_sc0000690.1_g000002 Rmu_sc0000808.1_g000001 Rmu_sc0000938.1_g000009 Rmu_sc0001260.1_g000002 Rmu_sc0001534.1_g000008 Rmu_sc0001669.1_g000003 Rmu_sc0001685.1_g000050 Rmu_sc0001838.1_g000007 Rmu_sc0002193.1_g000025 Rmu_sc0002308.1_g000058 Rmu_sc0002322.1_g000018 Rmu_sc0002529.1_g000008 Rmu_sc0002531.1_g000043 Rmu_sc0002754.1_g000022 Rmu_sc0002902.1_g000055 Rmu_sc0003627.1_g000005 Rmu_sc0003743.1_g000003 Rmu_sc0003765.1_g000016 Rmu_sc0003941.1_g000020 Rmu_sc0004038.1_g000011 Rmu_sc0004329.1_g000010 Rmu_sc0004476.1_g000002 Rmu_sc0004904.1_g000022 Rmu_sc0004988.1_g000017 Rmu_sc0005217.1_g000006 Rmu_sc0005257.1_g000010 Rmu_sc0005285.1_g000008 Rmu_sc0005687.1_g000001 Rmu_sc0006380.1_g000020 Rmu_sc0006922.1_g000004 Rmu_sc0007398.1_g000007 Rmu_sc0008279.1_g000019 Rmu_sc0009139.1_g000004 Rmu_sc0009158.1_g000001 Rmu_sc0010099.1_g000002 Rmu_sc0019043.1_g000001 Rmu_sc0022037.1_g000001 Rmu_sc0023291.1_g000001 Rmu_sc0031928.1_g000001 Rmu_sc0034436.1_g000002 Rmu_ssc0000126.1_g000001 Rmu_ssc0000151.1_g000016 Rmu_ssc0000152.1_g000009 Rmu_ssc0000395.1_g000031
rosa_roxburghii Rroxscaffold_1G00056570 Rroxscaffold_1G00058460 Rroxscaffold_2G00106120 Rroxscaffold_2G00106260 Rroxscaffold_6G00399080
rosa_rugosa Rorug02G0173300 Rorug02G0225600 Rorug03G0083300 Rorug03G0104900 Rorug04G0256500 Rorug05G0342600 Rorug06G0006200 Rorug06G0095200 Rorug06G0095600 Rorug06G0111500 Rorug07G0001200 Rorug07G0183100 Rorug07G0183100 Rorug07G0209000
rosa_samantha Rh2DG065400 Rh3DG315900 Rh4AG106100 Rh4BG362100 Rh4CG030200 Rh4DG239000 Rh4DG294000 Rh6DG304800 Rh7DG149500
rosa_wichuraiana Rw1G018070 Rw1G021220 Rw1G021820 Rw1G026430 Rw2G050800 Rw3G023330 Rw6G010050 Rw6G010440 Rw7G031930 Rw7G033860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1723
AccB7I CCANNNNNTGG 1 cut(s) 136
AccI GTMKAC 1 cut(s) 601
AccII CGCG 3 cut(s) 407, 769, 1073
AccIII TCCGGA 2 cut(s) 463, 893
AclWI GGATC 7 cut(s) 183, 338, 782, 795, 885, 898, 1083
AcoI YGGCCR 1 cut(s) 1732
AcsI RAATTY 6 cut(s) 467, 606, 970, 992, 1304, 1457
AcuI CTGAAG 4 cut(s) 138, 380, 671, 1426
AcyI GRCGYC 1 cut(s) 1004
AfaI GTAC 3 cut(s) 512, 1225, 1286
AfiI CCNNNNNNNGG 7 cut(s) 136, 463, 547, 770, 784, 1741, 1758
AflII CTTAAG 1 cut(s) 299
AgeI ACCGGT 2 cut(s) 250, 540
AgsI TTSAA 8 cut(s) 46, 54, 547, 680, 725, 976, 1208, 1303
AjiI CACGTC 1 cut(s) 1627
AjnI CCWGG 2 cut(s) 324, 1734
AjuI GAANNNNNNNTTGG 2 cut(s) 1007, 1039
AluBI AGCT 4 cut(s) 82, 165, 1204, 1274
AluI AGCT 4 cut(s) 82, 165, 1204, 1274
Alw26I GTCTC 4 cut(s) 123, 223, 779, 1399
AlwI GGATC 7 cut(s) 183, 338, 782, 795, 885, 898, 1083
Aor13HI TCCGGA 2 cut(s) 463, 893
AoxI GGCC 3 cut(s) 485, 1150, 1732
ApeKI GCWGC 3 cut(s) 193, 278, 1605
ApoI RAATTY 6 cut(s) 467, 606, 970, 992, 1304, 1457
AseI ATTAAT 1 cut(s) 207
AsiGI ACCGGT 2 cut(s) 250, 540
Asp700I GAANNNNTTC 1 cut(s) 1203
AspLEI GCGC 3 cut(s) 129, 241, 1771
AspS9I GGNCC 4 cut(s) 856, 896, 1135, 1150
AsuC2I CCSGG 2 cut(s) 1119, 1540
AsuHPI GGTGA 3 cut(s) 902, 1540, 1684
AvaII GGWCC 3 cut(s) 856, 896, 1135
BalI TGGCCA 1 cut(s) 1734
BamHI GGATCC 2 cut(s) 787, 890
BanI GGYRCC 1 cut(s) 1723
BanII GRGCYC 1 cut(s) 1378
BbvI GCAGC 3 cut(s) 180, 265, 1617
BccI CCATC 4 cut(s) 311, 583, 1075, 1708
BceAI ACGGC 1 cut(s) 1298
BciT130I CCWGG 2 cut(s) 326, 1736
BclI TGATCA 1 cut(s) 1629
BcnI CCSGG 2 cut(s) 1119, 1540
BcoDI GTCTC 4 cut(s) 123, 223, 779, 1399
BfaI CTAG 2 cut(s) 197, 1524
BfmI CTRYAG 1 cut(s) 964
BfoI RGCGCY 1 cut(s) 242
BfrI CTTAAG 1 cut(s) 299
BisI GCNGC 4 cut(s) 194, 279, 744, 1606
BlsI GCNGC 4 cut(s) 195, 280, 745, 1607
BmcAI AGTACT 1 cut(s) 1225
Bme1390I CCNGG 4 cut(s) 326, 1119, 1540, 1736
Bme18I GGWCC 3 cut(s) 856, 896, 1135
BmgBI CACGTC 1 cut(s) 1627
BmgT120I GGNCC 4 cut(s) 856, 896, 1135, 1150
BmrFI CCNGG 4 cut(s) 326, 1119, 1540, 1736
BmrI ACTGGG 4 cut(s) 112, 193, 358, 1746
BmsI GCATC 5 cut(s) 8, 730, 1324, 1456, 1558
BmuI ACTGGG 4 cut(s) 112, 193, 358, 1746
BpmI CTGGAG 1 cut(s) 768
Bpu10I CCTNAGC 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 1119, 1540
BsaHI GRCGYC 1 cut(s) 1004
BsaI GGTCTC 3 cut(s) 223, 779, 1399
BsaJI CCNNGG 6 cut(s) 213, 325, 859, 1093, 1118, 1711
BsaWI WCCGGW 6 cut(s) 250, 463, 540, 893, 1507, 1720
Bsc4I CCNNNNNNNGG 7 cut(s) 136, 463, 547, 770, 784, 1741, 1758
Bse118I RCCGGY 5 cut(s) 250, 540, 1152, 1238, 1726
Bse1I ACTGG 6 cut(s) 107, 188, 353, 699, 1035, 1752
Bse3DI GCAATG 1 cut(s) 1552
BseAI TCCGGA 2 cut(s) 463, 893
BseBI CCWGG 2 cut(s) 326, 1736
BseDI CCNNGG 6 cut(s) 213, 325, 859, 1093, 1118, 1711
BseGI GGATG 9 cut(s) 23, 265, 406, 745, 931, 1339, 1573, 1700, 1717
BseLI CCNNNNNNNGG 7 cut(s) 136, 463, 547, 770, 784, 1741, 1758
BseMI GCAATG 1 cut(s) 1552
BseMII CTCAG 3 cut(s) 42, 134, 593
BseNI ACTGG 6 cut(s) 107, 188, 353, 699, 1035, 1752
BseRI GAGGAG 1 cut(s) 278
BseXI GCAGC 3 cut(s) 180, 265, 1617
BseYI CCCAGC 1 cut(s) 281
BsgI GTGCAG 1 cut(s) 231
Bsh1236I CGCG 3 cut(s) 407, 769, 1073
Bsh1285I CGRYCG 1 cut(s) 1157
BshFI GGCC 3 cut(s) 487, 1152, 1734
BshNI GGYRCC 1 cut(s) 1723
BshTI ACCGGT 2 cut(s) 250, 540
BsiEI CGRYCG 1 cut(s) 1157
BslFI GGGAC 5 cut(s) 440, 743, 1121, 1135, 1637
BslI CCNNNNNNNGG 7 cut(s) 136, 463, 547, 770, 784, 1741, 1758
BsmAI GTCTC 4 cut(s) 123, 223, 779, 1399
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 5 cut(s) 440, 743, 1121, 1135, 1637
BsmI GAATGC 2 cut(s) 589, 1093
BsnI GGCC 3 cut(s) 487, 1152, 1734
Bso31I GGTCTC 3 cut(s) 223, 779, 1399
Bsp1286I GDGCHC 1 cut(s) 1378
Bsp13I TCCGGA 2 cut(s) 463, 893
Bsp143I GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
BspANI GGCC 3 cut(s) 487, 1152, 1734
BspCNI CTCAG 3 cut(s) 41, 133, 592
BspEI TCCGGA 2 cut(s) 463, 893
BspFNI CGCG 3 cut(s) 407, 769, 1073
BspMAI CTGCAG 1 cut(s) 968
BspPI GGATC 7 cut(s) 183, 338, 782, 795, 885, 898, 1083
BspT107I GGYRCC 1 cut(s) 1723
BspTI CTTAAG 1 cut(s) 299
BspTNI GGTCTC 3 cut(s) 223, 779, 1399
BsrDI GCAATG 1 cut(s) 1552
BsrFI RCCGGY 5 cut(s) 250, 540, 1152, 1238, 1726
BsrI ACTGG 6 cut(s) 107, 188, 353, 699, 1035, 1752
BssAI RCCGGY 5 cut(s) 250, 540, 1152, 1238, 1726
BssECI CCNNGG 6 cut(s) 213, 325, 859, 1093, 1118, 1711
BssMI GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
BssNI GRCGYC 1 cut(s) 1004
BssT1I CCWWGG 3 cut(s) 859, 1093, 1711
Bst2UI CCWGG 2 cut(s) 326, 1736
Bst4CI ACNGT 2 cut(s) 522, 1105
Bst6I CTCTTC 4 cut(s) 47, 157, 920, 1183
BstACI GRCGYC 1 cut(s) 1004
BstAFI CTTAAG 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 948, 1476
BstDEI CTNAG 4 cut(s) 28, 83, 120, 579
BstF5I GGATG 9 cut(s) 23, 265, 406, 745, 931, 1339, 1573, 1700, 1717
BstFNI CGCG 3 cut(s) 407, 769, 1073
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 3 cut(s) 129, 241, 1771
BstKTI GATC 6 cut(s) 178, 333, 790, 893, 1078, 1632
BstMAI GTCTC 4 cut(s) 123, 223, 779, 1399
BstMBI GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
BstMCI CGRYCG 1 cut(s) 1157
BstMWI GCNNNNNNNGC 6 cut(s) 245, 484, 1280, 1343, 1475, 1595
BstNI CCWGG 2 cut(s) 326, 1736
BstSCI CCNGG 4 cut(s) 324, 1117, 1538, 1734
BstSFI CTRYAG 1 cut(s) 964
BstUI CGCG 3 cut(s) 407, 769, 1073
BstV1I GCAGC 3 cut(s) 180, 265, 1617
BstX2I RGATCY 3 cut(s) 330, 787, 890
BstYI RGATCY 3 cut(s) 330, 787, 890
BsuRI GGCC 3 cut(s) 487, 1152, 1734
BtrI CACGTC 1 cut(s) 1627
BtsCI GGATG 9 cut(s) 23, 265, 406, 745, 931, 1339, 1573, 1700, 1717
BtsIMutI CAGTG 2 cut(s) 1042, 1771
Cac8I GCNNGC 2 cut(s) 948, 1476
CfoI GCGC 3 cut(s) 129, 241, 1771
Cfr10I RCCGGY 5 cut(s) 250, 540, 1152, 1238, 1726
Cfr13I GGNCC 4 cut(s) 856, 896, 1135, 1150
CseI GACGC 1 cut(s) 993
Csp6I GTAC 3 cut(s) 511, 1224, 1285
CspAI ACCGGT 2 cut(s) 250, 540
CviAII CATG 2 cut(s) 632, 1599
CviQI GTAC 3 cut(s) 511, 1224, 1285
DdeI CTNAG 4 cut(s) 28, 83, 120, 579
DpnI GATC 6 cut(s) 177, 332, 789, 892, 1077, 1631
DpnII GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
EaeI YGGCCR 1 cut(s) 1732
Eam1104I CTCTTC 4 cut(s) 47, 157, 920, 1183
EarI CTCTTC 4 cut(s) 47, 157, 920, 1183
Eco130I CCWWGG 3 cut(s) 859, 1093, 1711
Eco147I AGGCCT 1 cut(s) 487
Eco24I GRGCYC 1 cut(s) 1378
Eco31I GGTCTC 3 cut(s) 223, 779, 1399
Eco47I GGWCC 3 cut(s) 856, 896, 1135
Eco57I CTGAAG 4 cut(s) 138, 380, 671, 1426
EcoO109I RGGNCCY 1 cut(s) 1135
EcoRII CCWGG 2 cut(s) 324, 1734
EcoT14I CCWWGG 3 cut(s) 859, 1093, 1711
EcoT38I GRGCYC 1 cut(s) 1378
ErhI CCWWGG 3 cut(s) 859, 1093, 1711
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 2 cut(s) 635, 1602
FaqI GGGAC 5 cut(s) 440, 743, 1121, 1135, 1637
FatI CATG 2 cut(s) 631, 1598
FauI CCCGC 4 cut(s) 400, 762, 939, 1318
FauNDI CATATG 1 cut(s) 981
FbaI TGATCA 1 cut(s) 1629
FblI GTMKAC 1 cut(s) 601
Fnu4HI GCNGC 4 cut(s) 194, 279, 744, 1606
FokI GGATG 9 cut(s) 30, 272, 413, 752, 938, 1346, 1580, 1687, 1704
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 4 cut(s) 194, 279, 744, 1606
FspBI CTAG 2 cut(s) 197, 1524
GlaI GCGC 3 cut(s) 128, 240, 1770
GluI GCNGC 4 cut(s) 194, 279, 744, 1606
GsaI CCCAGC 1 cut(s) 285
GsuI CTGGAG 1 cut(s) 768
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 3 cut(s) 487, 1152, 1734
HgaI GACGC 1 cut(s) 993
HhaI GCGC 3 cut(s) 129, 241, 1771
Hin1I GRCGYC 1 cut(s) 1004
Hin1II CATG 2 cut(s) 635, 1602
Hin6I GCGC 3 cut(s) 127, 239, 1769
HinP1I GCGC 3 cut(s) 127, 239, 1769
HindIII AAGCTT 2 cut(s) 80, 1202
HinfI GANTC 6 cut(s) 445, 529, 752, 841, 1384, 1511
HphI GGTGA 3 cut(s) 902, 1540, 1684
Hpy166II GTNNAC 3 cut(s) 602, 856, 1069
Hpy188I TCNGA 8 cut(s) 342, 386, 429, 582, 651, 1062, 1080, 1445
Hpy8I GTNNAC 3 cut(s) 602, 856, 1069
Hpy99I CGWCG 1 cut(s) 1319
HpyAV CCTTC 8 cut(s) 60, 355, 541, 646, 651, 700, 944, 1009
HpyCH4III ACNGT 2 cut(s) 522, 1105
HpyCH4IV ACGT 1 cut(s) 1626
HpyCH4V TGCA 8 cut(s) 21, 204, 248, 618, 703, 966, 1296, 1365
HpyF10VI GCNNNNNNNGC 6 cut(s) 245, 484, 1280, 1343, 1475, 1595
HpyF3I CTNAG 4 cut(s) 28, 83, 120, 579
HpySE526I ACGT 1 cut(s) 1626
Hsp92I GRCGYC 1 cut(s) 1004
Hsp92II CATG 2 cut(s) 635, 1602
HspAI GCGC 3 cut(s) 127, 239, 1769
KflI GGGWCCC 1 cut(s) 1135
Kpn2I TCCGGA 2 cut(s) 463, 893
Ksp22I TGATCA 1 cut(s) 1629
Kzo9I GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
LmnI GCTCC 4 cut(s) 310, 766, 1373, 1602
Lsp1109I GCAGC 3 cut(s) 180, 265, 1617
LweI GCATC 5 cut(s) 8, 730, 1324, 1456, 1558
MaeI CTAG 2 cut(s) 197, 1524
MaeII ACGT 1 cut(s) 1626
MaeIII GTNAC 4 cut(s) 179, 253, 500, 1744
MalI GATC 6 cut(s) 177, 332, 789, 892, 1077, 1631
MboI GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
MboII GAAGA 7 cut(s) 34, 144, 325, 377, 907, 1200, 1585
MflI RGATCY 3 cut(s) 330, 787, 890
MhlI GDGCHC 1 cut(s) 1378
MlsI TGGCCA 1 cut(s) 1734
MluCI AATT 9 cut(s) 467, 606, 970, 992, 1021, 1254, 1304, 1328, 1457
MluNI TGGCCA 1 cut(s) 1734
MlyI GAGTC 2 cut(s) 761, 1505
MmeI TCCRAC 1 cut(s) 849
Mox20I TGGCCA 1 cut(s) 1734
MroI TCCGGA 2 cut(s) 463, 893
MroXI GAANNNNTTC 1 cut(s) 1203
MscI TGGCCA 1 cut(s) 1734
Msp20I TGGCCA 1 cut(s) 1734
MspCI CTTAAG 1 cut(s) 299
MspR9I CCNGG 4 cut(s) 326, 1119, 1540, 1736
Mva1269I GAATGC 2 cut(s) 589, 1093
MvaI CCWGG 2 cut(s) 326, 1736
MvnI CGCG 3 cut(s) 407, 769, 1073
MwoI GCNNNNNNNGC 6 cut(s) 245, 484, 1280, 1343, 1475, 1595
NciI CCSGG 2 cut(s) 1119, 1540
NdeI CATATG 1 cut(s) 981
NdeII GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
NlaIII CATG 2 cut(s) 635, 1602
NmeAIII GCCGAG 1 cut(s) 146
PacI TTAATTAA 1 cut(s) 1257
PceI AGGCCT 1 cut(s) 487
PctI GAATGC 2 cut(s) 589, 1093
PdmI GAANNNNTTC 1 cut(s) 1203
PfeI GAWTC 4 cut(s) 445, 529, 841, 1384
PflMI CCANNNNNTGG 1 cut(s) 136
PinAI ACCGGT 2 cut(s) 250, 540
PkrI GCNGC 4 cut(s) 195, 280, 745, 1607
PleI GAGTC 2 cut(s) 760, 1505
PpsI GAGTC 2 cut(s) 760, 1505
PpuMI RGGWCCY 1 cut(s) 1135
PshBI ATTAAT 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 1135
Psp6I CCWGG 2 cut(s) 324, 1734
PspFI CCCAGC 1 cut(s) 281
PspGI CCWGG 2 cut(s) 324, 1734
PspPI GGNCC 4 cut(s) 856, 896, 1135, 1150
PspPPI RGGWCCY 1 cut(s) 1135
PstI CTGCAG 1 cut(s) 968
PsuI RGATCY 3 cut(s) 330, 787, 890
RsaI GTAC 3 cut(s) 512, 1225, 1286
RsaNI GTAC 3 cut(s) 511, 1224, 1285
SatI GCNGC 4 cut(s) 194, 279, 744, 1606
Sau3AI GATC 6 cut(s) 175, 330, 787, 890, 1075, 1629
Sau96I GGNCC 4 cut(s) 856, 896, 1135, 1150
ScaI AGTACT 1 cut(s) 1225
SchI GAGTC 2 cut(s) 761, 1505
ScrFI CCNGG 4 cut(s) 326, 1119, 1540, 1736
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 5 cut(s) 8, 730, 1324, 1456, 1558
SfcI CTRYAG 1 cut(s) 964
SinI GGWCC 3 cut(s) 856, 896, 1135
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 9 cut(s) 467, 606, 970, 992, 1021, 1254, 1304, 1328, 1457
SseBI AGGCCT 1 cut(s) 487
SspMI CTAG 2 cut(s) 197, 1524
StuI AGGCCT 1 cut(s) 487
StyD4I CCNGG 4 cut(s) 324, 1117, 1538, 1734
StyI CCWWGG 3 cut(s) 859, 1093, 1711
TaaI ACNGT 2 cut(s) 522, 1105
TaiI ACGT 1 cut(s) 1629
TaqI TCGA 4 cut(s) 221, 413, 1157, 1559
TaqII GACCGA 1 cut(s) 205
TasI AATT 9 cut(s) 467, 606, 970, 992, 1021, 1254, 1304, 1328, 1457
TatI WGTACW 1 cut(s) 1223
TauI GCSGC 1 cut(s) 746
TfiI GAWTC 4 cut(s) 445, 529, 841, 1384
TscAI CASTG 2 cut(s) 1042, 1771
TseI GCWGC 3 cut(s) 193, 278, 1605
TspDTI ATGAA 4 cut(s) 245, 632, 1376, 1547
TspGWI ACGGA 4 cut(s) 105, 272, 767, 1517
TspRI CASTG 2 cut(s) 1042, 1771
Van91I CCANNNNNTGG 1 cut(s) 136
Vha464I CTTAAG 1 cut(s) 299
VpaK11BI GGWCC 3 cut(s) 856, 896, 1135
VspI ATTAAT 1 cut(s) 207
XapI RAATTY 6 cut(s) 467, 606, 970, 992, 1304, 1457
XmiI GTMKAC 1 cut(s) 601
XmnI GAANNNNTTC 1 cut(s) 1203
XspI CTAG 2 cut(s) 197, 1524
ZrmI AGTACT 1 cut(s) 1225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.